Click chemistry in Applications

Copper-catalyzed reaction of an azide with an alkyne to form a 5-membered heteroatom ring, also known as Cu(I)-Catalyzed Azide-Alkyne Cycloaddition (CuAAC), is a powerful reaction for the synthesis of bioconjugates.

Scheme of copper catalyzed Click chemistry alkyne azide cycloaddition

Terminal alkyne and azide groups are almost never encountered in natural molecules, so they must be first introduced into the biomolecules to be linked. Alkyne-containing DNA can be prepared with an alkyne phosphoramidite during standard oligo synthesis. Azide or alkyne groups can be attached to proteins by using bifunctional adapter molecules with an NHS ester moiety.

Aqueous DMSO, DMF, acetonitrile, alcohols, or pure water and buffers can be used for the Click chemistry reaction. The reaction is compatible with a wide range of functional groups.

The following list includes only a few of reagents for Click chemistry. Please check other categories, especially alkynes and azides.

Ascorbic acid

Ascorbic acid for the reduction of Cu(II)-TBTA complex.
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12050 10 mg $20.00 in stock
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Azide-PEG3-amine

A bifunctional linker with azide and amine group flanking PEG3 (triethylene glycol).
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218-1g 1 g $95.00 in stock
218-5g 5 g $280.00 in stock
218-25g 25 g please inquire 21 days
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Azide-PEG3-azide

Triethylene glycol diazide is a bifunctional crosslinker bearing two azide groups. Suitable for Click chemistry.
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207-1g 1 g $95.00 in stock
207-5g 5 g $390.00 in stock
207-25g 25 g please inquire 21 days
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BDP 558/568 azide

BDP 558/568 is a borondipyrromethene fluorophore with absorption and emission spectra close to Cyanine3. This is an azide derivative for Click chemistry.
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17430 1 mg $110.00 in stock
27430 5 mg $210.00 in stock
37430 10 mg $310.00 in stock
47430 25 mg $410.00 in stock
57430 50 mg $695.00 in stock
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Click chemistry buffer

Catalytic buffer for Click chemistry modification of nucleic acids and small molecules.
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61150 55 mL $49.00 5 days
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Copper(II)-TBTA complex, 10 mM in 55% aq. DMSO

Catalyst for bioconjugate synthesis by Click Chemistry.
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21050 1.5 mL $55.00 in stock
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DMSO, labeling grade

Pure dimethyl sulfoxide (DMSO) solvent with quality optimised for Click and other cojugation reactions
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15050 1 mL $10.00 in stock
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THPTA ligand

THPTA is a water soluble ligand for Cu(I) catalyzed Click chemistry. The ligand stabilizes copper in its Cu(I) oxidation state. Due to high aqueous solubility, reactions with this ligand do not require an organic co-solvent.
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Cat. # Quantity Price Lead time
F4050 100 mg $50.00 in stock
H4050 500 mg $250.00 5 days
I4050 1 g $370.00 5 days
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