Copper-catalyzed reaction of an azide with an alkyne to form a 5-membered heteroatom ring, also known as Cu(I)-Catalyzed Azide-Alkyne Cycloaddition (CuAAC), is a powerful reaction for the synthesis of bioconjugates.
Terminal alkyne and azide groups are almost never encountered in natural molecules, so they must be first introduced into the biomolecules to be linked. Alkyne-containing DNA can be prepared with an alkyne phosphoramidite during standard oligo synthesis. Azide or alkyne groups can be attached to proteins by using bifunctional adapter molecules with an NHS ester moiety.
Aqueous DMSO, DMF, acetonitrile, alcohols, or pure water and buffers can be used for the сlick chemistry reaction. The reaction is compatible with a wide range of functional groups.
The following list includes only a few of reagents for сlick chemistry. Please check other categories, especially alkynes and azides .
An azide-functionalized monosaccharide for metabolic labeling and studying the cellular trafficking of labeled proteins and proteomic analysis.
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3662-5mg
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Water-soluble, bifunctional short crosslinker with amino and azide groups.
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58-1g
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A bifunctional short linker with amino and azido groups. A solid, chemically stable hydrochloride form of azidopropylamine. This product is ideal as an azidopropylamine bulk for applications when a large amount of reagent is needed.
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An azide-functionalized monosaccharide for metabolic labeling and studying the cellular trafficking of labeled proteins and proteomic analysis.
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3706-5mg
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An alkyne-functionalized monosaccharide for metabolic labeling and studying the cellular trafficking of labeled proteins and proteomic analysis.
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3431-5mg
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An azide-functionalized monosaccharide for metabolic labeling and studying the cellular trafficking of labeled proteins and proteomic analysis.
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3159-5mg
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Alkyne derivative of yellow-green fluorescent dye AF 430 for copper-catalyzed click reaction with azides.
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Azide derivative of AF 430 for labeling and detecting alkyne-containing biomolecules via сlick reaction.
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A0830
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Alkyne derivative of AF 488 dye for copper-catalyzed cycloaddition to azides. Pure 5-isomer.
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AF 488 azide is a fluorophore with emission in the green spectrum range. It reacts with alkynes in copper-catalyzed cycloaddition reactions.
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Azide derivative of yellow fluorescent dye AF 555 for сlick сhemistry.
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1K830
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AF 568 derivative designed for fluorescence microscopy and flow cytometry. Pure 5-isomer.
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Azide derivative of water-soluble AF 568 dye for labeling alkyne-containing biomolecules and fluorescent visualization of biological objects. Pure 6-isomer.
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Azide derivative of water-soluble red-fluorescent dye AF 594 for labeling alkyne-containing biomolecules and fluorescent visualization of biological objects. Pure 6-isomer.
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A7830
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Bright and photostable azide AF 647 for labeling alkyne-containing biomolecules and fluorescent visualization of biological objects.
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A6830
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Acetylenic amidite for the incorporation of acetylene groups into oligos.
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Controlled pore glass solid support for DNA/RNA synthesis with alkyne modification (pore size 1000 Å) for the synthesis of long modified oligonucleotides with 3'-alkyne modification.
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Solid support for the synthesis of 3'-modified oligonucleotides with alkyne group for Click Chemistry.
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Phosphoramidite modifier for the synthesis of oligonucleotides containing alkyne modification in the middle of the chain.
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1897-100mg
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Alkyne dT phosphoramidite is a modifier nucleoside amidite for the synthesis of alkynylated oligonucleotides containing an internal label.
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Bifunctional reagent containing alkyne group and hydrazide function. It reacts with aldehydes and ketones. Alkyne group can then be coupled with various azides via CuAAC reaction.
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Maleimide reagent for the attachment of alkyne functional group to proteins, peptides, and other molecules bearing SH (thiol) groups, including cysteine residues.
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Alkyne phosphoramidite for the synthesis of oligonucleotides ready for click chemistry.
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Diethyleneglycol (PEG2) based bifunctional linker containing amine and alkyne functional groups.
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Bifunctional diethyleneglycol linker with terminal alkyne and carboxylic acid moieties.
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Bifunctional reactive crosslinker containing terminal alkyne group for click chemistry or Sonogashira coupling, and reactive alkyl iodide group.
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Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on diethyleneglycol (PEG2).
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2219-1g
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Alkyne-PEG3-amine is a medium-length heterobifunctional linker for conjugation.
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Bifunctional triethyleneglycol linker with terminal alkyne and carboxylic acid moieties.
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PEG3 with alkyne and iodide functional groups, a reactive and hydrophilic linker for alkylation and click chemistry.
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Bifunctional spacer with alkyne and maleimide groups.
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Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on triethyleneglycol (PEG3).
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Alkyne-PEG3-OH is a triethyleneglycol based bifunctional linker with a hydroxyl group and an alkyne fragment.
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A bifunctional linker molecule containing both terminal alkyne moiety for click chemistry, and amine group. Contains a long hydrophilic PEG4 arm.
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2025-100mg
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Bifunctional tetraethyleneglycol linker with terminal alkyne and carboxylic acid moieties.
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Bifunctional reactive PEG4-based crosslinker containing terminal alkyne group for click chemistry or Sonogashira coupling and reactive alkyl iodide group.
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Water soluble, heterobifunctional hydrophilic linker with a length of 14 Å, containing maleimide for thiol labeling, and alkyne for click chemistry. An ideal reagent to attach an alkyne moiety to a protein.
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2002-100mg
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Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on tetraethyleneglycol (PEG4).
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Alkyne phosphoramidite for conjugation of oligonucleotides with biomolecules.
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2779-100mg
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