Click chemistry in Applications

Copper-catalyzed reaction of an azide with an alkyne to form a 5-membered heteroatom ring, also known as Cu(I)-Catalyzed Azide-Alkyne Cycloaddition (CuAAC), is a powerful reaction for the synthesis of bioconjugates.

Scheme of copper catalyzed Click chemistry alkyne azide cycloaddition

Terminal alkyne and azide groups are almost never encountered in natural molecules, so they must be first introduced into the biomolecules to be linked. Alkyne-containing DNA can be prepared with an alkyne phosphoramidite during standard oligo synthesis. Azide or alkyne groups can be attached to proteins by using bifunctional adapter molecules with an NHS ester moiety.

Aqueous DMSO, DMF, acetonitrile, alcohols, or pure water and buffers can be used for the Click chemistry reaction. The reaction is compatible with a wide range of functional groups.

The following list includes only a few of reagents for Click chemistry. Please check other categories, especially alkynes and azides.

Ascorbic acid

Ascorbic acid for the reduction of Cu(II)-TBTA complex.
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Cat. # Quantity Price Lead time
12050 10 mg $20.00 in stock


A bifunctional linker with azide and amine group flanking PEG3 (triethylene glycol).
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Cat. # Quantity Price Lead time
218-1g 1 g $95.00 in stock
218-5g 5 g $280.00 21 days
218-25g 25 g please inquire 21 days


Triethylene glycol diazide is a bifunctional crosslinker bearing two azide groups. Suitable for Click chemistry.
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Cat. # Quantity Price Lead time
207-1g 1 g $95.00 in stock
207-5g 5 g $390.00 5 days
207-25g 25 g please inquire 10 days

Copper(II)-TBTA complex, 10 mM in 55% aq. DMSO

Catalyst for bioconjugate synthesis by Click Chemistry.
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Cat. # Quantity Price Lead time
21050 1.5 mL $55.00 in stock

THPTA ligand

THPTA is a water soluble ligand for Cu(I) catalyzed Click chemistry. The ligand stabilizes copper in its Cu(I) oxidation state. Due to high aqueous solubility, reactions with this ligand do not require an organic co-solvent.
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Cat. # Quantity Price Lead time
F4050 100 mg $50.00 in stock
H4050 500 mg $250.00 in stock
I4050 1 g $370.00 7 days

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