Click chemistry in Applications

The typical click chemistry reaction is the Copper-catalyzed reaction of an azide with an alkyne to form a 5-membered heteroatom ring, also known as Cu(I)-Catalyzed Azide-Alkyne Cycloaddition (CuAAC). The azide and alkyne functional groups must first be introduced into the biomolecules to be linked. Alkyne-containing DNA can be prepared with an alkyne phosphoramidite during standard oligo synthesis. Azide or alkyne groups can be attached to proteins by using bifunctional adapter molecules with an NHS ester moiety. Aqueous DMSO, DMF, acetonitrile, alcohols, or pure water and buffers can be used for the reaction. The reaction is biocompatible and can take place in living cells.

Ascorbic acid

Ascorbic acid for the reduction of Cu(II)-TBTA complex.
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Cat. # Quantity Price Lead time
12050 10 mg $20.00 5 days

Copper(II)-TBTA complex, 10 mM in 55% aq. DMSO

Catalyst for bioconjugate synthesis by Click Chemistry.
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Cat. # Quantity Price Lead time
21050 1.5 mL $55.00 stock

DMF (dimethylformamide), labeling grade

Dry high purity amine free solvent for NHS ester labeling.
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Cat. # Quantity Price Lead time
13050 1 mL $10.00 stock

Sulfo-Cyanine7.5 azide

Sulfo-Cyanine7.5 is a very hydrophilic and water soluble dye for the near infrared region. The absorption and emission spectra of the dye are similar to indocyanine green (ICG), but sulfo-Cyanine7.5 has a much higher fluorescence quantum yield. The dye is a great choice for near infrared in vivo imaging. This is an azide for Click chemistry.
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Cat. # Quantity Price Lead time
A6330 1 mg $110.00 stock
B6330 5 mg $290.00 stock
C6330 10 mg $470.00 stock
D6330 25 mg $690.00 stock
E6330 50 mg $1270.00 stock
F6330 100 mg $1990.00 stock

THPTA ligand

THPTA is a water soluble ligand for Cu(I) catalyzed Click chemistry. The ligand stabilizes copper in its Cu(I) oxidation state. Due to high aqueous solubility, reactions with this ligand do not require an organic co-solvent.
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Cat. # Quantity Price Lead time
F4050 100 mg $50.00 stock
H4050 500 mg $250.00 stock
I4050 1 g $370.00 stock

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