Copper-catalyzed reaction of an azide with an alkyne to form a 5-membered heteroatom ring, also known as Cu(I)-Catalyzed Azide-Alkyne Cycloaddition (CuAAC), is a powerful reaction for the synthesis of bioconjugates.
Terminal alkyne and azide groups are almost never encountered in natural molecules, so they must be first introduced into the biomolecules to be linked. Alkyne-containing DNA can be prepared with an alkyne phosphoramidite during standard oligo synthesis. Azide or alkyne groups can be attached to proteins by using bifunctional adapter molecules with an NHS ester moiety.
Aqueous DMSO, DMF, acetonitrile, alcohols, or pure water and buffers can be used for the сlick chemistry reaction. The reaction is compatible with a wide range of functional groups.
The following list includes only a few of reagents for сlick chemistry. Please check other categories, especially alkynes and azides.
Degrader building block containing an E3 ligase ligand with linker and azide group, which can be conjugated to alkyne-functionalized linkers and target protein ligands through click chemistry reactions.
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Water-soluble, bifunctional short crosslinker with amino and azide groups.
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A bifunctional short linker with amino and azido groups. A solid, chemically stable hydrochloride form of azidopropylamine. This product is ideal as an azidopropylamine bulk for applications when a large amount of reagent is needed.
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Unnatural L-phenylalanine derivative with an azide moiety for nascent protein synthesis detection via copper-catalyzed and copper-free click chemistry.
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Uridine alkyne derivative for monitoring and investigation of RNA transcription in cells.
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An alkyne-functionalized monosaccharide for metabolic labeling and studying the cellular trafficking of labeled proteins and proteomic analysis.
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An azide-functionalized monosaccharide for metabolic labeling and studying the cellular trafficking of labeled proteins and proteomic analysis.
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An alkyne-functionalized monosaccharide for metabolic labeling and studying the cellular trafficking of labeled proteins and proteomic analysis.
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An azide-functionalized monosaccharide for metabolic labeling and studying the cellular trafficking of labeled proteins and proteomic analysis.
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An alkyne-functionalized monosaccharide for metabolic labeling and studying the cellular trafficking of labeled proteins and proteomic analysis.
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An azide-functionalized monosaccharide for metabolic labeling and studying the cellular trafficking of labeled proteins and proteomic analysis.
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AF 343 (Coumarin) is a blue emitting fluorophore that can serve as a FRET donor for fluorescein (FAM). This azide derivative can be conjugated with alkynes by сlick chemistry. An extended linker arm between the fluorophore and the azide group enhances solubility and provides spatial separation between the dye and the biomolecule.
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PEP (phenylethynylpyrene), polyaromatic hydrocarbon fluorescent dye for click-chemistry labeling.
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Alkyne derivative of yellow-green fluorescent dye AF 430 for copper-catalyzed click reaction with azides.
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Azide derivative of AF 430 for labeling and detecting alkyne-containing biomolecules via сlick reaction.
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Alkyne derivative of AF 488 dye for copper-catalyzed cycloaddition to azides. Pure 5-isomer.
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AF 488 azide is a fluorophore with emission in the green spectrum range. It reacts with alkynes in copper-catalyzed cycloaddition reactions.
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Azide derivative of yellow fluorescent dye AF 555 for сlick сhemistry.
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AF 568 derivative designed for fluorescence microscopy and flow cytometry. Pure 5-isomer.
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Azide derivative of water-soluble AF 568 dye for labeling alkyne-containing biomolecules and fluorescent visualization of biological objects. Pure 6-isomer.
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Azide derivative of water-soluble red-fluorescent dye AF 594 for labeling alkyne-containing biomolecules and fluorescent visualization of biological objects. Pure 6-isomer.
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Bright and photostable azide AF 647 for labeling alkyne-containing biomolecules and fluorescent visualization of biological objects.
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Noncanonical amino acid analog of methionine with an azide moiety for nascent protein synthesis detection via copper-catalyzed and copper-free click chemistry.
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Acetylenic amidite for the incorporation of acetylene groups into oligos.
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Controlled pore glass solid support for DNA/RNA synthesis with alkyne modification (pore size 1000 Å) for synthesizing long-modified oligonucleotides with 3'-alkyne modification.
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Solid support for the synthesis of 3'-modified oligonucleotides with alkyne group for Click Chemistry.
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Phosphoramidite modifier for synthesizing oligonucleotides containing alkyne modification in the middle of the chain.
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Alkyne dT phosphoramidite is a modifier nucleoside amidite for the synthesis of alkynylated oligonucleotides containing an internal label.
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Bifunctional reagent containing alkyne group and hydrazide function. It reacts with aldehydes and ketones. Alkyne group can then be coupled with various azides via CuAAC reaction.
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Maleimide reagent for the attachment of alkyne functional group to proteins, peptides, and other molecules bearing SH (thiol) groups, including cysteine residues.
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Alkyne phosphoramidite for the synthesis of oligonucleotides ready for click chemistry.
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Diethyleneglycol (PEG2) based bifunctional linker containing amine and alkyne functional groups.
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Bifunctional diethyleneglycol linker with terminal alkyne and carboxylic acid moieties.
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Bifunctional reactive crosslinker containing terminal alkyne group for click chemistry or Sonogashira coupling, and reactive alkyl iodide group.
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Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on diethyleneglycol (PEG2).
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Alkyne-PEG3-amine is a medium-length heterobifunctional linker for conjugation.
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Bifunctional triethyleneglycol linker with terminal alkyne and carboxylic acid moieties.
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PEG3 with alkyne and iodide functional groups, a reactive and hydrophilic linker for alkylation and click chemistry.
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Bifunctional spacer with alkyne and maleimide groups for conjugation of biomolecules.
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Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on triethyleneglycol (PEG3).
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Alkyne-PEG3-OH is a triethyleneglycol based bifunctional linker with a hydroxyl group and an alkyne fragment.
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A bifunctional linker molecule containing both terminal alkyne moiety for click chemistry, and amine group. Contains a long hydrophilic PEG4 arm.
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Bifunctional tetraethyleneglycol linker with terminal alkyne and carboxylic acid moieties.
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Bifunctional reactive PEG4-based crosslinker containing terminal alkyne group for click chemistry or Sonogashira coupling and reactive alkyl iodide group.
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Water soluble, heterobifunctional hydrophilic linker with a length of 14 Å, containing maleimide for thiol labeling, and alkyne for click chemistry. An ideal reagent to attach an alkyne moiety to a protein.
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Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on tetraethyleneglycol (PEG4).
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Alkyne phosphoramidite for conjugation of oligonucleotides with biomolecules.
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Azide derivative of AMCA fluorescent dye for сlick сhemistry. AMCA is one of the brightest blue fluorescent dyes with large Stoke's shift and high resistance to photobleaching.
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Ascorbic acid is a reducing agent converting copper(II) into a monovalent form that catalyzes the click reaction.
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N-Succinimidyl 4-Azidotetrafluorobenzoate (ATFB SE, ATFB-OSu) is a photo-activatable linker used as a versatile labeling agent for investigating biological receptors.
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Azide derivative of water-soluble yellow-fluorescent dye ATT 550 for labeling alkyne-containing biomolecules and fluorescent visualization of biological objects.
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Azide derivative of water-soluble orange-fluorescent dye ATT 565 for labeling alkyne-containing biomolecules and fluorescent visualization of biological objects. Pure 6-isomer.
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Azide derivative of water-soluble red fluorescent dye ATT 594 for labeling alkyne-containing biomolecules and fluorescent visualization of biological objects.
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Azide derivative of water-soluble far-red fluorescent dye ATT 633 for labeling alkyne-containing biomolecules and fluorescent visualization of biological objects.
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A diethylene glycol (PEG2) linker with azide and amino groups.
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Bifunctional diethylene glycol with terminal carboxyl and azido groups.
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A bifunctional molecule containing hydrophilic PEG2 with azide and iodide groups.
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A bifunctional linker with azide and amine groups flanking PEG3 (triethylene glycol)
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Triethylene glycol diazide is a bifunctional crosslinker bearing two azide groups. Suitable for click chemistry.
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Bifunctional triethylene glycol with terminal carboxyl and azido groups.
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Azide-PEG3-chloride contains a reactive halogen atom and an azide group for click chemistry reaction. It can also serve as a protected amine group equivalent.
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Azide-PEG3-iodide is a bifunctional linker with a highly reactive alkylative halogen functional group and an azido group for click chemistry or Staudinger ligation.
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Azide-PEG3-OH is a bifunctional molecule with an azide group, primary alcohol, and a hydrophilic PEG3 linker in between.
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A hydrophilic bifunctional linker with azide and tetrazine function groups flanking PEG3 (triethylene glycol) spacer.
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PEG4 amine azide is a bifunctional linker with amine and azide functional groups. It is based on a tetraethyleneglycol core. The length of the PEG4 linker is 1.4 nm.
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PEG4 diazide is a homobifunctional linker with two azide groups. It can be used for the synthesis of dimeric molecules with a hydrophilic core by click chemistry.
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Bifunctional tetraethylene glycol with terminal carboxyl and azido groups.
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A bifunctional reagent containing the azide group and hydrazide function. It reacts with aldehydes and ketones. The azide group can then be coupled with various alkynes via CuAAC reaction.
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Azide-PEG4-OH is a bifunctional molecule with an azide group, primary alcohol, and a hydrophilic PEG4 linker in between.
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A hydrophilic bifunctional linker with azide and NHS ester function groups flanking PEG6 (hexaethylene glycol).
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Water-soluble heterobifunctional crosslinker with a primary hydroxyl group, alkyne-reactive azide group, and a PEG6 hydrophilic linker.
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BDP 558/568 is a borondipyrromethene dye for Cyanine3-channel. This product is a terminal alkyne derivative for copper-catalyzed click chemistry.
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BDP 558/568 is a borondipyrromethene fluorophore with absorption and emission spectra close to Cyanine3. This is an azide derivative for сlick chemistry.
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Azide derivative of BDP 576/589 dye for labeling and detecting alkyne-containing biomolecules via сlick reaction.
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BDP 581/591 is a borondipyrromethene fluorophore useful for fluorescence polarization assays, microscopy, and two-photon experiments. This alkyne is a derivative for copper-catalyzed click chemistry.
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BDP 581/591 is a relatively hydrophobic borondipyrromethene fluorophore that can be used as a probe for ROS species, or fluorescence polarization tracer. This is an azide derivative for сlick chemistry.
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Alkyne dye for copper-catalyzed click chemistry, containing borondipyrromethene fluorophore BDP 630/650 for the red channel.
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BDP 630/650 is a bright borondipyrromethene-based fluorophore for the far red area of the spectrum. It possesses a high extinction coefficient and quantum yield. This is an azide for сlick chemistry labeling.
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BDP 650/665 is a bright borondipyrromethene red-emitting dye. The terminal ethynyl group of BDP 650/665 alkyne can be easily conjugated with various azides using a copper-catalyzed click reaction.
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BDP 650/665 is a red-emitting borondipyrromethene dye. This azide derivative can be conjugated with alkynes and cycloalkynes.
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Borondipyrromethene dye alkyne for click chemistry. Bright and photostable dye for FAM channel.
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BDP FL is a bright and photostable borondipyrromethene dye for the fluorescein channel. This is a dye azide for сlick сhemistry.
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Terminal alkyne derivative of BDP R6G dye for сlick chemistry. BDP R6G is a borondipyrromethene dye whose absorption and emission spectra are similar to R6G (rhodamine 6G).
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BDP R6G is a high performance borondipyrromethene fluorophore which is tuned to match excitation and emission channels of R6G dye.
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Borondipyrromethene dye for TAMRA channel, with alkyne group for copper-catalyzed click chemistry.
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Borondipyrromethene dye with azido group. Spectral properties are similar to TAMRA dye. Due to long fluorescence lifetime, BDP TMR is especially useful for fluorescence polarization measurements.
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BDP TR is a fluorophore for ROX channel. This borondipyrromethene dye is very bright, and resistant to oxidation.
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BDP TR is a red-emitting fluorophore for the ROX channel. This is an azide for copper-catalyzed and copper-free сlick chemistry conjugation.
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Biotin alkyne is a reactive affinity label which can be attached to biomolecules via click reaction.
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Biotin alkyne is a reagent for click chemistry labeling with biotin, an affinity probe to streptavidin.
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Biotin azide is a reagent for click chemistry labeling with biotin, a well-known affinity probe to streptavidin.
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Biotin azide is a reagent for click chemistry labeling with biotin, a well-known affinity probe to streptavidin.
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Biotin alkyne is a reagent for click chemistry labeling with biotin, an affinity probe to streptavidin.
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Biotin azide is a reagent for click chemistry labeling with biotin, a well-known affinity probe to streptavidin.
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BTTAA is the newest generation of water-soluble ligands for the copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) that dramatically accelerates reaction rates and suppresses cell cytotoxicity.
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C8-Alkyne-dT-CE-phosphoramidite is a specialized reagent used in the synthesis of oligonucleotides that incorporates a single dT alkyne group. This compound is particularly valuable for applications involving click chemistry, allowing for efficient conjugation reactions.
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Cholesterol derivative with azide group for сlick chemistry conjugation.
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A catalyst for copper-catalyzed click reactions in aqueous media. A stable сopper(II) complex with BTTAA which must be converted to copper(I) using reducing agents. The complex can be used to prepare a reaction buffer of any composition.
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A catalyst for click reaction. A stable copper(II) complex which must be converted to monovalent copper(I) in situ using reducing agents. The complex can be used to prepare a reaction buffer of any composition.
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Ready-to-use catalytic buffer containing сopper(II) and water-soluble THPTA ligand. It is suitable for click chemistry modification of proteins.
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