Click chemistry in Applications

Copper-catalyzed reaction of an azide with an alkyne to form a 5-membered heteroatom ring, also known as Cu(I)-Catalyzed Azide-Alkyne Cycloaddition (CuAAC), is a powerful reaction for the synthesis of bioconjugates.

Scheme of copper-catalyzed сlick chemistry alkyne azide cycloaddition

Terminal alkyne and azide groups are almost never encountered in natural molecules, so they must be first introduced into the biomolecules to be linked. Alkyne-containing DNA can be prepared with an alkyne phosphoramidite during standard oligo synthesis. Azide or alkyne groups can be attached to proteins by using bifunctional adapter molecules with an NHS ester moiety.

Aqueous DMSO, DMF, acetonitrile, alcohols, or pure water and buffers can be used for the сlick chemistry reaction. The reaction is compatible with a wide range of functional groups.

The following list includes only a few of reagents for сlick chemistry. Please check other categories, especially alkynes and azides.

3-Azidopropylamine

Bifunctional short crosslinker with an aminogroup and azide group.
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Cat. # Quantity Price Lead time
58-1g 1 g $145.00 in stock
58-5g 5 g $490.00 in stock

Ac4ManNAz (N-Azidoacetylmannosamine-tetraacylated)

An azide-functionalized monosaccharide for metabolic labeling and studying the cellular trafficking of labeled proteins and proteomic analysis.
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Cat. # Quantity Price Lead time
3159-5mg 5 mg $45.00 in stock
3159-25mg 25 mg $190.00 in stock
3159-100mg 100 mg $580.00 in stock

Ascorbic acid

Ascorbic acid for the reduction of Copper(II)-TBTA complex complex.
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Cat. # Quantity Price Lead time
12050 10 mg $20.00 in stock

Azide-PEG3-Amine

A bifunctional linker with azide and amine groups flanking PEG3 (triethylene glycol)
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Cat. # Quantity Price Lead time
218-1g 1 g $95.00 in stock
218-5g 5 g $280.00 in stock
218-25g 25 g please inquire in stock

Azide-PEG3-Azide

Triethylene glycol diazide (CAS# 59559-06-7) is a bifunctional crosslinker bearing two azide groups. Suitable for click chemistry.
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Cat. # Quantity Price Lead time
207-1g 1 g $95.00 in stock
207-5g 5 g $390.00 in stock
207-25g 25 g please inquire in stock

Azide-PEG4-Amine

PEG4 amine azide (CAS# 134179-38-7) is a bifunctional linker with amine and azide functional groups. It is based on a tetraethyleneglycol core. The length of the PEG4 linker is 1.4 nm.
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Cat. # Quantity Price Lead time
1868-1g 1 g $90.00 in stock
1868-5g 5 g $250.00 in stock
1868-25g 25 g $950.00 in stock

Azide-PEG4-Azide

PEG4 diazide (CAS# 101187-39-7) is a homobifunctional linker with two azide groups. It can be used for the synthesis of dimeric molecules with a hydrophilic core by Click chemistry.
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Cat. # Quantity Price Lead time
1865-1g 1 g $90.00 in stock
1865-5g 5 g $250.00 in stock
1865-25g 25 g $950.00 in stock

BTTAA ligand

BTTAA is the newest generation of water-soluble ligands for the copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) that dramatically accelerates reaction rates and suppresses cell cytotoxicity.
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Cat. # Quantity Price Lead time
3253-100mg 100 mg $149.00 in stock
3253-500mg 500 mg $560.00 in stock
3253-1g 1 g $990.00 in stock
3253-5g 5 g please inquire 10 days

Click chemistry buffer, 1.5x

Catalytic buffer for click chemistry modification of nucleic acids and small molecules.
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Cat. # Quantity Price Lead time
61150 55 mL $49.00 in stock

Copper(II)-TBTA complex

A catalyst for click reaction. A stable copper(II) complex that must be converted to monovalent copper(I) in situ using reducing agents.
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Cat. # Quantity Price Lead time
21050 1.5 mL $55.00 in stock

DMSO, labeling grade

Pure dimethyl sulfoxide (DMSO) solvent with quality optimized for bioconjugation reactions.
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Cat. # Quantity Price Lead time
15050 1 mL $10.00 in stock

EdU (5-Ethynyl-2'-deoxyuridine)

EdU is a nucleoside that is incorporated into replicated DNA by cellular enzymes. After it, DNA contained in the cells can be developed by click chemistry reaction with fluorescent dye azides to reveal cell proliferation.
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Cat. # Quantity Price Lead time
10540 100 mg $120.00 in stock
20540 250 mg $195.00 in stock
30540 500 mg $345.00 in stock
40540 1 g $590.00 in stock
60540 5 g please inquire 21 days

F-ara-EdU (2’-Deoxy-2’-fluoro-5-ethynyluridine)

F-ara-EdU is a synthetic analog of thymidine used to study de novo DNA synthesis and cell proliferation. It is a less cytotoxic alternative for BrdU and EdU.
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Cat. # Quantity Price Lead time
3438-25mg 25 mg $290.00 in stock
3438-100mg 100 mg $640.00 in stock
3438-500mg 500 mg please inquire in stock

HPG (L-Homopropargylglycine)

Noncanonical amino acid analog of methionine with a terminal alkyne moiety for nascent protein synthesis detection.
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Cat. # Quantity Price Lead time
3313-25mg 25 mg $129.00 21 days
3313-100mg 100 mg $249.00 21 days
3313-1g 1 g please inquire 21 days

Protein labeling buffer (with THPTA), 1.5x

Catalytic buffer for click chemical modification of proteins.
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Cat. # Quantity Price Lead time
B5150 1 mL $30.00 in stock
K5150 10 mL $120.00 in stock
S5150 50 mL $490.00 in stock

THPTA ligand

THPTA is a water-soluble ligand for Cu(I) catalyzed click chemistry. The ligand stabilizes copper in its Cu(I) oxidation state. Due to high aqueous solubility, reactions with this ligand do not require an organic co-solvent.
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Cat. # Quantity Price Lead time
F4050 100 mg $40.00 in stock
H4050 500 mg $199.00 in stock
I4050 1 g $369.00 in stock
K4050 5 g $1568.00 in stock
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