Nucleic acid reagents

Nucleic acid reagents / product groups & items in this category
Reagents for nucleic acid modification, isolation, and quantification. Reagents for PCR/qPCR.

Nucleic acid quantification

Kits for nucleic acid quantification compatible with various fluorescence measuring equipment.
    • PreciseGreen® dsDNA Quantification Kit
      A kit for the quantification of dsDNA using PreciseGreen® fluorescent dye.
    • QuDye® dsDNA HS Assay Kit
      Ready kit optimised for fluorometers featuring high sensitivity (HS) and a measurements range from 10 pg/uL to 100 ng/uL.
    • QuDye® ssDNA Assay Kit
      Ready kit for accurate and sensitive quantification of single-stranded DNA with fluorometer; the measurement range is from 50 pg/μL to 200 ng/μL for initial ssDNA sample.
    • QuDye® dsDNA BR Assay Kit
      Ready kit for accurate DNA quantification with fluorometer in the broad range from 100 pg/µL to 1000 ng/µL. Common contaminants are well tolerated in the assay, only 1-20 µL of experimental sample is required. Measurements are performed at room temperature.
    • Ribo488 RNA Plate Reader Assay Kit
      Ribo488 fluorescent dye kit for quantitative measurement of RNA concentration in solution. It is compatible with cuvette and microvolume fluorometers. Kit includes buffer and RNA standard.
    • Ribo488 RNA BR Fluorimetric Assay Kit
      RNA quantification kit designed to measure a broad range of RNA concentrations on any fluorimeter.
    • QuDye® dsDNA HS Rapid Kit
      QuDye® dsDNA HS Rapid Kit is designed for rapid, highly sensitive quantification of double-stranded DNA using a fluorescence-based method on a fluorometer.
    • PreciseGreen® dsDNA Quantification Reagent, 200×
      PreciseGreen® is a dye for precise fluorescence-based quantification of dsDNA in the presence of RNA, ssDNA, and other impurities. The dye exhibits bright fluorescence in the fluorescein channel, which provides a linear response to DNA concentration within the range of low pg/mL to high ng/mL.
    • QuDye® dsDNA HS Reagent, 200×
      Concentrated reagent for high sensitive (HS) double-stranded (dsDNA) quantification to be used in any fluorescence reader.
    • QuDye® dsDNA BR Reagent, 200×
      Concentrated reagent for quantitation of double-stranded DNA in a broad range initial sample concentration using a fluorescence plate reader or fluorometer.
    • Ribo488 RNA Quantification Reagent
      Ribo488 is the fluorescent intercalating dye for quantitation of RNA.
    • QuReader® 1 Green
      Green channel single sample model fluorometer optimized for QuDye® quantification kits.
    • QuReader® 8 Green
      Green channel multiple samples model fluorometer optimized for QuDye® quantification kits.

Nucleic acid isolation

Ready-to-use kits for sample preparation and isolation of nucleic acids from various biological samples and agarose gels.

PCR/qPCR

Nucleic acid stains, intercalating dyes, DNA-binding dyes, reference dyes for PCR, and other fluorescent tools for DNA amplification and detection.
    • DryDrops® PCR UDG, Single Assay PCR Lyophilized Beads
      Pre-formulated, pre-dispensed, single-dose lyophilized reaction mixture with uracil-DNA glycosylase (UDG) and all the necessary components for carrying out PCR with a volume of 25 μL.
    • DryDrops® PCR, Single Assay PCR Lyophilized Beads
      Pre-formulated, pre-dispensed, single-dose lyophilized reaction mixture with all the necessary components for carrying out PCR and real-time PCR with a volume of 25 μL.
    • ProbeMaster® Lyo UNI, 5×
      A ready-to-use lyophilized reaction mixture containing all the necessary components for both traditional PCR (with end-point analysis) and quantitative PCR.
    • ProbeMaster® Lyo ROX, 5×
      Lyophilized ready-to-use reaction mixture for quantitative PCR (qPCR), containing the ROX reference dye.
    • ProbeMaster® Lyo UDG, 5×
      A lyophilized, ready-to-use reaction mixture containing UDG and dUTP for quantitative PCR.
    • ProbeMaster® Lyo GEL UDG, 5×
      Lyophilized reaction mixture for PCR with subsequent analysis by gel electrophoresis. The mixture contains UDG and dyes for application to the gel and monitoring the electrophoresis time.
    • ProbeMaster® Lyo GEL, 5×
      A lyophilized, ready-to-use reaction mixture for PCR followed by gel electrophoresis analysis. The mixture contains dyes for gel application and time control during electrophoresis.
    • dsGreen®, solid form
      dsGreen® is a stain with high sensitivity and selectivity for dsDNA. This product is a solid form of dsGreen that can be used for the preparation of solutions.
    • dsGreen® for Real-Time PCR, 100×
      Fluorescent dye for detection of amplification in real-time PCR in a ready-to-use 100× stock solution for adding to the master mix.
    • dsGreen® Lyophilized qPCR kit
      Lyophilized, ready-to-use, high performance qPCR kit with hot start Taq polymerase and dsGreen fluorescent dye. All of the active components are freeze-dried allowing to be shipped at room temperature.
    • ROX reference dye for qPCR
      ROX is a passive reference dye for qPCR amplification. The dye can be used to normalize fluorescence intensity of reporter dye in qPCR.
    • Random(dN)6 primer
      Random-sequence oligonucleotide can be used as a universal primer.
    • Random(dN)10 primer
      Random-sequence oligonucleotide can be used as a universal primer.
    • ProbeMaster® Lyo dsGreen, 5×
      A ready-to-use lyophilized reaction mixture containing the intercalating dye dsGreen for conventional PCR (with endpoint analysis) and quantitative PCR.

Nucleic acid gel electrophoresis

Highly sensitive dyes for staining RNA, single-stranded DNA (ssDNA), or double-stranded DNA (dsDNA) in agarose and polyacrylamide electrophoretic gels and other reagents for electrophoresis.
Group tags PCR and qPCR

Modified triphosphates

Triphosphates for enzymatic labeling and modification of DNA with fluorescent dyes, reactive groups, and affinity groups.
    • AF 488 dUTP
      AF 488 dUTP is a fluorescently modified deoxynucleotide triphosphate for synthesizing labeled DNA probes.
    • Amino-11-CTP
      Amino-11-CTP is a modified cytidine triphosphate for amine labeling of RNA.
    • Amino-11-dCTP
      Amino-11-dCTP is a modified deoxycytidine triphosphate for amine labeling of DNA.
    • Amino-11-UTP
      Uridine triphosphate labeled with amino group intended for indirect non-radioactive enzymatic RNA labeling.
    • Amino-11-dUTP
      Amino-11-dUTP is a triphosphate for the modification of DNA.
    • Biotin-11-CTP
      Non-fluorescent modified nucleotide for preparing biotin-labeled of RNA in purification and detection applications.
    • Biotin-11-dCTP
      Biotinylated dCTP with 11-atom linker for non-radioactive DNA-labeling.
    • Biotin-11-UTP
      Biotin-11-UTP is a substrate for RNA-polymerases SP6, T3, and T7. Biotinylated triphosphate is an analog of natural UTP and can be used for RNA labeling in transcription in vitro.
    • Biotin-11-dUTP
      Biotin-labeled deoxyuridine triphosphate for enzymatic incorporation into DNA and indirect detection.
    • FAM-11-dCTP, 6-isomer
      6-isomer FAM-labeled dCTP with 11 carbon atoms linker for enzymatic DNA labeling.
    • FAM-11-UTP, 6-isomer
      6-FAM-uridine triphosphate for non-radioactive enzymatic RNA labeling during in vitro transcription.
    • FAM-11-dUTP, 6-isomer
      FAM-derivative of dUTP with green emission for DNA labeling.
    • sulfo-Cyanine3 dUTP
      Fluorescent dNTP for the labeling of DNA with sulfo-Cyanine3.
    • sulfo-Cyanine5 dUTP
      sulfo-Cyanine5 dUTP is a fluorescently labeled triphosphate used to enzymatically modify DNA with a sulfo-Cyanine5 fluorophore. The reagent contains a long linker arm, which makes incorporation highly efficient.
    • sulfo-Cyanine5.5 dUTP
      Modified deoxy triphosphate containing sulfo-Cyanine5.5 (aka Cy5.5) dye for enzymatic labeling of DNA microarrays.
    • Amino-11-ddUTP
      Amino-11-ddUTP is a triphosphate for the modification of DNA.
    • Biotin-11-ddUTP
      Biotinylated dideoxyuridine triphosphate analog for the enzymatic preparation of biotin-labeled oligonucleotide probes, end-labeling of DNA fragments, DNA sequencing, SNP detection, and probe labeling.
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