FAM-11-UTP, 6-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2714-50nmol | 50 nmol | $115 | in stock | |
| 2714-250nmol | 250 nmol | $325 | in stock | |
| 2714-500nmol | 500 nmol |
$500
|
in stock | |
| 2714-1umol | 1 umol |
$910
|
in stock |
6-fluorescein (FAM) derivative of uridine triphosphate (UTP). FAM is a fluorophore with a high quantum yield. The maximum emission of the fluorescence is at a wavelength of 513 nm in the green spectrum range.
6-FAM-11-UTP can be used as a substrate for RNA-polymerases T7, T3 and SP6 during in vitro transcription. RNA-probes produced with this method can be used for fluorescence hybridization in situ, including multiplex, and for Northern blot. Fluorescently-labeled cRNA can be used for microarray-based gene expression profiling.
Absorption and emission spectra of FAM
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| Appearance: | yellow/orange solid |
| Molecular weight: | 1026.54 |
| Molecular formula: | C39H36Li3N4O22P3 |
| IUPAC name: | ((2R,3S,4R,5R)-5-(5-(3-(6-(3-carboxy-4-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzamido)hexanamido)prop-1-yn-1-yl)-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl hydrogen triphosphate |
| Solubility: | soluble in water |
| Quality control: | HPLC-MS (95%), testing in enzymatic reaction |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light and excessive freeze-thaw cycles. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 492 |
| ε, L⋅mol−1⋅cm−1: | 74000 |
| Emission maximum, nm: | 517 |
| Fluorescence quantum yield: | 0.93 |
| CF260: | 0.22 |
| CF280: | 0.17 |
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