Protein labeling calculator
Dissolve label_stock_weight_mg mg of the label in label_stock_vol_ul of DMSO.
Take label_stock_vol_per_rxn_ul of label stock solution into a separate vial.
Dilute with extra_dmso_vol_ul of DMSO.
Take label_stock_weight_mg mg of the label.
Dissolve protein (protein_weight_mg mg) in water_vol_per_rxn_ul of water, add NaHCO3_stock_vol_ul of NAHCO3_stock_concentration_moll.
Take prot_vol_per_rxn_ul of protein solution, add water_vol_per_rxn_ul of water and NaHCO3_stock_vol_ul of NAHCO3_stock_concentration_moll solution, mix thoroughly.
Add protein solution to the solid label, mix thoroughly, and incubate. Purify by dialysis, or by gel filtration.
Add label solution to protein solution, mix thoroughly, and incubate. Purify by dialysis, or by gel filtration.
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