Fluorophores

Lumiprobe manufactures and offers a range of fluorophores of different classes for various applications. Currently available fluorophores are summarized on this page. Click on a fluorophore name to read its description and see a list of the products containing it.

Please also check our fluorophore chart summarizing dye excitation and emission wavelengths.

Cyanine3

Cyanine3 is a cyanine fluorophore with yellow emission. It has a very high extinction coefficient that makes it bright. Often used for microscopy, Cy3 channel is available on many instruments.

Cyanine3B

Cyanine3B is a yellow-emitted cyanine dye that is an improved version of Cyanine3 fluorophore with significantly increased fluorescence quantum yield and photostability.

Cyanine3.5

Excitation and emission wavelengths of Cyanine3.5 are intermediate between those for Cyanine3, and Cyanine5.

Cyanine5

Cyanine5 is one of the most popular fluorophores for different applications, compatible with a number of various fluorescent instruments. It is used for microscopy including STORM, for qPCR, in FRET experiments with Cyanine3 donor, and for many other applications.

Cyanine5.5

Far red / near infrared dye for applications requiring low background. Suitable for in vivo imaging applications.

Cyanine7

Near infrared cyanine fluorophore with a rigidized structure for improved quantum yield - a good choice for in vivo imaging.

Cyanine7.5

Near infrared fluorophore useful for non-invasive in vivo imaging. Absorption properties are similar to indocyanine green (ICG), but possesses higher fluorescence quantum yield.

sulfo-Cyanine3

Water soluble, sulfonated version of Cyanine3, universal fluorophore with excellent photophysical properties, an equivalent of Cy3® dye.

sulfo-Cyanine3.5

Water-soluble dye with emission in the yellow-orange spectrum range with a maximum at 602 nm.

sulfo-Cyanine5

Sulfonated, water soluble version of Cyanine5 fluorophore, equivalent of Cy5®, a universal dye. Very bright dye with red emission. Most fluorescent instruments with filters have an appropriate channel for this dye.

sulfo-Cyanine5.5

Sulfo-Cyanine5.5 is a sulfonated far red emitting fluorophore which is highly water-soluble and hydrophilic.

sulfo-Cyanine7

Near infrared water soluble cyanine fluorophore, mainly useful for non-invasive in vivo imaging.

sulfo-Cyanine7.5

Sulfo-Cyanine7.5 is a water soluble near infrared fluorophore. Its absorption spectrum, structure, and hydrophilicity resemble indocyanine green (ICG), but it has a significantly higher fluorescence quantum yield. This dye is useful for non-invasive in vivo NIR imaging.

AF430

AF430 is a hydrophilic, sulfonated coumarin dye with emission in green-yellow range.

AF488

AF488 is a bright, photostable, and hydrophilic fluorophore for green (FAM) channel.

AF594

AF594 is a bright water-soluble dye that is not sensitive to pH changes within the range from 4 to 10. This red-fluorescent dye is commonly used for flow cytometry and fluorescent microscopy.

AF568

AF568 is a fluorescent water-soluble dye with excellent photochemical stability and brightness.

AF647

AF647 is a bright, photostable, and hydrophilic fluorophore emitting in the far-red channel.

BDP FL

Borondipyrromethene dye for fluorescein (488 nm) channel - very bright and photostable fluorophore.

BDP R6G

BDP R6G is a borondipyrromethene dye which is specially tuned to match excitation and emission channels of the classical rhodamine 6G dye. BDP R6G possesses a high extinction coefficient, and excellent quantum yield. The fluorophore is ideal for various labeling applications.

BDP TMR

Borondipyrromethene fluorophore with absorption and emission spectra similar to TAMRA, but much brighter, and very photostable.

BDP 558/568

BDP 558/568 is a fluorescent dye with emission in the yellow region of the spectrum.

BDP 564/570

Hydrophobic dye from BDP family with emission in the yellow spectrum range, a BODIPY 564/570 analog. It has high quantum yield and high molar extinction coefficient and can be used for fluorescent microscopy.

BDP 576/589

Hydrophobic boron dipyrromethene dye emitting in the orange spectrum range. High quantum yield and molar extinction coefficient. Long excited-state lifetime and large two-photon absorption cross-section.

BDP 581/591

BDP 581/591 is a relatively hydrophobic borondipyrromethene fluorophore that can be used to probe reactive oxygen species (ROS), or as a fluorescence polarization tracer. It features high brightness, a significant two photon cross-section, and a relatively long fluorescence lifetime.

BDP TR

BDP TR is a borondipyrromethene dye for ROX channel.

BDP 630/650

BDP 630/650 is a bright and photostable far red fluorophore. It possesses narrow absorption and emission peaks as well as high extinction coefficient and quantum yield. Its relatively long excited state lifetime and significant two photon cross section makes it ideal for two photon experiments, and for fluorescence polarization assays.

BDP 650/665

BDP 650/665 is a far red emitting fluorophore, compatible with Cy5 channel.

FAM (Fluorescein)

Traditional fluorescein (FAM) fluorophore - universal dye useful for various applications, compatible with most of existing fluorescent instruments. All products are available as pure 5- or 6-isomers.

R6G

Rhodamine 6G is a traditional dye that has a long history of use in qPCR and other applications, mainly in the nucleic acid field.

TET (Tetrachlorofluorescein)

TET or tetrachlorofluorescein is a fluorescein derivative with green emission.

HEX (Hexachlorofluorescein)

HEX is a green-yellow emitting dye; the HEX channel is one of the main channels in Multiplex qPCR.

SIMA (Dichloro-diphenyl-fluorescein)

SIMA or dichloro-diphenyl-fluorescein is a dye with spectral properties similar to HEX, but with a higher quantum yield. SIMA also has improved stability during deprotection under basic conditions.

VIC

VIC is an asymmetrical xanthene dye with yellow-green fluorescence.

JOE

JOE is a xanthene fluorophore with yellow fluorescence.

TAMRA (TMR, tetramethylrhodamine)

Tetramethylrhodamine (TMR, TAMRA) fluorophore is a traditional rhodamine dye which forms a FRET pair with fluorescein.

ROX (Rhodamine X, Rhodamine 101)

ROX (rhodamine X or rhodamine 101) is a bright rhodamine dye that has found particularly wide use in qPCR.

Rh123 (Rhodamine 123)

Rh123 (Rhodamin 123) is a cell-permeant, green-fluorescent xanthene dye аccumulated in membranes in a potential-dependent manner.

Coumarin 343

Coumarin 343 is a blue emitting fluorophore that forms a FRET pair with FAM as a donor.

Pyrene

Pyrene is a fluorescent polycyclic aromatic hydrocarbon. It is useful as a proximity sensor - when two pyrene residues are spaced close to each other, they exhibit easily detectable excimer fluorescence.

PEP (Phenylethynyl-pyrene)

Phenylethynylpyrene (PEP) is a pyrene derivative with red-shifted absorption and emission spectra. Fits blue channel of qPCR instruments, can be used for multiplex experiments.

Perylene

Bright and extremely photostable polycyclic aromatic hydrocarbon fluorophore with the quantum yield approaching unity, especially useful for materials science, and supramolecular constructs.

DusQ 21

DusQ 21 is a fluorescence quencher in far red and near infrared region.
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