AF430 in Fluorophores

Absorption max 430 nm Emission max 542 nm Fluorophore chart

AF430 is a coumarin dye identical to Alexa Fluor™ 430 with absorption maximum at 434 nm and emission in green-yellow range (maximum at 541 nm). The dye is one of few fluorophores that absorb in the range between UV-excited fluorophores (e.g. DAPI with an excitation peak at 358 nm, Alexa Fluor 350 at 346 nm, AMCA/AMCA-X at ~350 nm) and fluorescein (maximum at 494 nm). AF430 is a hydrophilic dye — sulfo group provides better hydrophilicity. The dye is photostable — trifluoromethyl group in its structure reduces photobleaching while sulfo group provides higher quantum yield in aqueous solution. Its fluorescence is independent of pH over a wide range of pH values (from pH 4 to pH 10) and can be excited by 405 nm violet laser.

AF430 possesses a large Stokes shift of 112 nm which makes the dye valuable for multi-color imaging applications, including wide-field and confocal fluorescence microscopy, STED microscopy, and flow cytometry. Fluorophores with large Stokes shift allow reducing cross-talk and the number of excitation/detection channels.

As the dye exhibits high photostability, it is suitable for STED microscopy where enormous light intensities are used. AF430 is applied in multicolor STED microscopy by combining two dyes — one with a large and one with a small Stokes shift. Depletion of both dyes can be performed with one wavelength (AF430 can be depleted by 660 nm laser).

Due to its specific spectral properties, AF430 is often used in FRET experiments both in material and life sciences.

AF430 alkyne

AF430 is a dye emitting in green-yellow range. A dye is useful for flow cytometry.
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Cat. # Quantity Price Lead time
A08B0 1 mg $110.00 in stock
B08B0 5 mg $290.00 in stock
C08B0 10 mg $470.00 in stock
D08B0 25 mg $690.00 in stock
E08B0 50 mg $1270.00 in stock
F08B0 100 mg $1990.00 in stock

AF430 azide

Azide derivative of AF430 for labeling and detection of alkyne-containing biomolecules via Click chemistry reaction
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Cat. # Quantity Price Lead time
A0830 1 mg $110.00 in stock
B0830 5 mg $290.00 in stock
D0830 25 mg $690.00 in stock
E0830 50 mg $1270.00 in stock
F0830 100 mg $1990.00 in stock

AF430 carboxylic acid

Non-reactive form of AF430 fluorescent dye to be used as a reference standard
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Cat. # Quantity Price Lead time
10890 1 mg $110.00 21 days
20890 5 mg $165.00 21 days
40890 25 mg $380.00 21 days
50890 50 mg $695.00 21 days
60890 100 mg $1190.00 21 days

AF430 maleimide

AF430 is a green-yellow emitting fluorescent dye of coumarin series. This maleimide derivative allows to label thiolated biomolecules.
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Cat. # Quantity Price Lead time
10880 1 mg $110.00 in stock
20880 5 mg $290.00 in stock
40880 25 mg $690.00 in stock
50880 50 mg $1270.00 in stock
60880 100 mg $1990.00 in stock

AF430 NHS ester

AF430 is a hydrophilic coumarin dye with the emission in green-yellow range. The NHS ester is used for the labeling of amine groups.
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Cat. # Quantity Price Lead time
10820 1 mg $110.00 in stock
20820 5 mg $165.00 in stock
40820 25 mg $380.00 in stock
50820 50 mg $695.00 in stock
60820 100 mg $1190.00 in stock

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