AF 430 alkyne

AF 430 Alkynes Click chemistry

Cat. #	Quantity	Price	Lead time
A08B0	1 mg	$125	in stock
B08B0	5 mg	$325	in stock
C08B0	10 mg	$480	in stock
D08B0	25 mg	$950	in stock
E08B0	50 mg	$1625	in stock
F08B0	100 mg	$2090	in stock

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AF 430 is a sulfonated coumarin dye with emission in the yellow-green region of the spectrum that is useful for flow cytometry and antibody labeling. The fluorescence is constant in wide range of pH values.

This particular derivative contains a terminal alkyne group that is suitable for copper catalyzed CuAAС reaction.

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Absorption and emission spectra of AF 430

Recommended protocol

Conjugation of Alkyne- or Azide-modified Proteins with Dye Azides or Alkynes

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General properties

Appearance:	orange solid
Molecular weight:	578.64
Molecular formula:	C₂₅H₂₆N₂F₃KO₆S
Solubility:	good in water, DMF, DMSO
Quality control:	NMR ¹H, HPLC-MS (95%)
Storage conditions:	Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	430
ε, L⋅mol⁻¹⋅cm⁻¹:	15955
Emission maximum, nm:	542
Fluorescence quantum yield:	0.23

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.