AF488 DBCO, 5-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1118F0 | 1 mg | $110.00 | 21 days | |
| 2118F0 | 5 mg | $290.00 | 21 days | |
| 4118F0 | 25 mg |
$690.00
|
21 days | |
| 5118F0 | 50 mg |
$1270.00
|
21 days | |
| 6118F0 | 100 mg |
$1990.00
|
21 days |
Dibenzocyclooctyne (DBCO, DBCO, ADIBO) is one of the most reactive cycloalkynes for copper-free click reaction (SPAAC, strain-promoted azide-alkyne cycloaddition). The rate of interaction of DBCO with azides is significantly higher than that of other cyclooctynes, as well as Cu-catalyzed click reaction (CuAAC). Unlike other cyclooctynes, DBCO does not interact with tetrazines, which makes it possible to use it in bioorthogonal reactions together with trans-cyclooctenes and tetrazines.
AF488 is sulfonated rhodamine, a bright, photostable, and hydrophilic fluorophore that emits in the green channel. The absorption maximum is 495 nm. The emission maximum is 519 nm.
AF488 DBCO allows fluorescent labeling of azide-containing biomolecules inside living cells, whole organisms, and inanimate samples.
Absorption and emission spectra of AF488
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| Molecular weight: | 936.08 |
| Molecular formula: | C48H49N5O11S2 |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 495 |
| ε, L⋅mol−1⋅cm−1: | 71800 |
| Emission maximum, nm: | 519 |
| Fluorescence quantum yield: | 0.91 |
| CF260: | 0.16 |
| CF280: | 0.10 |
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