AF 488 DBCO

Cycloalkynes AF 488 Copper-free click chemistry
Cat. # Quantity Price Lead time
118F0 1 mg $140 5 days
218F0 5 mg $450 5 days
418F0 25 mg $1460 in stock
518F0 50 mg $1890 5 days
618F0 100 mg $2640 in stock
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Dibenzocyclooctyne (DBCO, DBCO, ADIBO) is one of the most reactive cycloalkynes for copper-free click reaction (SPAAC, strain-promoted azide-alkyne cycloaddition). The rate of interaction of DBCO with azides is significantly higher than that of other cyclooctynes, as well as Cu-catalyzed click reaction (CuAAC). Unlike other cyclooctynes, DBCO does not interact with tetrazines, which makes it possible to use it in bioorthogonal reactions together with trans-cyclooctenes and tetrazines.

AF 488 is sulfonated rhodamine, a bright, photostable, and hydrophilic fluorophore that emits in the green channel. The absorption maximum is 495 nm. The emission maximum is 519 nm.

AF 488 DBCO allows fluorescent labeling of azide-containing biomolecules inside living cells, whole organisms, and inanimate samples.

AF 488 DBCO, 5-isomer, 418F0
AF 488 DBCO, 5-isomer, 418F0
AF 488 DBCO, 5-isomer, 418F0
Structure of AF 488 DBCO, 5-isomer
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Absorption and emission spectra of AF 488

Absorption and emission spectra of AF 488

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General properties

Appearance: orange solid
Molecular weight: 936.08
Molecular formula: C48H49N5O11S2
Solubility: water, DMSO, DMF, methanol
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 495
Emission maximum, nm: 519
Fluorescence quantum yield: 0.91

Product citations

  1. Xu, W.; He, M.; Lu, Q. Fibronectin Connecting Cell Sheet Based on Click Chemistry for Wound Repair. Advanced Science, 2024, 11(11), 2306746. doi: 10.1002/advs.202306746
  2. Xia, Q.; Perera, H. A.; Bolarinho, R.; Piskulich, Z. A.; Guo, Z.; Yin, J.; He, H.; Li, M.; Ge, X.; Cui, Q.; Ramström, O.; Yan, M.; Cheng, J.-X. Click-Free Imaging of Carbohydrate Trafficking in Live Cells Using an Azido Photothermal Probe. Science Advances, 2024, 10(34), eadq0294. doi: 10.1126/sciadv.adq0294
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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