AF488 DBCO, 5-isomer

Cat. # Quantity Price Lead time
1118F0 1 mg $110.00 21 days
2118F0 5 mg $290.00 21 days
4118F0 25 mg $690.00 21 days
5118F0 50 mg $1270.00 21 days
6118F0 100 mg $1990.00 21 days

Dibenzocyclooctyne (DBCO, DBCO, ADIBO) is one of the most reactive cycloalkynes for copper-free click reaction (SPAAC, strain-promoted azide-alkyne cycloaddition). The rate of interaction of DBCO with azides is significantly higher than that of other cyclooctynes, as well as Cu-catalyzed click reaction (CuAAC). Unlike other cyclooctynes, DBCO does not interact with tetrazines, which makes it possible to use it in bioorthogonal reactions together with trans-cyclooctenes and tetrazines.

AF488 is sulfonated rhodamine, a bright, photostable, and hydrophilic fluorophore that emits in the green channel. The absorption maximum is 495 nm. The emission maximum is 519 nm.

AF488 DBCO allows fluorescent labeling of azide-containing biomolecules inside living cells, whole organisms, and inanimate samples.

Absorption and emission spectra of AF488

Absorption and emission spectra of AF488

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General properties

Molecular weight: 936.08
Molecular formula: C48H49N5O11S2
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 495
ε, L⋅mol−1⋅cm−1: 71800
Emission maximum, nm: 519
Fluorescence quantum yield: 0.91
CF260: 0.16
CF280: 0.10
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