AF 488 DBCO

Cat. # Quantity Price Lead time
118F0 1 mg $140 in stock
218F0 5 mg $450 in stock
418F0 25 mg $1460 in stock
518F0 50 mg $1890 in stock
618F0 100 mg $2640 in stock
Found better price? Let us know and we will propose the way forward!

Dibenzocyclooctyne (DBCO, DBCO, ADIBO) is one of the most reactive cycloalkynes for copper-free click reaction (SPAAC, strain-promoted azide-alkyne cycloaddition). The rate of interaction of DBCO with azides is significantly higher than that of other cyclooctynes, as well as Cu-catalyzed click reaction (CuAAC). Unlike other cyclooctynes, DBCO does not interact with tetrazines, which makes it possible to use it in bioorthogonal reactions together with trans-cyclooctenes and tetrazines.

AF 488 is sulfonated rhodamine, a bright, photostable, and hydrophilic fluorophore that emits in the green channel. The absorption maximum is 495 nm. The emission maximum is 519 nm.

AF 488 DBCO allows fluorescent labeling of azide-containing biomolecules inside living cells, whole organisms, and inanimate samples.

Absorption and emission spectra of AF 488

Absorption and emission spectra of AF 488

Customers also purchased with this product

AF 594 azide

Azide derivative of water-soluble red-fluorescent dye AF 594 for labeling alkyne-containing biomolecules and fluorescent visualization of biological objects. Pure 6-isomer.

AF 594 NHS ester

Red-fluorescent NHS-ester of AF 594 dye for labeling proteins, peptides, antibodies, and any molecules containing an -NH2 group. Pure 5-isomer
Add this product to your cart and
get free express delivery

AF 555 NHS ester

AF 555 NHS ester, a bright, photostable, and hydrophilic fluorescent dye for the yellow channel.

General properties

Appearance: orange solid
Molecular weight: 936.08
Molecular formula: C48H49N5O11S2
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 495
Emission maximum, nm: 519
Fluorescence quantum yield: 0.91

Product citations

  1. Xia, Q.; Perera, H.A.; Bolarinho, R.; Piskulich, Z.A.; Guo, Z.; Yin, J.; He, H.; Li, M.; Ge, X.; Cui, Q.; Ramström, O.; Yan, M.; Cheng, J.-X. Click-free imaging of carbohydrate trafficking in live cells using an azido photothermal probe. bioRxiv, 2024, preprint. doi: 10.1101/2024.03.08.584185
  2. Xu, W.; He, M.; Lu, Q. Fibronectin Connecting Cell Sheet Based on Click Chemistry for Wound Repair. Advanced Science, 2024, 11(11), 2306746. doi: 10.1002/advs.202306746
Your item has been added. View your cart or proceed to checkout
The count of items is incorrect.