Azidobutyric acid NHS ester

NHS esters Cross linking reagents Protein labeling
Cat. # Quantity Price Lead time
53720 50 mg $60.00 in stock
63720 100 mg $95.00 5 days
73720 1 g please inquire in stock
83720 5 g please inquire in stock
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Convert your proteins and peptides into click chemistry reactive form with this reagent.

While click chemistry involves reaction between terminal alkyne and azide, both azides and alkynes are very uncommon in nature. However, there are reagents to attach these fragments to abundant amino groups which are ubiquitous in the world of biomolecules. This azido-NHS ester is designed for the conversion of proteins, peptides, amino-DNA, and other amines into click chemistry reactive azides.

Azidobutyric acid NHS ester
Azidobutyric acid NHS ester
Azidobutyric acid NHS ester
Structure of azidobutyric acid NHS ester
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DBCO NHS ester

Azodibenzocyclooctyne (DBCO, ADIBO) reagent for strain promoted copper-free click chemistry (SPAAC). The reagent contains an NHS ester function for the attachment of the cyclooctyne to various molecules.

General properties

Appearance: colorless solid
Mass spec M+ increment: 111.0
Molecular weight: 226.19
CAS number: 943858-70-6
Molecular formula: C8H10N4O4
IUPAC name: Butanoic acid, 4-​azido-​, 2,​5-​dioxo-​1-​pyrrolidinyl ester
Solubility: soluble in organic solvents (DMF, DMSO)
Quality control: NMR 1H (95%), HPLC
Storage conditions: Storage: 12 months after receival at -20°C. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Product citations

  1. Wang, Y.; Yao, J.; Cai, L.; Liu, T.; Wang, X.; Zhang, Y.; Zhou, Z.; Li, T.; Liu, M.; Lai, R.; Liu, X. Bone-targeted extracellular vesicles from mesenchymal stem cells for osteoporosis therapy. Research Square, preprint. doi: 10.21203/rs.3.rs-18279/v1
  2. Kleinpenning, F.; Steigenberger, B.; Wu, W.; Heck, A.J.R. Fishing for newly synthesized proteins with phosphonate-handles. Nature Communications, 2020, 11, 3244. doi: 10.1038/s41467-020-17010-0
  3. Blanchard, A.T.; Bazrafshan, A.S.; Yi, J.; Eisman, J.T.; Yehl, K.M.; Bian, T.; Mugler, A.; Salaita, K. Highly Polyvalent DNA Motors Generate 100+ pN of Force via Autochemophoresis. Nano Letters, 2019, 19(10), 6977–6986. doi: 10.1021/acs.nanolett.9b02311
  4. Hou, W.; Li, Y.; Kang, W.; Wang, X.; Wu, X.; Wang, S.; Liu, F. Real-time analysis of quantum dot labeled single porcine epidemic diarrhea virus moving along the microtubules using single particle tracking. Scientific Reports, 2019, 9, 1307. doi: 10.1038/s41598-018-37789-9
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