Copper-free click chemistry reactions include cycloadditions for bioconjugation that do not rely on the use of transition metal catalyst, as opposed to traditional copper catalyzed сlick chemistry.
These reactions include the strain promoted alkyne-azide cycloaddition (SPAAC) that involves strained cycloalkyne, and azide. Relief of the sterical strain provides a driving force for this reaction.
DBCO (ADIBO) cycloalkynes are among the most reactive yet stable. The reaction yields two regioisomers that usually cannot be separated, therefore this does not lead to peak doubling in HPLC.
Another process is TCO ligation (tetrazine - trans- cyclooctene ligation) which is also powered by sterical strain. Unlike SPAAC, this is [4+2]-cycloaddition yielding 6-membered ring. TCO ligation is among the fastest reactions used for the bioconjugation.
Both these reactions are very biocompatible as they do not require to use a metal catalyst, they are fast, and orthogonal in case of DBCO cycloalkynes (i.e., it is possible to run two reaction in one mixture without interference).
Lumiprobe manufactures a number of reagents for copper-free click-chemistry, for both SPAAC, and TCO ligation.
Unnatural L-phenylalanine derivative with an azide moiety for nascent protein synthesis detection via copper-catalyzed and copper-free click chemistry.
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3415-100mg
100 mg
$125.00
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3415-500mg
500 mg
$625.00
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An azide-functionalized monosaccharide for metabolic labeling and studying the cellular trafficking of labeled proteins and proteomic analysis.
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3706-5mg
5 mg
$129.00
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3706-25mg
25 mg
$240.00
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3706-100mg
100 mg
$630.00
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An azide-functionalized monosaccharide for metabolic labeling and studying the cellular trafficking of labeled proteins and proteomic analysis.
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3662-5mg
5 mg
$129.00
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3662-25mg
25 mg
$240.00
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3662-100mg
100 mg
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An azide-functionalized monosaccharide for metabolic labeling and studying the cellular trafficking of labeled proteins and proteomic analysis.
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3159-5mg
5 mg
$129.00
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3159-25mg
25 mg
$240.00
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3159-100mg
100 mg
$630.00
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Tetrazine derivative of AF 430 fluorescent dye suitable for in vitro cell labeling.
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Dibenzocyclooctyne (DBCO, ADIBO) containing the green fluorophore AF 488 for labeling azide-tagged biomolecules inside live cells, whole organisms, and inanimate samples. Pure 5-isomer.
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118F0
1 mg
$140.00
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218F0
5 mg
$450.00
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418F0
25 mg
$1460.00
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518F0
50 mg
$1890.00
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618F0
100 mg
$2640.00
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Dibenzocyclooctyne (DBCO, ADIBO) containing the orange fluorophore AF 568 for cooper-free labeling azide-tagged biomolecules inside live cells, whole organisms, and inanimate samples.
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1158F0
1 mg
$140.00
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2158F0
5 mg
$450.00
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4158F0
25 mg
$1460.00
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5158F0
50 mg
$1890.00
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6158F0
100 mg
$2990.00
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Noncanonical amino acid analog of methionine with an azide moiety for nascent protein synthesis detection via copper-catalyzed and copper-free click chemistry.
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3799-25mg
25 mg
$125.00
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3799-100mg
100 mg
$235.00
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Azidomethyldeoxyuridine (AmdU) is an azide-contained nucleoside that is incorporated into nascent DNA by cellular polymerases. The azide groups attached to DNA this way can be subsequently modified with alkynes or cycloalkynes via copper-catalyzed or strain-promoted click reaction.
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B1540
5 mg
$120.00
in stock
D1540
25 mg
$255.00
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F1540
100 mg
$890.00
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A hydrophilic bifunctional linker with azide and tetrazine function groups flanking PEG3 (triethylene glycol) spacer.
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2798-250mg
250 mg
$549.00
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BCN-PEG4-Cyanine3 is an ethylene glycol separated linker for the azido-modified molecules for copper-free click conjugation.
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3269-1mg
1 mg
$105.00
21 days
3269-5mg
5 mg
$295.00
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3269-50mg
50 mg
$2500.00
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BDP 558/568 DBCO is a bright and photostable dye with emission in the yellow spectrum range. It contains a cyclooctyne substituting group (i.e. DBCO), which can react with various azides in Click Chemistry reactions.
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Stable fluorophore that can be conjugated with trans-cyclooctene derivatives of biomolecules for their visualization.
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Tetrazine derivative of BDP 576/589 hydrophobic dye for conjugation with trans-cyclooctenes and cyclopropenes in the TCO ligation reaction.
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BDP 581/591 is a borondipyrromethene dye that can be used not only as a fluorescent dye, but also as a ROS probe. It can be conjugated with azides using copper-free click chemistry.
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BDP 581/591 is a borondipyrromethene dye with tetrazine group for the inverse electron demand Diels-Alder (IEDDA) ligation.
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164E0
1 mg
$110.00
in stock
264E0
5 mg
$290.00
in stock
464E0
25 mg
$690.00
in stock
564E0
50 mg
$1270.00
in stock
664E0
100 mg
$1990.00
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BDP 630/650 is a borondipyrromethene dye for Cyanine5 channel. This is a tetrazine derivative for the coupling with strained cycloalkenes.
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154E0
1 mg
$110.00
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254E0
5 mg
$290.00
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454E0
25 mg
$690.00
in stock
554E0
50 mg
$1270.00
in stock
654E0
100 mg
$1990.00
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This red-fluorescent dye contains a substituting group for copper-free click chemistry.
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BDP FL DBCO is a dye cyclooctyne for copper-free click chemistry reaction with azides. Absorption and emission wavelengths of BDP FL correspond to those of fluorescein (FAM), but BDP FL is significantly more photostable.
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BDP FL tetrazine (methyltetrazine) is a reactive dye for the inverse electron demand Diels-Alder (IEDDA) reaction with trans-cyclooctenes, cyclopropenes, and other strained cycloolefines. BDP FL is a bright and photostable dye for the 488 nm fluorescein (FAM) channel.
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114E0
1 mg
$110.00
in stock
214E0
5 mg
$290.00
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414E0
25 mg
$690.00
in stock
514E0
50 mg
$1270.00
in stock
614E0
100 mg
$1990.00
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A derivative of BDP R6G dye with azodibenzocyclooctyne (DBCO or ADIBO) for copper-free click chemistry conjugation with azides.
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BDP R6G tetrazine is a borondipyrromethene dye with absorption and emission wavelengths similar to rhodamine 6G. It contains tetrazine fragment for IEDDA ligation.
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144E0
1 mg
$110.00
in stock
244E0
5 mg
$290.00
in stock
444E0
25 mg
$690.00
in stock
544E0
50 mg
$1270.00
in stock
644E0
100 mg
$1990.00
in stock
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This yellow-orange-fluorescent boron dipyrromethene dye contains a DBCO substituting group for participation in click chemistry reactions.
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124F0
1 mg
$125.00
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224F0
5 mg
$260.00
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424F0
25 mg
$510.00
in stock
524F0
50 mg
$895.00
in stock
624F0
100 mg
$1490.00
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A fluorophore for the TAMRA channel with a functional group that can form conjugates in click chemistry reactions (IEDDA ligation) .
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124E0
1 mg
$125.00
in stock
224E0
5 mg
$260.00
in stock
424E0
25 mg
$510.00
in stock
524E0
50 mg
$895.00
in stock
624E0
100 mg
$1490.00
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A dye with a dibenzocyclooctyne substituting group for the ROX channel.
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1D4F0
1 mg
$125.00
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2D4F0
5 mg
$260.00
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4D4F0
25 mg
$510.00
in stock
5D4F0
50 mg
$895.00
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6D4F0
100 mg
$1490.00
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BDP TR is a borondipyrromethene fluorophore for ROX channel. The dye produces bright fluorescent emission. Tetrazines are reactive components for the conjugation with strained cycloalkenes.
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134E0
1 mg
$110.00
in stock
234E0
5 mg
$290.00
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434E0
25 mg
$690.00
in stock
534E0
50 mg
$1270.00
in stock
634E0
100 mg
$1990.00
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Biotin tetrazine is a reagent for copper-free click chemistry labeling with biotin, a well-known affinity probe to streptavidin.
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3797-25mg
25 mg
$210.00
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3797-250mg
250 mg
$1190.00
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Cyanine3 dibenzocyclooctyne (DBCO, or ADIBO) is a cycloalkyne dye derivative for copper-free click chemistry.
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Tetrazine derivative of the fluorophore Cyanine3. Tetrazines react extremely quickly with strained alkenes (such as trans-cyclooctenes, cyclopropenes), and some strained alkynes.
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110E0
1 mg
$125.00
in stock
210E0
5 mg
$260.00
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410E0
25 mg
$510.00
in stock
510E0
50 mg
$895.00
in stock
610E0
100 mg
$1490.00
in stock
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Cyclooctyne derivative of Cyanine3.5 dye, which easily reacts with various azides resulting in stable conjugates.
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Cyanine5 dye with dibenzocyclooctyne (DBCO) functional group for copper-free click chemistry.
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Cyanine5 is a universal red emitting dye with a very high extinction coefficient, and good quantum yield. This tetrazine derivative reacts very quickly with trans-cyclooctenes and cyclopropenes with the formation of stable conjugates.
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130E0
1 mg
$125.00
in stock
230E0
5 mg
$260.00
in stock
430E0
25 mg
$510.00
in stock
530E0
50 mg
$895.00
in stock
630E0
100 mg
$1490.00
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Cyanine5.5 is a far-red / NIR emitting dye that is useful for bioimaging studies. Cyanine5.5 DBCO is a derivative of the dye with a cycloalkyne moiety for copper-free click chemistry.
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A compound containing Cyanine5.5 fluorophore, a far red emitting dye, and tetrazine fragment for the conjugation with trans-cyclooctenes / cyclopropenes via TCO ligation.
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170E0
1 mg
$125.00
in stock
270E0
5 mg
$260.00
in stock
470E0
25 mg
$510.00
in stock
570E0
50 mg
$895.00
in stock
670E0
100 mg
$1490.00
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NIR fluorescent dye functionalized with cyclooctyne moiety for copper free click chemistry conjugation with azides.
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Near infrared Cyanine7 dye derivative containing tetrazine group for TCO ligation.
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150E0
1 mg
$125.00
in stock
250E0
5 mg
$325.00
in stock
450E0
25 mg
$510.00
in stock
550E0
50 mg
$895.00
in stock
650E0
100 mg
$1490.00
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Cyanine7.5 tetrazine is a tetrazine derivative for TCO ligation and Diels-Alder reaction containing a NIR fluorophore and suitable for in vivo infrared imaging.
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160E0
1 mg
$125.00
in stock
260E0
5 mg
$325.00
in stock
460E0
25 mg
$510.00
in stock
560E0
50 mg
$895.00
in stock
660E0
100 mg
$1490.00
in stock
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Bifunctional linker containing dibenzocyclooctyne (DBCO, ADIBO) for strain-promoted copper-free click reaction (SPAAC) and free amine for the coupling with various electrophilic compounds like activated esters and epoxides.
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1280-100mg
100 mg
$240.00
in stock
1280-500mg
500 mg
$600.00
in stock
1280-1g
1 g
$1200.00
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Azodibenzocyclooctyne (DBCO, ADIBO) reagent for strain-promoted copper-free click chemistry (SPAAC). The reagent contains an NHS ester function for the attachment of the cyclooctyne to various molecules.
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Activated ester for modification of proteins/antibodies/peptides followed by introduction into copper-free click chemistry reaction.
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A heterobifunctional linker with DBCO and NHS ester function groups flanking PEG6 (hexaethylene glycol).
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3664-50mg
50 mg
$247.00
in stock
3664-100mg
100 mg
$449.00
in stock
3664-1g
1 g
$3490.00
5 days
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BCN-containing phosphoramidite for the synthesis of oligonucleotides and their labeling using copper-free click reactions.
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3005-100mg
100 mg
$390.00
in stock
3005-250mg
250 mg
$800.00
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3005-1g
1 g
$2350.00
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Cyclooctyne derivative of fluorescein (FAM) for copper-free click chemistry reaction with azides. Pure 6-isomer of fluorescein.
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151F0
1 mg
$110.00
in stock
251F0
5 mg
$160.00
in stock
451F0
25 mg
$260.00
in stock
551F0
50 mg
$370.00
in stock
651F0
100 mg
$525.00
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FAM tetrazine (methyltetrazine) for the conjugation with trans-cyclooctenes and cyclopropenes. Pure 5-isomer of fluorescein.
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141E0
1 mg
$110.00
in stock
241E0
5 mg
$210.00
in stock
441E0
25 mg
$410.00
in stock
541E0
50 mg
$695.00
in stock
641E0
100 mg
$1190.00
in stock
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This molecule is a conjugate of bright fluorophore FAM (fluorescein) and tetrazine, a reactive group that reacts with trans-cycloalkenes in a reaction called IEDDA (inverse electron demand Diels-Alder reaction).
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151E0
1 mg
$110.00
in stock
251E0
5 mg
$210.00
in stock
451E0
25 mg
$410.00
in stock
551E0
50 mg
$695.00
in stock
651E0
100 mg
$1190.00
in stock
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PEG3 tetrazine is a mono-reactive triethyleneglycol with a tetrazine group for IEDDA conjugation with strained olefins.
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1978-250mg
250 mg
$390.00
in stock
1978-1g
1 g
$950.00
in stock
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ROX (rhodamine X) is a bright rhodamine dye. This is a DBCO (dibenzocyclooctyne) derivative that has high reactivity against azides.
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112F0
1 mg
$110.00
in stock
212F0
5 mg
$210.00
in stock
412F0
25 mg
$410.00
in stock
512F0
50 mg
$695.00
in stock
612F0
100 mg
$1190.00
in stock
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A tetrazine derivative of bright red-emitting dye ROX (Rhodamine X, Rhodamine 101) for copper-free bioconjugation. Pure 5-isomer.
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112E0
1 mg
$110.00
in stock
212E0
5 mg
$210.00
in stock
412E0
25 mg
$410.00
in stock
512E0
50 mg
$695.00
in stock
612E0
100 mg
$1190.00
in stock
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sulfo-Cyanine3 DBCO is a cycloalkyne derivative of water-soluble fluorophore sulfo-Cyanine3 for copper-free click chemistry.
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113F0
1 mg
$125.00
in stock
213F0
5 mg
$325.00
in stock
413F0
25 mg
$850.00
in stock
513F0
50 mg
$1490.00
in stock
613F0
100 mg
$1990.00
in stock
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Water soluble sulfo-Cyanine3 dye tetrazine derivative for TCO ligation labeling with trans-cyclooctenes.
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Water soluble cycloalkyne for copper-free click chemistry reaction with azides. A derivative of sulfo-Cyanine5.
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133F0
1 mg
$125.00
in stock
233F0
5 mg
$325.00
in stock
433F0
25 mg
$850.00
in stock
533F0
50 mg
$1490.00
in stock
633F0
100 mg
$1990.00
in stock
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Water soluble Cyanine dye tetrazine derivative for TCO ligation with trans-cyclooctenes and other strained olefines.
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Sulfo-Cyanine5.5 DBCO is a far-red, water-soluble reagent for copper-free click reaction with azides.
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173F0
1 mg
$125.00
in stock
273F0
5 mg
$325.00
in stock
473F0
25 mg
$850.00
in stock
573F0
50 mg
$1490.00
in stock
673F0
100 mg
$1990.00
in stock
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Tetrazine derivative of water-soluble far-red emitting fluorescent dye sulfo-Cyanine5.5 for TCO ligation with trans-cyclooctenes and other strained olefins.
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173E0
1 mg
$125.00
in stock
273E0
5 mg
$325.00
in stock
473E0
25 mg
$850.00
in stock
573E0
50 mg
$1490.00
in stock
673E0
100 mg
$1990.00
in stock
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Dibenzocyclooctyne (DBCO, or ADIBO) conjugated with sulfo-Cyanine7, a water-soluble near-IR dye for labeling azide-containing biomolecules inside living cells, whole organisms, and inanimate samples.
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153F0
1 mg
$125.00
in stock
253F0
5 mg
$325.00
in stock
453F0
25 mg
$850.00
in stock
553F0
50 mg
$1490.00
in stock
653F0
100 mg
$1990.00
in stock
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United States , ZIP
Items in stock will be shipped on
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Near infrared, water soluble dye methyltetrazine for the conjugation with strained cycloalkenes in TCO ligation reaction.
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153E0
1 mg
$125.00
in stock
253E0
5 mg
$325.00
in stock
453E0
25 mg
$850.00
in stock
553E0
50 mg
$1490.00
in stock
653E0
100 mg
$1990.00
in stock
Delivery options for stock items to
United States , ZIP
Items in stock will be shipped on
03/19/2024
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Cyclooctyne derivative of tetramethylrhodamine (TMR, TAMRA) for copper-free click chemistry conjugation with azides. Pure 5-isomer.
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171F0
1 mg
$125.00
5 days
271F0
5 mg
$395.00
in stock
471F0
25 mg
$890.00
in stock
571F0
50 mg
$1490.00
in stock
671F0
100 mg
$1990.00
in stock
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United States , ZIP
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Trans-cyclooctene (TCO) amidite is used to introduce a TCO moiety into substrates containing primary or secondary hydroxyl group
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4017-250mg
250 mg
$920.00
in stock
4017-1g
1 g
please inquire
12 days
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A heterobifunctional linker with trans-cyclooctene and NHS ester groups flanking PEG4 (tetraethylene glycol) for labeling of antibodies, proteins, and other primary amine-containing biomolecules.
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3056-10mg
10 mg
$145.00
in stock
3056-25mg
25 mg
$225.00
in stock
3056-100mg
100 mg
$550.00
in stock
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VdU is a synthetic analog of thymidine for studying de novo DNA synthesis and cell proliferation.
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D2540
25 mg
$225.00
in stock
F2540
100 mg
$785.00
in stock
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Uridine derivative with a terminal alkene group for monitoring and investigating cell RNA transcription.
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3517-10mg
10 mg
$125.00
in stock
3517-50mg
50 mg
$449.00
in stock
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United States , ZIP
Items in stock will be shipped on
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