Copper-free click chemistry in Applications
Copper-free click chemistry reactions include cycloadditions for bioconjugation that do not rely on the use of transition metal catalyst, as opposed to traditional copper catalyzed сlick chemistry.
These reactions include the strain promoted alkyne-azide cycloaddition (SPAAC) that involves strained cycloalkyne, and azide. Relief of the sterical strain provides a driving force for this reaction.
DBCO (ADIBO) cycloalkynes are among the most reactive yet stable. The reaction yields two regioisomers that usually cannot be separated, therefore this does not lead to peak doubling in HPLC.
Another process is TCO ligation (tetrazine - trans-cyclooctene ligation) which is also powered by sterical strain. Unlike SPAAC, this is [4+2]-cycloaddition yielding 6-membered ring. TCO ligation is among the fastest reactions used for the bioconjugation.
Both these reactions are very biocompatible as they do not require to use a metal catalyst, they are fast, and orthogonal in case of DBCO cycloalkynes (i.e., it is possible to run two reaction in one mixture without interference).
Lumiprobe manufactures a number of reagents for copper-free click-chemistry, for both SPAAC, and TCO ligation.
4AZP (4-Azido-L-phenylalanine)
Unnatural L-phenylalanine derivative with an azide moiety for nascent protein synthesis detection via copper-catalyzed and copper-free click chemistry.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3415-100mg | 100 mg | $125.00 | in stock | |
| 3415-500mg | 500 mg |
$625.00
|
in stock |
Ac4GalNAz (N-Azidoacetylgalactosamine-tetraacylated)
An azide-functionalized monosaccharide for metabolic labeling and studying the cellular trafficking of labeled proteins and proteomic analysis.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3706-5mg | 5 mg | $129.00 | in stock | |
| 3706-25mg | 25 mg | $240.00 | in stock | |
| 3706-100mg | 100 mg |
$630.00
|
in stock |
Ac4GlcNAz (N-Azidoacetylglucosamine-tetraacylated)
An azide-functionalized monosaccharide for metabolic labeling and studying the cellular trafficking of labeled proteins and proteomic analysis.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3662-5mg | 5 mg | $129.00 | in stock | |
| 3662-25mg | 25 mg | $240.00 | in stock | |
| 3662-100mg | 100 mg |
$630.00
|
in stock |
Ac4ManNAz (N-Azidoacetylmannosamine-tetraacylated)
An azide-functionalized monosaccharide for metabolic labeling and studying the cellular trafficking of labeled proteins and proteomic analysis.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3159-5mg | 5 mg | $129.00 | in stock | |
| 3159-25mg | 25 mg | $240.00 | in stock | |
| 3159-100mg | 100 mg |
$630.00
|
in stock |
AF 430 tetrazine
Tetrazine derivative of AF 430 fluorescent dye suitable for in vitro cell labeling.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 108E0 | 1 mg | $125.00 | in stock | |
| 208E0 | 5 mg | $325.00 | in stock | |
| 308E0 | 10 mg |
$480.00
|
in stock | |
| 408E0 | 25 mg |
$950.00
|
in stock | |
| 508E0 | 50 mg |
$1625.00
|
in stock | |
| 608E0 | 100 mg |
$2090.00
|
in stock |
AF 488 DBCO
Dibenzocyclooctyne (DBCO, ADIBO) containing the green fluorophore AF 488 for labeling azide-tagged biomolecules inside live cells, whole organisms, and inanimate samples. Pure 5-isomer.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 118F0 | 1 mg | $140.00 | in stock | |
| 218F0 | 5 mg |
$450.00
|
in stock | |
| 418F0 | 25 mg |
$1460.00
|
in stock | |
| 518F0 | 50 mg |
$1890.00
|
in stock | |
| 618F0 | 100 mg |
$2640.00
|
in stock |
AF 568 DBCO
Dibenzocyclooctyne (DBCO, ADIBO) containing the orange fluorophore AF 568 for cooper-free labeling azide-tagged biomolecules inside live cells, whole organisms, and inanimate samples.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1158F0 | 1 mg | $140.00 | in stock | |
| 2158F0 | 5 mg |
$450.00
|
in stock | |
| 4158F0 | 25 mg |
$1460.00
|
in stock | |
| 5158F0 | 50 mg |
$1890.00
|
in stock | |
| 6158F0 | 100 mg |
$2990.00
|
in stock |
AHA (L-Azidohomoalanine)
Noncanonical amino acid analog of methionine with an azide moiety for nascent protein synthesis detection via copper-catalyzed and copper-free click chemistry.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3799-25mg | 25 mg | $125.00 | 21 days | |
| 3799-100mg | 100 mg | $235.00 | in stock |
AmdU (5-Azidomethyl-2'-deoxyuridine)
Azidomethyldeoxyuridine (AmdU) is an azide-contained nucleoside that is incorporated into nascent DNA by cellular polymerases. The azide groups attached to DNA this way can be subsequently modified with alkynes or cycloalkynes via copper-catalyzed or strain-promoted click reaction.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| B1540 | 5 mg | $120.00 | in stock | |
| D1540 | 25 mg | $255.00 | in stock | |
| F1540 | 100 mg |
$890.00
|
in stock |
Azide-PEG3-tetrazine
A hydrophilic bifunctional linker with azide and tetrazine function groups flanking PEG3 (triethylene glycol) spacer.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2798-250mg | 250 mg |
$549.00
|
in stock |
BCN-PEG4-Cyanine3 (exo)
BCN-PEG4-Cyanine3 is an ethylene glycol separated linker for the azido-modified molecules for copper-free click conjugation.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3269-1mg | 1 mg | $105.00 | 21 days | |
| 3269-5mg | 5 mg | $295.00 | in stock | |
| 3269-50mg | 50 mg |
$2500.00
|
in stock |
BDP 558/568 DBCO
BDP 558/568 DBCO is a bright and photostable dye with emission in the yellow spectrum range. It contains a cyclooctyne substituting group (i.e. DBCO), which can react with various azides in Click Chemistry reactions.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 174F0 | 1 mg | $125.00 | in stock | |
| 274F0 | 5 mg | $260.00 | in stock | |
| 374F0 | 10 mg | $325.00 | in stock | |
| 474F0 | 25 mg |
$510.00
|
in stock | |
| 574F0 | 50 mg |
$895.00
|
in stock | |
| 674F0 | 100 mg |
$1490.00
|
in stock |
BDP 558/568 tetrazine
Stable fluorophore that can be conjugated with trans-cyclooctene derivatives of biomolecules for their visualization.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A74E0 | 1 mg | $125.00 | in stock | |
| B74E0 | 5 mg | $260.00 | in stock | |
| C74E0 | 10 mg | $325.00 | in stock | |
| D74E0 | 25 mg |
$510.00
|
in stock | |
| E74E0 | 50 mg |
$895.00
|
in stock | |
| F74E0 | 100 mg |
$1490.00
|
in stock |
BDP 576/589 tetrazine
Tetrazine derivative of BDP 576/589 hydrophobic dye for conjugation with trans-cyclooctenes and cyclopropenes in the TCO ligation reaction.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1G4E0 | 1 mg | $110.00 | in stock | |
| 2G4E0 | 5 mg | $290.00 | in stock | |
| 3G4E0 | 10 mg |
$450.00
|
in stock | |
| 4G4E0 | 25 mg |
$690.00
|
in stock | |
| 5G4E0 | 50 mg |
$1270.00
|
in stock | |
| 6G4E0 | 100 mg |
$1990.00
|
in stock |
BDP 581/591 DBCO
BDP 581/591 is a borondipyrromethene dye that can be used not only as a fluorescent dye, but also as a ROS probe. It can be conjugated with azides using copper-free click chemistry.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A64F0 | 1 mg | $125.00 | in stock | |
| B64F0 | 5 mg | $260.00 | in stock | |
| C64F0 | 10 mg | $325.00 | in stock | |
| D64F0 | 25 mg |
$510.00
|
in stock | |
| E64F0 | 50 mg |
$895.00
|
in stock | |
| F64F0 | 100 mg |
$1490.00
|
in stock |
BDP 581/591 tetrazine
BDP 581/591 is a borondipyrromethene dye with tetrazine group for the inverse electron demand Diels-Alder (IEDDA) ligation.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 164E0 | 1 mg | $110.00 | in stock | |
| 264E0 | 5 mg | $290.00 | in stock | |
| 464E0 | 25 mg |
$690.00
|
in stock | |
| 564E0 | 50 mg |
$1270.00
|
in stock | |
| 664E0 | 100 mg |
$1990.00
|
in stock |
BDP 630/650 tetrazine
BDP 630/650 is a borondipyrromethene dye for Cyanine5 channel. This is a tetrazine derivative for the coupling with strained cycloalkenes.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 154E0 | 1 mg | $110.00 | in stock | |
| 254E0 | 5 mg | $290.00 | in stock | |
| 454E0 | 25 mg |
$690.00
|
in stock | |
| 554E0 | 50 mg |
$1270.00
|
in stock | |
| 654E0 | 100 mg |
$1990.00
|
in stock |
BDP 650/665 DBCO
This red-fluorescent dye contains a substituting group for copper-free click chemistry.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| AE4F0 | 1 mg | $125.00 | in stock | |
| BE4F0 | 5 mg | $260.00 | in stock | |
| CE4F0 | 10 mg | $325.00 | in stock | |
| DE4F0 | 25 mg |
$510.00
|
in stock | |
| EE4F0 | 50 mg |
$895.00
|
in stock | |
| FE4F0 | 100 mg |
$1490.00
|
in stock |
BDP FL DBCO
BDP FL DBCO is a dye cyclooctyne for copper-free click chemistry reaction with azides. Absorption and emission wavelengths of BDP FL correspond to those of fluorescein (FAM), but BDP FL is significantly more photostable.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A14F0 | 1 mg | $125.00 | in stock | |
| B14F0 | 5 mg | $260.00 | in stock | |
| C14F0 | 10 mg | $325.00 | in stock | |
| D14F0 | 25 mg |
$510.00
|
in stock | |
| E14F0 | 50 mg |
$895.00
|
in stock | |
| F14F0 | 100 mg |
$1490.00
|
in stock |
BDP FL tetrazine
BDP FL tetrazine (methyltetrazine) is a reactive dye for the inverse electron demand Diels-Alder (IEDDA) reaction with trans-cyclooctenes, cyclopropenes, and other strained cycloolefines. BDP FL is a bright and photostable dye for the 488 nm fluorescein (FAM) channel.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 114E0 | 1 mg | $110.00 | in stock | |
| 214E0 | 5 mg | $290.00 | in stock | |
| 414E0 | 25 mg |
$690.00
|
in stock | |
| 514E0 | 50 mg |
$1270.00
|
in stock | |
| 614E0 | 100 mg |
$1990.00
|
in stock |
BDP R6G DBCO
A derivative of BDP R6G dye with azodibenzocyclooctyne (DBCO or ADIBO) for copper-free click chemistry conjugation with azides.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A44F0 | 1 mg | $125.00 | in stock | |
| B44F0 | 5 mg | $260.00 | in stock | |
| C44F0 | 10 mg | $325.00 | in stock | |
| D44F0 | 25 mg |
$510.00
|
in stock | |
| E44F0 | 50 mg |
$895.00
|
in stock | |
| F44F0 | 100 mg |
$1490.00
|
in stock |
BDP R6G tetrazine
BDP R6G tetrazine is a borondipyrromethene dye with absorption and emission wavelengths similar to rhodamine 6G. It contains tetrazine fragment for IEDDA ligation.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 144E0 | 1 mg | $110.00 | in stock | |
| 244E0 | 5 mg | $290.00 | in stock | |
| 444E0 | 25 mg |
$690.00
|
in stock | |
| 544E0 | 50 mg |
$1270.00
|
in stock | |
| 644E0 | 100 mg |
$1990.00
|
in stock |
BDP TMR DBCO
This yellow-orange-fluorescent boron dipyrromethene dye contains a DBCO substituting group for participation in click chemistry reactions.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 124F0 | 1 mg | $125.00 | in stock | |
| 224F0 | 5 mg | $260.00 | in stock | |
| 424F0 | 25 mg |
$510.00
|
in stock | |
| 524F0 | 50 mg |
$895.00
|
in stock | |
| 624F0 | 100 mg |
$1490.00
|
in stock |
BDP TMR tetrazine
A fluorophore for the TAMRA channel with a functional group that can form conjugates in click chemistry reactions (IEDDA ligation) .
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 124E0 | 1 mg | $125.00 | in stock | |
| 224E0 | 5 mg | $260.00 | in stock | |
| 424E0 | 25 mg |
$510.00
|
in stock | |
| 524E0 | 50 mg |
$895.00
|
in stock | |
| 624E0 | 100 mg |
$1490.00
|
in stock |
BDP TR DBCO
A dye with a dibenzocyclooctyne substituting group for the ROX channel.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1D4F0 | 1 mg | $125.00 | in stock | |
| 2D4F0 | 5 mg | $260.00 | in stock | |
| 4D4F0 | 25 mg |
$510.00
|
in stock | |
| 5D4F0 | 50 mg |
$895.00
|
in stock | |
| 6D4F0 | 100 mg |
$1490.00
|
in stock |
BDP TR tetrazine
BDP TR is a borondipyrromethene fluorophore for ROX channel. The dye produces bright fluorescent emission. Tetrazines are reactive components for the conjugation with strained cycloalkenes.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 134E0 | 1 mg | $110.00 | in stock | |
| 234E0 | 5 mg | $290.00 | in stock | |
| 434E0 | 25 mg |
$690.00
|
in stock | |
| 534E0 | 50 mg |
$1270.00
|
in stock | |
| 634E0 | 100 mg |
$1990.00
|
in stock |
Biotin-PEG4-tetrazine
Biotin tetrazine is a reagent for copper-free click chemistry labeling with biotin, a well-known affinity probe to streptavidin.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3797-25mg | 25 mg | $210.00 | in stock | |
| 3797-250mg | 250 mg |
$1190.00
|
in stock |
Cyanine3 DBCO
Cyanine3 dibenzocyclooctyne (DBCO, or ADIBO) is a cycloalkyne dye derivative for copper-free click chemistry.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A10F0 | 1 mg | $125.00 | in stock | |
| B10F0 | 5 mg | $260.00 | in stock | |
| C10F0 | 10 mg | $325.00 | in stock | |
| D10F0 | 25 mg |
$510.00
|
in stock | |
| E10F0 | 50 mg |
$895.00
|
in stock | |
| F10F0 | 100 mg |
$1490.00
|
in stock |
Cyanine3 tetrazine
Tetrazine derivative of the fluorophore Cyanine3. Tetrazines react extremely quickly with strained alkenes (such as trans-cyclooctenes, cyclopropenes), and some strained alkynes.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 110E0 | 1 mg | $125.00 | in stock | |
| 210E0 | 5 mg | $260.00 | in stock | |
| 410E0 | 25 mg |
$510.00
|
in stock | |
| 510E0 | 50 mg |
$895.00
|
in stock | |
| 610E0 | 100 mg |
$1490.00
|
in stock |
Cyanine3.5 DBCO
Cyclooctyne derivative of Cyanine3.5 dye, which easily reacts with various azides resulting in stable conjugates.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A20F0 | 1 mg | $125.00 | in stock | |
| B20F0 | 5 mg | $260.00 | in stock | |
| C20F0 | 10 mg | $325.00 | in stock | |
| D20F0 | 25 mg |
$510.00
|
in stock | |
| E20F0 | 50 mg |
$895.00
|
in stock | |
| F20F0 | 100 mg |
$1490.00
|
in stock |
Cyanine5 DBCO
Cyanine5 dye with dibenzocyclooctyne (DBCO) functional group for copper-free click chemistry.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A30F0 | 1 mg | $125.00 | 5 days | |
| B30F0 | 5 mg | $260.00 | 5 days | |
| C30F0 | 10 mg | $325.00 | in stock | |
| D30F0 | 25 mg |
$510.00
|
in stock | |
| E30F0 | 50 mg |
$895.00
|
in stock | |
| F30F0 | 100 mg |
$1490.00
|
in stock |
Cyanine5 tetrazine
Cyanine5 is a universal red emitting dye with a very high extinction coefficient, and good quantum yield. This tetrazine derivative reacts very quickly with trans-cyclooctenes and cyclopropenes with the formation of stable conjugates.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 130E0 | 1 mg | $125.00 | in stock | |
| 230E0 | 5 mg | $260.00 | in stock | |
| 430E0 | 25 mg |
$510.00
|
in stock | |
| 530E0 | 50 mg |
$895.00
|
in stock | |
| 630E0 | 100 mg |
$1490.00
|
in stock |
Cyanine5.5 DBCO
Cyanine5.5 is a far-red / NIR emitting dye that is useful for bioimaging studies. Cyanine5.5 DBCO is a derivative of the dye with a cycloalkyne moiety for copper-free click chemistry.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A70F0 | 1 mg | $125.00 | in stock | |
| B70F0 | 5 mg | $260.00 | in stock | |
| C70F0 | 10 mg | $325.00 | in stock | |
| D70F0 | 25 mg |
$510.00
|
in stock | |
| E70F0 | 50 mg |
$895.00
|
in stock | |
| F70F0 | 100 mg |
$1490.00
|
in stock |
Cyanine5.5 tetrazine
A compound containing Cyanine5.5 fluorophore, a far red emitting dye, and tetrazine fragment for the conjugation with trans-cyclooctenes / cyclopropenes via TCO ligation.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 170E0 | 1 mg | $125.00 | in stock | |
| 270E0 | 5 mg | $260.00 | in stock | |
| 470E0 | 25 mg |
$510.00
|
in stock | |
| 570E0 | 50 mg |
$895.00
|
in stock | |
| 670E0 | 100 mg |
$1490.00
|
in stock |
Cyanine7 DBCO
NIR fluorescent dye functionalized with cyclooctyne moiety for copper free click chemistry conjugation with azides.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A50F0 | 1 mg | $125.00 | in stock | |
| B50F0 | 5 mg | $260.00 | in stock | |
| C50F0 | 10 mg | $325.00 | in stock | |
| D50F0 | 25 mg |
$510.00
|
in stock | |
| E50F0 | 50 mg |
$895.00
|
in stock | |
| F50F0 | 100 mg |
$1490.00
|
in stock |
Cyanine7 tetrazine
Near infrared Cyanine7 dye derivative containing tetrazine group for TCO ligation.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 150E0 | 1 mg | $125.00 | in stock | |
| 250E0 | 5 mg | $325.00 | in stock | |
| 450E0 | 25 mg |
$510.00
|
in stock | |
| 550E0 | 50 mg |
$895.00
|
in stock | |
| 650E0 | 100 mg |
$1490.00
|
in stock |