Copper free Click chemistry in Applications
Copper free Click chemistry reactions include cycloadditions for bioconjugation that do not rely on the use of transition metal catalyst, as opposed to traditional copper catalyzed Click chemistry.
These reactions include the strain promoted alkyne-azide cycloaddition (spAAc) that involves strained cycloalkyne, and azide. Relief of the sterical strain provides a driving force for this reaction.
DBCO (ADIBO) cycloalkynes are among the most reactive yet stable. The reaction yields two regioisomers that usually cannot be separated, therefore this does not lead to peak doubling in HPLC.
Another process is TCO ligation (tetrazine - trans-cyclooctene ligation) which is also powered by sterical strain. Unlike spAAc, this is [4+2]-cycloaddition yielding 6-membered ring. TCO ligation is among the fastest reactions used for the bioconjugation.
Both these reactions are very biocompatible as they do not require to use a metal catalyst, they are fast, and orthogonal in case of DBCO cycloalkynes (i.e., it is possible to run two reaction in one mixture without interference).
Lumiprobe manufactures a number of reagents for copper free Click chemistry, for both spAAc, and TCO ligation.
AmdU (5-azidomethyl-2'-deoxyuridine)
Azidomethyldeoxyuridine (AmdU) is an azide containing nucleoside that is incorporated into nascent DNA by cellular polymerases. The azide groups attached to DNA this way can be subsequently modified with alkynes or cycloalkynes via copper-catalyzed or strain promoted Click chemistry.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| B1540 | 5 mg | $120.00 | in stock | |
| D1540 | 25 mg | $255.00 | in stock | |
| F1540 | 100 mg |
$890.00
|
in stock |
BDP 630/650 tetrazine
BDP 630/650 is a borondipyrromethene dye for Cyanine5 channel. This is a tetrazine derivative for the coupling with strained cycloalkenes.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 154E0 | 1 mg | $110.00 | in stock | |
| 254E0 | 5 mg | $290.00 | in stock | |
| 454E0 | 25 mg |
$690.00
|
in stock | |
| 554E0 | 50 mg |
$1270.00
|
in stock | |
| 654E0 | 100 mg |
$1990.00
|
in stock |
BDP FL DBCO
BDP FL DBCO is a dye cyclooctyne for copper free Click chemistry reaction with azides. Absorption and emission wavelengths of BDP FL correspond to those of fluorescein (FAM), but BDP FL is significantly more photostable.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A14F0 | 1 mg | $110.00 | in stock | |
| B14F0 | 5 mg | $210.00 | in stock | |
| C14F0 | 10 mg | $310.00 | in stock | |
| D14F0 | 25 mg |
$410.00
|
in stock | |
| E14F0 | 50 mg |
$695.00
|
in stock | |
| F14F0 | 100 mg |
$1190.00
|
in stock |
BDP FL tetrazine
BDP FL tetrazine (methyltetrazine) is a reactive dye for the inverse electron demand Diels-Alder (ied-DA) reaction with trans-cyclooctenes, cyclopropenes, and other strained cycloolefines. BDP FL is a bright and photostable dye for the 488 nm fluorescein (FAM) channel.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 114E0 | 1 mg | $110.00 | in stock | |
| 214E0 | 5 mg | $290.00 | in stock | |
| 414E0 | 25 mg |
$690.00
|
in stock | |
| 514E0 | 50 mg |
$1270.00
|
in stock | |
| 614E0 | 100 mg |
$1990.00
|
in stock |
BDP R6G tetrazine
BDP R6G tetrazine is a borondipyrromethene dye with absorption and emission wavelengths similar to rhodamine 6G. It contains tetrazine fragment for iedDA ligation.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 144E0 | 1 mg | $110.00 | in stock | |
| 244E0 | 5 mg | $290.00 | in stock | |
| 444E0 | 25 mg |
$690.00
|
in stock | |
| 544E0 | 50 mg |
$1270.00
|
in stock | |
| 644E0 | 100 mg |
$1990.00
|
in stock |
Cyanine3 DBCO
Cyanine3 dibenzocyclooctyne (DBCO, or ADIBO) is a cycloalkyne dye derivative for copper free Click chemistry.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A10F0 | 1 mg | $110.00 | in stock | |
| B10F0 | 5 mg | $210.00 | in stock | |
| C10F0 | 10 mg | $310.00 | in stock | |
| D10F0 | 25 mg |
$410.00
|
in stock | |
| E10F0 | 50 mg |
$695.00
|
in stock | |
| F10F0 | 100 mg |
$1190.00
|
in stock |
Cyanine3 tetrazine
Tetrazine derivative of the fluorophore Cyanine3. Tetrazines react extremely quickly with strained alkenes (such as trans-cyclooctenes, cyclopropenes), and some strained alkynes.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 110E0 | 1 mg | $110.00 | in stock | |
| 210E0 | 5 mg | $290.00 | in stock | |
| 410E0 | 25 mg |
$690.00
|
in stock | |
| 510E0 | 50 mg |
$1270.00
|
in stock | |
| 610E0 | 100 mg |
$1990.00
|
in stock |
Cyanine5 DBCO
Cyanine5 dye with dibenzocyclooctyne (DBCO) functional group for copper free Click chemistry.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A30F0 | 1 mg | $110.00 | in stock | |
| B30F0 | 5 mg | $210.00 | in stock | |
| C30F0 | 10 mg | $310.00 | in stock | |
| D30F0 | 25 mg |
$410.00
|
in stock | |
| E30F0 | 50 mg |
$695.00
|
in stock | |
| F30F0 | 100 mg |
$1190.00
|
in stock |
Cyanine5 tetrazine
Cyanine5 is a universal red emitting dye with a very high extinction coefficient, and good quantum yield. This tetrazine derivative reacts very quickly with trans-cyclooctenes and cyclopropenes with the formation of stable conjugates.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 130E0 | 1 mg | $110.00 | in stock | |
| 230E0 | 5 mg | $290.00 | in stock | |
| 430E0 | 25 mg |
$690.00
|
in stock | |
| 530E0 | 50 mg |
$1270.00
|
in stock | |
| 630E0 | 100 mg |
$1990.00
|
in stock |
Cyanine5.5 tetrazine
A compound containing Cyanine5.5 fluorophore, a far red emitting dye, and tetrazine fragment for the conjugation with trans-cyclooctenes / cyclopropenes via TCO ligation.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 170E0 | 1 mg | $110.00 | in stock | |
| 270E0 | 5 mg | $290.00 | in stock | |
| 470E0 | 25 mg |
$690.00
|
in stock | |
| 570E0 | 50 mg |
$1270.00
|
in stock | |
| 670E0 | 100 mg |
$1990.00
|
in stock |
Cyanine7 DBCO
NIR fluorescent dye functionalized with cyclooctyne moiety for copper free Click chemistry conjugation with azides.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A50F0 | 1 mg | $110.00 | in stock | |
| B50F0 | 5 mg | $210.00 | in stock | |
| C50F0 | 10 mg | $310.00 | in stock | |
| D50F0 | 25 mg |
$410.00
|
in stock | |
| E50F0 | 50 mg |
$695.00
|
in stock | |
| F50F0 | 100 mg |
$1190.00
|
in stock |
Cyanine7 tetrazine
Near infrared Cyanine7 dye derivative containing tetrazine group for TCO ligation.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 150E0 | 1 mg | $110.00 | in stock | |
| 250E0 | 5 mg | $290.00 | in stock | |
| 450E0 | 25 mg |
$690.00
|
in stock | |
| 550E0 | 50 mg |
$1270.00
|
in stock | |
| 650E0 | 100 mg |
$1990.00
|
in stock |
Cyanine7.5 tetrazine
Cyanine7.5 is a NIR fluorescent dye with absorption and emission spectra similar to ICG (indocyanine green), but possessing a higher fluorescence quantum yield. This derivative is a tetrazine derivative for the TCO ligation reaction.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 160E0 | 1 mg | $110.00 | in stock | |
| 260E0 | 5 mg | $290.00 | in stock | |
| 460E0 | 25 mg |
$690.00
|
in stock | |
| 560E0 | 50 mg |
$1270.00
|
in stock | |
| 660E0 | 100 mg |
$1990.00
|
in stock |
DBCO NHS ester
Azodibenzocyclooctyne (DBCO, ADIBO) reagent for strain promoted copper free Click chemistry (spAAC). The reagent contains an NHS ester function for the attachment of the cyclooctyne to various molecules.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 34720 | 10 mg | $60.00 | in stock | |
| 44720 | 25 mg | $95.00 | in stock | |
| 54720 | 50 mg | $145.00 | in stock | |
| 64720 | 100 mg | $210.00 | in stock | |
| 94720 | 1 g |
$1190.00
|
in stock |
FAM DBCO, 6-isomer
Cyclooctyne derivative of fluorescein (FAM) for copper free Click chemistry reaction with azides. Contains pure 6-isomer of fluorescein.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 151F0 | 1 mg | $110.00 | in stock | |
| 251F0 | 5 mg | $160.00 | in stock | |
| 451F0 | 25 mg | $210.00 | in stock | |
| 551F0 | 50 mg | $270.00 | in stock | |
| 651F0 | 100 mg |
$420.00
|
in stock |
FAM tetrazine, 5-isomer
FAM tetrazine (methyltetrazine) for the conjugation with trans-cyclooctenes and cyclopropenes. Pure 5-isomer of fluorescein.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 141E0 | 1 mg | $110.00 | in stock | |
| 241E0 | 5 mg | $210.00 | in stock | |
| 441E0 | 25 mg |
$410.00
|
in stock | |
| 541E0 | 50 mg |
$695.00
|
in stock | |
| 641E0 | 100 mg |
$1190.00
|
in stock |
FAM tetrazine, 6-isomer
This molecule is a conjugate of FAM (fluorescein) - a bright fluorophore, and tetrazine - a reactive group that reacts with trans-cycloalkenes in a reaction called ied-DA (inverse electron demand Diels Alder reaction).
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 151E0 | 1 mg | $110.00 | in stock | |
| 251E0 | 5 mg | $210.00 | in stock | |
| 451E0 | 25 mg |
$410.00
|
in stock | |
| 551E0 | 50 mg |
$695.00
|
in stock | |
| 651E0 | 100 mg |
$1190.00
|
in stock |
sulfo-Cyanine3 DBCO
Sulfo-Cyanine3 DBCO is a cycloalkyne derivative of water soluble, general purpose fluorophore sulfo-Cyanine3 for Click Chemistry.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 113F0 | 1 mg | $110.00 | in stock | |
| 213F0 | 5 mg | $290.00 | in stock | |
| 413F0 | 25 mg |
$690.00
|
in stock | |
| 513F0 | 50 mg |
$1270.00
|
in stock | |
| 613F0 | 100 mg |
$1990.00
|
in stock |
Sulfo-Cyanine3 tetrazine
Water soluble sulfo-Cyanine3 dye tetrazine derivative for TCO ligation labeling with trans-cyclooctenes.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 113E0 | 1 mg | $110.00 | in stock | |
| 213E0 | 5 mg | $290.00 | in stock | |
| 313E0 | 10 mg |
$460.00
|
in stock | |
| 413E0 | 25 mg |
$690.00
|
in stock | |
| 513E0 | 50 mg |
$1270.00
|
in stock | |
| 613E0 | 100 mg |
$1990.00
|
in stock |
sulfo-Cyanine5 DBCO
Water soluble cycloalkyne for copper free Click chemistry reaction with azides. A derivative of sulfo-Cyanine5 aka Cy5.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 133F0 | 1 mg | $110.00 | in stock | |
| 233F0 | 5 mg | $290.00 | in stock | |
| 433F0 | 25 mg |
$690.00
|
in stock | |
| 533F0 | 50 mg |
$1270.00
|
in stock | |
| 633F0 | 100 mg |
$1990.00
|
in stock |
Sulfo-Cyanine5 tetrazine
Water soluble Cyanine dye tetrazine derivative for TCO ligation with trans-cyclooctenes and other strained olefines.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 133E0 | 1 mg | $110.00 | in stock | |
| 233E0 | 5 mg | $290.00 | in stock | |
| 333E0 | 10 mg |
$460.00
|
in stock | |
| 433E0 | 25 mg |
$690.00
|
in stock | |
| 533E0 | 50 mg |
$1270.00
|
in stock | |
| 633E0 | 100 mg |
$1990.00
|
in stock |
Sulfo-Cyanine7 tetrazine
Near infrared, water soluble dye methyltetrazine for the conjugation with strained cycloalkenes in TCO ligation reaction.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 153E0 | 1 mg | $110.00 | in stock | |
| 253E0 | 5 mg | $290.00 | in stock | |
| 453E0 | 25 mg |
$690.00
|
in stock | |
| 553E0 | 50 mg |
$1270.00
|
in stock | |
| 653E0 | 100 mg |
$1990.00
|
in stock |
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