Copper-free click chemistry in Applications

Copper-free click chemistry reactions include cycloadditions for bioconjugation that do not rely on the use of transition metal catalyst, as opposed to traditional copper catalyzed сlick chemistry.

These reactions include the strain promoted alkyne-azide cycloaddition (SPAAC) that involves strained cycloalkyne, and azide. Relief of the sterical strain provides a driving force for this reaction.

Scheme of SPAAC copper-free click chemistry reaction

DBCO (ADIBO) cycloalkynes are among the most reactive yet stable. The reaction yields two regioisomers that usually cannot be separated, therefore this does not lead to peak doubling in HPLC.

Another process is TCO ligation (tetrazine - trans-cyclooctene ligation) which is also powered by sterical strain. Unlike SPAAC, this is [4+2]-cycloaddition yielding 6-membered ring. TCO ligation is among the fastest reactions used for the bioconjugation.

Scheme of TCO (trans-cyclooctene tetrazine) ligation reaction

Both these reactions are very biocompatible as they do not require to use a metal catalyst, they are fast, and orthogonal in case of DBCO cycloalkynes (i.e., it is possible to run two reaction in one mixture without interference).

Lumiprobe manufactures a number of reagents for copper-free click-chemistry, for both SPAAC, and TCO ligation.

Ac4GlcNAz (N-Azidoacetylglucosamine-tetraacylated)

An azide-functionalized monosaccharide for metabolic labeling and studying the cellular trafficking of labeled proteins and proteomic analysis.
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Cat. # Quantity Price Lead time
3662-5mg 5 mg $90.00 12 days
3662-25mg 25 mg $190.00 12 days
3662-100mg 100 mg $580.00 12 days

Ac4GalNAz (N-Azidoacetylgalactosamine-tetraacylated)

An azide-functionalized monosaccharide for metabolic labeling and studying the cellular trafficking of labeled proteins and proteomic analysis.
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Cat. # Quantity Price Lead time
3706-5mg 5 mg $90.00 in stock
3706-25mg 25 mg $190.00 in stock
3706-100mg 100 mg $580.00 in stock

Ac4ManNAz (N-Azidoacetylmannosamine-tetraacylated)

An azide-functionalized monosaccharide for metabolic labeling and studying the cellular trafficking of labeled proteins and proteomic analysis.
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Cat. # Quantity Price Lead time
3159-5mg 5 mg $90.00 in stock
3159-25mg 25 mg $190.00 in stock
3159-100mg 100 mg $580.00 in stock

AF 430 tetrazine

Tetrazine derivative of AF 430 fluorescent dye suitable for in vitro cell labeling.
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Cat. # Quantity Price Lead time
108E0 1 mg $110.00 in stock
208E0 5 mg $290.00 in stock
308E0 10 mg $460.00 in stock
408E0 25 mg $690.00 in stock
508E0 50 mg $1270.00 in stock
608E0 100 mg $1990.00 in stock

AF 488 DBCO, 5-isomer

Dibenzocyclooctyne (DBCO, ADIBO) containing the green fluorophore AF 488 for labeling azide-tagged biomolecules inside live cells, whole organisms, and inanimate samples.
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Cat. # Quantity Price Lead time
118F0 1 mg $110.00 in stock
218F0 5 mg $290.00 in stock
418F0 25 mg $690.00 in stock
518F0 50 mg $1270.00 in stock
618F0 100 mg $1990.00 in stock

AF 568 DBCO, 6-isomer

Dibenzocyclooctyne (DBCO, ADIBO) containing the orange fluorophore AF 568 for cooper-free labeling azide-tagged biomolecules inside live cells, whole organisms, and inanimate samples.
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Cat. # Quantity Price Lead time
1158F0 1 mg $110.00 in stock
2158F0 5 mg $290.00 in stock
4158F0 25 mg $690.00 in stock
5158F0 50 mg $1270.00 in stock
6158F0 100 mg $1990.00 in stock

AmdU (5-Azidomethyl-2'-deoxyuridine)

Azidomethyldeoxyuridine (AmdU) is an azide-contained nucleoside that is incorporated into nascent DNA by cellular polymerases. The azide groups attached to DNA this way can be subsequently modified with alkynes or cycloalkynes via copper-catalyzed or strain-promoted click reaction.
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Cat. # Quantity Price Lead time
B1540 5 mg $120.00 in stock
D1540 25 mg $255.00 in stock
F1540 100 mg $890.00 in stock

BDP 558/568 DBCO

BDP 558/568 DBCO is a bright and photostable dye with emission in the yellow spectrum range. It contains a cyclooctyne substituting group (i.e. DBCO), which can react with various azides in Click Chemistry reactions.
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Cat. # Quantity Price Lead time
174F0 1 mg $110.00 in stock
274F0 5 mg $210.00 in stock
374F0 10 mg $310.00 in stock
474F0 25 mg $410.00 in stock
574F0 50 mg $695.00 in stock
674F0 100 mg $1190.00 in stock

BDP 558/568 tetrazine

Stable fluorophore that can be conjugated with trans-cyclooctene derivatives of biomolecules for their visualization.
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Cat. # Quantity Price Lead time
A74E0 1 mg $110.00 in stock
B74E0 5 mg $210.00 in stock
C74E0 10 mg $310.00 in stock
D74E0 25 mg $410.00 in stock
E74E0 50 mg $695.00 in stock
F74E0 100 mg $1190.00 in stock

BDP 576/589 tetrazine

Tetrazine derivative of BDP 576/589 hydrophobic dye (BODIPY™ 576/589 analog) for conjugation with trans-cyclooctenes and cyclopropenes in the TCO ligation reaction.
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Cat. # Quantity Price Lead time
1G4E0 1 mg $110.00 in stock
2G4E0 5 mg $290.00 in stock
3G4E0 10 mg $450.00 in stock
4G4E0 25 mg $690.00 in stock
5G4E0 50 mg $1270.00 in stock
6G4E0 100 mg $1990.00 in stock

BDP 581/591 DBCO

BDP 581/591 is a borondipyrromethene dye that can be used not only as a fluorescent dye, but also as a ROS probe. It can be conjugated with azides using copper-free click chemistry.
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Cat. # Quantity Price Lead time
A64F0 1 mg $110.00 in stock
B64F0 5 mg $210.00 in stock
C64F0 10 mg $310.00 in stock
D64F0 25 mg $410.00 in stock
E64F0 50 mg $695.00 in stock
F64F0 100 mg $1190.00 in stock

BDP 581/591 tetrazine

BDP 581/591 is a borondipyrromethene dye with tetrazine group for the inverse electron demand Diels-Alder (IEDDA) ligation.
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Cat. # Quantity Price Lead time
164E0 1 mg $110.00 in stock
264E0 5 mg $290.00 in stock
464E0 25 mg $690.00 in stock
564E0 50 mg $1270.00 in stock
664E0 100 mg $1990.00 in stock

BDP 630/650 tetrazine

BDP 630/650 is a borondipyrromethene dye for Cyanine5 channel. This is a tetrazine derivative for the coupling with strained cycloalkenes.
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Cat. # Quantity Price Lead time
154E0 1 mg $110.00 in stock
254E0 5 mg $290.00 in stock
454E0 25 mg $690.00 in stock
554E0 50 mg $1270.00 in stock
654E0 100 mg $1990.00 in stock

BDP 650/665 DBCO

This red-fluorescent dye contains a substituting group for copper-free click chemistry.
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Cat. # Quantity Price Lead time
AE4F0 1 mg $110.00 in stock
BE4F0 5 mg $210.00 in stock
CE4F0 10 mg $310.00 in stock
DE4F0 25 mg $410.00 in stock
EE4F0 50 mg $695.00 in stock
FE4F0 100 mg $1190.00 in stock

BDP FL DBCO

BDP FL DBCO is a dye cyclooctyne for copper-free click chemistry reaction with azides. Absorption and emission wavelengths of BDP FL correspond to those of fluorescein (FAM), but BDP FL is significantly more photostable.
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Cat. # Quantity Price Lead time
A14F0 1 mg $110.00 in stock
B14F0 5 mg $210.00 in stock
C14F0 10 mg $310.00 in stock
D14F0 25 mg $410.00 in stock
E14F0 50 mg $695.00 in stock
F14F0 100 mg $1190.00 in stock

BDP FL tetrazine

BDP FL tetrazine (methyltetrazine) is a reactive dye for the inverse electron demand Diels-Alder (ied-DA) reaction with trans-cyclooctenes, cyclopropenes, and other strained cycloolefines. BDP FL is a bright and photostable dye for the 488 nm fluorescein (FAM) channel.
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Cat. # Quantity Price Lead time
114E0 1 mg $110.00 in stock
214E0 5 mg $290.00 in stock
414E0 25 mg $690.00 in stock
514E0 50 mg $1270.00 in stock
614E0 100 mg $1990.00 in stock

BDP R6G DBCO

A derivative of BDP R6G dye with azodibenzocyclooctyne (DBCO or ADIBO) for copper-free click chemistry conjugation with azides.
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Cat. # Quantity Price Lead time
A44F0 1 mg $110.00 in stock
B44F0 5 mg $210.00 in stock
C44F0 10 mg $310.00 in stock
D44F0 25 mg $410.00 in stock
E44F0 50 mg $695.00 in stock
F44F0 100 mg $1190.00 in stock

BDP R6G tetrazine

BDP R6G tetrazine is a borondipyrromethene dye with absorption and emission wavelengths similar to rhodamine 6G. It contains tetrazine fragment for IEDDA ligation.
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Cat. # Quantity Price Lead time
144E0 1 mg $110.00 in stock
244E0 5 mg $290.00 in stock
444E0 25 mg $690.00 in stock
544E0 50 mg $1270.00 in stock
644E0 100 mg $1990.00 in stock

BDP TMR DBCO

This yellow-orange-fluorescent boron dipyrromethene dye contains a DBCO substituting group for participation in click chemistry reactions.
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Cat. # Quantity Price Lead time
124F0 1 mg $110.00 in stock
224F0 5 mg $210.00 in stock
424F0 25 mg $410.00 in stock
524F0 50 mg $695.00 in stock
624F0 100 mg $1190.00 in stock

BDP TMR tetrazine

A fluorophore for the TAMRA channel with a functional group that can form conjugates in click chemistry reactions (IEDDA ligation) .
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Cat. # Quantity Price Lead time
124E0 1 mg $110.00 in stock
224E0 5 mg $210.00 in stock
424E0 25 mg $410.00 in stock
524E0 50 mg $695.00 in stock
624E0 100 mg $1190.00 in stock

BDP TR DBCO

A dye with a dibenzocyclooctyne substituting group for the ROX hannel.
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Cat. # Quantity Price Lead time
1D4F0 1 mg $110.00 in stock
2D4F0 5 mg $210.00 in stock
4D4F0 25 mg $410.00 in stock
5D4F0 50 mg $695.00 in stock
6D4F0 100 mg $1190.00 in stock

BDP TR tetrazine

BDP TR is a borondipyrromethene fluorophore for ROX channel. The dye produces bright fluorescent emission. Tetrazines are reactive components for the conjugation with strained cycloalkenes.
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Cat. # Quantity Price Lead time
134E0 1 mg $110.00 in stock
234E0 5 mg $290.00 in stock
434E0 25 mg $690.00 in stock
534E0 50 mg $1270.00 in stock
634E0 100 mg $1990.00 in stock