endo-BCN CE-phosphoramidite
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
3005-100mg | 100 mg | $390 | in stock | |
3005-250mg | 250 mg | $850 | in stock | |
3005-1g | 1 g | $2380 | in stock |
Bicyclononyne (BCN) is a stable and one of the most reactive cyclooctynes for copper-free click chemistry. Unlike dibenzocyclooctyne (DBCO), BCN is reactive both to azides (strain-promoted azyde-alkyne cycloaddition, SPAAC) and tetrazines (inverse electron demand Diels-Alder reaction, IEDDA). Being an endo-stereoisomer, bicyclononine in endo-BCN CE-phosphoramidite provides not significantly different rate of cycloaddition compared to its exo-conformer.
BCN-labeled oligonucleotides may be used for the conjugation to azide- or tetrazine-containing solid surfaces, polymers, and large proteins.
Coupling time is standard, like for amidites of natural nucleosides. Exclude the dimethoxytrityl (DMT) removal step and use the Dmt-ON protocol after amidite coupling and oxidation.
Use standard conditions for deprotection and ammonia solution, or AMA mixture (ammonium hydroxide / 40% methylamine, 1:1).
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Appearance: | yellowish oil |
Mass spec M+ increment: | 343.11 |
Molecular weight: | 481.57 |
CAS number: | 1352811-59-6 |
Molecular formula: | C24H40F1N3O5P |
Solubility: | good in acetonitrile |
Quality control: | NMR 1H and 31P (95 %) |
Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
MSDS: | Download |
Product specifications |
Oligo synthesis details
Diluent: | Anhydrous Acetonitrile |
Deprotection conditions: | identical to protected nucleobases |