Cyanine7.5 azide

Cat. # Quantity Price Lead time
46030 1 mL, 10 mM in DMSO $410.00 in stock
16030 100 uL, 10 mM/DMSO $110.00 in stock
36030 500 uL, 10 mM/DMSO $210.00 in stock
A6030 1 mg $110.00 in stock
B6030 5 mg $210.00 10 days
C6030 10 mg $310.00 in stock
D6030 25 mg $410.00 in stock
E6030 50 mg $695.00 in stock
F6030 100 mg $1190.00 in stock

Cyanine7.5 is a NIR dye with long-wave infrared fluorescence. This derivative is azide for Click Chemistry.

Can be used for the construction of various labeled biomolecules containing Cyanine7.5, near infrared fluorescent dye, and an improved analog of Cy7.5®. These conjugates can take advantage of NIR tissue transparency when used for in vivo imaging. This fluorophore is also useful for other fluorescent applications, especially requiring low fluorescent background.

Azide is available as DMSO solution, ready for general Click Chemistry labeling protocol, or in solid form for custom labeling applications.

Structure features rigid bridged polymethyne chain to increase quantum yield by 20%, allowing for brighter signal.

Cyanine7.5 absorbance and emission spectra

Cyanine7.5 absorbance and emission spectra

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FAM azide, 5-isomer

FAM (fluorescein) azide for Click chemistry, pure 5-isomer.

General properties

Appearance: green powder / solution
Molecular weight: 767.44
Molecular formula: C48H55ClN6O
Solubility: soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 788
ε, L⋅mol−1⋅cm−1: 223000
Emission maximum, nm: 808

Product citations

  1. Retout, M.; Brunetti, E.; Valkenier, H.; Bruylants, G. Limits of thiol chemistry revealed by quantitative analysis of mixed layers of thiolated-PEG ligands grafted onto gold nanoparticles. Journal of Colloid and Interface Science, 2019, 557, 807–815. doi: 10.1016/j.jcis.2019.09.047
  2. Pearce, H.A.; Qian, H.; Connell, T.U.; Huang, D.; Gottstein, C.; Donnelly, P.S.; Peter, K.; Gregorevic, P.; Hagemeyer, C.E. Site-specific glycation and chemo-enzymatic antibody sortagging for the retargeting of rAAV6 to inflamed endothelium. Molecular Therapy - Methods & Clinical Development, 2019, 14, 261–269. doi: 10.1016/j.omtm.2019.07.003
  3. Kong, J.-N.; Zhu, Z.; Itokazu, Y.; Wang, G.; Dinkins, M.B.; Zhong, L.; Lin, H.-P.; Elsherbini, A.; Leanhart, S.; Jiang, X.; Qin, H.; Zhi, W.; Spassieva, S.D.; Bieberich, E. Novel function of ceramide for regulation of mitochondrial ATP release in astrocytes. Journal of Lipid Research, 2018, 59(3), 488–506. doi: 10.1194/jlr.M081877
  4. van der Steen, S.C.H.A.; Raavé, R.; Langerak, S.; van Houdt, L.; van Duijnhoven, S.M.J.; van Lith, S.A.M.; Massuger, L.F.A.G.; Daamen, W.F.; Leenders, W.P.; van Kuppevelt, T.H. Targeting the extracellular matrix of ovarian cancer using functionalized, drug loaded lyophilisomes. European Journal of Pharmaceutics and Biopharmaceutics, 2017, 113, 229–239. doi: 10.1016/j.ejpb.2016.12.010
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