Cyanine3.5 NHS ester

Cat. # Quantity Price Lead time
12020 1 mg $110.00 in stock
22020 5 mg $210.00 in stock
42020 25 mg $410.00 5 days
52020 50 mg $695.00 in stock
62020 100 mg $1190.00 in stock

Cyanine3.5 NHS ester (an analog of Cy3.5® NHS ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides.

Cyanine3.5 NHS ester can replace NHS esters of Cy3.5®, Alexa Fluor 594, DyLight 594.

Cyanine3.5 NHS ester absorbance and emission spectra

Cyanine3.5 NHS ester absorbance and emission spectra

Customers also purchased with this product

Coumarin 343 X azide

Coumarin 343 is a blue emitting fluorophore that can serve as a FRET donor for fluorescein (FAM). This azide derivative can be conjugated with alkynes by Click chemistry. An extended linker arm between the fluorophore and the azide group enhances solubility, and provides spatial separation between the dye and the biomolecule.

Sulfo-Cyanine3 azide

Water-soluble sulfo-Cyanine3 dye azide for Click Chemistry.

Sulfo-Cyanine5.5 NHS ester

Water soluble, sulfonated, far-red sulfo-Cyanine5.5 dye in the form of NHS ester.

General properties

Appearance: dark purple solid
Molecular weight: 741.62
CAS number: 2231670-85-0
Molecular formula: C42H44N3BF4O4
Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water
Quality control: NMR 1H and HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 591
ε, L⋅mol−1⋅cm−1: 116000
Emission maximum, nm: 604
Fluorescence quantum yield: 0.35
CF260: 0.29
CF280: 0.22

Product citations

  1. Lee, S.A.; Biteen, J.S. Spectral Reshaping of Single Dye Molecules Coupled to Single Plasmonic Nanoparticles. Journal of Physical Chemistry Letters, 2019, 10, 5764–5769. doi: 10.1021/acs.jpclett.9b02480
  2. Kravchuk, O.I.; Lyupina, Y.V.; Erokhov, P.A.; Finoshin, A.D.; Adameyko, K.I.; Mishyna, M.Y.; Moiseenko, A.V.; Sokolova, O.S.; Orlova, O.V.; Beljelarskaya, S.N.; Serebryakova, M.V.; Indeykina, M.I.; Bugrova, A.E.; Kononikhin, A.S.; Mikhailov, V.S. Characterization of the 20S proteasome of the lepidopteran, Spodoptera frugiperda. Biochimica et Biophysica Acta - Proteins and Proteomics, 2019, 1867(9), 840–853. doi: 10.1016/j.bbapap.2019.06.010
  3. Wang, C.; Niederstrasser, H.; Douglas, P.M.; Lin, R.; Jaramillo, J.; Li, Y.; Olswald, N.W.; Zhou, A.; McMillan, E.A.; Mendiratta, S.; Wang, Z.; Zhao, T.; Lin, Z.; Luo, M.; Huang, G.; Brekken, R.A.; Posner, B.A.; MacMillan, J.B.; Gao, J.; White, M.A. Small-molecule TFEB pathway agonists that ameliorate metabolic syndrome in mice and extend C. elegans lifespan. Nature Communications, 2017, 8, 2270. doi: 10.1038/s41467-017-02332-3
  4. Massey, M.; Kim, H.; Conroy, E.M.; Algar, W.R. Expanded Quantum Dot-Based Concentric Förster Resonance Energy Transfer: Adding and Characterizing Energy-Transfer Pathways for Triply Multiplexed Biosensing. The Journal of Physical Chemistry C, 2017, 121(24), 13345–13356. doi: 10.1021/acs.jpcc.7b02739
Show more (2)
Your item has been added. View your cart or proceed to checkout
The count of items is incorrect.