Cyanine5.5 alkyne
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A70B0 | 1 mg | $110.00 | in stock | |
| B70B0 | 5 mg | $210.00 | in stock | |
| C70B0 | 10 mg | $310.00 | in stock | |
| D70B0 | 25 mg |
$410.00
|
in stock | |
| E70B0 | 50 mg |
$695.00
|
in stock | |
| F70B0 | 100 mg |
$1190.00
|
in stock |
Far red / near infrared dye alkyne for Click Chemistry labeling. Cyanine5.5 is an analog of Cy5.5®, a popular fluorophore which has been widely used for various applications including intact organism imaging. This reagent can be conjugated with azido groups under mild copper catalyzed Click Chemistry conditions.
This reagent is soluble in organic solvents, but mixtures of water with small percent of DMSO can be used for efficient conjugation. Cyanine5.5 alkyne can also be used for the labeling of small molecules with this far red/NIR dye.
Cyanine5.5 absorbance and emission spectra
Recommended protocol
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Cyanine5.5 hydrazide is a carbonyl reactive NIR dye which is particularly suitable for the labeling of periodate oxidized glycoproteins, i.e. antibodies.Cyanine3.5 NHS ester
Cyanine3.5 dye NHS ester derivative.
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General properties
| Appearance: | dark blue powder |
| Molecular weight: | 656.30 |
| Molecular formula: | C43H46ClN3O |
| Solubility: | good in organic solvents (DMF, DMSO, acetonitrile, DCM, alcohols), practically insoluble in water (5.2 uM, 3.7 mg/L) |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 684 |
| ε, L⋅mol−1⋅cm−1: | 198000 |
| Emission maximum, nm: | 710 |
| Fluorescence quantum yield: | 0.2 |
| CF260: | 0.07 |
| CF280: | 0.03 |
Product citations
- Mickuté, M.; Nainyté, M.; Vasiliauskaité, L.; Plotnikova, A.; Masevičius, V.; Klimašauskas, S.; Vilkaitis, G. Animal Hen1 2'-O-methyltransferases as tools for 3'-terminal functionalization and labelling of single-stranded RNAs. Nucleic Acids Research, 2018, 46(17), e104. doi: 10.1093/nar/gky514
- Yuan, H.; Wilks, M.Q.; Fakhri, G.E.; Normandin, M.D.; Kaittanis, C.; Josephson, L. Heat-induced-radiolabeling and click chemistry: A powerful combination for generating multifunctional nanomaterials. PLoS One, 2017, 12(2), e0172722. doi: 10.1371/journal.pone.0172722
- Duan, Z.; Zhang, Y.; Zhu, H.; Sun, L.; Cai, H.; Li, B.; Gong, Q.; Gu, Z.; Luo, K. Stimuli-Sensitive Biodegradable and Amphiphilic Block Copolymer-Gemcitabine Conjugates Self-assemble Into a Nanoscale Vehicle for Cancer Therapy. ACS Applied Materials & Interfaces, 2017, 9(4), 3474–3486. doi: 10.1021/acsami.6b15232
- Shi, Y.; van der Meel, R.; Theek, B.; Oude Blenke, E.; Pieters, E.H.E.; Fens, M.H.A.M.; Ehling, J.; Schiffelers, R.M.; Storm, G.; van Nostrum, C.F.; Lammers, T.; Hennink, W.E. Complete Regression of Xenograft Tumors upon Targeted Delivery of Paclitaxel via Π–Π Stacking Stabilized Polymeric Micelles. ACS Nano, 2015, 9(4), 3740–3752. doi: 10.1021/acsnano.5b00929
Cy® is a trademark of GE Healthcare.
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