Sulfo-Cyanine3 NHS ester

Cat. # Quantity Price Lead time
11320 1 mg $110.00 in stock
21320 5 mg $290.00 in stock
41320 25 mg $690.00 in stock
51320 50 mg $1270.00 in stock
61320 100 mg $1990.00 in stock

Water soluble, amino-reactive sulfo-Cyanine3 NHS ester. Efficiently labels proteins and peptides in purely aqueous solution, without need for organic co-solvent. Ideal for proteins with low solubility, and proteins prone to denaturation.

This is sulfonated, hydrophilic and water-soluble dye. Non-sulfonated Cyanine3 NHS ester is also available.

This product is an analog of Cy3® NHS ester. Sulfo-Cyanine3 NHS ester replaces Cy3®, Alexa Fluor 546, and DyLight 549 for all applications.

Sulfo-Cyanine3 absorbance and emission spectra

Sulfo-Cyanine3 absorbance and emission spectra

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General properties

Appearance: dark red crystals
Molecular weight: 751.91
CAS number: 1424150-38-8 (sodium salt); 1424433-17-9, 1518643-34-9 (inner salt)
Molecular formula: C34H38N3KO10S2
IUPAC name: 3H-​Indolium, 2-​[3-​(1,​3-​dihydro-​1,​3,​3-​trimethyl-​5-​sulfo-​2H-​indol-​2-​ylidene)​-​1-​propen-​1-​yl]​-​1-​[6-​[(2,​5-​dioxo-​1-​pyrrolidinyl)​oxy]​-​6-​oxohexyl]​-​3,​3-​dimethyl-​5-​sulfo-​, inner salt, sodium salt
Solubility: soluble in water (0.62 M = 47 g/L), in polar organic solvents (DMF, DMSO)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 548
ε, L⋅mol−1⋅cm−1: 162000
Emission maximum, nm: 563
Fluorescence quantum yield: 0.1
CF260: 0.03
CF280: 0.06

Product citations

  1. Tesarova, B.; Dostalova, S.; Smidova, V.; Goliasova, Z.; Skubalova, Z.; Michalkova, H.; Hynek, D.; Michalek, P.; Polanska, H.; Vaculovicova, M.; Hacek, J.; Eckschlager, T.; Stiborova, M.; Pires, A.S.; Neves, A.R.M.; Abrantes, A.M.; Rodrigues, T.; Matafome, P.; Botelho, M.F.; Teixeira, P.; Mendes, F.; Heger, Z. Surface-PASylation of ferritin to form stealth nanovehicles enhances in vivo therapeutic performance of encapsulated ellipticine. Applied Materials Today, in press. doi: 10.1016/j.apmt.2019.100501
  2. Taghian, T.; Metelev, V.G.; Zhang, S.; Bogdanov, A.A. Imaging NF-κB activity in a murine model of early stage diabetes. FASEB Journal, in press. doi: 10.1096/fj.201801147r
  3. Umakoshi, T.; Fukuda, S.; Iino, R.; Uchihashi, T.; Ando, T. High-speed near-field fluorescence microscopy combined with high-speed atomic force microscopy for biological studies. Biochimica et Biophysica Acta, 2020, 1864(2), 129325. doi: 10.1016/j.bbagen.2019.03.011
  4. Antonov, S.A.; Novosadova, E.V.; Kobylansky, A.G.; Tarantul, V.Z.; Grivennikov, I.A. A Hybrid Detection Method Based on Peroxidase-mediated Signal Amplification and Click Chemistry for Highly Sensitive Background-free Immunofluorescent Staining. Journal of Histochemistry & Cytochemistry, 2019, 67(10), 771–782. doi: 10.1369/0022155419864113
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