Sulfo-Cyanine3 NHS ester
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11320 | 1 mg | $110.00 | in stock | |
| 21320 | 5 mg | $210.00 | in stock | |
| 41320 | 25 mg |
$410.00
|
in stock | |
| 51320 | 50 mg |
$695.00
|
in stock | |
| 61320 | 100 mg |
$1190.00
|
in stock |
Water soluble, amino-reactive sulfo-Cyanine3 NHS ester. Efficiently labels proteins and peptides in purely aqueous solution, without need for organic co-solvent. Ideal for proteins with low solubility, and proteins prone to denaturation.
This is sulfonated, hydrophilic and water-soluble dye. Non-sulfonated Cyanine3 NHS ester is also available.
This product is an analog of Cy3® NHS ester. Sulfo-Cyanine3 NHS ester replaces Cy3®, Alexa Fluor 546, and DyLight 549 for all applications.
Sulfo-Cyanine3 absorbance and emission spectra
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General properties
| Appearance: | dark red crystals |
| Molecular weight: | 751.91 |
| CAS number: | 1424150-38-8 (sodium salt); 1424433-17-9, 1518643-34-9 (inner salt) |
| Molecular formula: | C34H38N3KO10S2 |
| IUPAC name: | 3H-Indolium, 2-[3-(1,3-dihydro-1,3,3-trimethyl-5-sulfo-2H-indol-2-ylidene)-1-propen-1-yl]-1-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-3,3-dimethyl-5-sulfo-, inner salt, sodium salt |
| Solubility: | soluble in water (0.62 M = 47 g/L), in polar organic solvents (DMF, DMSO) |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 548 |
| ε, L⋅mol−1⋅cm−1: | 162000 |
| Emission maximum, nm: | 563 |
| Fluorescence quantum yield: | 0.1 |
| CF260: | 0.03 |
| CF280: | 0.06 |
Product citations
- Lee, M.S.; Hyun, H.; Park, I.; Kim, S.; Jang, D.-H.; Kim, S.; Im, J.-K.; Kim, H.; Lee, J.H.; Kwon, T.; Kang, J.H. Quantitative fluorescence in situ hybridization (FISH) of magnetically confined bacteria enables rapid determination of early-stage human bacteremia. medRxiv, preprint. doi: 10.1101/2021.02.19.21251962
- Baker, Y.; Yuan, L.; Chen J.; Belle, R.; Carlisle, R.; El-Sagheer, A.; Brown, T. Expanding the Chemical Repertoire of DNA Nanomaterials Generated by Rolling Circle Amplification. ChemRxiv, preprint. doi: 10.26434/chemrxiv.12566963.v1
- Liu, Z.; Yuan, J.; Lasorella, A.; Iavarone, A.; Bruce, J.N.; Canoll, P.; Sims, P.A. Integrating single-cell RNA-seq and imaging with SCOPE-seq2. Scientific Reports, 2020, 10, 19482. doi: 10.1038/s41598-020-76599-w
- Binkley, M.; Cui, M.; Berezin, M.; Meacham, J.M. Antibody conjugate assembly on ultrasound-confined microcarrier particles. ACS Biomaterials Science & Engineering, 2020, 6(11), 6108–6116. doi: 10.1021/acsbiomaterials.0c01162
Cy® is a trademark of GE Healthcare.
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