Sulfo-Cyanine3 NHS ester
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11320 | 1 mg | $110.00 | in stock | |
| 21320 | 5 mg | $290.00 | in stock | |
| 41320 | 25 mg |
$690.00
|
in stock | |
| 51320 | 50 mg |
$1270.00
|
in stock | |
| 61320 | 100 mg |
$1990.00
|
in stock |
Water soluble, amino-reactive sulfo-Cyanine3 NHS ester. Efficiently labels proteins and peptides in purely aqueous solution, without need for organic co-solvent. Ideal for proteins with low solubility, and proteins prone to denaturation.
This is sulfonated, hydrophilic and water-soluble dye. Non-sulfonated Cyanine3 NHS ester is also available.
This product is an analog of Cy3® NHS ester. Sulfo-Cyanine3 NHS ester replaces Cy3®, Alexa Fluor 546, and DyLight 549 for all applications.
Sulfo-Cyanine3 absorbance and emission spectra
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Sulfo-Cyanine3 amine
Water soluble cyanine dye with bright fluorescence, and high extinction coefficient. Contains an amino group for the conjugation with electrophiles, and enzymatic transamination labeling.General properties
| Appearance: | dark red crystals |
| Molecular weight: | 751.91 |
| CAS number: | 1424150-38-8 (sodium salt); 1424433-17-9, 1518643-34-9 (inner salt) |
| Molecular formula: | C34H38N3KO10S2 |
| IUPAC name: | 3H-Indolium, 2-[3-(1,3-dihydro-1,3,3-trimethyl-5-sulfo-2H-indol-2-ylidene)-1-propen-1-yl]-1-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-3,3-dimethyl-5-sulfo-, inner salt, sodium salt |
| Solubility: | soluble in water (0.62 M = 47 g/L), in polar organic solvents (DMF, DMSO) |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 548 |
| ε, L⋅mol−1⋅cm−1: | 162000 |
| Emission maximum, nm: | 563 |
| Fluorescence quantum yield: | 0.1 |
| CF260: | 0.03 |
| CF280: | 0.06 |
Product citations
- Choi, J.; Marks, J.; Zhang, J.; Chen, D.-H.; Wang, J.; Vázquez-Laslop, N.; Mankin, A.S:; Puglisi, J.D. Dynamics of the context-specific translation arrest by chloramphenicol and linezolid. Nature Chemical Biology, 2020, 16, 310–317. doi: 10.1038/s41589-019-0423-2
- Song, P.; Shen, J.; Ye, D.; Dong, B.; Wang, F.; Pei, H.; Wang, J.; Shi, J.; Wang, L.; Xue, W.; Huang, Y.; Huang, G.; Zuo, X.; Fan, C. Programming bulk enzyme heterojunctions for biosensor development with tetrahedral DNA framework. Nature Communications, 2020, 11, 838. doi: 10.1038/s41467-020-14664-8
- Tesarova, B.; Dostalova, S.; Smidova, V.; Goliasova, Z.; Skubalova, Z.; Michalkova, H.; Hynek, D.; Michalek, P.; Polanska, H.; Vaculovicova, M.; Hacek, J.; Eckschlager, T.; Stiborova, M.; Pires, A.S.; Neves, A.R.M.; Abrantes, A.M.; Rodrigues, T.; Matafome, P.; Botelho, M.F.; Teixeira, P.; Mendes, F.; Heger, Z. Surface-PASylation of ferritin to form stealth nanovehicles enhances in vivo therapeutic performance of encapsulated ellipticine. Applied Materials Today, 2020, 18, 100501. doi: 10.1016/j.apmt.2019.100501
- Taghian, T.; Metelev, V.G.; Zhang, S.; Bogdanov, A.A. Imaging NF-κB activity in a murine model of early stage diabetes. FASEB Journal, 2020, 34(1), 1198–1210. doi: 10.1096/fj.201801147r
Cy® is a trademark of GE Healthcare.
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