sulfo-Cyanine3 NHS ester

Cat. # Quantity Price Lead time
11320 1 mg $91.00 in stock
21320 5 mg $240.00 in stock
41320 25 mg $695.00 in stock
51320 50 mg $850.00 in stock
61320 100 mg $1380.00 in stock

Water soluble, amino-reactive sulfo-Cyanine3 NHS ester that efficiently labels proteins and peptides in purely aqueous solution without needing organic co-solvent. Ideal for proteins with low solubility and proteins prone to denaturation.

This is the sulfonated, hydrophilic, and water-soluble dye. Non-sulfonated Cyanine3 NHS ester is also available.

This product is an analog of Cy3® NHS ester. sulfo-Cyanine3 NHS ester replaces Cy3® and DyLight 549 for all applications.

Sulfo-Cyanine3 absorbance and emission spectra

Sulfo-Cyanine3 absorbance and emission spectra

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sulfo-Cyanine7 bis-NHS ester

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General properties

Appearance: dark red crystals
Molecular weight: 751.91
CAS number: 1424150-38-8 (sodium salt); 1424433-17-9, 1518643-34-9 (inner salt)
Molecular formula: C34H38N3KO10S2
IUPAC name: 3H-​Indolium, 2-​[3-​(1,​3-​dihydro-​1,​3,​3-​trimethyl-​5-​sulfo-​2H-​indol-​2-​ylidene)​-​1-​propen-​1-​yl]​-​1-​[6-​[(2,​5-​dioxo-​1-​pyrrolidinyl)​oxy]​-​6-​oxohexyl]​-​3,​3-​dimethyl-​5-​sulfo-​, inner salt, sodium salt
Solubility: soluble in water (0.62 M = 47 g/L), in polar organic solvents (DMF, DMSO)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 548
ε, L⋅mol−1⋅cm−1: 162000
Emission maximum, nm: 563
Fluorescence quantum yield: 0.1
CF260: 0.03
CF280: 0.06

Product citations

  1. McGregor, L. A.; Zhu, B.; Goetz, A. M.; Sczepanski, J. T. Thymine DNA Glycosylase Is an RNA-Binding Protein with High Selectivity for G-Rich Sequences. Journal of Biological Chemistry, 2023, 299(4), 104590. doi: 10.1016/j.jbc.2023.104590
  2. Breine, A.; Van holsbeeck, K.; Martin, C.; Gonzalez, S.; Mannes, M.; Pardon, E.; Steyaert, J.; Remaut, H.; Ballet, S.; Van der Henst, C. Bypassing the Need for Cell Permeabilization: Nanobody CDR3 Peptide Improves Binding on Living Bacteria. Bioconjugate Chem, 2023, 34(7), 1234–1243. doi: 10.1021/acs.bioconjchem.3c00116
  3. Grohme, M.; Frank, O.; Rink, J. Preparing Planarian Cells for High-content Fluorescence Microscopy Using RNA in situ Hybridization and Immunocytochemistry. Methods in Molecular Biology, 2023, 2680, 121-155. doi: 10.1007/978-1-0716-3275-8_8
  4. Petit, P.-F.; Bombart, R.; Desimpel, P.-H.; Naulaerts, S.; Thouvenel, L.; Collet, J.-F.; Van Den Eynde, B. J.; Zhu, J. T Cell–Mediated Targeted Delivery of Anti–PD-L1 Nanobody Overcomes Poor Antibody Penetration and Improves PD-L1 Blocking at the Tumor Site. Cancer Immunology Research, 2022, 10(5), 713–727. doi: 10.1158/2326-6066.CIR-21-0801
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