Sulfo-Cyanine3 NHS ester

sulfo-Cyanine3 NHS esters Microscopy
Cat. # Quantity Price Lead time
11320 1 mg $110.00 in stock
21320 5 mg $290.00 in stock
41320 25 mg $690.00 in stock
51320 50 mg $1270.00 5 days
61320 100 mg $1990.00 in stock
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Water soluble, amino-reactive sulfo-Cyanine3 NHS ester. Efficiently labels proteins and peptides in purely aqueous solution, without need for organic co-solvent. Ideal for proteins with low solubility, and proteins prone to denaturation.

This is sulfonated, hydrophilic and water-soluble dye. Non-sulfonated Cyanine3 NHS ester is also available.

This product is an analog of Cy3® NHS ester. Sulfo-Cyanine3 NHS ester replaces Cy3®, Alexa Fluor 546, and DyLight 549 for all applications.

Sulfo-Cyanine3 NHS ester
Sulfo-Cyanine3 NHS ester
Sulfo-Cyanine3 NHS ester
Water soluble sulfo-Cy3 NHS ester structure
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Sulfo-Cyanine3 absorbance and emission spectra

Sulfo-Cyanine3 absorbance and emission spectra

Recommended protocol

NHS ester labeling of amino biomolecules

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General properties

Appearance: dark red crystals
Molecular weight: 735.80
Molecular formula: C34H38N3NaO10S2
Solubility: very high in water, good in polar organic solvents (DMF, DMSO)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download

Spectral properties

Excitation maximum, nm: 548
ε, L⋅mol−1⋅cm−1: 162000
Emission maximum, nm: 563
Fluorescence quantum yield: 0.1
CF260: 0.03
CF280: 0.06

Product citations

  1. Shi, X.; Yu, C.Y.Y.; Su, H.; Kwok, R.T.K.; Jiang, M.; He, Z.; Lam, J.W.Y.; Tang, B.Z. A Red-emissive antibody–AIEgen conjugate for turn-on and wash-free imaging of specific cancer cells. Chemical Science, in press. doi: 10.1039/C7SC01054K
  2. Zabara, A.; Meikle, T.G.; Trenker, R.; Yao, S.; Newman, J.; Peat, T.S.; Separovic, F.; Conn, C.E.; Call, M.J.; Call, M.E.; Landau, E.M.; Drummond, C.J. Lipidic Cubic Phase-Induced Membrane Protein Crystallization: Interplay Between Lipid Structure, Mesophase Properties, and Crystallogenesis. Crystal Growth and Design, in press. doi: 10.1021/acs.cgd.7b00519
  3. Schneider, J.R.; Carias, A.M.; Bastian, A.R.; Cianci, G.C.; Kiser, P.F.; Veazey, R.S.; Hope, T.J. Long-term direct visualization of passively transferred fluorophore-conjugated antibodies. Journal of Immunological Methods, 2017, 450, 66–72. doi: 10.1016/j.jim.2017.07.009
  4. Huang, T.-Y.; Chang, C.-K.; Kao, Y.-F.; Chin, C.-H.; Ni, C.-W.; Hsu, H.-Y.; Hu, N.-J.; Hsieh, L.-C.; Chou, S.-H.; Lee, I.-R.; Hou, M.-H. Parity-dependent hairpin configurations of repetitive DNA sequence promote slippage associated with DNA expansion. Proceedings of the National Academy of Sciences of the USA, 2017, 114(36), 9535–9540. doi: 10.1073/pnas.1708691114
  5. Barnett, D.; Liu, Y.; Partyka, K.; Huang, Y.; Tang, H.; Hostetter, G.; Brand, R.E.; Singhi, A.D.; Drake, R.R.; Haab, B.B. The CA19-9 and Sialyl-TRA Antigens Define Separate Subpopulations of Pancreatic Cancer Cells. Scientific Reports, 2017, 7, 4020. doi: 10.1038/s41598-017-04164-z
  6. Korkmaz, E.; Friedrich, E.E.; Ramadan, M.H.; Erdos, G.; Mathers, A.R.; Ozdoganlar, O.B.; Washburn, N.R.; Falo, Jr, L.D. Tip-Loaded Dissolvable Microneedle Arrays Effectively Deliver Polymer-Conjugated Antibody Inhibitors of Tumor-Necrosis-Factor-Alpha Into Human Skin. Journal of Pharmaceutical Sciences, 2016, 105(11), 3453–3457. doi: 10.1016/j.xphs.2016.07.008
  7. Zhou, L.; Wang, K.; Wu, Z.; Dong, H.; Sun, H.; Cheng, X.; Zhang, H.; Zhou, H.; Jia, C.; Jin, Q.; Mao, H.; Coll, J.-L.; Zhao, J. Investigation of Controllable Nanoscale Heat-Denatured Bovine Serum Albumin Films on Graphene. Langmuir, 2016, 32(48), 12623–12631. doi: 10.1021/acs.langmuir.6b03296
  8. Zhang, X.; MIn, L.; Jia, C. Application of Wavelet Transform Algorithm and Rolling Ball Algorithm in Single Molecule Fluorescence Resonance Energy Transfer Images. Progress in Biochemistry and Biophysics, 2016, 43(10), 997–1003. doi: 10.16476/j.pibb.2016.0218
  9. Søndergaard, S.; Aznauryan, M.; Haustrup, E.K.; Schiøtt, B.; Birkedal, V.; Corry, B. Dynamics of Fluorescent Dyes Attached to G-Quadruplex DNA and their Effect on FRET Experiments. ChemPhysChem, 2015, 16(12), 2562–2570. doi: 10.1002/cphc.201500271
  10. Wang, W.; Kapur, A.; Ji, X.; Safi, M.; Palui, G.; Palomo, V.; Dawson, P.E.; Mattoussi, H. Photoligation of an Amphiphilic Polymer with Mixed Coordination Provides Compact and Reactive Quantum Dots. Journal of the American Chemical Society, 2015, 137(16), 5438–5451. doi: 10.1021/jacs.5b00671
  11. Reid, D.A.; Keegan, S.; Leo-Macias, A.; Watanabe, G.; Strande, N.T.; Chang, H.H.; Oksuz, B.A.; Fenyo, D.; Lieber, M.R.; Ramsden, D.A.; Rothenberg, E. Organization and dynamics of the nonhomologous end-joining machinery during DNA double-strand break repair. Proceedings of the National Academy of Sciences of the U.S.A., 2015, 112(20), E2575–E2584. doi: 10.1073/pnas.1420115112
  12. Glembockyte, V.; Lincoln, R.; Cosa, G. Cy3 Photoprotection Mediated by Ni2+ for Extended Single-Molecule Imaging: Old Tricks for New Techniques. Journal of the American Chemical Society, 2015, 137(3), 1116–1122. doi: 10.1021/ja509923e
  13. Martell, D.J.; Joshi, C.P.; Gaballa, A.; Santiago, A.G.; Chen, T.-Y.; Jung, W.; Helmann, J.D.; Chen, P. Metalloregulator CueR biases RNA polymerase's kinetic sampling of dead-end or open complex to repress or activate transcription. Proceedings of the National Academy of Sciences of the U.S.A., 2015, 112(44), 13467–13472. doi: 10.1073/pnas.1515231112
  14. Graen, T.M.D.; Hoefling, M.; Grubmüller, H. AMBER-DYES: Characterization of Charge Fluctuations and Force Field Parameterization of Fluorescent Dyes for Molecular Dynamics Simulations. Journal of Chemical Theory and Computation, 2014, 10(12), 5505-5512. doi: 10.1021/ct500869p
  15. Wang, W.; Ji, X.; Na, H.B.; Safi, M.; Smith, A.; Palui, G.; Perez, J.M.; Mattoussi, H. Design of a Multi-Dopamine-Modified Polymer Ligand Optimally Suited for Interfacing Magnetic Nanoparticles with Biological Systems. Langmuir, 2014, 30(21), 6197-6208. doi: 10.1021/la500974r
  16. Aldeek, F.; Muhammed, M.A.H.; Palui, G.; Zhan, N.; Mattoussi, H. Growth of Highly Fluorescent Polyethylene Glycol- and Zwitterion-Functionalized Gold Nanoclusters. ACS Nano, 2013, 7(3), 2509-2521. doi: 10.1021/nn305856t
  17. Zhan, N.; Palui, G.; Safi, M.; Ji, X.; Mattoussi, H. Multidentate Zwitterionic Ligands Provide Compact and Highly Biocompatible Quantum Dots. Journal of the American Chemical Society, 2013, 135(37), 13786-13795. doi: 10.1021/ja405010v
  18. Kim, W.-J.; Kim, A.; Huh, C.; Park, C.W.; Ah, C.S.; Kim, B.K.; Yang, J.-H.; Chung, K.H.; Choi, Y.H.; Hong, J. et al. Photo selective protein immobilization using bovine serum albumin. Applied Surface Science, 2012, 261, 880-889. doi: 10.1016/j.apsusc.2012.08.111
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