sulfo-Cyanine3 NHS ester
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11320 | 1 mg | $91 | in stock | |
| 21320 | 5 mg | $119 | in stock | |
| 41320 | 25 mg | $395 | in stock | |
| 51320 | 50 mg |
$729
|
in stock | |
| 61320 | 100 mg |
$1290
|
in stock |
Water soluble, amino-reactive sulfo-Cyanine3 NHS ester that efficiently labels proteins and peptides in purely aqueous solution without needing organic co-solvent. Ideal for proteins with low solubility and proteins prone to denaturation.
This is the sulfonated, hydrophilic, and water-soluble dye. Non-sulfonated Cyanine3 NHS ester is also available.
This product is an analog of Cy3® NHS ester. sulfo-Cyanine3 NHS ester replaces Cy3® and DyLight 549 for all applications.
sulfo-Cyanine3 absorbance and emission spectra
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A tyramide derivative of AF 594, a red fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, and FISH.General properties
| Appearance: | dark red crystals |
| Molecular weight: | 751.91 |
| CAS number: | 1424150-38-8 (sodium salt); 1424433-17-9, 1518643-34-9 (inner salt) |
| Molecular formula: | C34H38N3KO10S2 |
| IUPAC name: | 3H-Indolium, 2-[3-(1,3-dihydro-1,3,3-trimethyl-5-sulfo-2H-indol-2-ylidene)-1-propen-1-yl]-1-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-3,3-dimethyl-5-sulfo-, inner salt, sodium salt |
| Solubility: | soluble in water (0.62 M = 47 g/L), in polar organic solvents (DMF, DMSO) |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 548 |
| ε, L⋅mol−1⋅cm−1: | 162000 |
| Emission maximum, nm: | 563 |
| Fluorescence quantum yield: | 0.1 |
| CF260: | 0.03 |
| CF280: | 0.06 |
Product citations
- Yang, B.; Picchetti, P.; Wang, Y.; Wang, W.; Seeger, C.; Bozov, K.; Malik, S.; Mallach, D.; Schäfer, A.H.; Ibrahim, M.; Hirtz, M.; Powell, A.K. Patterned immobilization of polyoxometalate-loaded mesoporous silica particles via amine-ene Michael additions on alkene functionalized surfaces. Scientific Reports, 2024, 14, 1249. doi: 10.1038/s41598-023-50846-2
- Zhao, J.; Elgeti, M.; O’Brien, E.S.; Sár, C.P.; EI Daibani, A.; Heng, J.; Sun, X.; White, E.; Che, T.; Hubbell, W.L.; Kobilka, B.K.; Chen, C. Ligand efficacy modulates conformational dynamics of the µ-opioid receptor. Nature, 2024, 629(8011), 474-480. doi: 10.1038/s41586-024-07295-2
- Lebedeva, N.A.; Anarbaev, R.O.; Maltseva, E.A.; Sukhanova, M.V.; Rechkunova, N.I.; Lavrik, O.I. DNA Repair Protein XRCC1 Stimulates Activity of DNA Polymerase λ under Conditions of Microphase Separation. International Journal of Molecular Sciences, 2024, 25(13), 6927. doi: 10.3390/ijms25136927
- Peña-Díaz, S.; Chao, J.D.; Rens, C.; Haghdadi, H.; Zheng, X.; Flanagan, K.; Ko, M.; Shapira, T.; Richter, A.; Maestre-Batlle, D.; Canseco, J.O.; Gutierrez, M.G.; Duc, K.D.; Pelech, S.; Av-Gay, Y. Glycogen synthase kinase 3 inhibition controls Mycobacterium tuberculosis infection. iScience, 2024, 27(8), 110555. doi: 10.1016/j.isci.2024.110555
Cy® is a trademark of Cytiva.
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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