Sulfo-Cyanine3 NHS ester

Cat. # Quantity Price Lead time
11320 1 mg $110.00 in stock
21320 5 mg $210.00 in stock
41320 25 mg $410.00 in stock
51320 50 mg $695.00 in stock
61320 100 mg $1190.00 in stock

Water soluble, amino-reactive sulfo-Cyanine3 NHS ester. Efficiently labels proteins and peptides in purely aqueous solution, without need for organic co-solvent. Ideal for proteins with low solubility, and proteins prone to denaturation.

This is sulfonated, hydrophilic and water-soluble dye. Non-sulfonated Cyanine3 NHS ester is also available.

This product is an analog of Cy3® NHS ester. Sulfo-Cyanine3 NHS ester replaces Cy3® and DyLight 549 for all applications.

Sulfo-Cyanine3 absorbance and emission spectra

Sulfo-Cyanine3 absorbance and emission spectra

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General properties

Appearance: dark red crystals
Molecular weight: 751.91
CAS number: 1424150-38-8 (sodium salt); 1424433-17-9, 1518643-34-9 (inner salt)
Molecular formula: C34H38N3KO10S2
IUPAC name: 3H-​Indolium, 2-​[3-​(1,​3-​dihydro-​1,​3,​3-​trimethyl-​5-​sulfo-​2H-​indol-​2-​ylidene)​-​1-​propen-​1-​yl]​-​1-​[6-​[(2,​5-​dioxo-​1-​pyrrolidinyl)​oxy]​-​6-​oxohexyl]​-​3,​3-​dimethyl-​5-​sulfo-​, inner salt, sodium salt
Solubility: soluble in water (0.62 M = 47 g/L), in polar organic solvents (DMF, DMSO)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 548
ε, L⋅mol−1⋅cm−1: 162000
Emission maximum, nm: 563
Fluorescence quantum yield: 0.1
CF260: 0.03
CF280: 0.06

Product citations

  1. Grohme, M.; Frank, O.; Rink, J. Preparing Planarian Cells for High-content Fluorescence Microscopy Using RNA in situ Hybridization and Immunocytochemistry. preprints.org, preprint. doi: 10.20944/preprints202111.0256.v1
  2. Futamata, H.; Fukuda, M.; Umeda, R.; Yamashita, K.; Takahashi, S.; Shikakura, Ta.; Hayashi, S.; Homma, K.; Nishizawa, T.; Nureki, O. Cryo-EM structures reveal the electromotility mechanism of prestin, the cochlear amplifier. Research Square, preprint. doi: 10.21203/rs.3.rs-927386/v1
  3. Lee, M.S.; Hyun, H.; Park, I.; Kim, S.; Jang, D.-H.; Kim, S.; Im, J.-K.; Kim, H.; Lee, J.H.; Kwon, T.; Kang, J.H. Quantitative fluorescence in situ hybridization (FISH) of magnetically confined bacteria enables rapid determination of early-stage human bacteremia. medRxiv, preprint. doi: 10.1101/2021.02.19.21251962
  4. Baker, Y.; Yuan, L.; Chen J.; Belle, R.; Carlisle, R.; El-Sagheer, A.; Brown, T. Expanding the Chemical Repertoire of DNA Nanomaterials Generated by Rolling Circle Amplification. ChemRxiv, preprint. doi: 10.26434/chemrxiv.12566963.v1
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