sulfo-Cyanine7 NHS ester

Cat. # Quantity Price Lead time
15320 1 mg $125.00 in stock
25320 5 mg $325.00 in stock
45320 25 mg $850.00 in stock
55320 50 mg $1490.00 in stock
65320 100 mg $1990.00 in stock

Water-soluble near-infrared dye sulfo-Cyanine7, an amine-reactive succinimide ester.

sulfo-Cyanine7 is an improved analog of Cy7® fluorophore with quantum yield improved by 20%, and higher photostability. This fluorescent dye is especially useful for NIR imaging.

Near-infrared fluorescent imaging takes advantage of the transparency of biological tissues at a particular range of wavelengths. The method is non-destructive and allows to monitor of the distribution of various labeled molecules in live organisms.

sulfo-Cyanine7 NHS ester reagent allows to prepare sulfo-Cyanine7-labeled biomolecules, such as proteins, with ease. Dye-labeled molecules can be subsequently used for various research and drug design-related experiments.

This reagent has high water solubility and is especially useful for labeling delicate proteins and proteins prone to denaturation. Non-sulfonated Cyanine7 NHS ester soluble in the organic phase is also available.

Sulfo-Cyanine7 absorbance and emission spectra

Sulfo-Cyanine7 absorbance and emission spectra

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Alkyne maleimide

Maleimide reagent for the attachment of alkyne functional group to proteins, peptides, and other molecules bearing SH (thiol) groups, including cysteine residues.

General properties

Appearance: dark green powder
Molecular weight: 844.05
CAS number: 1603861-95-5 (potassium salt); 1604244-45-2 (inner salt); 477908-53-5 (N-Ethyl)
Molecular formula: C41H46N3KO10S2
Solubility: good in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 750
ε, L⋅mol−1⋅cm−1: 240600
Emission maximum, nm: 773
Fluorescence quantum yield: 0.24
CF260: 0.04
CF280: 0.04

Product citations

  1. Barberio, A. E.; Smith, S. G.; Pires, I. S.; Iyer, S.; Reinhardt, F.; Melo, M. B.; Suh, H.; Weinberg, R. A.; Irvine, D. J.; Hammond, P. T. Layer-by-Layer Interleukin-12 Nanoparticles Drive a Safe and Effective Response in Ovarian Tumors. Bioengineering & Translational Medicine, 2023, 8(2), e10453. doi: 10.1002/btm2.10453
  2. Sandberg, E.; Piguet, J.; Kostiv, U.; Baryshnikov, G.; Liu, H.; Widengren, J. Photoisomerization of Heptamethine Cyanine Dyes Results in Red-Emissive Species: Implications for Near-IR, Single-Molecule, and Super-Resolution Fluorescence Spectroscopy and Imaging. J Phys Chem B, 2023, 127(14), 3208–3222. doi: 10.1021/acs.jpcb.2c08016
  3. White, M. J.; Raczy, M. M.; Budina, E.; Yuba, E.; Solanki, A.; Shim, H.-N.; Zhang, Z. J.; Gray, L. T.; Cao, S.; Alpar, A. T.; Hubbell, J. A. Engineered Collagen-Targeting Therapeutics Reverse Lung and Kidney Fibrosis in Mice. bioRxiv, 2022, preprint. doi: 10.1101/2022.01.04.474747
  4. Kang, Y.; Nack, L.M.; Liu, Yang and.; Qi, B.; Huang, Y.; Liu, Z.; Chakraborty, I.; Schulz, F.; Ahmed, A.A.A.; Poveda, M.C.; Hafizi, F.; Roy, S.; Mutas, M.; Holzapfel, M.; Sanchez-Cano, C.; Wegner, K.D.; Feliu, N.; Parak, W.J. Quantitative considerations about the size dependence of cellular entry and excretion of colloidal nanoparticles for different cell types. ChemTexts, 2022, 8(1), 9. doi: 10.1007/s40828-021-00159-6
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