Cyanine3 azide

Cat. # Quantity Price Lead time
11030 100 uL, 10 mM/DMSO $110.00 7 days
31030 500 uL, 10 mM/DMSO $210.00 7 days
41030 1 mL, 10 mM/DMSO $410.00 in stock
A1030 1 mg $110.00 in stock
B1030 5 mg $210.00 in stock
C1030 10 mg $310.00 in stock
D1030 25 mg $410.00 in stock
E1030 50 mg $695.00 in stock
F1030 100 mg $1190.00 in stock

Cyanine3 dye azide for Click Chemistry, an analog of Cy3® azide. Cy3® is one of the most broadly used fluorophores which can be detected by various fluorometers, imagers, and microscopes. Due to inherently high extinction coefficient, Cyanine3 is also easily detected by naked eye on gels, and in solution. This is non-sulfonated dye which requires organic co-solvent (DMF, DMSO, or other) for efficient labeling in water. Water-soluble version of this reagent is also available.

Product is available both as solid compound, and as 10 mM solution in DMSO which is ready to use in our recommended protocol.

Cyanine3 fluorescent properties are identical to Cy3®, and similar to Alexa Fluor 546, and DyLight 549.

Cy3 absorbance and emission spectra

Cy3 absorbance and emission spectra

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General properties

Appearance: red powder / solution
Molecular weight: 575.19
CAS number: 1167421-28-4 (chloride), 1167617-29-9 (without anion)
Molecular formula: C33H43N6OCl
Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), practically insoluble in water (40 mg/L = 60 uM)
Quality control: NMR 1H and HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 555
ε, L⋅mol−1⋅cm−1: 150000
Emission maximum, nm: 570
Fluorescence quantum yield: 0.31
CF260: 0.04
CF280: 0.09

Product citations

  1. Saqr, A.; Vakili, M.R.; Huang, Y.-H.; Lai, R.; Lavasanifar, A. Development of Traceable Rituximab-Modified PEO-Polyester Micelles by Postinsertion of PEG-phospholipids for Targeting of B-cell Lymphoma. ACS Omega, 2019, 4(20), 18867–18879. doi: 10.1021/acsomega.9b02910
  2. Morimoto, K.; Cole, K.S.; Kourelis, J.; Witt, C.H.; Brown, D.; Krahn, D.; Stegmann, M.; Kaschani, F.; Kaiser, M.; Burton, J.; Mohammed, S.; Yamaguchi-Shinozaki, K.; Weerapana, E.; van der Hoorn, R.A.L. Triazine Probes Target Ascorbate Peroxidases in Plants. Plant Physiology, 2019, 180(4), 1848–1859. doi: 10.1104/pp.19.00481
  3. Antonov, S.A.; Novosadova, E.V.; Kobylansky, A.G.; Tarantul, V.Z.; Grivennikov, I.A. A Hybrid Detection Method Based on Peroxidase-mediated Signal Amplification and Click Chemistry for Highly Sensitive Background-free Immunofluorescent Staining. Journal of Histochemistry & Cytochemistry, 2019, 67(10), 771–782. doi: 10.1369/0022155419864113
  4. Kasprzyk, R.; Starek, B.J.; Ciechanowicz, S.; Kubacka, D.; Kowalska, J.; Jemielity, J. Fluorescent turn-on probes for the development of binding and hydrolytic activity assays for mRNA cap-recognising proteins. Chemistry, 2019, 25(27), 6728–6740. doi: 10.1002/chem.201900051
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