Cyanine5 hydrazide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 13070 | 1 mg | $110.00 | in stock | |
| 23070 | 5 mg | $210.00 | in stock | |
| 43070 | 25 mg |
$410.00
|
5 days | |
| 53070 | 50 mg |
$695.00
|
in stock | |
| 63070 | 100 mg |
$1190.00
|
in stock |
Cyanine5 hydrazide is a reactive dye for the labeling of aldehydes and ketones, an analog of Cy5® hydrazide.
This dye reacts smoothly and nearly quantitatively with various carbonyl groups encountered in biomolecules. Examples are proteins subjected to oxidative stress, glycosylated proteins pre-activated by periodate oxidation (including antobodies), and oligonucleotides with aldehyde moieties.
Cyanine5 hydrazide replaces carbonyl-reactive Cy5®, Alexa Fluor 647, DyLight 649 dyes.
Cy5 absorbance and emission spectra
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General properties
| Appearance: | dark blue powder |
| Molecular weight: | 569.61 |
| Molecular formula: | C32H42Cl2N4O |
| Solubility: | moderate solubility in water, good in polar organic solvents (DMF, DMSO, alcohols) |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 250000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.2 |
| CF260: | 0.03 |
| CF280: | 0.04 |
Product citations
- Boone, C.; Grove, R.; Adamcova, D.; Braga, C.; Adamec, J. Revealing oxidative damage to enzymes of carbohydrate metabolism in yeast: An integration of 2D DIGE, quantitative proteomics and bioinformatics. Proteomics, 2016, 16(13), 1889–1903. doi: 10.1002/pmic.201500546
- Yoshimura, T.; Harashima, M.; Kurogi, K.; Suiko, M.; Liu, M.-C.; Sakakibara, Y. A novel procedure for the assessment of the antioxidant capacity of food components. Analytical Biochemistry, 2016, 507, 7–12. doi: 10.1016/j.ab.2016.05.002
- Huang, J.; Smith, F.; Panizzi, J.R.; Goodwin, D.C.; Panizzi, P. Inactivation of myeloperoxidase by benzoic acid hydrazide. Archives of Biochemistry and Biophysics, 2015, 570, 14–22. doi: 10.1016/j.abb.2015.01.028
- Einfalt, T.; Goers, R.; Dinu, I.A.; Najer, A.; Spulber, M.; Onaca-Fischer, O.; Palivan, C.G. Stimuli-triggered activity of nanoreactors by biomimetic engineering polymer membranes. Nano Letters, 2015, 15(11), 7596–7603. doi: 10.1021/acs.nanolett.5b03386
Cy® is a trademark of GE Healthcare.
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