Cyanine5 hydrazide

Cat. # Quantity Price Lead time
13070 1 mg $95 in stock
23070 5 mg $199 in stock
43070 25 mg $495 in stock
53070 50 mg $895 in stock
63070 100 mg $1490 in stock
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Cyanine5 hydrazide is a reactive dye for the labeling of aldehydes and ketones, an analog of Cy5® hydrazide.

This dye reacts smoothly and nearly quantitatively with various carbonyl groups encountered in biomolecules. Examples are proteins subjected to oxidative stress, glycosylated proteins pre-activated by periodate oxidation (including antobodies), and oligonucleotides with aldehyde moieties.

Cyanine5 hydrazide replaces carbonyl-reactive Cy5®, DyLight 649 dyes.

Cyanine5 absorbance and emission spectra

Cyanine5 absorbance and emission spectra

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General properties

Appearance: dark blue powder
Molecular weight: 569.61
CAS number: 1427705-31-4
Molecular formula: C32H42Cl2N4O
Solubility: moderate solubility in water, good in polar organic solvents (DMF, DMSO, alcohols)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2
CF260: 0.03
CF280: 0.04

Product citations

  1. Valero, J.; Civit, L.; Dupont, D. M.; Selnihhin, D.; Reinert, L. S.; Idorn, M.; Israels, B. A.; Bednarz, A. M.; Bus, C.; Asbach, B.; Peterhoff, D.; Pedersen, F. S.; Birkedal, V.; Wagner, R.; Paludan, S. R.; Kjems, J. A Serum-Stable RNA Aptamer Specific for SARS-CoV-2 Neutralizes Viral Entry. Proc. Natl. Acad. Sci. U.S.A., 2021, 118(50), e2112942118. doi: 10.1073/pnas.2112942118
  2. Sargiacomo, C.; Stonehouse, S.; Moftakhar, Z.; Sotgia, F.; Lisanti, M.P. MitoTracker Deep Red (MTDR) Is a Metabolic Inhibitor for Targeting Mitochondria and Eradicating Cancer Stem Cells (CSCs), With Anti-Tumor and Anti-Metastatic Activity In Vivo. Frontiers in Oncology, 2021, 11, 678343. doi: 10.3389/fonc.2021.678343
  3. Sekulovski, S.; Devant, P.; Panizza, S.; Gogakos, T.; Pitiriciu, A.; Heitmeier, K.; Ramsay, E.P.; Barth, M.; Schmidt, C.; Tuschl, T.; Baas, F.; Weitzer, S.; Martinez, J.; Trowitzsch, S. Assembly defects of human tRNA splicing endonuclease contribute to impaired pre-tRNA processing in pontocerebellar hypoplasia. Nature Communications, 2021, 12, 5610. doi: 10.1038/s41467-021-25870-3
  4. Lix, K.; Krause, K.D.; Kim, H.; Algar, W.R. Investigation of the Energy Transfer Mechanism Between Semiconducting Polymer Dots and Organic Dyes. Journal of Physical Chemistry C, 2020, 124(31), 17387–17400. doi: 10.1021/acs.jpcc.0c04983
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