Cyanine5 hydrazide

Cat. # Quantity Price Lead time
13070 1 mg $110.00 in stock
23070 5 mg $210.00 in stock
43070 25 mg $410.00 in stock
53070 50 mg $695.00 in stock
63070 100 mg $1190.00 in stock

Cyanine5 hydrazide is a reactive dye for the labeling of aldehydes and ketones, an analog of Cy5® hydrazide.

This dye reacts smoothly and nearly quantitatively with various carbonyl groups encountered in biomolecules. Examples are proteins subjected to oxidative stress, glycosylated proteins pre-activated by periodate oxidation (including antobodies), and oligonucleotides with aldehyde moieties.

Cyanine5 hydrazide replaces carbonyl-reactive Cy5®, Alexa Fluor 647, DyLight 649 dyes.

Cy5 absorbance and emission spectra

Cy5 absorbance and emission spectra

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General properties

Appearance: dark blue powder
Molecular weight: 569.61
CAS number: 1427705-31-4
Molecular formula: C32H42Cl2N4O
Solubility: moderate solubility in water, good in polar organic solvents (DMF, DMSO, alcohols)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2
CF260: 0.03
CF280: 0.04

Product citations

  1. Lix, K.; Krause, K.D.; Kim, H.; Algar, W.R. Investigation of the Energy Transfer Mechanism Between Semiconducting Polymer Dots and Organic Dyes. Journal of Physical Chemistry C, 2020, 124(31), 17387–17400. doi: 10.1021/acs.jpcc.0c04983
  2. Imlimthan, S.; Correia, A.; Figueiredo, P.; Lintinen, K.; Balasubramanian, V.; Airaksinen, A.J.; Kostiainen, M.A.; Santos, H.A.; Sarparanta, M. Systematic In Vitro Biocompatibility Studies of Multimodal Cellulose Nanocrystal and Lignin Nanoparticles. Journal of Biomedical Materials Research. Part A, 2020, 108(3), 770–783. doi: 10.1002/jbm.a.36856
  3. Wang, J.; Johnson, A.G.; Lapointe, C.P.; Choi, J.; Prabhakar, A.; Chen, D.-H.; Petrov, A.N.; Puglisi, J.D. eIF5B gates the transition from translation initiation to elongation. Nature, 2019, 573, 605–608. doi: 10.1038/s41586-019-1561-0
  4. Boone, C.; Grove, R.; Adamcova, D.; Braga, C.; Adamec, J. Revealing oxidative damage to enzymes of carbohydrate metabolism in yeast: An integration of 2D DIGE, quantitative proteomics and bioinformatics. Proteomics, 2016, 16(13), 1889–1903. doi: 10.1002/pmic.201500546
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