FAM azide, 5-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 14130 | 100 uL, 10 mM/DMSO | $80.00 | in stock | |
| 34130 | 500 uL, 10 mM/DMSO | $100.00 | in stock | |
| 44130 | 1 mL, 10 mM/DMSO | $150.00 | in stock | |
| A4130 | 1 mg | $80.00 | in stock | |
| B4130 | 5 mg | $100.00 | in stock | |
| C4130 | 10 mg | $150.00 | in stock | |
| D4130 | 25 mg | $295.00 | in stock | |
| E4130 | 50 mg |
$450.00
|
in stock | |
| F4130 | 100 mg |
$780.00
|
in stock |
FAM azide for Click chemistry labeling. FAM remains one of the most popular fluorescent lablels for various application. Most instruments capable of fluorescence detection, ranging from plate readers to fluorescence microscopes, are able to work in FAM channel.
With versatility of Click chemistry and this reagent, it is possible to attach this popular fluorophore to nearly any alkyne bearing molecule.
FAM azide is available both as solid compound, and as 10 mM solution in DMSO ready to use in our recommended labeling protocol. This product is a pure 5-isomer. FAM is a replacement for Alexa Fluor 488, DyLight 488.
Recommended protocol
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BDP TMR azide
Borondipyrromethene dye with azido group. Spectral properties are similar to TAMRA dye. Due to long fluorescence lifetime, BDP TMR is especially useful for fluorescence polarization measurements.EdU (5-ethynyl-2'-deoxyuridine)
EdU is a nucleoside which is incorporated into replicated DNA by cellular enzymes. After it, DNA contained in the cells can be developed by Click chemistry reaction with fluorescent dye azides to reveal cell proliferation.BDP TR azide
BDP TR is a red emitting fluorophore for Texas Red (ROX) channel. This is an azide for copper catalyzed and copper free Click chemistry conjugation.General properties
| Appearance: | yellowish crystals |
| Molecular weight: | 458.42 |
| CAS number: | 510758-23-3 |
| Molecular formula: | C24H18N4O6 |
| Solubility: | soluble in polar organic solvents (DMF, DMSO, alcohols) |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 494 |
| ε, L⋅mol−1⋅cm−1: | 75000 |
| Emission maximum, nm: | 520 |
| Fluorescence quantum yield: | 0.9 |
| CF260: | 0.20 |
| CF280: | 0.17 |
Product citations
- Haider, N.; Dutt, P.; van de Kooij, B.; Yaffe, M.B.; Stambolic, V. NEK10 tyrosine phosphorylates p53 and controls its transcriptional activity. bioRxiv, preprint. doi: 10.1101/516971
- Nozeret, K.; Boucharlat, A.; Agou, F.; Buddelmeijer, N. A sensitive fluorescence-based assay to monitor enzymatic activity of the essential integral membrane protein Apolipoprotein N-acyltransferase (Lnt). Scientific Reports, 2019, 9, 15978. doi: 10.1038/s41598-019-52106-8
- Synakewicz, M.; Bauer, D.; Rief, M.; Itzhaki, L.S. Bioorthogonal protein-DNA conjugation methods for force spectroscopy. Scientific Reports, 2019, 9, 13820. doi: 10.1038/s41598-019-49843-1
- Son, K.; You, J.-S.; Yoon, M.-S.; Dai, C.; Kim, J.H.; Khanna, N.; Banerjee, A.; Martinis, S.A.; Han, G.; Han, J.M.; Kim, S.; Chen, J. Nontranslational function of leucyl-tRNA synthetase regulates myogenic differentiation and skeletal muscle regeneration. Journal of Clinical Investigation, 2019, 130, 2088–2093. doi: 10.1172/JCI122560
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