FAM azide, 5-isomer

Cat. # Quantity Price Lead time
14130 100 uL, 10 mM/DMSO $69 in stock
34130 500 uL, 10 mM/DMSO $97 in stock
44130 1 mL, 10 mM/DMSO $139 in stock
A4130 1 mg $69 in stock
B4130 5 mg $97 in stock
C4130 10 mg $139 in stock
D4130 25 mg $285 in stock
E4130 50 mg $445 in stock
F4130 100 mg $765 in stock
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FAM azide for сlick chemistry labeling. FAM remains one of the most popular fluorescent labels for various applications. Most instruments capable of fluorescence detection, ranging from plate readers to fluorescence microscopes, are able to work in the FAM channel.

With the versatility of сlick chemistry and this reagent, it is possible to attach this popular fluorophore to nearly any alkyne-bearing molecule.

FAM azide is available both as a solid compound and as a 10 mM solution in DMSO ready to use in our recommended labeling protocol. This product is a pure 5-isomer. FAM is a replacement for DyLight 488.

Absorption and emission spectra of FAM

Absorption and emission spectra of FAM

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General properties

Appearance: yellowish crystals
Molecular weight: 458.42
CAS number: 510758-23-3
Molecular formula: C24H18N4O6
Solubility: soluble in polar organic solvents (DMF, DMSO, alcohols)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 492
ε, L⋅mol−1⋅cm−1: 74000
Emission maximum, nm: 517
Fluorescence quantum yield: 0.93
CF260: 0.22
CF280: 0.17

Product citations

  1. Ho H’ng, C.; Amarasinghe, S.L.; Zhang, B.; Chang, H.; Qu, X.; Powell, D.R.; Rosello-Diez, A. Compensatory growth and recovery of cartilage cytoarchitecture after transient cell death in fetal mouse limbs. Nature Communications, 2024, 15, 2940. doi: 10.1038/s41467-024-47311-7
  2. Perzanowska, O.; Smietanski, M.; Jemielity, J.; Kowalska, J. Chemically Modified Poly(A) Analogs Targeting PABP: Structure Activity Relationship and Translation Inhibitory Properties. Chemistry – A European Journal, 2022, 28(42), e202201115. doi: 10.1002/chem.202201115
  3. Olson, S. W.; Turner, A.-M. W.; Arney, J. W.; Saleem, I.; Weidmann, C. A.; Margolis, D. M.; Weeks, K. M.; Mustoe, A. M. Discovery of a Large-Scale, Cell-State-Responsive Allosteric Switch in the 7SK RNA Using DANCE-MaP. Molecular Cell, 2022, 82(9), 1708-1723.e10. doi: 10.1016/j.molcel.2022.02.009
  4. Gueiderikh, A.; Maczkowiak-Chartois, F.; Rouvet, G.; Souquère-Besse, S.; Apcher, S.; Diaz, J.-J.; Rosselli, F. Fanconi anemia A protein participates in nucleolar homeostasis maintenance and ribosome biogenesis. Science Advances, 2021, 7(1), eabb5414. doi: 10.1126/sciadv.abb5414
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