FAM azide, 5-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 14130 | 100 uL, 10 mM/DMSO | $80.00 | in stock | |
| 34130 | 500 uL, 10 mM/DMSO | $100.00 | in stock | |
| 44130 | 1 mL, 10 mM/DMSO | $150.00 | in stock | |
| A4130 | 1 mg | $80.00 | in stock | |
| B4130 | 5 mg | $100.00 | in stock | |
| C4130 | 10 mg | $150.00 | in stock | |
| D4130 | 25 mg | $295.00 | in stock | |
| E4130 | 50 mg |
$450.00
|
in stock | |
| F4130 | 100 mg |
$780.00
|
in stock |
FAM azide for Click chemistry labeling. FAM remains one of the most popular fluorescent lablels for various application. Most instruments capable of fluorescence detection, ranging from plate readers to fluorescence microscopes, are able to work in FAM channel.
With versatility of Click chemistry and this reagent, it is possible to attach this popular fluorophore to nearly any alkyne bearing molecule.
FAM azide is available both as solid compound, and as 10 mM solution in DMSO ready to use in our recommended labeling protocol. This product is a pure 5-isomer. FAM is a replacement for Alexa Fluor 488, DyLight 488.
Recommended protocol
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Cyanine5 azide
Cyanine5 azide is a reagent for Click Chemistry labeling with Cyanine5, a popular fluorescent dye.Copper(II)-TBTA complex, 10 mM in 55% aq. DMSO
Catalyst for bioconjugate synthesis by Click Chemistry.Sulfo-Cyanine5.5 NHS ester
Water soluble, sulfonated, far-red sulfo-Cyanine5.5 dye in the form of NHS ester.General properties
| Appearance: | yellowish crystals |
| Molecular weight: | 458.42 |
| CAS number: | 510758-23-3 |
| Molecular formula: | C24H18N4O6 |
| Solubility: | soluble in polar organic solvents (DMF, DMSO, alcohols) |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 494 |
| ε, L⋅mol−1⋅cm−1: | 75000 |
| Emission maximum, nm: | 520 |
| Fluorescence quantum yield: | 0.9 |
| CF260: | 0.20 |
| CF280: | 0.17 |
Product citations
- Haider, N.; Dutt, P.; van de Kooij, B.; Yaffe, M.B.; Stambolic, V. NEK10 tyrosine phosphorylates p53 and controls its transcriptional activity. bioRxiv, preprint. doi: 10.1101/516971
- Gueiderikh, A.; Maczkowiak-Chartois, F.; Rouvet, G.; Souquère-Besse, S.; Apcher, S.; Diaz, J.-J.; Rosselli, F. Fanconi anemia A protein participates in nucleolar homeostasis maintenance and ribosome biogenesis. Science Advances, 2021, 7(1), eabb5414. doi: 10.1126/sciadv.abb5414
- Ramey-Ward, A.N.; Su, H.; Salaita, K. Mechanical stimulation of adhesion receptors using light-responsive nanoparticle actuators enhances myogenesis. ACS Applied Materials & Interfaces, 2020, 12(32), 35903–35917. doi: 10.1021/acsami.0c08871
- Kim, D.; Singh, N.; Waldemer-Streyer, R.J.; Yoon, M.-S.; Chen, J. Muscle-derived TRAIL negatively regulates myogenic differentiation. Experimental Cell Research, 2020, 394(1), 112165. doi: 10.1016/j.yexcr.2020.112165
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