FAM azide, 5-isomer

Fluorescein (FAM) Azides Fluorescence polarization Microscopy
Cat. # Quantity Price Lead time
14130 100 uL, 10 mM/DMSO $80.00 in stock
34130 500 uL, 10 mM/DMSO $100.00 in stock
44130 1 mL, 10 mM/DMSO $150.00 in stock
A4130 1 mg $80.00 in stock
B4130 5 mg $100.00 in stock
C4130 10 mg $150.00 in stock
D4130 25 mg $295.00 in stock
E4130 50 mg $450.00 in stock
F4130 100 mg $780.00 in stock
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FAM azide for Click chemistry labeling. FAM remains one of the most popular fluorescent lablels for various application. Most instruments capable of fluorescence detection, ranging from plate readers to fluorescence microscopes, are able to work in FAM channel.

With versatility of Click chemistry and this reagent, it is possible to attach this popular fluorophore to nearly any alkyne bearing molecule.

FAM azide is available both as solid compound, and as 10 mM solution in DMSO ready to use in our recommended labeling protocol. This product is a pure 5-isomer. FAM is a replacement for Alexa Fluor 488, DyLight 488.

FAM azide, 5-isomer, azide stock solution for click chemistry (10 mM in DMSO), 14130
FAM azide, 5-isomer, azide stock solution for click chemistry (10 mM in DMSO), 14130
FAM azide, 5-isomer, azide stock solution for click chemistry (10 mM in DMSO), 14130
FAM azide, 5-isomer, E4130
FAM azide, 5-isomer, E4130
5-FAM azide structure
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Recommended protocol

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General properties

Appearance: yellowish crystals
Molecular weight: 458.42
Molecular formula: C24H18N4O6
Solubility: soluble in polar organic solvents (DMF, DMSO, alcohols)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 494
ε, L⋅mol−1⋅cm−1: 75000
Emission maximum, nm: 520
Fluorescence quantum yield: 0.9
CF260: 0.20
CF280: 0.17

Product citations

  1. Haider, N.; Dutt, P.; van de Kooij, B.; Yaffe, M.B.; Stambolic, V. NEK10 tyrosine phosphorylates p53 and controls its transcriptional activity. bioRxiv, preprint. doi: 10.1101/516971
  2. Feltes, M.; Moores, S.; Gale, S.E.; Krishnan, K.; Mydock-McGrane, L.; Covey, D.F.; Ory, D.S.; Schaffer, J.E. Synthesis and characterization of diazirine alkyne probes for the study of intracellular cholesterol trafficking. Journal of Lipid Research, 2019, 60(3), 707–716. doi: 10.1194/jlr.D091470
  3. Delasoie, J.; Rossier, J.; Haeni, L.; Rothen-Rutishauser, B.; Zobi, F. Slow-targeted release of a ruthenium anticancer agent from vitamin B12 functionalized marine diatom microalgae. Dalton Transactions, 2018, 47(48), 17221–17232. doi: 10.1039/c8dt02914h
  4. Ta, D.T.; Vanella, R.; Nash, M.A. Bioorthogonal Elastin-like Polypeptide Scaffolds for Immunoassay Enhancement. ACS Applied Materials & Interfaces, 2018, 10(36), 30147–30154. doi: 10.1021/acsami.8b10092
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