FAM azide, 5-isomer

Cat. # Quantity Price Lead time
14130 100 uL, 10 mM/DMSO $80.00 in stock
34130 500 uL, 10 mM/DMSO $100.00 5 days
44130 1 mL, 10 mM/DMSO $150.00 in stock
A4130 1 mg $80.00 in stock
B4130 5 mg $100.00 in stock
C4130 10 mg $150.00 in stock
D4130 25 mg $295.00 in stock
E4130 50 mg $450.00 in stock
F4130 100 mg $780.00 in stock

FAM azide for Click chemistry labeling. FAM remains one of the most popular fluorescent lablels for various application. Most instruments capable of fluorescence detection, ranging from plate readers to fluorescence microscopes, are able to work in FAM channel.

With versatility of Click chemistry and this reagent, it is possible to attach this popular fluorophore to nearly any alkyne bearing molecule.

FAM azide is available both as solid compound, and as 10 mM solution in DMSO ready to use in our recommended labeling protocol. This product is a pure 5-isomer. FAM is a replacement for Alexa Fluor 488, DyLight 488.

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Acetylenic amidite for the incorporation of acetylene groups into oligos.

General properties

Appearance: yellowish crystals
Molecular weight: 458.42
Molecular formula: C24H18N4O6
Solubility: soluble in polar organic solvents (DMF, DMSO, alcohols)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download

Spectral properties

Excitation maximum, nm: 494
ε, L⋅mol−1⋅cm−1: 75000
Emission maximum, nm: 520
Fluorescence quantum yield: 0.9
CF260: 0.20
CF280: 0.17

Product citations

  1. Pink, M.; Verma, N.; Zerries, A.; Schmitz-Spanke, S. Dose-dependent response to 3-nitrobenzanthrone exposure in human urothelial cancer cells. Chemical Research in Toxicology, 2017, 30(10), 1855–1864. doi: 10.1021/acs.chemrestox.7b00174
  2. Guttenplan, A.P.M.; Young, L.J.; Matak-Vinkovic, D.; Kaminski, C.F.; Knowles, T.P.J.; Itzhaki, L.S. Nanoscale click-reactive scaffolds from peptide self-assembly. Journal of Nanobiotechnology, 2017, 15, 70. doi: 10.1186/s12951-017-0300-7
  3. Ruhl, K.E; Rovis, T. Visible Light-Gated Cobalt Catalysis for a Spatially and Temporally Resolved [2+2+2] Cycloaddition. Journal of the American Chemical Society, 2016, 138(48), 15527–15530. doi: 10.1021/jacs.6b08792
  4. Berte, N.; Piee-Staffa, A.; Piecha, N.; Wang, M.; Borgmann, K.; Kaina, B.; Nikolova, T. Targeting homologous recombination by pharmacological inhibitors enhances the killing response of glioblastoma cells treated with alkylating drugs. Molecular Cancer Therapeutics, 2016, 15(11), 2665–2678. doi: 10.1158/1535-7163.mct-16-0176
  5. Ngo, J.T.; Adams, S.R.; Deerinck, T.J.; Boassa, D.; Rodriguez-Rivera, F.; Palida, S.F.; Bertozzi, C.R.; Ellisman, M.H.; Tsien, R.Y. Click-EM for imaging metabolically tagged nonprotein biomolecules. Nature Chemical Biology, 2016, 12(6), 459–465. doi: 10.1038/nchembio.2076
  6. Li, Z.; Liu, Z.; Chen, Z.; Ju, E.; Li, W.; Ren, J.; Qu, X. Bioorthogonal chemistry for selective recognition, separation and killing bacteria over mammalian cells. Chemical Communications, 2016, 52(17), 3482–3485. doi: 10.1039/c5cc10625g
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