NHS esters in Reactive groups

Primary or secondary amino groups are ubiquitous in biomolecules. They are present in proteins and peptides as ε-amino groups of lysines and N-terminal amino groups. Many natural and synthetic small molecules are amines, and DNAs with amino groups can be prepared by solid phase synthesis, and enzymatically.

Amino groups can be modified by acylation with various activated carbonyl compounds. Among these compounds, activated N-hydroxysuccinimide esters are most suitable for biolabeling and bioconjugation applications.

NHS ester coupling chemistry

Compared to other activated esters, NHS derivatives possess good stability in aqueous environment. Optimal pH for the coupling of amines with NHS esters in aqueous buffers is 8.3-8.5. Labeling is also possible in organic solvents. Polar aprotic solents, such as DMF or DMSO, are preferred. One equivalent of tertiary amine base, such as triethylamine, or diisopropylethylamine (DIPEA) is necessary to achieve efficient amine acylation in non-aqueous solution.

AF 488 antibody labeling kit

Ready-to-use kit for labeling antibodies and other proteins with AF 488 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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Cat. # Quantity Price Lead time
1821-10rxn
10 rxn
$450.00 1 days

AF 594 antibody labeling kit

Ready-to-use kit for labeling antibodies and other proteins with AF 594 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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Cat. # Quantity Price Lead time
6821-10rxn
10 rxn
$450.00 21 days

sulfo-Cyanine3 antibody labeling kit

Ready to use kit for the labeling of antibodies and other proteins with sulfo-Cyanine3 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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Cat. # Quantity Price Lead time
1321-10rxn
10 rxn
$450.00 1 days

sulfo-Cyanine5 antibody labeling kit

Ready-to-use kit for labeling antibodies and other proteins with sulfo-Cyanine5 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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Cat. # Quantity Price Lead time
3321-10rxn
10 rxn
$400.00 1 days

sulfo-Cyanine5.5 antibody labeling kit

Ready-to-use kit for labeling antibodies and other proteins with sulfo-Cyanine5.5 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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Cat. # Quantity Price Lead time
7321-10rxn
10 rxn
$450.00 1 days

sulfo-Cyanine7 antibody labeling kit

Ready-to-use kit for labeling antibodies and other proteins with sulfo-Cyanine7 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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Cat. # Quantity Price Lead time
5321-10rxn
10 rxn
$450.00 1 days

sulfo-Cyanine7.5 antibody labeling kit

Ready-to-use kit for labeling antibodies and other proteins with sulfo-Cyanine7.5 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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Cat. # Quantity Price Lead time
6321-10rxn
10 rxn
$450.00 1 days

(5,6)-FAM diacetate NHS ester

Amine-reactive fluorescein (FAM) derivative, a mixture of 5- and 6-isomers. The reagent becomes brightly green fluorescent once it is hydrolyzed while diffusing into cells. A fluorescent tracer.
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Cat. # Quantity Price Lead time
2934-25mg 25 mg $160.00 in stock
2934-50mg 50 mg $270.00 in stock
2934-100mg 100 mg $520.00 in stock

AF 343 (Coumarin) X NHS ester

AF 343 (Coumarin) is a blue emitting dye that can serve as a FRET donor for fluorescein (FAM). This structure features a linker between Coumarin 343 fluorophore, and the functional group. It provides better solubility, and spatial separation between the dye, and the functional group.
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Cat. # Quantity Price Lead time
12620 1 mg $110.00 in stock
22620 5 mg $210.00 in stock
42620 25 mg $410.00 in stock
52620 50 mg $695.00 in stock
62620 100 mg $1190.00 in stock

AF 430 NHS ester

AF 430 is a hydrophilic coumarin dye with emission in the green-yellow range. The NHS ester is used for the labeling of amine groups.
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Cat. # Quantity Price Lead time
10820 1 mg $110.00 in stock
20820 5 mg $165.00 in stock
40820 25 mg $380.00 in stock
50820 50 mg $695.00 in stock
60820 100 mg $1190.00 in stock

AF 488 NHS ester

AF 488 NHS ester, a bright, photostable, and hydrophilic fluorescent dye for the green channel.
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Cat. # Quantity Price Lead time
11820 1 mg $125.00 in stock
21820 5 mg $325.00 in stock
31820 10 mg $480.00 in stock
41820 25 mg $950.00 in stock
51820 50 mg $1625.00 in stock
61820 100 mg $2090.00 in stock

AF 532 NHS ester

NHS-ester of AF 532 dye for protein, peptide, and modified oligonucleotide labeling via reaction with amino groups.
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Cat. # Quantity Price Lead time
1C820 1 mg $140.00 in stock
2C820 5 mg $450.00 in stock
4C820 25 mg $1460.00 in stock
5C820 50 mg $1890.00 in stock
6C820 100 mg $2640.00 in stock

AF 555 NHS ester

AF 555 NHS ester, a bright, photostable, and hydrophilic fluorescent dye for the yellow channel.
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Cat. # Quantity Price Lead time
1K820 1 mg $140.00 in stock
2K820 5 mg $450.00 in stock
4K820 25 mg $1460.00 in stock
5K820 50 mg $1890.00 in stock
6K820 100 mg $2640.00 in stock

AF 568 NHS ester

AF 568 derivative for labeling proteins and other high- and low-molecular weight ligands with free amines.
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Cat. # Quantity Price Lead time
14820 1 mg $125.00 in stock
24820 5 mg $325.00 in stock
44820 25 mg $720.00 in stock
54820 50 mg $1325.00 in stock
64820 100 mg $2090.00 in stock

AF 594 NHS ester

Red-fluorescent NHS-ester of AF 594 dye for labeling proteins, peptides, antibodies, and any molecules containing an -NH2 group. Pure 5-isomer
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Cat. # Quantity Price Lead time
2825-1mg 1 mg $125.00 5 days
2825-5mg 5 mg $425.00 in stock
2825-25mg 25 mg $1240.00 in stock
2825-50mg 50 mg $1890.00 in stock
2825-100mg 100 mg $2640.00 in stock

AF 647 NHS ester

Bright, water-soluble succinimide ester of AF 647 dye for protein and antibody labeling.
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Cat. # Quantity Price Lead time
16820 1 mg $140.00 in stock
26820 5 mg $450.00 in stock
46820 25 mg $1460.00 in stock
56820 50 mg $1890.00 in stock
66820 100 mg $2640.00 in stock

Alkyne-PEG2-NHS ester

Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on diethyleneglycol (PEG2).
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Cat. # Quantity Price Lead time
2219-1g 1 g $450.00 in stock
2219-5g 5 g $980.00 in stock

Alkyne-PEG3-NHS ester

Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on triethyleneglycol (PEG3).
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Cat. # Quantity Price Lead time
2298-1g 1 g $490.00 in stock
2298-5g 5 g $980.00 in stock

Alkyne-PEG4-NHS ester

Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on tetraethyleneglycol (PEG4).
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Cat. # Quantity Price Lead time
2409-100mg 100 mg $250.00 5 days
2409-1g 1 g $790.00 in stock

AMCA NHS ester

NHS-ester of AMCA dye for protein, peptide, and modified oligonucleotide labeling via reaction with amino groups. AMCA is one of the brightest blue fluorescent dyes with large Stoke's shift and high resistance to photobleaching.
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Cat. # Quantity Price Lead time
3606-5mg 5 mg $110.00 in stock
3606-25mg 25 mg $290.00 in stock
3606-50mg 50 mg $435.00 in stock
3606-100mg 100 mg $595.00 in stock

Azide-PEG6-NHS ester

A hydrophilic bifunctional linker with azide and NHS ester function groups flanking PEG6 (hexaethylene glycol).
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Cat. # Quantity Price Lead time
3692-250mg 250 mg $325.00 in stock
3692-1g 1 g $539.00 in stock

Azidobutyric acid NHS ester

Activated NHS ester for azido-labeling of peptides and proteins.
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Cat. # Quantity Price Lead time
53720 50 mg $110.00 in stock
63720 100 mg $210.00 in stock
73720 1 g $950.00 in stock
83720 5 g please inquire in stock

BDP 558/568 NHS ester

BDP 558/568 is a fluorophore for the yellow region of the spectrum. Like other BDP dyes, this fluorophore has high fluorescence quantum yield, brightness, and photostability.
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Cat. # Quantity Price Lead time
17420 1 mg $125.00 in stock
27420 5 mg $260.00 in stock
47420 25 mg $510.00 in stock
57420 50 mg $895.00 in stock
67420 100 mg $1490.00 in stock

BDP 564/570 NHS ester

Lypohilic borodipyrromethene dye with orange emission for the labeling of primary and secondary amine groups.
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Cat. # Quantity Price Lead time
1H420 1 mg $125.00 in stock
2H420 5 mg $325.00 in stock
4H420 25 mg $510.00 in stock
5H420 50 mg $895.00 in stock
6H420 100 mg $1490.00 in stock

BDP 576/589 NHS ester

BDP 576/589 NHS ester is a bright hydrophobic dye with emission in the orange spectrum range. It can be used in fluorescence anisotropy assays.
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Cat. # Quantity Price Lead time
1G420 1 mg $125.00 in stock
2G420 5 mg $260.00 in stock
4G420 25 mg $510.00 in stock
5G420 50 mg $895.00 in stock
6G420 100 mg $1490.00 5 days

BDP 581/591 NHS ester

NHS ester derivative of BDP 581/591 borondipyrromethene fluorophore for the labeling of proteins, peptides, and other biomolecules. The dye is useful for fluorescence polarization and two-photon experiments, and as a ROS probe.
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Cat. # Quantity Price Lead time
16420 1 mg $125.00 in stock
26420 5 mg $260.00 in stock
46420 25 mg $510.00 in stock
56420 50 mg $895.00 in stock
66420 100 mg $1490.00 in stock

BDP 630/650 X NHS ester

BDP 630/650 is a borondipyrromethene fluorophore tuned to Cyanine5 excitation and emission channels. The dye is bright and highly photostable. It is useful for fluorescence polarization assays. This is an amine-reactive NHS ester derivative.
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Cat. # Quantity Price Lead time
15420 1 mg $125.00 in stock
25420 5 mg $260.00 in stock
45420 25 mg $510.00 in stock
55420 50 mg $895.00 in stock
65420 100 mg $1490.00 in stock

BDP 650/665 X NHS ester

Amine reactive of BDP 650/665, a borondipyrromethene dye for Cy5 channel, possessing a high molar extinction coefficient, and a good emission quantum yield.
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Cat. # Quantity Price Lead time
1E420 1 mg $125.00 in stock
2E420 5 mg $260.00 in stock
4E420 25 mg $510.00 in stock
5E420 50 mg $895.00 in stock
6E420 100 mg $1490.00 in stock

BDP FL NHS ester

Amino-reactive BDP FL, a very bright and photostable dye for fluorescein channel.
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Cat. # Quantity Price Lead time
11420 1 mg $125.00 in stock
21420 5 mg $260.00 in stock
41420 25 mg $510.00 in stock
51420 50 mg $895.00 in stock
61420 100 mg $1490.00 in stock

BDP R6G NHS ester

BDP R6G is a borondipyrromethene dye which has absorption and emission spectra similar to R6G.
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Cat. # Quantity Price Lead time
14420 1 mg $125.00 in stock
24420 5 mg $260.00 in stock
44420 25 mg $510.00 in stock
54420 50 mg $895.00 in stock
64420 100 mg $1490.00 in stock

BDP TMR NHS ester

Amine reactive NHS ester of BDP TMR, bright borondipyrromethene dye for TAMRA channel especially suitable for fluorescence polarization assays.
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Cat. # Quantity Price Lead time
12420 1 mg $125.00 in stock
22420 5 mg $260.00 in stock
42420 25 mg $510.00 in stock
52420 50 mg $895.00 in stock
62420 100 mg $1490.00 in stock

BDP TMR X NHS ester

Amine reactive NHS ester of BDP TMR, bright borondipyrromethene dye for TAMRA channel especially suitable for fluorescence polarization assays. This compound contains an aminohexanoic acid (C6) linker between the dye and the functional group.
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Cat. # Quantity Price Lead time
1F420 1 mg $125.00 in stock
2F420 5 mg $260.00 in stock
4F420 25 mg $510.00 in stock
5F420 50 mg $895.00 in stock
6F420 100 mg $1490.00 in stock

BDP TR NHS ester

BDP TR is a borondipyrromethene dye. A bright and photostable fluorophore with high quantum yield.
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Cat. # Quantity Price Lead time
NO items of this product available in stock but you can submit request for bulk.

BDP TR X NHS ester

BDP TR X NHS ester is a derivative of BDP TR containing a long linker based on aminohexanoic acid (C6). The dye has absorption and emission close to ROX. The NHS ester function can be used to conjugate proteins and peptides.
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Cat. # Quantity Price Lead time
1D420 1 mg $125.00 in stock
2D420 5 mg $325.00 in stock
4D420 25 mg $510.00 in stock
5D420 50 mg $895.00 in stock
6D420 100 mg $1490.00 in stock

Biotin X NHS ester

Biotin derivative with C6-spacer to reduce steric effect when binding amino acids, peptides, or proteins by reacting with primary amines.
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Cat. # Quantity Price Lead time
2551-100mg 100 mg $117.00 in stock
2551-1g 1 g please inquire in stock

Biotin-PEG4-NHS ester

PEG4-containing activated ester for simple and efficient biotin labeling of antibodies, proteins, and other primary amine-containing biomolecules.
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Cat. # Quantity Price Lead time
2893-100mg 100 mg $145.00 in stock
2893-1g 1 g $590.00 in stock

Cyanine2 NHS ester minimal dye

Cyanine2 NHS ester minimal dye for protein labeling
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Cat. # Quantity Price Lead time
1A041 5 nmol $110.00 in stock
2A041 10 nmol $140.00 in stock
3A041 25 nmol $265.00 in stock

Cyanine3 NHS ester

Amine-reactive Cyanine3 dye NHS ester.
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Cat. # Quantity Price Lead time
11020 1 mg $125.00 in stock
21020 5 mg $260.00 in stock
41020 25 mg $510.00 in stock
51020 50 mg $895.00 in stock
61020 100 mg $1490.00 in stock

Cyanine3 NHS ester minimal dye

Cyanine3 minimal dye for protein labeling.
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Cat. # Quantity Price Lead time
1B041 5 nmol $110.00 in stock
2B041 10 nmol $175.00 in stock
3B041 25 nmol $265.00 in stock