NHS esters in Reactive groups
Primary or secondary amino groups are ubiquitous in biomolecules. They are present in proteins and peptides as ε-amino groups of lysines and N-terminal amino groups. Many natural and synthetic small molecules are amines, and DNAs with amino groups can be prepared by solid phase synthesis, and enzymatically.
Amino groups can be modified by acylation with various activated carbonyl compounds. Among these compounds, activated N-hydroxysuccinimide esters are most suitable for biolabeling and bioconjugation applications.
Compared to other activated esters, NHS derivatives possess good stability in aqueous environment. Optimal pH for the coupling of amines with NHS esters in aqueous buffers is 8.3-8.5. Labeling is also possible in organic solvents. Polar aprotic solents, such as DMF or DMSO, are preferred. One equivalent of tertiary amine base, such as triethylamine, or diisopropylethylamine (DIPEA) is necessary to achieve efficient amine acylation in non-aqueous solution.
AF 488 antibody labeling kit
Ready-to-use kit for labeling antibodies and other proteins with AF 488 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1821-10rxn |
10 rxn
|
$450.00
|
in stock |
AF 594 antibody labeling kit
Ready-to-use kit for labeling antibodies and other proteins with AF 594 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 6821-10rxn |
10 rxn
|
$450.00
|
in stock |
sulfo-Cyanine3 antibody labeling kit
Ready to use kit for the labeling of antibodies (other proteins can be labeled as well) with sulfo-Cyanine3 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1321-10rxn |
10 rxn
|
$450.00
|
in stock |
sulfo-Cyanine5 antibody labeling kit
A kit for the labeling of antibodies with sulfo-Cyanine5 dye, a fluorophore for Cy5 channel.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3321-10rxn |
10 rxn
|
$400.00
|
in stock |
sulfo-Cyanine5.5 antibody labeling kit
A kit for the labeling of antibodies with sulfo-Cyanine5.5 dye, a fluorophore for Cy5.5 channel.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 7321-10rxn |
10 rxn
|
$450.00
|
in stock |
sulfo-Cyanine7 antibody labeling kit
A kit for the labeling of antibodies with sulfo-Cyanine7 dye, a fluorophore for Cy7 channel.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 5321-10rxn |
10 rxn
|
$450.00
|
in stock |
sulfo-Cyanine7.5 antibody labeling kit
A kit for the labeling of antibodies with sulfo-Cyanine7.5 dye, a fluorophore for Cy7.5 channel.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 6321-10rxn |
10 rxn
|
$450.00
|
in stock |
(5,6)-FAM diacetate NHS ester
Amine-reactive fluorescein (FAM) derivative, a mixture of 5- and 6-isomers. The reagent becomes brightly green fluorescent once it is hydrolyzed while diffusing into cells. A fluorescent tracer.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2934-25mg | 25 mg | $160.00 | in stock | |
| 2934-50mg | 50 mg | $270.00 | in stock | |
| 2934-100mg | 100 mg |
$520.00
|
in stock |
AF 430 NHS ester
AF 430 is a hydrophilic coumarin dye with emission in the green-yellow range. The NHS ester is used for the labeling of amine groups.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 10820 | 1 mg | $110.00 | in stock | |
| 20820 | 5 mg | $165.00 | in stock | |
| 40820 | 25 mg | $380.00 | in stock | |
| 50820 | 50 mg |
$695.00
|
in stock | |
| 60820 | 100 mg |
$1190.00
|
in stock |
AF 488 NHS ester
AF 488 NHS ester, a bright, photostable, and hydrophilic fluorescent dye for the green channel.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11820 | 1 mg | $110.00 | in stock | |
| 21820 | 5 mg | $290.00 | in stock | |
| 31820 | 10 mg |
$470.00
|
in stock | |
| 41820 | 25 mg |
$690.00
|
in stock | |
| 51820 | 50 mg |
$1270.00
|
in stock | |
| 61820 | 100 mg |
$1990.00
|
in stock |
AF 555 NHS ester
AF 555 NHS ester, a bright, photostable, and hydrophilic fluorescent dye for the yellow channel.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1K820 | 1 mg | $120.00 | in stock | |
| 2K820 | 5 mg |
$400.00
|
in stock | |
| 4K820 | 25 mg |
$1210.00
|
in stock | |
| 5K820 | 50 mg |
$1710.00
|
12 days | |
| 6K820 | 100 mg |
$2425.00
|
in stock |
AF 568 NHS ester
AF 568 derivative for labeling proteins and other high- and low-molecular weight ligands with free amines.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 14820 | 1 mg | $110.00 | 5 days | |
| 24820 | 5 mg | $290.00 | 5 days | |
| 44820 | 25 mg |
$690.00
|
in stock | |
| 54820 | 50 mg |
$1270.00
|
in stock | |
| 64820 | 100 mg |
$1990.00
|
in stock |
AF 594 NHS ester, 5-isomer
Red-fluorescent NHS-ester of AF 594 dye for labeling proteins, peptides, antibodies, and any molecules containing an -NH2 group
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2825-1mg | 1 mg | $120.00 | in stock | |
| 2825-5mg | 5 mg | $355.00 | in stock | |
| 2825-25mg | 25 mg |
$1150.00
|
in stock | |
| 2825-50mg | 50 mg |
$1955.00
|
in stock | |
| 2825-100mg | 100 mg |
$2425.00
|
in stock |
AF 647 NHS ester
Bright, water-soluble succinimide ester of AF 647 dye for protein and antibody labeling.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 16820 | 1 mg | $110.00 | in stock | |
| 26820 | 5 mg | $340.00 | in stock | |
| 46820 | 25 mg |
$1100.00
|
in stock | |
| 56820 | 50 mg |
$1890.00
|
in stock | |
| 66820 | 100 mg |
$2990.00
|
in stock |
Alkyne-PEG2-NHS ester
Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on diethyleneglycol (PEG2).
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2219-1g | 1 g |
$490.00
|
in stock | |
| 2219-5g | 5 g |
$980.00
|
21 days |
Alkyne-PEG3-NHS ester
Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on triethyleneglycol (PEG3).
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2298-1g | 1 g |
$490.00
|
in stock | |
| 2298-5g | 5 g |
$980.00
|
21 days | |
| 2298-25g | 25 g | please inquire | 21 days |
Alkyne-PEG4-NHS ester
Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on tetraethyleneglycol (PEG4).
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2409-1g | 1 g |
$790.00
|
21 days |
AMCA NHS ester
NHS-ester of AMCA dye for proteins, peptides, and modified oligonucleotides labeling via reaction with amino groups. AMCA is one of the brightest blue fluorescent dyes with large Stoke's shift and high resistance to photobleaching.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3606-5mg | 5 mg | $110.00 | in stock | |
| 3606-25mg | 25 mg | $290.00 | in stock | |
| 3606-50mg | 50 mg |
$435.00
|
in stock | |
| 3606-100mg | 100 mg |
$595.00
|
in stock |
Azide-PEG6-NHS ester
A hydrophilic bifunctional linker with azide and NHS ester function groups flanking PEG6 (hexaethylene glycol).
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3692-250mg | 250 mg | $325.00 | 12 days | |
| 3692-1g | 1 g |
$539.00
|
in stock |
Azidobutyric acid NHS ester
Activated NHS ester for azido-labeling of peptides and proteins.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 53720 | 50 mg | $60.00 | in stock | |
| 63720 | 100 mg | $95.00 | in stock | |
| 73720 | 1 g | please inquire | in stock | |
| 83720 | 5 g | please inquire | in stock |
BDP 558/568 NHS ester
BDP 558/568 is a fluorophore for the yellow region of the spectrum. Like other BDP dyes, this fluorophore has high fluorescence quantum yield, brightness, and photostability.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 17420 | 1 mg | $110.00 | in stock | |
| 27420 | 5 mg | $210.00 | in stock | |
| 47420 | 25 mg |
$410.00
|
in stock | |
| 57420 | 50 mg |
$695.00
|
in stock | |
| 67420 | 100 mg |
$1190.00
|
in stock |
BDP 564/570 NHS ester
Lypohilic borodipyrromethene dye with orange emission for the labeling of primary and secondary amine groups.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1H420 | 1 mg | $110.00 | in stock | |
| 2H420 | 5 mg | $210.00 | in stock | |
| 4H420 | 25 mg |
$410.00
|
in stock | |
| 5H420 | 50 mg |
$695.00
|
in stock | |
| 6H420 | 100 mg |
$1190.00
|
in stock |
BDP 576/589 NHS ester
BDP 576/589 NHS ester (BODIPY™ 576/589 analog) is a bright hydrophobic dye with emission in the orange spectrum range. It can be used in fluorescence anisotropy assays.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1G420 | 1 mg | $110.00 | in stock | |
| 2G420 | 5 mg | $210.00 | in stock | |
| 4G420 | 25 mg |
$410.00
|
in stock | |
| 5G420 | 50 mg |
$695.00
|
in stock | |
| 6G420 | 100 mg |
$1190.00
|
in stock |
BDP 581/591 NHS ester
NHS ester derivative of BDP 581/591 borondipyrromethene fluorophore for the labeling of proteins, peptides, and other biomolecules. The dye is useful for fluorescence polarization and two-photon experiments, and as a ROS probe.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 16420 | 1 mg | $110.00 | in stock | |
| 26420 | 5 mg | $210.00 | in stock | |
| 46420 | 25 mg |
$410.00
|
in stock | |
| 56420 | 50 mg |
$695.00
|
in stock | |
| 66420 | 100 mg |
$1190.00
|
in stock |
BDP 630/650 X NHS ester
BDP 630/650 is a borondipyrromethene fluorophore tuned to Cyanine5 excitation and emission channels. The dye is bright and highly photostable. It is useful for fluorescence polarization assays This is an amine-reactive NHS ester derivative.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 15420 | 1 mg | $110.00 | in stock | |
| 25420 | 5 mg | $210.00 | in stock | |
| 45420 | 25 mg |
$410.00
|
in stock | |
| 55420 | 50 mg |
$695.00
|
in stock | |
| 65420 | 100 mg |
$1190.00
|
in stock |