Primary or secondary amino groups are ubiquitous in biomolecules. They are present in proteins and peptides as ε-amino groups of lysines and N-terminal amino groups. Many natural and synthetic small molecules are amines, and DNAs with amino groups can be prepared by solid phase synthesis, and enzymatically.
Amino groups can be modified by acylation with various activated carbonyl compounds. Among these compounds, activated N-hydroxysuccinimide esters are most suitable for biolabeling and bioconjugation applications.
Compared to other activated esters, NHS derivatives possess good stability in aqueous environment. Optimal pH for the coupling of amines with NHS esters in aqueous buffers is 8.3-8.5. Labeling is also possible in organic solvents. Polar aprotic solents, such as DMF or DMSO, are preferred. One equivalent of tertiary amine base, such as triethylamine, or diisopropylethylamine (DIPEA) is necessary to achieve efficient amine acylation in non-aqueous solution.
Amine-reactive fluorescein (FAM) derivative, a mixture of 5- and 6-isomers. The reagent becomes brightly green fluorescent once it is hydrolyzed while diffusing into cells. A fluorescent tracer.
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2934-25mg
25 mg
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Bifunctional crosslinker with an amine-reactive succinimidyl ester and a thiol-reactive maleimide group.
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3419-500mg
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AF 343 (Coumarin) is a blue emitting dye that can serve as a FRET donor for fluorescein (FAM). This structure features a linker between Coumarin 343 fluorophore, and the functional group. It provides better solubility, and spatial separation between the dye, and the functional group.
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52620
50 mg
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AF 430 is a hydrophilic coumarin dye with emission in the green-yellow range. The NHS ester is used for the labeling of amine groups.
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50820
50 mg
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AF 488 NHS ester, a bright, photostable, and hydrophilic fluorescent dye for the green channel.
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NHS-ester of AF 532 dye for protein, peptide, and modified oligonucleotide labeling via reaction with amino groups.
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1C820
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AF 555 NHS ester, a bright, photostable, and hydrophilic fluorescent dye for the yellow channel.
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1K820
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AF 568 derivative for labeling proteins and other high- and low-molecular weight ligands with free amines.
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54820
50 mg
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Red-fluorescent NHS-ester of AF 594 dye for labeling proteins, peptides, antibodies, and any molecules containing an -NH2 group. Pure 5-isomer
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2825-1mg
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2825-25mg
25 mg
$1240.00
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Bright, water-soluble succinimide ester of AF 647 dye for protein and antibody labeling.
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16820
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5 mg
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46820
25 mg
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56820
50 mg
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100 mg
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Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on diethyleneglycol (PEG2).
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2219-1g
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Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on triethyleneglycol (PEG3).
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2298-1g
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Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on tetraethyleneglycol (PEG4).
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2409-100mg
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NHS-ester of AMCA dye for protein, peptide, and modified oligonucleotide labeling via reaction with amino groups. AMCA is one of the brightest blue fluorescent dyes with large Stoke's shift and high resistance to photobleaching.
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3606-5mg
5 mg
$110.00
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25 mg
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3606-50mg
50 mg
$435.00
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100 mg
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A hydrophilic bifunctional linker with azide and NHS ester function groups flanking PEG6 (hexaethylene glycol).
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3692-250mg
250 mg
$325.00
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1 g
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Activated NHS ester for azido-labeling of peptides and proteins.
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53720
50 mg
$110.00
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63720
100 mg
$210.00
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73720
1 g
$950.00
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83720
5 g
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BDP 558/568 is a fluorophore for the yellow region of the spectrum. Like other BDP dyes, this fluorophore has high fluorescence quantum yield, brightness, and photostability.
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17420
1 mg
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5 mg
$260.00
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47420
25 mg
$510.00
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57420
50 mg
$895.00
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67420
100 mg
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Lypohilic borodipyrromethene dye with orange emission for the labeling of primary and secondary amine groups.
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1H420
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25 mg
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50 mg
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BDP 576/589 NHS ester is a bright hydrophobic dye with emission in the orange spectrum range. It can be used in fluorescence anisotropy assays.
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1G420
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5 mg
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25 mg
$510.00
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50 mg
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NHS ester derivative of BDP 581/591 borondipyrromethene fluorophore for the labeling of proteins, peptides, and other biomolecules. The dye is useful for fluorescence polarization and two-photon experiments, and as a ROS probe.
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5 mg
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46420
25 mg
$510.00
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56420
50 mg
$895.00
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100 mg
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BDP 630/650 is a borondipyrromethene fluorophore tuned to Cyanine5 excitation and emission channels. The dye is bright and highly photostable. It is useful for fluorescence polarization assays. This is an amine-reactive NHS ester derivative.
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15420
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5 mg
$260.00
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45420
25 mg
$510.00
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55420
50 mg
$895.00
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65420
100 mg
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Amine reactive of BDP 650/665, a borondipyrromethene dye for Cy5 channel, possessing a high molar extinction coefficient, and a good emission quantum yield.
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1E420
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25 mg
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Amino-reactive BDP FL, a very bright and photostable dye for fluorescein channel.
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11420
1 mg
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5 mg
$260.00
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25 mg
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51420
50 mg
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61420
100 mg
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BDP R6G is a borondipyrromethene dye which has absorption and emission spectra similar to R6G.
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54420
50 mg
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Amine reactive NHS ester of BDP TMR, bright borondipyrromethene dye for TAMRA channel especially suitable for fluorescence polarization assays.
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5 mg
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52420
50 mg
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Amine reactive NHS ester of BDP TMR, bright borondipyrromethene dye for TAMRA channel especially suitable for fluorescence polarization assays. This compound contains an aminohexanoic acid (C6) linker between the dye and the functional group.
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1F420
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25 mg
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BDP TR is a borondipyrromethene dye. A bright and photostable fluorophore with high quantum yield.
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BDP TR X NHS ester is a derivative of BDP TR containing a long linker based on aminohexanoic acid (C6). The dye has absorption and emission close to ROX. The NHS ester function can be used to conjugate proteins and peptides.
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Biotin derivative with C6-spacer to reduce steric effect when binding amino acids, peptides, or proteins by reacting with primary amines.
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2551-100mg
100 mg
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PEG4-containing activated ester for simple and efficient biotin labeling of antibodies, proteins, and other primary amine-containing biomolecules.
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2893-100mg
100 mg
$145.00
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Cyanine2 NHS ester minimal dye for protein labeling
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1A041
5 nmol
$110.00
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10 nmol
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25 nmol
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Amine-reactive Cyanine3 dye NHS ester for the labeling of amino-groups.
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Cyanine3 minimal dye for protein labeling.
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1B041
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$110.00
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Cyanine3.5 dye NHS ester derivative the labeling of amino-groups in biomolecules.
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12020
1 mg
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5 mg
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42020
25 mg
$510.00
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50 mg
$895.00
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100 mg
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Cyanine3B NHS ester is a reactive dye with significantly increased fluorescence quantum yield and photostability for the specific labeling of amino-groups in biomolecules.
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19320
1 mg
$140.00
5 days
29320
5 mg
$340.00
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49320
25 mg
$990.00
in stock
59320
50 mg
$1690.00
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69320
100 mg
$3100.00
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Amine-reactive Cyanine5 activated ester for the labeling of proteins, peptides, and other molecules.
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Cy5 NHS ester minimal dye for protein labeling.
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1C041
5 nmol
$110.00
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2C041
10 nmol
$175.00
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3C041
25 nmol
$265.00
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Cyanine5.5 NHS is far-red / near-infrared amine-reactive dye.
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17020
1 mg
$125.00
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27020
5 mg
$260.00
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47020
25 mg
$510.00
in stock
57020
50 mg
$895.00
in stock
67020
100 mg
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Amine reactive NHS ester of near infrared dye Cyanine7.
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15020
1 mg
$125.00
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25020
5 mg
$260.00
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45020
25 mg
$510.00
in stock
55020
50 mg
$895.00
in stock
65020
100 mg
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Amine reactive NHS ester of NIR fluorescent dye Cyanine7.5 .
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16020
1 mg
$125.00
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26020
5 mg
$260.00
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46020
25 mg
$510.00
in stock
56020
50 mg
$895.00
in stock
66020
100 mg
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Azodibenzocyclooctyne (DBCO, ADIBO) reagent for strain-promoted copper-free click chemistry (SPAAC). The reagent contains an NHS ester function for the attachment of the cyclooctyne to various molecules.
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A heterobifunctional linker with DBCO and NHS ester function groups flanking PEG6 (hexaethylene glycol).
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3664-50mg
50 mg
$247.00
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3664-100mg
100 mg
$449.00
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3664-1g
1 g
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Homobifunctional crosslinker with two NHS ester groups at each end of a short 5-atom (7.7 angstrom) spacer.
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4018-100mg
100 mg
$95.00
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4018-1g
1 g
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Bifunctional linker with DOTA chelating and amine-reactive NHS ester groups for bioconjugation.
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4245-100mg
100 mg
$125.00
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4245-500mg
500 mg
$525.00
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4245-1g
1 g
$990.00
21 days
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Functionalized DusQ 1 quencher derivative with quenching range from 480 - 580 nm for labeling of amine-containing molecules.
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41D20
25 mg
$315.00
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51D20
50 mg
$580.00
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61D20
100 mg
$885.00
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DusQ 2 dark quencher with a quenching range from 560 - 670 nm with primary amine reactivity for synthesis of FRET probes. This NHS ester can be coupled to various molecules bearing amino groups.
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42D20
25 mg
$315.00
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52D20
50 mg
$580.00
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62D20
100 mg
$885.00
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Amino-reactive NHS ester with quencher for the conjugation with amines-containing molecules, DusQ 21 quenching range 590 - 720 nm.
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48D20
25 mg
$315.00
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Amino-reactive NHS ester for the labeling with far-red quencher DusQ 3.
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43D20
25 mg
$315.00
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53D20
50 mg
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63D20
100 mg
$885.00
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Activated N-hydroxysuccinimide ester of fluorescein (FAM), pure 6-isomer.
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NHS-ester of HEX dye for proteins, peptides, and modified oligonucleotides labeling via reaction with amino groups.
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2B220
5 mg
$175.00
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Hexynoic acid NHS ester for the labeling of biomolecules with alkyne group for copper-catalyzed click chemistry.
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41720
25 mg
$95.00
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51720
50 mg
$180.00
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61720
100 mg
$290.00
in stock
91720
1 g
please inquire
in stock
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JOE is a fluorescent dye with emission in the yellow part of the spectrum. This derivative is an activated NHS ester for conjugation with amines.
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11120
1 mg
$110.00
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21120
5 mg
$210.00
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41120
25 mg
$410.00
in stock
51120
50 mg
$695.00
in stock
61120
100 mg
$1190.00
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Pyrene NHS ester for the labeling of biomolecules with pyrene residue. Pyrene is a fluorescent proximity probe which forms excimers, and it is also an anchor group for graphite immobilization.
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63520
100 mg
$110.00
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R6G (rhodamine 6G) NHS ester, pure 5-isomer. This dye is often used for the labeling of oligonucleotides for qPCR.
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14220
1 mg
$110.00
in stock
24220
5 mg
$210.00
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44220
25 mg
$410.00
in stock
54220
50 mg
$695.00
in stock
64220
100 mg
$1190.00
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Activated NHS ester of ROX (rhodamine X or rhodamine 101), pure 5-isomer, for the labeling of amine groups of biomolecules.
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11220
1 mg
$110.00
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21220
5 mg
$210.00
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41220
25 mg
$410.00
in stock
51220
50 mg
$695.00
in stock
61220
100 mg
$1190.00
5 days
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Pure 6-isomer of ROX (Rhodamine X, R101) NHS-ester for amine labeling.
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12220
1 mg
$110.00
in stock
22220
5 mg
$210.00
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42220
25 mg
$410.00
in stock
52220
50 mg
$695.00
in stock
62220
100 mg
$1190.00
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Water soluble sulfo-Cyanine3 SE activated ester for amino-biomolecule labeling.
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11320
1 mg
$91.00
in stock
21320
5 mg
$119.00
in stock
41320
25 mg
$395.00
in stock
51320
50 mg
$729.00
in stock
61320
100 mg
$1290.00
in stock
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Amine reactive activated ester of water soluble, sulfonated sulfo-Cyanine3.5 dye.
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12320
1 mg
$125.00
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22320
5 mg
$325.00
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42320
25 mg
$850.00
in stock
52320
50 mg
$1490.00
in stock
62320
100 mg
$1990.00
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Water soluble amine reactive Cyanine5 dye with two NHS ester groups.
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133D0
1 mg
$125.00
5 days
233D0
5 mg
$325.00
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433D0
25 mg
$850.00
in stock
533D0
50 mg
$1490.00
in stock
633D0
100 mg
$1990.00
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Water-soluble sulfo-Cyanine5 succinimidyl ester for labeling various amine-containing molecules in the aqueous phase.
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13320
1 mg
$91.00
in stock
23320
5 mg
$119.00
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43320
25 mg
$345.00
in stock
53320
50 mg
$557.00
in stock
63320
100 mg
$890.00
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Water soluble, sulfonated, far-red sulfo-Cyanine5.5 dye in the form of NHS ester.
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17320
1 mg
$125.00
in stock
27320
5 mg
$325.00
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47320
25 mg
$850.00
in stock
57320
50 mg
$1490.00
in stock
67320
100 mg
$1990.00
in stock
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sulfo-Cyanine7 bis-NHS ester is a bifunctional derivative of water-soluble near-infrared sulfo-Cyanine7. It contains two activated ester functional groups.
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153D0
1 mg
$125.00
in stock
253D0
5 mg
$325.00
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453D0
25 mg
$850.00
in stock
553D0
50 mg
$1490.00
in stock
653D0
100 mg
$1990.00
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sulfo-Cyanine7 NHS ester is a water-soluble, near-infrared, amine-reactive dye.
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15320
1 mg
$125.00
in stock
25320
5 mg
$325.00
in stock
45320
25 mg
$850.00
in stock
55320
50 mg
$1490.00
in stock
65320
100 mg
$1990.00
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A water-soluble near-infrared emitting dye for in vivo imaging applications. sulfo-Cyanine7.5 is an analog of indocyanine green (ICG) possessing higher emission quantum yield and a linker arm.
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16320
1 mg
$125.00
in stock
26320
5 mg
$325.00
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46320
25 mg
$850.00
in stock
56320
50 mg
$1490.00
in stock
66320
100 mg
$1990.00
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Amine-reactive, activated N-hydroxysuccinimidyl ester of TAMRA dye for the labeling of amine groups of proteins, peptides, and amino-oligonucleotides. Pure 5-isomer.
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27120
5 mg
$210.00
in stock
47120
25 mg
$350.00
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57120
50 mg
$595.00
in stock
67120
100 mg
$790.00
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NHS-ester of TAMRA dye for proteins, peptides, and modified oligonucleotides labeling via reaction with amino groups. Pure 6-isomer.
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18120
1 mg
$110.00
in stock
28120
5 mg
$210.00
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48120
25 mg
$350.00
in stock
58120
50 mg
$595.00
in stock
68120
100 mg
$790.00
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A heterobifunctional linker with trans-cyclooctene and NHS ester groups flanking PEG4 (tetraethylene glycol) for labeling of antibodies, proteins, and other primary amine-containing biomolecules.
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3056-10mg
10 mg
$145.00
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3056-25mg
25 mg
$225.00
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3056-100mg
100 mg
$550.00
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Thiazole Orange NHS ester for labeling biomolecules (peptides, proteins, amino-DNA, etc.). Thiazole orange is a cyanine dye that is essentially dark in solution but has a bright fluorescence in double-stranded DNA and RNA.
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2183-5mg
5 mg
$60.00
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TR (aka Texas Red) is a red-emitting fluorescent dye based on sulforhodamine scaffold, an amine-reactive NHS ester.
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1V220
1 mg
$125.00
in stock
2V220
5 mg
$325.00
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4V220
25 mg
$510.00
in stock
5V220
50 mg
$895.00
in stock
6V220
100 mg
$1490.00
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