NHS esters in Reactive groups
Primary or secondary amino groups are ubiquitous in biomolecules. They are present in proteins and peptides as ε-amino groups of lysines and N-terminal amino groups. Many natural and synthetic small molecules are amines, and DNAs with amino groups can be prepared by solid phase synthesis, and enzymatically.
Amino groups can be modified by acylation with various activated carbonyl compounds. Among these compounds, activated N-hydroxysuccinimide esters are most suitable for biolabeling and bioconjugation applications.
Compared to other activated esters, NHS derivatives possess good stability in aqueous environment. Optimal pH for the coupling of amines with NHS esters in aqueous buffers is 8.3-8.5. Labeling is also possible in organic solvents. Polar aprotic solents, such as DMF or DMSO, are preferred. One equivalent of tertiary amine base, such as triethylamine, or diisopropylethylamine (DIPEA) is necessary to achieve efficient amine acylation in non-aqueous solution.
Alkyne NHS ester (hexynoic acid NHS ester)
Alkyne NHS ester for the labeling of biomolecules with alkyne group for Copper catalyzed Click chemistry.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 41720 | 25 mg | $95.00 | stock | |
| 51720 | 50 mg | $195.00 | stock | |
| 61720 | 100 mg | $290.00 | stock |
Azidobutyric acid NHS ester
Activated ester for azido labeling of peptides and proteins.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 33720 | 10 mg | $60.00 | stock | |
| 43720 | 25 mg | $95.00 | stock | |
| 53720 | 50 mg | $195.00 | stock | |
| 63720 | 100 mg | $290.00 | stock |
BDP 558/568 NHS ester
BDP 558/568 is a fluorophore for the yellow region of the spectrum. Like other BDP dyes, this fluorophore has high fluorescence quantum yield, brightness, and photostability.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 17420 | 1 mg | $110.00 | stock | |
| 27420 | 5 mg | $210.00 | stock | |
| 47420 | 25 mg |
$410.00 |
stock | |
| 57420 | 50 mg |
$695.00 |
stock | |
| 67420 | 100 mg |
$1190.00 |
stock |
BDP 581/591 NHS ester
NHS ester derivative of BDP 581/591 borondipyrromethene fluorophore for the labeling of proteins, peptides, and other biomolecules. The dye is useful for fluorescence polarization and two-photon experiments, and as a ROS probe.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 16420 | 1 mg | $110.00 | stock | |
| 26420 | 5 mg | $210.00 | stock | |
| 46420 | 25 mg |
$410.00 |
stock | |
| 56420 | 50 mg |
$695.00 |
stock | |
| 66420 | 100 mg |
$1190.00 |
stock |
BDP 630/650 X NHS ester
BDP 630/650 is a borondipyrromethene fluorophore tuned to Cyanine5 excitation and emission channels. The dye is bright and highly photostable. It is useful for fluorescence polarization assays This is an amine-reactive NHS ester derivative.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 15420 | 1 mg | $110.00 | stock | |
| 25420 | 5 mg | $210.00 | stock | |
| 45420 | 25 mg |
$410.00 |
stock | |
| 55420 | 50 mg |
$695.00 |
stock | |
| 65420 | 100 mg |
$1190.00 |
stock |
BDP FL NHS ester
Amino-reactive BDP FL, a very bright and photostable dye for fluorescein channel.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11420 | 1 mg | $110.00 | stock | |
| 21420 | 5 mg | $210.00 | stock | |
| 41420 | 25 mg |
$410.00 |
stock | |
| 51420 | 50 mg |
$695.00 |
stock | |
| 61420 | 100 mg |
$1190.00 |
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BDP R6G NHS ester
BDP R6G is a borondipyrromethene dye which has absorption and emission spectra similar to R6G.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 14420 | 1 mg | $110.00 | stock | |
| 24420 | 5 mg | $210.00 | stock | |
| 44420 | 25 mg |
$410.00 |
stock | |
| 54420 | 50 mg |
$695.00 |
stock | |
| 64420 | 100 mg |
$1190.00 |
stock |
BDP TMR NHS ester
Amine reactive NHS ester of BDP TMR, bright borondipyrromethene dye for TAMRA channel especially suitable for fluorescence polarization assays.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 12420 | 1 mg | $110.00 | stock | |
| 22420 | 5 mg | $210.00 | stock | |
| 42420 | 25 mg |
$410.00 |
stock | |
| 52420 | 50 mg |
$695.00 |
stock | |
| 62420 | 100 mg |
$1190.00 |
stock |
BDP TR NHS ester
BDP TR is a borondipyrromethene dye for Texas Red channel. A bright and photostable fluorophore with high quantum yield.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 13420 | 1 mg | $110.00 | stock | |
| 23420 | 5 mg | $210.00 | stock | |
| 43420 | 25 mg |
$410.00 |
stock | |
| 53420 | 50 mg |
$695.00 |
stock | |
| 63420 | 100 mg |
$1190.00 |
stock |
Coumarin 343 X NHS ester
Coumarin 343 is a blue emitting dye that can serve as a FRET donor for fluorescein (FAM). This structure features a linker between Coumarin 343 fluorophore, and the functional group. It provides better solubility, and spatial separation between the dye, and the functional group.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 12620 | 1 mg | $110.00 | stock | |
| 22620 | 5 mg | $210.00 | stock | |
| 42620 | 25 mg |
$410.00 |
stock | |
| 52620 | 50 mg |
$695.00 |
stock | |
| 62620 | 100 mg |
$1190.00 |
stock |
Cyanine2 NHS ester minimal dye
Cyanine2 NHS ester minimal dye for protein labeling
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1A041 | 5 nmol | $85.00 | stock | |
| 2A041 | 10 nmol | $140.00 | stock | |
| 3A041 | 25 nmol | $210.00 | stock |
Cyanine3 NHS ester
Amine-reactive Cyanine3 dye NHS ester.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11020 | 1 mg | $110.00 | stock | |
| 21020 | 5 mg | $210.00 | stock | |
| 41020 | 25 mg |
$410.00 |
stock | |
| 51020 | 50 mg |
$695.00 |
stock | |
| 61020 | 100 mg |
$1190.00 |
stock |
Cyanine3 NHS ester minimal dye
Cyanine3 minimal dye for protein labeling.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1B041 | 5 nmol | $85.00 | 5 days | |
| 2B041 | 10 nmol | $140.00 | stock | |
| 3B041 | 25 nmol | $210.00 | stock |
Cyanine3.5 NHS ester
Cyanine3.5 dye NHS ester derivative.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 12020 | 1 mg | $110.00 | stock | |
| 22020 | 5 mg | $210.00 | stock | |
| 42020 | 25 mg |
$410.00 |
5 days | |
| 52020 | 50 mg |
$695.00 |
stock | |
| 62020 | 100 mg |
$1190.00 |
stock |
Cyanine5 NHS ester
Amine-reactive Cyanine5 activated ester for the labeling of proteins, peptides, and other molecules.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 13020 | 1 mg | $110.00 | stock | |
| 23020 | 5 mg | $210.00 | stock | |
| 43020 | 25 mg |
$410.00 |
stock | |
| 53020 | 50 mg |
$695.00 |
stock | |
| 63020 | 100 mg |
$1190.00 |
stock |
Cyanine5 NHS ester minimal dye
Cy5 NHS ester minimal dye for protein labeling.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1C041 | 5 nmol | $85.00 | stock | |
| 2C041 | 10 nmol | $140.00 | stock | |
| 3C041 | 25 nmol | $210.00 | stock |
Cyanine5.5 NHS ester
Cyanine5.5 NHS is far-red / near-infrared amine-reactive dye.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 17020 | 1 mg | $110.00 | stock | |
| 27020 | 5 mg | $210.00 | stock | |
| 47020 | 25 mg |
$410.00 |
stock | |
| 57020 | 50 mg |
$695.00 |
stock | |
| 67020 | 100 mg |
$1190.00 |
stock |
Cyanine7 NHS ester
Amine reactive NHS ester of near infrared dye Cyanine7.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 15020 | 1 mg | $110.00 | stock | |
| 25020 | 5 mg | $210.00 | stock | |
| 45020 | 25 mg |
$410.00 |
stock | |
| 55020 | 50 mg |
$695.00 |
stock | |
| 65020 | 100 mg |
$1190.00 |
stock |
Cyanine7.5 NHS ester
Amine reactive NHS ester of NIR fluorescent dye Cyanine7.5.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 16020 | 1 mg | $110.00 | stock | |
| 26020 | 5 mg | $210.00 | stock | |
| 46020 | 25 mg |
$410.00 |
stock | |
| 56020 | 50 mg |
$695.00 |
stock | |
| 66020 | 100 mg |
$1190.00 |
stock |
DBCO NHS ester
Azodibenzocyclooctyne (DBCO, ADIBO) reagent for strain promoted copper free Click chemistry (spAAC). The reagent contains an NHS ester function for the attachment of the cyclooctyne to various molecules.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 34720 | 10 mg | $60.00 | stock | |
| 44720 | 25 mg | $95.00 | stock | |
| 54720 | 50 mg | $145.00 | stock | |
| 64720 | 100 mg | $210.00 | stock | |
| 94720 | 1 g |
$1190.00 |
stock |
FAM NHS ester, 6-isomer
Activated N-hydroxysuccinimide ester of fluorescein (FAM), pure 6-isomer.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 15120 | 1 mg | $110.00 | stock | |
| 25120 | 5 mg | $210.00 | stock | |
| 45120 | 25 mg | $160.00 | stock | |
| 55120 | 50 mg | $270.00 | stock | |
| 65120 | 100 mg |
$420.00 |
5 days |
Pyrenebutyric acid NHS ester
Pyrene NHS ester for the labeling of biomolecules with pyrene residue. Pyrene is a fluorescent proximity probe which forms excimers, and it is also an anchor group for graphite immobilization.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 63520 | 100 mg | $110.00 | stock |
ROX NHS ester, pure 6- isomer
Pure isomer 6-ROX NHS ester for amine labeling.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 12220 | 1 mg | $110.00 | stock | |
| 22220 | 5 mg | $210.00 | 5 days | |
| 42220 | 25 mg |
$410.00 |
21 days | |
| 52220 | 50 mg |
$695.00 |
stock | |
| 62220 | 100 mg |
$1190.00 |
stock |
Sulfo-Cyanine3 NHS ester
Water soluble sulfo-Cyanine3 SE activated ester for amino-biomolecule labeling.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11320 | 1 mg | $110.00 | stock | |
| 21320 | 5 mg | $290.00 | stock | |
| 41320 | 25 mg |
$690.00 |
stock | |
| 51320 | 50 mg |
$1270.00 |
stock | |
| 61320 | 100 mg |
$1990.00 |
stock |
Sulfo-Cyanine5 bis-NHS ester
Water soluble amine reactive Cyanine5 dye with two NHS ester groups.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 133D0 | 1 mg | $110.00 | stock | |
| 233D0 | 5 mg | $290.00 | stock | |
| 433D0 | 25 mg |
$690.00 |
stock | |
| 533D0 | 50 mg |
$1270.00 |
stock | |
| 633D0 | 100 mg |
$1990.00 |
stock |
Sulfo-Cyanine5 NHS ester
Water-soluble Cyanine 5 succinimide ester.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 13320 | 1 mg | $110.00 | stock | |
| 23320 | 5 mg | $290.00 | stock | |
| 43320 | 25 mg |
$690.00 |
stock | |
| 53320 | 50 mg |
$1270.00 |
stock | |
| 63320 | 100 mg |
$1990.00 |
stock |
Sulfo-Cyanine5.5 NHS ester
Water soluble, sulfonated, far-red sulfo-Cyanine5.5 dye in the form of NHS ester.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 17320 | 1 mg | $110.00 | stock | |
| 27320 | 5 mg | $290.00 | stock | |
| 47320 | 25 mg |
$690.00 |
stock | |
| 57320 | 50 mg |
$1190.00 |
stock | |
| 67320 | 100 mg |
$1850.00 |
5 days |
Sulfo-Cyanine7 NHS ester
Sulfo-Cyanine7 NHS ester is a water soluble, near infrared, amine-reactive dye.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 15320 | 1 mg | $110.00 | stock | |
| 25320 | 5 mg | $290.00 | stock | |
| 45320 | 25 mg |
$690.00 |
stock | |
| 55320 | 50 mg |
$1270.00 |
stock | |
| 65320 | 100 mg |
$1990.00 |
stock |
sulfo-Cyanine7.5 NHS ester
A water soluble near infrared emitting dye for in vivo imaging applications. Sulfo-Cyanine7.5 is an analog of indocyanine green (ICG) possessing higher emission quantum yield, and containing a linker arm.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 16320 | 1 mg | $110.00 | stock | |
| 26320 | 5 mg | $290.00 | stock | |
| 46320 | 25 mg |
$690.00 |
stock | |
| 56320 | 50 mg |
$1190.00 |
stock | |
| 66320 | 100 mg |
$1850.00 |
stock |
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