NHS esters in Reactive groups
Primary or secondary amino groups are ubiquitous in biomolecules. They are present in proteins and peptides as ε-amino groups of lysines and N-terminal amino groups. Many natural and synthetic small molecules are amines, and DNAs with amino groups can be prepared by solid phase synthesis, and enzymatically.
Amino groups can be modified by acylation with various activated carbonyl compounds. Among these compounds, activated N-hydroxysuccinimide esters are most suitable for biolabeling and bioconjugation applications.
Compared to other activated esters, NHS derivatives possess good stability in aqueous environment. Optimal pH for the coupling of amines with NHS esters in aqueous buffers is 8.3-8.5. Labeling is also possible in organic solvents. Polar aprotic solents, such as DMF or DMSO, are preferred. One equivalent of tertiary amine base, such as triethylamine, or diisopropylethylamine (DIPEA) is necessary to achieve efficient amine acylation in non-aqueous solution.
sulfo-Cyanine3 antibody labeling kit
Ready to use kit for the labeling of antibodies (other proteins can be labeled as well) with sulfo-Cyanine3 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1321-10rxn |
10 rxn
|
$390.00 | in stock |
sulfo-Cyanine5 antibody labeling kit
A kit for the labeling of antibodies with sulfo-Cyanine5 dye, a fluorophore for Cy5 channel.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3321-10rxn |
10 rxn
|
$390.00 | in stock |
sulfo-Cyanine5.5 antibody labeling kit
A kit for the labeling of antibodies with sulfo-Cyanine5.5 dye, a fluorophore for Cy5.5 channel.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 7321-10rxn |
10 rxn
|
$390.00 | in stock |
sulfo-Cyanine7 antibody labeling kit
A kit for the labeling of antibodies with sulfo-Cyanine7 dye, a fluorophore for Cy7 channel.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 5321-10rxn |
10 rxn
|
$390.00 | in stock |
sulfo-Cyanine7.5 antibody labeling kit
A kit for the labeling of antibodies with sulfo-Cyanine7.5 dye, a fluorophore for Cy7.5 channel.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 6321-10rxn |
10 rxn
|
$390.00 | in stock |
AF430 NHS ester
AF430 is a hydrophilic coumarin dye with the emission in green-yellow range. The NHS ester is used for the labeling of amine groups.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 10820 | 1 mg | $110.00 | in stock | |
| 20820 | 5 mg | $165.00 | in stock | |
| 40820 | 25 mg | $380.00 | in stock | |
| 50820 | 50 mg |
$695.00
|
in stock | |
| 60820 | 100 mg |
$1190.00
|
in stock |
AF488 NHS ester
AF488 NHS ester, a bright, photostable and hydrophilic fluorescent dye for green channel.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11820 | 1 mg | $110.00 | in stock | |
| 21820 | 5 mg | $290.00 | in stock | |
| 41820 | 25 mg |
$690.00
|
in stock | |
| 51820 | 50 mg |
$1270.00
|
in stock | |
| 61820 | 100 mg |
$1990.00
|
5 days |
AF568 NHS ester
AF568 derivative for labeling proteins and other high- and low-molecular weight ligands with free amines.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 14820 | 1 mg | $110.00 | in stock | |
| 24820 | 5 mg | $290.00 | in stock | |
| 44820 | 25 mg |
$690.00
|
in stock | |
| 54820 | 50 mg |
$1270.00
|
in stock | |
| 64820 | 100 mg |
$1990.00
|
in stock |
Alkyne NHS ester (hexynoic acid NHS ester)
Alkyne NHS ester for the labeling of biomolecules with alkyne group for Copper catalyzed Click chemistry.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 41720 | 25 mg | $95.00 | in stock | |
| 51720 | 50 mg | $180.00 | in stock | |
| 61720 | 100 mg | $290.00 | in stock | |
| 91720 | 1 g |
please inquire
|
in stock |
Alkyne-PEG3-NHS ester
Bifunctional hyrophilic linker with NHS ester and terminal alkyne functional group based on triethyleneglycol (PEG3).
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2298-1g | 1 g |
$490.00
|
in stock | |
| 2298-5g | 5 g |
$980.00
|
10 days | |
| 2298-25g | 25 g |
please inquire
|
21 days |
Azidobutyric acid NHS ester
Activated ester for azido labeling of peptides and proteins.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 53720 | 50 mg | $60.00 | in stock | |
| 63720 | 100 mg | $95.00 | in stock | |
| 73720 | 1 g |
please inquire
|
in stock | |
| 83720 | 5 g |
please inquire
|
in stock |
BDP 558/568 NHS ester
BDP 558/568 is a fluorophore for the yellow region of the spectrum. Like other BDP dyes, this fluorophore has high fluorescence quantum yield, brightness, and photostability.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 17420 | 1 mg | $110.00 | in stock | |
| 27420 | 5 mg | $210.00 | in stock | |
| 47420 | 25 mg |
$410.00
|
in stock | |
| 57420 | 50 mg |
$695.00
|
in stock | |
| 67420 | 100 mg |
$1190.00
|
in stock |
BDP 564/570 NHS ester
Lypohilic borodipyrromethene dye with orange emission for the labeling of primary and secondary amine groups.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1H420 | 1 mg | $110.00 | in stock | |
| 2H420 | 5 mg | $210.00 | in stock | |
| 4H420 | 25 mg |
$410.00
|
in stock | |
| 5H420 | 50 mg |
$695.00
|
in stock | |
| 6H420 | 100 mg |
$1190.00
|
in stock |
BDP 576/589 NHS ester
BDP 576/589 NHS ester (BODIPY™ 576/589 analog) is a bright hydrophobic dye with emission in the orange spectrum range. It can be used in fluorescence anisotropy assays.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1G420 | 1 mg | $110.00 | in stock | |
| 2G420 | 5 mg | $210.00 | in stock | |
| 4G420 | 25 mg |
$410.00
|
in stock | |
| 5G420 | 50 mg |
$695.00
|
in stock | |
| 6G420 | 100 mg |
$1190.00
|
in stock |
BDP 581/591 NHS ester
NHS ester derivative of BDP 581/591 borondipyrromethene fluorophore for the labeling of proteins, peptides, and other biomolecules. The dye is useful for fluorescence polarization and two-photon experiments, and as a ROS probe.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 16420 | 1 mg | $110.00 | in stock | |
| 26420 | 5 mg | $210.00 | in stock | |
| 46420 | 25 mg |
$410.00
|
in stock | |
| 56420 | 50 mg |
$695.00
|
in stock | |
| 66420 | 100 mg |
$1190.00
|
in stock |
BDP 630/650 X NHS ester
BDP 630/650 is a borondipyrromethene fluorophore tuned to Cyanine5 excitation and emission channels. The dye is bright and highly photostable. It is useful for fluorescence polarization assays This is an amine-reactive NHS ester derivative.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 15420 | 1 mg | $110.00 | in stock | |
| 25420 | 5 mg | $210.00 | in stock | |
| 45420 | 25 mg |
$410.00
|
in stock | |
| 55420 | 50 mg |
$695.00
|
in stock | |
| 65420 | 100 mg |
$1190.00
|
in stock |
BDP 650/665 X NHS ester
Amine reactive of BDP 650/665, a borondipyrromethene dye for Cy5 channel, possessing a high molar extinction coefficient, and a good emission quantum yield.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1E420 | 1 mg | $110.00 | in stock | |
| 2E420 | 5 mg | $210.00 | in stock | |
| 4E420 | 25 mg |
$410.00
|
in stock | |
| 5E420 | 50 mg |
$695.00
|
in stock | |
| 6E420 | 100 mg |
$1190.00
|
in stock |
BDP FL NHS ester
Amino-reactive BDP FL, a very bright and photostable dye for fluorescein channel.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11420 | 1 mg | $110.00 | in stock | |
| 21420 | 5 mg | $210.00 | in stock | |
| 41420 | 25 mg |
$410.00
|
in stock | |
| 51420 | 50 mg |
$695.00
|
in stock | |
| 61420 | 100 mg |
$1190.00
|
5 days |
BDP R6G NHS ester
BDP R6G is a borondipyrromethene dye which has absorption and emission spectra similar to R6G.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 14420 | 1 mg | $110.00 | in stock | |
| 24420 | 5 mg | $210.00 | in stock | |
| 44420 | 25 mg |
$410.00
|
in stock | |
| 54420 | 50 mg |
$695.00
|
in stock | |
| 64420 | 100 mg |
$1190.00
|
in stock |
BDP TMR NHS ester
Amine reactive NHS ester of BDP TMR, bright borondipyrromethene dye for TAMRA channel especially suitable for fluorescence polarization assays.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 12420 | 1 mg | $110.00 | 10 days | |
| 22420 | 5 mg | $210.00 | in stock | |
| 42420 | 25 mg |
$410.00
|
in stock | |
| 52420 | 50 mg |
$695.00
|
in stock | |
| 62420 | 100 mg |
$1190.00
|
in stock |
BDP TR NHS ester
BDP TR is a borondipyrromethene dye. A bright and photostable fluorophore with high quantum yield.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 13420 | 1 mg | $110.00 | in stock (1 pcs) | |
| 23420 | 5 mg | $210.00 | in stock (1 pcs) | |
| 43420 | 25 mg |
$410.00
|
in stock (2 pcs) | |
| 53420 | 50 mg |
$695.00
|
in stock (2 pcs) | |
| 63420 | 100 mg |
$1190.00
|
in stock (4 pcs) |
BDP TR X NHS ester
BDP TR X NHS ester is a derivative of BDP TR containing a long linker based on aminohexanoic acid (C6). The dye has absorption and emission close to ROX. The NHS ester function can be used for the conjugation with proteins and peptides.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1D420 | 1 mg | $110.00 | in stock | |
| 2D420 | 5 mg | $210.00 | in stock | |
| 4D420 | 25 mg |
$410.00
|
in stock | |
| 5D420 | 50 mg |
$695.00
|
in stock | |
| 6D420 | 100 mg |
$1190.00
|
in stock |
Coumarin 343 X NHS ester
Coumarin 343 is a blue emitting dye that can serve as a FRET donor for fluorescein (FAM). This structure features a linker between Coumarin 343 fluorophore, and the functional group. It provides better solubility, and spatial separation between the dye, and the functional group.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 12620 | 1 mg | $110.00 | in stock | |
| 22620 | 5 mg | $210.00 | in stock | |
| 42620 | 25 mg |
$410.00
|
in stock | |
| 52620 | 50 mg |
$695.00
|
in stock | |
| 62620 | 100 mg |
$1190.00
|
in stock |
Cyanine2 NHS ester minimal dye
Cyanine2 NHS ester minimal dye for protein labeling
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1A041 | 5 nmol | $85.00 | in stock | |
| 2A041 | 10 nmol | $140.00 | in stock | |
| 3A041 | 25 nmol | $210.00 | in stock |
Cyanine3 NHS ester
Amine-reactive Cyanine3 dye NHS ester.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11020 | 1 mg | $110.00 | in stock | |
| 21020 | 5 mg | $210.00 | in stock | |
| 41020 | 25 mg |
$410.00
|
in stock | |
| 51020 | 50 mg |
$695.00
|
in stock | |
| 61020 | 100 mg |
$1190.00
|
in stock |
Cyanine3 NHS ester minimal dye
Cyanine3 minimal dye for protein labeling.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1B041 | 5 nmol | $85.00 | in stock | |
| 2B041 | 10 nmol | $140.00 | in stock | |
| 3B041 | 25 nmol | $210.00 | in stock |
Cyanine3.5 NHS ester
Cyanine3.5 dye NHS ester derivative.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 12020 | 1 mg | $110.00 | in stock | |
| 22020 | 5 mg | $210.00 | in stock | |
| 42020 | 25 mg |
$410.00
|
in stock | |
| 52020 | 50 mg |
$695.00
|
in stock | |
| 62020 | 100 mg |
$1190.00
|
in stock |
Cyanine5 NHS ester
Amine-reactive Cyanine5 activated ester for the labeling of proteins, peptides, and other molecules.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 13020 | 1 mg | $110.00 | in stock | |
| 23020 | 5 mg | $210.00 | in stock | |
| 43020 | 25 mg |
$410.00
|
in stock | |
| 53020 | 50 mg |
$695.00
|
in stock | |
| 63020 | 100 mg |
$1190.00
|
in stock |
Cyanine5 NHS ester minimal dye
Cy5 NHS ester minimal dye for protein labeling.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1C041 | 5 nmol | $85.00 | in stock | |
| 2C041 | 10 nmol | $140.00 | in stock | |
| 3C041 | 25 nmol | $210.00 | in stock |
Cyanine5.5 NHS ester
Cyanine5.5 NHS is far-red / near-infrared amine-reactive dye.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 17020 | 1 mg | $110.00 | in stock | |
| 27020 | 5 mg | $210.00 | in stock | |
| 47020 | 25 mg |
$410.00
|
in stock | |
| 57020 | 50 mg |
$695.00
|
in stock | |
| 67020 | 100 mg |
$1190.00
|
in stock |
Cyanine7 NHS ester
Amine reactive NHS ester of near infrared dye Cyanine7.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 15020 | 1 mg | $110.00 | in stock | |
| 25020 | 5 mg | $210.00 | in stock | |
| 45020 | 25 mg |
$410.00
|
in stock | |
| 55020 | 50 mg |
$695.00
|
in stock | |
| 65020 | 100 mg |
$1190.00
|
in stock |
Cyanine7.5 NHS ester
Amine reactive NHS ester of NIR fluorescent dye Cyanine7.5.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 16020 | 1 mg | $110.00 | in stock | |
| 26020 | 5 mg | $210.00 | in stock | |
| 46020 | 25 mg |
$410.00
|
in stock | |
| 56020 | 50 mg |
$695.00
|
in stock | |
| 66020 | 100 mg |
$1190.00
|
in stock |
DBCO NHS ester
Azodibenzocyclooctyne (DBCO, ADIBO) reagent for strain promoted copper free Click chemistry (spAAC). The reagent contains an NHS ester function for the attachment of the cyclooctyne to various molecules.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 34720 | 10 mg | $60.00 | in stock | |
| 44720 | 25 mg | $95.00 | 5 days | |
| 54720 | 50 mg | $145.00 | in stock | |
| 64720 | 100 mg | $210.00 | in stock | |
| 94720 | 1 g |
$1190.00
|
in stock | |
| K4720 | 5 g |
$3570.00
|
in stock | |
| N4720 | 25 g |
please inquire
|
21 days |
FAM NHS ester, 6-isomer
Activated N-hydroxysuccinimide ester of fluorescein (FAM), pure 6-isomer.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 15120 | 1 mg | $110.00 | in stock | |
| 25120 | 5 mg | $160.00 | in stock | |
| 45120 | 25 mg | $210.00 | in stock | |
| 55120 | 50 mg | $270.00 | 5 days | |
| 65120 | 100 mg |
$420.00
|
5 days | |
| 85120 | 1 g |
please inquire
|
in stock | |
| 95120 | 5 g |
please inquire
|
in stock |
Hexynoic acid STP ester
Alkyne activated ester for the modification of amine groups. STP ester group is an excellent alternative to NHS ester functionality.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 569-25mg | 25 mg | $95.00 | in stock | |
| 569-100mg | 100 mg | $185.00 | in stock | |
| 569-500mg | 500 mg |
$850.00
|
in stock |
Pyrenebutyric acid NHS ester
Pyrene NHS ester for the labeling of biomolecules with pyrene residue. Pyrene is a fluorescent proximity probe which forms excimers, and it is also an anchor group for graphite immobilization.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 63520 | 100 mg | $110.00 | in stock |
ROX NHS ester, 5-isomer
Activated NHS ester of ROX (rhodamine X or rhodamine 101), pure 5-isomer, for the labeling of amine groups of biomolecules.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11220 | 1 mg | $110.00 | in stock | |
| 21220 | 5 mg | $210.00 | in stock | |
| 41220 | 25 mg |
$410.00
|
in stock | |
| 51220 | 50 mg |
$695.00
|
in stock | |
| 61220 | 100 mg |
$1190.00
|
in stock |
ROX NHS ester, 6- isomer
Pure isomer 6-ROX NHS ester for amine labeling.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 12220 | 1 mg | $110.00 | in stock | |
| 22220 | 5 mg | $210.00 | in stock | |
| 42220 | 25 mg |
$410.00
|
in stock | |
| 52220 | 50 mg |
$695.00
|
in stock | |
| 62220 | 100 mg |
$1190.00
|
in stock |
Sulfo-Cyanine3 NHS ester
Water soluble sulfo-Cyanine3 SE activated ester for amino-biomolecule labeling.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11320 | 1 mg | $110.00 | in stock | |
| 21320 | 5 mg | $210.00 | in stock | |
| 41320 | 25 mg |
$410.00
|
in stock | |
| 51320 | 50 mg |
$695.00
|
in stock | |
| 61320 | 100 mg |
$1190.00
|
in stock |
Sulfo-Cyanine3.5 NHS ester
Amine reactive activated ester of water soluble, sulfonated sulfo-Cyanine3.5 dye.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 12320 | 1 mg | $110.00 | in stock | |
| 22320 | 5 mg | $290.00 | in stock | |
| 42320 | 25 mg |
$690.00
|
in stock | |
| 52320 | 50 mg |
$1270.00
|
in stock | |
| 62320 | 100 mg |
$1990.00
|
in stock |
Sulfo-Cyanine5 bis-NHS ester
Water soluble amine reactive Cyanine5 dye with two NHS ester groups.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 133D0 | 1 mg | $110.00 | in stock | |
| 233D0 | 5 mg | $290.00 | in stock | |
| 433D0 | 25 mg |
$690.00
|
in stock | |
| 533D0 | 50 mg |
$1270.00
|
in stock | |
| 633D0 | 100 mg |
$1990.00
|
in stock |
Sulfo-Cyanine5 NHS ester
Water-soluble Cyanine 5 succinimide ester.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 13320 | 1 mg | $110.00 | in stock | |
| 23320 | 5 mg | $210.00 | in stock | |
| 43320 | 25 mg |
$410.00
|
in stock | |
| 53320 | 50 mg |
$695.00
|
in stock | |
| 63320 | 100 mg |
$1190.00
|
in stock |
Sulfo-Cyanine5.5 NHS ester
Water soluble, sulfonated, far-red sulfo-Cyanine5.5 dye in the form of NHS ester.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 17320 | 1 mg | $110.00 | in stock | |
| 27320 | 5 mg | $290.00 | in stock | |
| 47320 | 25 mg |
$690.00
|
in stock | |
| 57320 | 50 mg |
$1190.00
|
in stock | |
| 67320 | 100 mg |
$1850.00
|
in stock |
Sulfo-Cyanine7 bis-NHS ester
Sulfo-Cyanine7 bis-NHS ester is a bifunctional derivative of water soluble near infrared sulfo-Cyanine7. It contains two activated ester functional groups.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 153D0 | 1 mg | $110.00 | in stock | |
| 253D0 | 5 mg | $290.00 | in stock | |
| 453D0 | 25 mg |
$690.00
|
in stock | |
| 553D0 | 50 mg |
$1270.00
|
in stock | |
| 653D0 | 100 mg |
$1990.00
|
in stock |
Sulfo-Cyanine7 NHS ester
Sulfo-Cyanine7 NHS ester is a water soluble, near infrared, amine-reactive dye.
Show pricing
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 15320 | 1 mg | $110.00 | in stock | |
| 25320 | 5 mg | $290.00 | in stock | |
| 45320 | 25 mg |
$690.00
|
in stock |