Primary or secondary amino groups are ubiquitous in biomolecules. They are present in proteins and peptides as ε-amino groups of lysines and N-terminal amino groups. Many natural and synthetic small molecules are amines, and DNAs with amino groups can be prepared by solid phase synthesis, and enzymatically.
Amino groups can be modified by acylation with various activated carbonyl compounds. Among these compounds, activated N-hydroxysuccinimide esters are most suitable for biolabeling and bioconjugation applications.
Compared to other activated esters, NHS derivatives possess good stability in aqueous environment. Optimal pH for the coupling of amines with NHS esters in aqueous buffers is 8.3-8.5. Labeling is also possible in organic solvents. Polar aprotic solents, such as DMF or DMSO, are preferred. One equivalent of tertiary amine base, such as triethylamine, or diisopropylethylamine (DIPEA) is necessary to achieve efficient amine acylation in non-aqueous solution.
Ready-to-use kit for labeling antibodies and other proteins with AF 488 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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Ready-to-use kit for labeling antibodies and other proteins with AF 594 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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Ready to use kit for the labeling of antibodies and other proteins with sulfo-Cyanine3 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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Ready-to-use kit for labeling antibodies and other proteins with sulfo-Cyanine5 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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Ready-to-use kit for labeling antibodies and other proteins with sulfo-Cyanine5.5 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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Ready-to-use kit for labeling antibodies and other proteins with sulfo-Cyanine7 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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Ready-to-use kit for labeling antibodies and other proteins with sulfo-Cyanine7.5 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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Amine-reactive fluorescein (FAM) derivative, a mixture of 5- and 6-isomers. The reagent becomes brightly green fluorescent once it is hydrolyzed while diffusing into cells. A fluorescent tracer.
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AF 343 (Coumarin) is a blue emitting dye that can serve as a FRET donor for fluorescein (FAM). This structure features a linker between Coumarin 343 fluorophore, and the functional group. It provides better solubility, and spatial separation between the dye, and the functional group.
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AF 430 is a hydrophilic coumarin dye with emission in the green-yellow range. The NHS ester is used for the labeling of amine groups.
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AF 488 NHS ester, a bright, photostable, and hydrophilic fluorescent dye for the green channel.
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NHS-ester of AF 532 dye for protein, peptide, and modified oligonucleotide labeling via reaction with amino groups.
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1C820
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AF 555 NHS ester, a bright, photostable, and hydrophilic fluorescent dye for the yellow channel.
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AF 568 derivative for labeling proteins and other high- and low-molecular weight ligands with free amines.
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Red-fluorescent NHS-ester of AF 594 dye for labeling proteins, peptides, antibodies, and any molecules containing an -NH2 group. Pure 5-isomer
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Bright, water-soluble succinimide ester of AF 647 dye for protein and antibody labeling.
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Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on diethyleneglycol (PEG2).
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Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on triethyleneglycol (PEG3).
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Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on tetraethyleneglycol (PEG4).
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2409-100mg
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NHS-ester of AMCA dye for protein, peptide, and modified oligonucleotide labeling via reaction with amino groups. AMCA is one of the brightest blue fluorescent dyes with large Stoke's shift and high resistance to photobleaching.
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3606-5mg
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$110.00
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A hydrophilic bifunctional linker with azide and NHS ester function groups flanking PEG6 (hexaethylene glycol).
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250 mg
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Activated NHS ester for azido-labeling of peptides and proteins.
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73720
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BDP 558/568 is a fluorophore for the yellow region of the spectrum. Like other BDP dyes, this fluorophore has high fluorescence quantum yield, brightness, and photostability.
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47420
25 mg
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50 mg
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Lypohilic borodipyrromethene dye with orange emission for the labeling of primary and secondary amine groups.
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BDP 576/589 NHS ester is a bright hydrophobic dye with emission in the orange spectrum range. It can be used in fluorescence anisotropy assays.
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NHS ester derivative of BDP 581/591 borondipyrromethene fluorophore for the labeling of proteins, peptides, and other biomolecules. The dye is useful for fluorescence polarization and two-photon experiments, and as a ROS probe.
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BDP 630/650 is a borondipyrromethene fluorophore tuned to Cyanine5 excitation and emission channels. The dye is bright and highly photostable. It is useful for fluorescence polarization assays. This is an amine-reactive NHS ester derivative.
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Amine reactive of BDP 650/665, a borondipyrromethene dye for Cy5 channel, possessing a high molar extinction coefficient, and a good emission quantum yield.
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Amino-reactive BDP FL, a very bright and photostable dye for fluorescein channel.
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BDP R6G is a borondipyrromethene dye which has absorption and emission spectra similar to R6G.
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Amine reactive NHS ester of BDP TMR, bright borondipyrromethene dye for TAMRA channel especially suitable for fluorescence polarization assays.
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Amine reactive NHS ester of BDP TMR, bright borondipyrromethene dye for TAMRA channel especially suitable for fluorescence polarization assays. This compound contains an aminohexanoic acid (C6) linker between the dye and the functional group.
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BDP TR is a borondipyrromethene dye. A bright and photostable fluorophore with high quantum yield.
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BDP TR X NHS ester is a derivative of BDP TR containing a long linker based on aminohexanoic acid (C6). The dye has absorption and emission close to ROX. The NHS ester function can be used to conjugate proteins and peptides.
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Biotin derivative with C6-spacer to reduce steric effect when binding amino acids, peptides, or proteins by reacting with primary amines.
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2551-100mg
100 mg
$117.00
in stock
2551-1g
1 g
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United States , ZIP
Items in stock will be shipped on
12/11/2023
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PEG4-containing activated ester for simple and efficient biotin labeling of antibodies, proteins, and other primary amine-containing biomolecules.
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2893-100mg
100 mg
$145.00
in stock
2893-1g
1 g
$590.00
in stock
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United States , ZIP
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Cyanine2 NHS ester minimal dye for protein labeling
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1A041
5 nmol
$110.00
in stock
2A041
10 nmol
$140.00
in stock
3A041
25 nmol
$265.00
in stock
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United States , ZIP
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Amine-reactive Cyanine3 dye NHS ester.
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11020
1 mg
$125.00
in stock
21020
5 mg
$260.00
in stock
41020
25 mg
$510.00
in stock
51020
50 mg
$895.00
in stock
61020
100 mg
$1490.00
in stock
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United States , ZIP
Items in stock will be shipped on
12/11/2023
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$400 !
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to your cart and get free express shipping to
United States , ZIP
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$400 !
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Cyanine3 minimal dye for protein labeling.
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1B041
5 nmol
$110.00
in stock
2B041
10 nmol
$175.00
in stock
3B041
25 nmol
$265.00
in stock