Primary or secondary amino groups are ubiquitous in biomolecules. They are present in proteins and peptides as ε-amino groups of lysines and N-terminal amino groups. Many natural and synthetic small molecules are amines, and DNAs with amino groups can be prepared by solid phase synthesis, and enzymatically.
Amino groups can be modified by acylation with various activated carbonyl compounds. Among these compounds, activated N-hydroxysuccinimide esters are most suitable for biolabeling and bioconjugation applications.
Compared to other activated esters, NHS derivatives possess good stability in aqueous environment. Optimal pH for the coupling of amines with NHS esters in aqueous buffers is 8.3-8.5. Labeling is also possible in organic solvents. Polar aprotic solents, such as DMF or DMSO, are preferred. One equivalent of tertiary amine base, such as triethylamine, or diisopropylethylamine (DIPEA) is necessary to achieve efficient amine acylation in non-aqueous solution.
AF430 is a hydrophilic coumarin dye with the emission in green-yellow range. The NHS ester is used for the labeling of amine groups.
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AF488 (Alexa Fluor 488) NHS ester, a bright, photostable and hydrophilic fluorescent dye for green channel.
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AF568 derivative for labeling proteins and other high- and low-molecular weight ligands with free amines
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Alkyne NHS ester for the labeling of biomolecules with alkyne group for Copper catalyzed Click chemistry.
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Bifunctional hyrophilic linker with NHS ester and terminal alkyne functional group based on triethyleneglycol (PEG3).
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Activated ester for azido labeling of peptides and proteins.
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BDP 558/568 is a fluorophore for the yellow region of the spectrum. Like other BDP dyes, this fluorophore has high fluorescence quantum yield, brightness, and photostability.
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NHS ester derivative of BDP 581/591 borondipyrromethene fluorophore for the labeling of proteins, peptides, and other biomolecules. The dye is useful for fluorescence polarization and two-photon experiments, and as a ROS probe.
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BDP 630/650 is a borondipyrromethene fluorophore tuned to Cyanine5 excitation and emission channels. The dye is bright and highly photostable. It is useful for fluorescence polarization assays This is an amine-reactive NHS ester derivative.
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Amine reactive of BDP 650/665, a borondipyrromethene dye for Cy5 channel, possessing a high molar extinction coefficient, and a good emission quantum yield.
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Amino-reactive BDP FL, a very bright and photostable dye for fluorescein channel.
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BDP R6G is a borondipyrromethene dye which has absorption and emission spectra similar to R6G.
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Amine reactive NHS ester of BDP TMR, bright borondipyrromethene dye for TAMRA channel especially suitable for fluorescence polarization assays.
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BDP TR is a borondipyrromethene dye for Texas Red channel. A bright and photostable fluorophore with high quantum yield.
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BDP TR X NHS ester is a derivative of BDP TR containing a long linker based on aminohexanoic acid (C6). The dye has absorption and emission close to ROX (Texas Red). The NHS ester function can be used for the conjugation with proteins and peptides.
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Coumarin 343 is a blue emitting dye that can serve as a FRET donor for fluorescein (FAM). This structure features a linker between Coumarin 343 fluorophore, and the functional group. It provides better solubility, and spatial separation between the dye, and the functional group.
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Cyanine2 NHS ester minimal dye for protein labeling
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1A041
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Amine-reactive Cyanine3 dye NHS ester.
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Cyanine3 minimal dye for protein labeling.
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1B041
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Cyanine3.5 dye NHS ester derivative.
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Amine-reactive Cyanine5 activated ester for the labeling of proteins, peptides, and other molecules.
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Cy5 NHS ester minimal dye for protein labeling.
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1C041
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Cyanine5.5 NHS is far-red / near-infrared amine-reactive dye.
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Amine reactive NHS ester of near infrared dye Cyanine7.
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Amine reactive NHS ester of NIR fluorescent dye Cyanine7.5.
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Azodibenzocyclooctyne (DBCO, ADIBO) reagent for strain promoted copper free Click chemistry (spAAC). The reagent contains an NHS ester function for the attachment of the cyclooctyne to various molecules.
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Activated N-hydroxysuccinimide ester of fluorescein (FAM), pure 6-isomer.
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Alkyne activated ester for the modification of amine groups. STP ester group is an excellent alternative to NHS ester functionality.
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569-25mg
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Pyrene NHS ester for the labeling of biomolecules with pyrene residue. Pyrene is a fluorescent proximity probe which forms excimers, and it is also an anchor group for graphite immobilization.
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Activated NHS ester of ROX (rhodamine X or rhodamine 101), pure 5-isomer, for the labeling of amine groups of biomolecules.
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Pure isomer 6-ROX NHS ester for amine labeling.
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Water soluble sulfo-Cyanine3 SE activated ester for amino-biomolecule labeling.
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Amine reactive activated ester of water soluble, sulfonated sulfo-Cyanine3.5 dye.
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Water soluble amine reactive Cyanine5 dye with two NHS ester groups.
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133D0
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Water-soluble Cyanine 5 succinimide ester.
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Water soluble, sulfonated, far-red sulfo-Cyanine5.5 dye in the form of NHS ester.
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17320
1 mg
$110.00
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27320
5 mg
$290.00
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47320
25 mg
$690.00
in stock
57320
50 mg
$1190.00
in stock
67320
100 mg
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Sulfo-Cyanine7 bis-NHS ester is a bifunctional derivative of water soluble near infrared sulfo-Cyanine7. It contains two activated ester functional groups.
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153D0
1 mg
$110.00
in stock
253D0
5 mg
$290.00
in stock
453D0
25 mg
$690.00
in stock
553D0
50 mg
$1270.00
in stock
653D0
100 mg
$1990.00
in stock
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Sulfo-Cyanine7 NHS ester is a water soluble, near infrared, amine-reactive dye.
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15320
1 mg
$110.00
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25320
5 mg
$290.00
in stock
45320
25 mg
$690.00
in stock
55320
50 mg
$1270.00
in stock
65320
100 mg
$1990.00
in stock
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A water soluble near infrared emitting dye for in vivo imaging applications. Sulfo-Cyanine7.5 is an analog of indocyanine green (ICG) possessing higher emission quantum yield, and containing a linker arm.
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16320
1 mg
$110.00
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26320
5 mg
$290.00
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46320
25 mg
$690.00
in stock
56320
50 mg
$1190.00
in stock
66320
100 mg
$1850.00
in stock
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03/08/2021
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Amine-reactive, activated N-hydroxysuccinimidyl ester of TAMRA dye for the labeling of amine groups of proteins, peptides, and amino-oligonucleotides.
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17120
1 mg
$110.00
in stock
27120
5 mg
$210.00
in stock
47120
25 mg
$410.00
in stock
57120
50 mg
$695.00
in stock
67120
100 mg
$1190.00
in stock
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