NHS esters in Reactive groups
Primary or secondary amino groups are ubiquitous in biomolecules. They are present in proteins and peptides as ε-amino groups of lysines and N-terminal amino groups. Many natural and synthetic small molecules are amines, and DNAs with amino groups can be prepared by solid phase synthesis, and enzymatically.
Amino groups can be modified by acylation with various activated carbonyl compounds. Among these compounds, activated N-hydroxysuccinimide esters are most suitable for biolabeling and bioconjugation applications.
Compared to other activated esters, NHS derivatives possess good stability in aqueous environment. Optimal pH for the coupling of amines with NHS esters in aqueous buffers is 8.3-8.5. Labeling is also possible in organic solvents. Polar aprotic solents, such as DMF or DMSO, are preferred. One equivalent of tertiary amine base, such as triethylamine, or diisopropylethylamine (DIPEA) is necessary to achieve efficient amine acylation in non-aqueous solution.
AF 488 antibody labeling kit
Ready-to-use kit for labeling antibodies and other proteins with AF 488 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1821-1rxn |
1 rxn
|
$69 | 21 days | |
| 1821-10rxn |
10 rxn
|
$450
|
21 days |
AF 594 antibody labeling kit
Ready-to-use kit for labeling antibodies and other proteins with AF 594 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 6821-1rxn |
1 rxn
|
$69 | 21 days | |
| 6821-10rxn |
10 rxn
|
$450
|
21 days |
sulfo-Cyanine3 antibody labeling kit
Ready to use kit for the labeling of antibodies and other proteins with sulfo-Cyanine3 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1321-1rxn |
1 rxn
|
$69 | 21 days | |
| 1321-10rxn |
10 rxn
|
$450
|
21 days |
sulfo-Cyanine5 antibody labeling kit
Ready-to-use kit for labeling antibodies and other proteins with sulfo-Cyanine5 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3321-1rxn |
1 rxn
|
$69 | 21 days | |
| 3321-10rxn |
10 rxn
|
$397 | 21 days |
sulfo-Cyanine5.5 antibody labeling kit
Ready-to-use kit for labeling antibodies and other proteins with sulfo-Cyanine5.5 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 7321-1rxn |
1 rxn
|
$69 | 21 days | |
| 7321-10rxn |
10 rxn
|
$450
|
21 days |
sulfo-Cyanine7 antibody labeling kit
Ready-to-use kit for labeling antibodies and other proteins with sulfo-Cyanine7 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 5321-1rxn |
1 rxn
|
$69 | 21 days | |
| 5321-10rxn |
10 rxn
|
$450
|
21 days |
sulfo-Cyanine7.5 antibody labeling kit
Ready-to-use kit for labeling antibodies and other proteins with sulfo-Cyanine7.5 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 6321-1rxn |
1 rxn
|
$69 | 21 days | |
| 6321-10rxn |
10 rxn
|
$450
|
21 days |
(5,6)-FAM diacetate NHS ester
Amine-reactive fluorescein (FAM) derivative, a mixture of 5- and 6-isomers. The reagent becomes brightly green fluorescent once it is hydrolyzed while diffusing into cells. A fluorescent tracer.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2934-25mg | 25 mg | $160 | in stock | |
| 2934-50mg | 50 mg | $270 | in stock | |
| 2934-100mg | 100 mg |
$520
|
in stock |
3-Maleimidopropionic acid NHS-ester
Bifunctional crosslinker with an amine-reactive succinimidyl ester and a thiol-reactive maleimide group.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3419-500mg | 500 mg | $110 | in stock |
AF 343 (Coumarin)-X-NHS ester
AF 343 (Coumarin) is a blue emitting dye that can serve as a FRET donor for fluorescein (FAM). This structure features a linker between Coumarin 343 fluorophore, and the functional group. It provides better solubility, and spatial separation between the dye, and the functional group.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 12620 | 1 mg | $110 | in stock | |
| 22620 | 5 mg | $210 | in stock | |
| 42620 | 25 mg |
$410
|
in stock | |
| 52620 | 50 mg |
$695
|
in stock | |
| 62620 | 100 mg |
$1190
|
in stock |
AF 430 NHS ester
AF 430 is a hydrophilic coumarin dye with emission in the green-yellow range. The NHS ester is used for the labeling of amine groups.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 10820 | 1 mg | $110 | in stock | |
| 20820 | 5 mg | $165 | in stock | |
| 40820 | 25 mg | $380 | in stock | |
| 50820 | 50 mg |
$695
|
in stock | |
| 60820 | 100 mg |
$1190
|
in stock |
AF 488 NHS ester
AF 488 NHS ester, a bright, photostable, and hydrophilic fluorescent dye for the green channel.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11820 | 1 mg | $125 | in stock | |
| 21820 | 5 mg | $325 | in stock | |
| 31820 | 10 mg |
$480
|
in stock | |
| 41820 | 25 mg |
$950
|
in stock | |
| 51820 | 50 mg |
$1625
|
in stock | |
| 61820 | 100 mg |
$2090
|
in stock |
AF 532 NHS ester
NHS-ester of AF 532 dye for protein, peptide, and modified oligonucleotide labeling via reaction with amino groups.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1C820 | 1 mg | $140 | in stock | |
| 2C820 | 5 mg |
$450
|
in stock | |
| 4C820 | 25 mg |
$1460
|
in stock | |
| 5C820 | 50 mg |
$1890
|
in stock | |
| 6C820 | 100 mg |
$2640
|
in stock |
AF 555 NHS ester
AF 555 NHS ester, a bright, photostable, and hydrophilic fluorescent dye for the yellow channel.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1K820 | 1 mg | $140 | in stock | |
| 2K820 | 5 mg |
$450
|
in stock | |
| 4K820 | 25 mg |
$1460
|
in stock | |
| 5K820 | 50 mg |
$1890
|
in stock | |
| 6K820 | 100 mg |
$2640
|
in stock |
AF 568 NHS ester
AF 568 derivative for labeling proteins and other high- and low-molecular weight ligands with free amines.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 14820 | 1 mg | $125 | in stock | |
| 24820 | 5 mg | $325 | in stock | |
| 44820 | 25 mg |
$720
|
in stock | |
| 54820 | 50 mg |
$1325
|
in stock | |
| 64820 | 100 mg |
$2090
|
in stock |
AF 594 NHS ester
Red-fluorescent NHS-ester of AF 594 dye for labeling proteins, peptides, antibodies, and any molecules containing an -NH2 group. Pure 5-isomer
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2825-1mg | 1 mg | $125 | in stock | |
| 2825-5mg | 5 mg |
$425
|
in stock | |
| 2825-25mg | 25 mg |
$1240
|
in stock | |
| 2825-50mg | 50 mg |
$1890
|
in stock | |
| 2825-100mg | 100 mg |
$2640
|
in stock |
AF 647 NHS ester
Bright, water-soluble succinimide ester of AF 647 dye for protein and antibody labeling.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 16820 | 1 mg | $140 | in stock | |
| 26820 | 5 mg |
$450
|
in stock | |
| 46820 | 25 mg |
$1460
|
in stock | |
| 56820 | 50 mg |
$1890
|
in stock | |
| 66820 | 100 mg |
$2640
|
in stock |
Alkyne-PEG2-NHS ester
Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on diethyleneglycol (PEG2).
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2219-1g | 1 g |
$450
|
in stock | |
| 2219-5g | 5 g |
$980
|
in stock |
Alkyne-PEG3-NHS ester
Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on triethyleneglycol (PEG3).
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2298-1g | 1 g |
$490
|
in stock | |
| 2298-5g | 5 g |
$980
|
in stock |