NHS esters in Reactive groups

Primary or secondary amino groups are ubiquitous in biomolecules. They are present in proteins and peptides as ε-amino groups of lysines and N-terminal amino groups. Many natural and synthetic small molecules are amines, and DNAs with amino groups can be prepared by solid phase synthesis, and enzymatically.

Amino groups can be modified by acylation with various activated carbonyl compounds. Among these compounds, activated N-hydroxysuccinimide esters are most suitable for biolabeling and bioconjugation applications.

NHS ester coupling chemistry

Compared to other activated esters, NHS derivatives possess good stability in aqueous environment. Optimal pH for the coupling of amines with NHS esters in aqueous buffers is 8.3-8.5. Labeling is also possible in organic solvents. Polar aprotic solents, such as DMF or DMSO, are preferred. One equivalent of tertiary amine base, such as triethylamine, or diisopropylethylamine (DIPEA) is necessary to achieve efficient amine acylation in non-aqueous solution.

AF 488 antibody labeling kit

Ready-to-use kit for labeling antibodies and other proteins with AF 488 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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Cat. # Quantity Price Lead time
1821-1rxn
1 rxn
$69 1 days
1821-10rxn
10 rxn
$450 1 days
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AF 594 antibody labeling kit

Ready-to-use kit for labeling antibodies and other proteins with AF 594 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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Cat. # Quantity Price Lead time
6821-1rxn
1 rxn
$69 1 days
6821-10rxn
10 rxn
$450 21 days
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sulfo-Cyanine3 antibody labeling kit

Ready to use kit for the labeling of antibodies and other proteins with sulfo-Cyanine3 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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Cat. # Quantity Price Lead time
1321-1rxn
1 rxn
$69 1 days
1321-10rxn
10 rxn
$450 1 days
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sulfo-Cyanine5 antibody labeling kit

Ready-to-use kit for labeling antibodies and other proteins with sulfo-Cyanine5 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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Cat. # Quantity Price Lead time
3321-1rxn
1 rxn
$69 1 days
3321-10rxn
10 rxn
$397 1 days
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sulfo-Cyanine5.5 antibody labeling kit

Ready-to-use kit for labeling antibodies and other proteins with sulfo-Cyanine5.5 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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Cat. # Quantity Price Lead time
7321-1rxn
1 rxn
$69 1 days
7321-10rxn
10 rxn
$450 1 days
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sulfo-Cyanine7 antibody labeling kit

Ready-to-use kit for labeling antibodies and other proteins with sulfo-Cyanine7 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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Cat. # Quantity Price Lead time
5321-1rxn
1 rxn
$69 1 days
5321-10rxn
10 rxn
$450 1 days
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sulfo-Cyanine7.5 antibody labeling kit

Ready-to-use kit for labeling antibodies and other proteins with sulfo-Cyanine7.5 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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Cat. # Quantity Price Lead time
6321-1rxn
1 rxn
$69 1 days
6321-10rxn
10 rxn
$450 1 days
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(5,6)-FAM diacetate NHS ester

Amine-reactive fluorescein (FAM) derivative, a mixture of 5- and 6-isomers. The reagent becomes brightly green fluorescent once it is hydrolyzed while diffusing into cells. A fluorescent tracer.
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Cat. # Quantity Price Lead time
2934-25mg 25 mg $160 in stock
2934-50mg 50 mg $270 in stock
2934-100mg 100 mg $520 in stock
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3-Maleimidopropionic acid NHS-ester

Bifunctional crosslinker with an amine-reactive succinimidyl ester and a thiol-reactive maleimide group.
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Cat. # Quantity Price Lead time
3419-500mg 500 mg $110 in stock
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AF 343 (Coumarin)-X-NHS ester

AF 343 (Coumarin) is a blue emitting dye that can serve as a FRET donor for fluorescein (FAM). This structure features a linker between Coumarin 343 fluorophore, and the functional group. It provides better solubility, and spatial separation between the dye, and the functional group.
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Cat. # Quantity Price Lead time
12620 1 mg $110 in stock
22620 5 mg $210 in stock
42620 25 mg $410 in stock
52620 50 mg $695 in stock
62620 100 mg $1190 in stock
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AF 430 NHS ester

AF 430 is a hydrophilic coumarin dye with emission in the green-yellow range. The NHS ester is used for the labeling of amine groups.
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Cat. # Quantity Price Lead time
10820 1 mg $110 in stock
20820 5 mg $165 in stock
40820 25 mg $380 in stock
50820 50 mg $695 in stock
60820 100 mg $1190 in stock
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AF 488 NHS ester

AF 488 NHS ester, a bright, photostable, and hydrophilic fluorescent dye for the green channel.
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Cat. # Quantity Price Lead time
11820 1 mg $125 in stock
21820 5 mg $325 in stock
31820 10 mg $480 in stock
41820 25 mg $950 in stock
51820 50 mg $1625 in stock
61820 100 mg $2090 in stock
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AF 532 NHS ester

NHS-ester of AF 532 dye for protein, peptide, and modified oligonucleotide labeling via reaction with amino groups.
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Cat. # Quantity Price Lead time
1C820 1 mg $140 in stock
2C820 5 mg $450 in stock
4C820 25 mg $1460 in stock
5C820 50 mg $1890 in stock
6C820 100 mg $2640 5 days
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AF 555 NHS ester

AF 555 NHS ester, a bright, photostable, and hydrophilic fluorescent dye for the yellow channel.
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Cat. # Quantity Price Lead time
1K820 1 mg $140 in stock
2K820 5 mg $450 in stock
4K820 25 mg $1460 in stock
5K820 50 mg $1890 in stock
6K820 100 mg $2640 in stock
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AF 568 NHS ester

AF 568 derivative for labeling proteins and other high- and low-molecular weight ligands with free amines.
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Cat. # Quantity Price Lead time
14820 1 mg $125 in stock
24820 5 mg $325 in stock
44820 25 mg $720 in stock
54820 50 mg $1325 in stock
64820 100 mg $2090 in stock
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AF 594 NHS ester

Red-fluorescent NHS-ester of AF 594 dye for labeling proteins, peptides, antibodies, and any molecules containing an -NH2 group. Pure 5-isomer
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Cat. # Quantity Price Lead time
2825-1mg 1 mg $125 5 days
2825-5mg 5 mg $425 5 days
2825-25mg 25 mg $1240 in stock
2825-50mg 50 mg $1890 in stock
2825-100mg 100 mg $2640 5 days
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AF 647 NHS ester

Bright, water-soluble succinimide ester of AF 647 dye for protein and antibody labeling.
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Cat. # Quantity Price Lead time
16820 1 mg $140 in stock
26820 5 mg $450 in stock
46820 25 mg $1460 in stock
56820 50 mg $1890 in stock
66820 100 mg $2640 in stock
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Alkyne-PEG2-NHS ester

Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on diethyleneglycol (PEG2).
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Cat. # Quantity Price Lead time
2219-1g 1 g $450 in stock
2219-5g 5 g $980 in stock
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Alkyne-PEG3-NHS ester

Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on triethyleneglycol (PEG3).
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Cat. # Quantity Price Lead time
2298-1g 1 g $490 in stock
2298-5g 5 g $980 in stock
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Alkyne-PEG4-NHS ester

Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on tetraethyleneglycol (PEG4).
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Cat. # Quantity Price Lead time
2409-100mg 100 mg $250 in stock
2409-1g 1 g $790 in stock
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AMCA NHS ester

NHS-ester of AMCA dye for protein, peptide, and modified oligonucleotide labeling via reaction with amino groups. AMCA is one of the brightest blue fluorescent dyes with large Stoke's shift and high resistance to photobleaching.
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Cat. # Quantity Price Lead time
3606-5mg 5 mg $110 in stock
3606-25mg 25 mg $290 in stock
3606-50mg 50 mg $435 in stock
3606-100mg 100 mg $595 in stock
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ATFB SE (N-Succinimidyl 4-Azidotetrafluorobenzoate)

N-Succinimidyl 4-Azidotetrafluorobenzoate (ATFB SE, ATFB-OSu) is a photo-activatable linker used as a versatile labeling agent for investigating biological receptors.
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Cat. # Quantity Price Lead time
2574-250mg 250 mg $146 12 days
2574-1g 1 g $420 21 days
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Azide-PEG6-NHS ester

A hydrophilic bifunctional linker with azide and NHS ester function groups flanking PEG6 (hexaethylene glycol).
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Cat. # Quantity Price Lead time
3692-250mg 250 mg $325 in stock
3692-1g 1 g $539 in stock
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Azidobutyric acid NHS ester

Activated NHS ester for azido-labeling of peptides and proteins.
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Cat. # Quantity Price Lead time
53720 50 mg $110 in stock
63720 100 mg $210 in stock
73720 1 g $950 in stock
83720 5 g please inquire in stock
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BDP 558/568 NHS ester

BDP 558/568 is a fluorophore for the yellow region of the spectrum. Like other BDP dyes, this fluorophore has high fluorescence quantum yield, brightness, and photostability.
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Cat. # Quantity Price Lead time
17420 1 mg $125 in stock
27420 5 mg $260 in stock
47420 25 mg $510 in stock
57420 50 mg $895 in stock
67420 100 mg $1490 in stock
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BDP 564/570 NHS ester

Lypohilic borodipyrromethene dye with orange emission for the labeling of primary and secondary amine groups.
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Cat. # Quantity Price Lead time
1H420 1 mg $125 in stock
2H420 5 mg $325 in stock
4H420 25 mg $510 in stock
5H420 50 mg $895 in stock
6H420 100 mg $1490 in stock
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BDP 576/589 NHS ester

BDP 576/589 NHS ester is a bright hydrophobic dye with emission in the orange spectrum range. It can be used in fluorescence anisotropy assays.
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Cat. # Quantity Price Lead time
1G420 1 mg $125 in stock
2G420 5 mg $260 in stock
4G420 25 mg $510 in stock
5G420 50 mg $895 in stock
6G420 100 mg $1490 in stock
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BDP 581/591 NHS ester

NHS ester derivative of BDP 581/591 borondipyrromethene fluorophore for the labeling of proteins, peptides, and other biomolecules. The dye is useful for fluorescence polarization and two-photon experiments, and as a ROS probe.
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Cat. # Quantity Price Lead time
16420 1 mg $125 in stock
26420 5 mg $260 in stock
46420 25 mg $510 in stock
56420 50 mg $895 in stock
66420 100 mg $1490 in stock
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BDP 630/650-X-NHS ester

BDP 630/650 is a borondipyrromethene fluorophore tuned to Cyanine5 excitation and emission channels. The dye is bright and highly photostable. It is useful for fluorescence polarization assays. This is an amine-reactive NHS ester derivative.
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Cat. # Quantity Price Lead time
15420 1 mg $125 in stock
25420 5 mg $260 in stock
45420 25 mg $510 in stock
55420 50 mg $895 in stock
65420 100 mg $1490 in stock
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BDP 650/665-X-NHS ester

Amine reactive of BDP 650/665, a borondipyrromethene dye for Cy5 channel, possessing a high molar extinction coefficient, and a good emission quantum yield.
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Cat. # Quantity Price Lead time
1E420 1 mg $125 in stock
2E420 5 mg $260 in stock
4E420 25 mg $510 in stock
5E420 50 mg $895 in stock
6E420 100 mg $1490 in stock
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BDP FL NHS ester

Amino-reactive BDP FL, a very bright and photostable dye for fluorescein channel.
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Cat. # Quantity Price Lead time
11420 1 mg $125 $98 in stock
21420 5 mg $260 $175 5 days
41420 25 mg $485 in stock
51420 50 mg $824 in stock
61420 100 mg $1380 in stock
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BDP R6G NHS ester

BDP R6G is a borondipyrromethene dye which has absorption and emission spectra similar to R6G.
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Cat. # Quantity Price Lead time
14420 1 mg $125 in stock
24420 5 mg $260 in stock
44420 25 mg $510 in stock
54420 50 mg $895 in stock
64420 100 mg $1490 in stock
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BDP TMR NHS ester

Amine reactive NHS ester of BDP TMR, bright borondipyrromethene dye for TAMRA channel especially suitable for fluorescence polarization assays.
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Cat. # Quantity Price Lead time
12420 1 mg $125 in stock
22420 5 mg $260 in stock
42420 25 mg $510 in stock
52420 50 mg $895 in stock
62420 100 mg $1490 in stock