NHS esters in Reactive groups

Primary or secondary amino groups are ubiquitous in biomolecules. They are present in proteins and peptides as ε-amino groups of lysines and N-terminal amino groups. Many natural and synthetic small molecules are amines, and DNAs with amino groups can be prepared by solid phase synthesis, and enzymatically.

Amino groups can be modified by acylation with various activated carbonyl compounds. Among these compounds, activated N-hydroxysuccinimide esters are most suitable for biolabeling and bioconjugation applications.

NHS ester coupling chemistry

Compared to other activated esters, NHS derivatives possess good stability in aqueous environment. Optimal pH for the coupling of amines with NHS esters in aqueous buffers is 8.3-8.5. Labeling is also possible in organic solvents. Polar aprotic solents, such as DMF or DMSO, are preferred. One equivalent of tertiary amine base, such as triethylamine, or diisopropylethylamine (DIPEA) is necessary to achieve efficient amine acylation in non-aqueous solution.

AF 488 antibody labeling kit

Ready-to-use kit for labeling antibodies and other proteins with AF 488 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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Cat. # Quantity Price Lead time
1821-10rxn
10 rxn
$450.00 in stock

AF 594 antibody labeling kit

Ready-to-use kit for labeling antibodies and other proteins with AF 594 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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Cat. # Quantity Price Lead time
6821-10rxn
10 rxn
$450.00 in stock

sulfo-Cyanine3 antibody labeling kit

Ready to use kit for the labeling of antibodies (other proteins can be labeled as well) with sulfo-Cyanine3 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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Cat. # Quantity Price Lead time
1321-10rxn
10 rxn
$450.00 in stock

sulfo-Cyanine5 antibody labeling kit

A kit for the labeling of antibodies with sulfo-Cyanine5 dye, a fluorophore for Cy5 channel.
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Cat. # Quantity Price Lead time
3321-10rxn
10 rxn
$400.00 in stock

sulfo-Cyanine5.5 antibody labeling kit

A kit for the labeling of antibodies with sulfo-Cyanine5.5 dye, a fluorophore for Cy5.5 channel.
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Cat. # Quantity Price Lead time
7321-10rxn
10 rxn
$450.00 in stock

sulfo-Cyanine7 antibody labeling kit

A kit for the labeling of antibodies with sulfo-Cyanine7 dye, a fluorophore for Cy7 channel.
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Cat. # Quantity Price Lead time
5321-10rxn
10 rxn
$450.00 in stock

sulfo-Cyanine7.5 antibody labeling kit

A kit for the labeling of antibodies with sulfo-Cyanine7.5 dye, a fluorophore for Cy7.5 channel.
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Cat. # Quantity Price Lead time
6321-10rxn
10 rxn
$450.00 in stock

(5,6)-FAM diacetate NHS ester

Amine-reactive fluorescein (FAM) derivative, a mixture of 5- and 6-isomers. The reagent becomes brightly green fluorescent once it is hydrolyzed while diffusing into cells. A fluorescent tracer.
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Cat. # Quantity Price Lead time
2934-25mg 25 mg $160.00 in stock
2934-50mg 50 mg $270.00 in stock
2934-100mg 100 mg $520.00 in stock

AF 430 NHS ester

AF 430 is a hydrophilic coumarin dye with emission in the green-yellow range. The NHS ester is used for the labeling of amine groups.
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Cat. # Quantity Price Lead time
10820 1 mg $110.00 in stock
20820 5 mg $165.00 in stock
40820 25 mg $380.00 in stock
50820 50 mg $695.00 in stock
60820 100 mg $1190.00 in stock

AF 488 NHS ester

AF 488 NHS ester, a bright, photostable, and hydrophilic fluorescent dye for the green channel.
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Cat. # Quantity Price Lead time
11820 1 mg $110.00 in stock
21820 5 mg $290.00 in stock
31820 10 mg $470.00 in stock
41820 25 mg $690.00 in stock
51820 50 mg $1270.00 in stock
61820 100 mg $1990.00 in stock

AF 555 NHS ester

AF 555 NHS ester, a bright, photostable, and hydrophilic fluorescent dye for the yellow channel.
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Cat. # Quantity Price Lead time
1K820 1 mg $120.00 in stock
2K820 5 mg $400.00 in stock
4K820 25 mg $1210.00 in stock
5K820 50 mg $1710.00 12 days
6K820 100 mg $2425.00 in stock

AF 568 NHS ester

AF 568 derivative for labeling proteins and other high- and low-molecular weight ligands with free amines.
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Cat. # Quantity Price Lead time
14820 1 mg $110.00 5 days
24820 5 mg $290.00 5 days
44820 25 mg $690.00 in stock
54820 50 mg $1270.00 in stock
64820 100 mg $1990.00 in stock

AF 594 NHS ester, 5-isomer

Red-fluorescent NHS-ester of AF 594 dye for labeling proteins, peptides, antibodies, and any molecules containing an -NH2 group
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Cat. # Quantity Price Lead time
2825-1mg 1 mg $120.00 in stock
2825-5mg 5 mg $355.00 in stock
2825-25mg 25 mg $1150.00 in stock
2825-50mg 50 mg $1955.00 in stock
2825-100mg 100 mg $2425.00 in stock

AF 647 NHS ester

Bright, water-soluble succinimide ester of AF 647 dye for protein and antibody labeling.
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Cat. # Quantity Price Lead time
16820 1 mg $110.00 in stock
26820 5 mg $340.00 in stock
46820 25 mg $1100.00 in stock
56820 50 mg $1890.00 in stock
66820 100 mg $2990.00 in stock

Alkyne-PEG2-NHS ester

Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on diethyleneglycol (PEG2).
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Cat. # Quantity Price Lead time
2219-1g 1 g $490.00 in stock
2219-5g 5 g $980.00 21 days

Alkyne-PEG3-NHS ester

Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on triethyleneglycol (PEG3).
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Cat. # Quantity Price Lead time
2298-1g 1 g $490.00 in stock
2298-5g 5 g $980.00 21 days
2298-25g 25 g please inquire 21 days

Alkyne-PEG4-NHS ester

Bifunctional hydrophilic linker with NHS ester and terminal alkyne functional group based on tetraethyleneglycol (PEG4).
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Cat. # Quantity Price Lead time
2409-1g 1 g $790.00 21 days

AMCA NHS ester

NHS-ester of AMCA dye for proteins, peptides, and modified oligonucleotides labeling via reaction with amino groups. AMCA is one of the brightest blue fluorescent dyes with large Stoke's shift and high resistance to photobleaching.
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Cat. # Quantity Price Lead time
3606-5mg 5 mg $110.00 in stock
3606-25mg 25 mg $290.00 in stock
3606-50mg 50 mg $435.00 in stock
3606-100mg 100 mg $595.00 in stock

Azide-PEG6-NHS ester

A hydrophilic bifunctional linker with azide and NHS ester function groups flanking PEG6 (hexaethylene glycol).
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Cat. # Quantity Price Lead time
3692-250mg 250 mg $325.00 12 days
3692-1g 1 g $539.00 in stock

Azidobutyric acid NHS ester

Activated NHS ester for azido-labeling of peptides and proteins.
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Cat. # Quantity Price Lead time
53720 50 mg $60.00 in stock
63720 100 mg $95.00 in stock
73720 1 g please inquire in stock
83720 5 g please inquire in stock

BDP 558/568 NHS ester

BDP 558/568 is a fluorophore for the yellow region of the spectrum. Like other BDP dyes, this fluorophore has high fluorescence quantum yield, brightness, and photostability.
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Cat. # Quantity Price Lead time
17420 1 mg $110.00 in stock
27420 5 mg $210.00 in stock
47420 25 mg $410.00 in stock
57420 50 mg $695.00 in stock
67420 100 mg $1190.00 in stock

BDP 564/570 NHS ester

Lypohilic borodipyrromethene dye with orange emission for the labeling of primary and secondary amine groups.
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Cat. # Quantity Price Lead time
1H420 1 mg $110.00 in stock
2H420 5 mg $210.00 in stock
4H420 25 mg $410.00 in stock
5H420 50 mg $695.00 in stock
6H420 100 mg $1190.00 in stock

BDP 576/589 NHS ester

BDP 576/589 NHS ester (BODIPY™ 576/589 analog) is a bright hydrophobic dye with emission in the orange spectrum range. It can be used in fluorescence anisotropy assays.
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Cat. # Quantity Price Lead time
1G420 1 mg $110.00 in stock
2G420 5 mg $210.00 in stock
4G420 25 mg $410.00 in stock
5G420 50 mg $695.00 in stock
6G420 100 mg $1190.00 in stock

BDP 581/591 NHS ester

NHS ester derivative of BDP 581/591 borondipyrromethene fluorophore for the labeling of proteins, peptides, and other biomolecules. The dye is useful for fluorescence polarization and two-photon experiments, and as a ROS probe.
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Cat. # Quantity Price Lead time
16420 1 mg $110.00 in stock
26420 5 mg $210.00 in stock
46420 25 mg $410.00 in stock
56420 50 mg $695.00 in stock
66420 100 mg $1190.00 in stock

BDP 630/650 X NHS ester

BDP 630/650 is a borondipyrromethene fluorophore tuned to Cyanine5 excitation and emission channels. The dye is bright and highly photostable. It is useful for fluorescence polarization assays This is an amine-reactive NHS ester derivative.
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Cat. # Quantity Price Lead time
15420 1 mg $110.00 in stock
25420 5 mg $210.00 in stock
45420 25 mg $410.00 in stock
55420 50 mg $695.00 in stock
65420 100 mg $1190.00 in stock