Primary or secondary amino groups are ubiquitous in biomolecules. They are present in proteins and peptides as ε-amino groups of lysines and N-terminal amino groups. Many natural and synthetic small molecules are amines, and DNAs with amino groups can be prepared by solid phase synthesis, and enzymatically.
Amino groups can be modified by acylation with various activated carbonyl compounds. Among these compounds, activated N-hydroxysuccinimide esters are most suitable for biolabeling and bioconjugation applications.
Compared to other activated esters, NHS derivatives possess good stability in aqueous environment. Optimal pH for the coupling of amines with NHS esters in aqueous buffers is 8.3-8.5. Labeling is also possible in organic solvents. Polar aprotic solents, such as DMF or DMSO, are preferred. One equivalent of tertiary amine base, such as triethylamine, or diisopropylethylamine (DIPEA) is necessary to achieve efficient amine acylation in non-aqueous solution.
Ready to use kit for the labeling of antibodies (other proteins can be labeled as well) with AF488 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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Ready to use kit for the labeling of antibodies (other proteins can be labeled as well) with sulfo-Cyanine3 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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A kit for the labeling of antibodies with sulfo-Cyanine5 dye, a fluorophore for Cy5 channel.
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A kit for the labeling of antibodies with sulfo-Cyanine5.5 dye, a fluorophore for Cy5.5 channel.
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A kit for the labeling of antibodies with sulfo-Cyanine7 dye, a fluorophore for Cy7 channel.
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A kit for the labeling of antibodies with sulfo-Cyanine7.5 dye, a fluorophore for Cy7.5 channel.
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AF430 is a hydrophilic coumarin dye with the emission in green-yellow range. The NHS ester is used for the labeling of amine groups.
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10820
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AF488 NHS ester, a bright, photostable and hydrophilic fluorescent dye for green channel.
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AF568 derivative for labeling proteins and other high- and low-molecular weight ligands with free amines.
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14820
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Red-fluorescent NHS-ester of AF594 dye.
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2825-5mg
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Bright, water-soluble succinimide ester AF647 for labeling proteins and antibodies.
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56820
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Alkyne NHS ester for the labeling of biomolecules with alkyne group for Copper catalyzed Click chemistry.
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41720
25 mg
$95.00
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91720
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Bifunctional hyrophilic linker with NHS ester and terminal alkyne functional group based on triethyleneglycol (PEG3).
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2298-1g
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Activated ester for azido labeling of peptides and proteins.
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BDP 558/568 is a fluorophore for the yellow region of the spectrum. Like other BDP dyes, this fluorophore has high fluorescence quantum yield, brightness, and photostability.
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5 mg
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25 mg
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50 mg
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100 mg
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Lypohilic borodipyrromethene dye with orange emission for the labeling of primary and secondary amine groups.
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1H420
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BDP 576/589 NHS ester (BODIPY™ 576/589 analog) is a bright hydrophobic dye with emission in the orange spectrum range. It can be used in fluorescence anisotropy assays.
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NHS ester derivative of BDP 581/591 borondipyrromethene fluorophore for the labeling of proteins, peptides, and other biomolecules. The dye is useful for fluorescence polarization and two-photon experiments, and as a ROS probe.
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5 mg
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50 mg
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BDP 630/650 is a borondipyrromethene fluorophore tuned to Cyanine5 excitation and emission channels. The dye is bright and highly photostable. It is useful for fluorescence polarization assays This is an amine-reactive NHS ester derivative.
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1 mg
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5 mg
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25 mg
$410.00
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55420
50 mg
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100 mg
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Amine reactive of BDP 650/665, a borondipyrromethene dye for Cy5 channel, possessing a high molar extinction coefficient, and a good emission quantum yield.
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1E420
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Amino-reactive BDP FL, a very bright and photostable dye for fluorescein channel.
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5 mg
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50 mg
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BDP R6G is a borondipyrromethene dye which has absorption and emission spectra similar to R6G.
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Amine reactive NHS ester of BDP TMR, bright borondipyrromethene dye for TAMRA channel especially suitable for fluorescence polarization assays.
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50 mg
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BDP TR is a borondipyrromethene dye. A bright and photostable fluorophore with high quantum yield.
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BDP TR X NHS ester is a derivative of BDP TR containing a long linker based on aminohexanoic acid (C6). The dye has absorption and emission close to ROX. The NHS ester function can be used for the conjugation with proteins and peptides.
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Coumarin 343 is a blue emitting dye that can serve as a FRET donor for fluorescein (FAM). This structure features a linker between Coumarin 343 fluorophore, and the functional group. It provides better solubility, and spatial separation between the dye, and the functional group.
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Cyanine2 NHS ester minimal dye for protein labeling
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1A041
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Amine-reactive Cyanine3 dye NHS ester.
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51020
50 mg
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100 mg
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Cyanine3 minimal dye for protein labeling.
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1B041
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Cyanine3.5 dye NHS ester derivative.
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