NHS esters in Reactive groups

Primary or secondary amino groups are ubiquitous in biomolecules. They are present in proteins and peptides as ε-amino groups of lysines and N-terminal amino groups. Many natural and synthetic small molecules are amines, and DNAs with amino groups can be prepared by solid phase synthesis, and enzymatically.

Amino groups can be modified by acylation with various activated carbonyl compounds. Among these compounds, activated N-hydroxysuccinimide esters are most suitable for biolabeling and bioconjugation applications.

NHS ester coupling chemistry

Compared to other activated esters, NHS derivatives possess good stability in aqueous environment. Optimal pH for the coupling of amines with NHS esters in aqueous buffers is 8.3-8.5. Labeling is also possible in organic solvents. Polar aprotic solents, such as DMF or DMSO, are preferred. One equivalent of tertiary amine base, such as triethylamine, or diisopropylethylamine (DIPEA) is necessary to achieve efficient amine acylation in non-aqueous solution.

AF488 antibody labeling kit

Ready to use kit for the labeling of antibodies (other proteins can be labeled as well) with AF488 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
Show pricing
Cat. # Quantity Price Lead time
1821-10rxn 10 rxn $440.00 in stock

sulfo-Cyanine3 antibody labeling kit

Ready to use kit for the labeling of antibodies (other proteins can be labeled as well) with sulfo-Cyanine3 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
Show pricing
Cat. # Quantity Price Lead time
1321-10rxn 10 rxn $440.00 in stock

sulfo-Cyanine5 antibody labeling kit

A kit for the labeling of antibodies with sulfo-Cyanine5 dye, a fluorophore for Cy5 channel.
Show pricing
Cat. # Quantity Price Lead time
3321-10rxn 10 rxn $390.00 in stock

sulfo-Cyanine5.5 antibody labeling kit

A kit for the labeling of antibodies with sulfo-Cyanine5.5 dye, a fluorophore for Cy5.5 channel.
Show pricing
Cat. # Quantity Price Lead time
7321-10rxn 10 rxn $440.00 in stock

sulfo-Cyanine7 antibody labeling kit

A kit for the labeling of antibodies with sulfo-Cyanine7 dye, a fluorophore for Cy7 channel.
Show pricing
Cat. # Quantity Price Lead time
5321-10rxn 10 rxn $440.00 in stock

sulfo-Cyanine7.5 antibody labeling kit

A kit for the labeling of antibodies with sulfo-Cyanine7.5 dye, a fluorophore for Cy7.5 channel.
Show pricing
Cat. # Quantity Price Lead time
6321-10rxn 10 rxn $440.00 in stock

AF430 NHS ester

AF430 is a hydrophilic coumarin dye with the emission in green-yellow range. The NHS ester is used for the labeling of amine groups.
Show pricing
Cat. # Quantity Price Lead time
10820 1 mg $110.00 in stock
20820 5 mg $165.00 in stock
40820 25 mg $380.00 in stock
50820 50 mg $695.00 in stock
60820 100 mg $1190.00 in stock

AF488 NHS ester

AF488 NHS ester, a bright, photostable and hydrophilic fluorescent dye for green channel.
Show pricing
Cat. # Quantity Price Lead time
11820 1 mg $110.00 in stock
21820 5 mg $290.00 in stock
41820 25 mg $690.00 in stock
51820 50 mg $1270.00 in stock
61820 100 mg $1990.00 in stock

AF568 NHS ester

AF568 derivative for labeling proteins and other high- and low-molecular weight ligands with free amines.
Show pricing
Cat. # Quantity Price Lead time
14820 1 mg $110.00 5 days
24820 5 mg $290.00 in stock
44820 25 mg $690.00 in stock
54820 50 mg $1270.00 in stock
64820 100 mg $1990.00 in stock

AF594 NHS ester, 5-isomer

Red-fluorescent NHS-ester of AF594 dye.
Show pricing
Cat. # Quantity Price Lead time
2825-5mg 5 mg $355.00 in stock
2825-25mg 25 mg $1150.00 in stock
2825-50mg 50 mg $1955.00 in stock
2825-100mg 100 mg $2425.00 in stock

AF647 NHS ester

Bright, water-soluble succinimide ester AF647 for labeling proteins and antibodies.
Show pricing
Cat. # Quantity Price Lead time
16820 1 mg $110.00 in stock
26820 5 mg $340.00 in stock
46820 25 mg $1100.00 7 days
56820 50 mg $1890.00 in stock
66820 100 mg $2990.00 in stock

Alkyne NHS ester (hexynoic acid NHS ester)

Alkyne NHS ester for the labeling of biomolecules with alkyne group for Copper catalyzed Click chemistry.
Show pricing
Cat. # Quantity Price Lead time
41720 25 mg $95.00 5 days
51720 50 mg $180.00 in stock
61720 100 mg $290.00 in stock
91720 1 g please inquire in stock

Alkyne-PEG3-NHS ester

Bifunctional hyrophilic linker with NHS ester and terminal alkyne functional group based on triethyleneglycol (PEG3).
Show pricing
Cat. # Quantity Price Lead time
2298-1g 1 g $490.00 in stock
2298-5g 5 g $980.00 21 days
2298-25g 25 g please inquire 21 days

Azidobutyric acid NHS ester

Activated ester for azido labeling of peptides and proteins.
Show pricing
Cat. # Quantity Price Lead time
53720 50 mg $60.00 in stock
63720 100 mg $95.00 in stock
73720 1 g please inquire 5 days
83720 5 g please inquire in stock

BDP 558/568 NHS ester

BDP 558/568 is a fluorophore for the yellow region of the spectrum. Like other BDP dyes, this fluorophore has high fluorescence quantum yield, brightness, and photostability.
Show pricing
Cat. # Quantity Price Lead time
17420 1 mg $110.00 in stock
27420 5 mg $210.00 in stock
47420 25 mg $410.00 in stock
57420 50 mg $695.00 in stock
67420 100 mg $1190.00 in stock

BDP 564/570 NHS ester

Lypohilic borodipyrromethene dye with orange emission for the labeling of primary and secondary amine groups.
Show pricing
Cat. # Quantity Price Lead time
1H420 1 mg $110.00 in stock
2H420 5 mg $210.00 in stock
4H420 25 mg $410.00 in stock
5H420 50 mg $695.00 in stock
6H420 100 mg $1190.00 in stock

BDP 576/589 NHS ester

BDP 576/589 NHS ester (BODIPY™ 576/589 analog) is a bright hydrophobic dye with emission in the orange spectrum range. It can be used in fluorescence anisotropy assays.
Show pricing
Cat. # Quantity Price Lead time
1G420 1 mg $110.00 in stock
2G420 5 mg $210.00 in stock
4G420 25 mg $410.00 in stock
5G420 50 mg $695.00 in stock
6G420 100 mg $1190.00 in stock

BDP 581/591 NHS ester

NHS ester derivative of BDP 581/591 borondipyrromethene fluorophore for the labeling of proteins, peptides, and other biomolecules. The dye is useful for fluorescence polarization and two-photon experiments, and as a ROS probe.
Show pricing
Cat. # Quantity Price Lead time
16420 1 mg $110.00 in stock
26420 5 mg $210.00 in stock
46420 25 mg $410.00 in stock
56420 50 mg $695.00 in stock
66420 100 mg $1190.00 in stock

BDP 630/650 X NHS ester

BDP 630/650 is a borondipyrromethene fluorophore tuned to Cyanine5 excitation and emission channels. The dye is bright and highly photostable. It is useful for fluorescence polarization assays This is an amine-reactive NHS ester derivative.
Show pricing
Cat. # Quantity Price Lead time
15420 1 mg $110.00 in stock
25420 5 mg $210.00 in stock
45420 25 mg $410.00 in stock
55420 50 mg $695.00 in stock
65420 100 mg $1190.00 in stock

BDP 650/665 X NHS ester

Amine reactive of BDP 650/665, a borondipyrromethene dye for Cy5 channel, possessing a high molar extinction coefficient, and a good emission quantum yield.
Show pricing
Cat. # Quantity Price Lead time
1E420 1 mg $110.00 in stock
2E420 5 mg $210.00 in stock
4E420 25 mg $410.00 in stock
5E420 50 mg $695.00 in stock
6E420 100 mg $1190.00 in stock

BDP FL NHS ester

Amino-reactive BDP FL, a very bright and photostable dye for fluorescein channel.
Show pricing
Cat. # Quantity Price Lead time
11420 1 mg $110.00 in stock
21420 5 mg $210.00 in stock
41420 25 mg $410.00 in stock
51420 50 mg $695.00 in stock
61420 100 mg $1190.00 in stock

BDP R6G NHS ester

BDP R6G is a borondipyrromethene dye which has absorption and emission spectra similar to R6G.
Show pricing
Cat. # Quantity Price Lead time
14420 1 mg $110.00 in stock
24420 5 mg $210.00 in stock
44420 25 mg $410.00 in stock
54420 50 mg $695.00 in stock
64420 100 mg $1190.00 in stock

BDP TMR NHS ester

Amine reactive NHS ester of BDP TMR, bright borondipyrromethene dye for TAMRA channel especially suitable for fluorescence polarization assays.
Show pricing
Cat. # Quantity Price Lead time
12420 1 mg $110.00 in stock
22420 5 mg $210.00 in stock
42420 25 mg $410.00 in stock
52420 50 mg $695.00 in stock
62420 100 mg $1190.00 in stock

BDP TR NHS ester

BDP TR is a borondipyrromethene dye. A bright and photostable fluorophore with high quantum yield.
Show pricing
Cat. # Quantity Price Lead time
13420 1 mg $110.00 in stock (1 pcs)
53420 50 mg $695.00 in stock (2 pcs)
63420 100 mg $1190.00 in stock (2 pcs)

BDP TR X NHS ester

BDP TR X NHS ester is a derivative of BDP TR containing a long linker based on aminohexanoic acid (C6). The dye has absorption and emission close to ROX. The NHS ester function can be used for the conjugation with proteins and peptides.
Show pricing
Cat. # Quantity Price Lead time
1D420 1 mg $110.00 in stock
2D420 5 mg $210.00 in stock
4D420 25 mg $410.00 in stock
5D420 50 mg $695.00 in stock
6D420 100 mg $1190.00 in stock

Coumarin 343 X NHS ester

Coumarin 343 is a blue emitting dye that can serve as a FRET donor for fluorescein (FAM). This structure features a linker between Coumarin 343 fluorophore, and the functional group. It provides better solubility, and spatial separation between the dye, and the functional group.
Show pricing
Cat. # Quantity Price Lead time
12620 1 mg $110.00 in stock
22620 5 mg $210.00 in stock
42620 25 mg $410.00 in stock
52620 50 mg $695.00 in stock
62620 100 mg $1190.00 in stock

Cyanine2 NHS ester minimal dye

Cyanine2 NHS ester minimal dye for protein labeling
Show pricing
Cat. # Quantity Price Lead time
1A041 5 nmol $85.00 in stock
2A041 10 nmol $140.00 in stock
3A041 25 nmol $210.00 in stock

Cyanine3 NHS ester

Amine-reactive Cyanine3 dye NHS ester.
Show pricing
Cat. # Quantity Price Lead time
11020 1 mg $110.00 in stock
21020 5 mg $210.00 in stock
41020 25 mg $410.00 in stock
51020 50 mg $695.00 in stock
61020 100 mg $1190.00 5 days

Cyanine3 NHS ester minimal dye

Cyanine3 minimal dye for protein labeling.
Show pricing
Cat. # Quantity Price Lead time
1B041 5 nmol $85.00 in stock
2B041 10 nmol $140.00 in stock
3B041 25 nmol $210.00 in stock

Cyanine3.5 NHS ester

Cyanine3.5 dye NHS ester derivative.
Show pricing
Cat. # Quantity Price Lead time
12020 1 mg $110.00 in stock
22020 5 mg $210.00 in stock
42020 25 mg $410.00 in stock
52020 50 mg $695.00 in stock
62020 100 mg $1190.00 in stock

Cyanine5 NHS ester

Amine-reactive Cyanine5 activated ester for the labeling of proteins, peptides, and other molecules.
Show pricing
Cat. # Quantity Price Lead time
13020 1 mg $110.00 in stock
23020 5 mg $210.00 in stock
43020 25 mg $410.00 in stock
53020 50 mg $695.00 in stock
63020 100 mg $1190.00 in stock