NHS esters in Reactive groups
Primary or secondary amino groups are ubiquitous in biomolecules. They are present in proteins and peptides as ε-amino groups of lysines and N-terminal amino groups. Many natural and synthetic small molecules are amines, and DNAs with amino groups can be prepared by solid phase synthesis, and enzymatically.
Amino groups can be modified by acylation with various activated carbonyl compounds. Among these compounds, activated N-hydroxysuccinimide esters are most suitable for biolabeling and bioconjugation applications.
Compared to other activated esters, NHS derivatives possess good stability in aqueous environment. Optimal pH for the coupling of amines with NHS esters in aqueous buffers is 8.3-8.5. Labeling is also possible in organic solvents. Polar aprotic solents, such as DMF or DMSO, are preferred. One equivalent of tertiary amine base, such as triethylamine, or diisopropylethylamine (DIPEA) is necessary to achieve efficient amine acylation in non-aqueous solution.
AF488 antibody labeling kit
Ready to use kit for the labeling of antibodies (other proteins can be labeled as well) with AF488 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1821-10rxn |
10 rxn
|
$450.00
|
in stock |
sulfo-Cyanine3 antibody labeling kit
Ready to use kit for the labeling of antibodies (other proteins can be labeled as well) with sulfo-Cyanine3 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1321-10rxn |
10 rxn
|
$450.00
|
in stock |
sulfo-Cyanine5 antibody labeling kit
A kit for the labeling of antibodies with sulfo-Cyanine5 dye, a fluorophore for Cy5 channel.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3321-10rxn |
10 rxn
|
$400.00
|
in stock |
sulfo-Cyanine5.5 antibody labeling kit
A kit for the labeling of antibodies with sulfo-Cyanine5.5 dye, a fluorophore for Cy5.5 channel.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 7321-10rxn |
10 rxn
|
$450.00
|
in stock |
sulfo-Cyanine7 antibody labeling kit
A kit for the labeling of antibodies with sulfo-Cyanine7 dye, a fluorophore for Cy7 channel.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 5321-10rxn |
10 rxn
|
$450.00
|
in stock |
sulfo-Cyanine7.5 antibody labeling kit
A kit for the labeling of antibodies with sulfo-Cyanine7.5 dye, a fluorophore for Cy7.5 channel.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 6321-10rxn |
10 rxn
|
$450.00
|
in stock |
(5,6)-FAM diacetate NHS ester
Amine-reactive fluorescein (FAM) derivative, a mixture of 5- and 6-isomers. The reagent becomes brightly green fluorescent once it is hydrolyzed while diffusing into cells. A fluorescent tracer.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2934-25mg | 25 mg | $170.00 | in stock | |
| 2934-50mg | 50 mg | $270.00 | in stock | |
| 2934-100mg | 100 mg |
$520.00
|
in stock |
AF430 NHS ester
AF430 is a hydrophilic coumarin dye with the emission in green-yellow range. The NHS ester is used for the labeling of amine groups.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 10820 | 1 mg | $110.00 | in stock | |
| 20820 | 5 mg | $165.00 | in stock | |
| 40820 | 25 mg | $380.00 | in stock | |
| 50820 | 50 mg |
$695.00
|
in stock | |
| 60820 | 100 mg |
$1190.00
|
in stock |
AF488 NHS ester
AF488 NHS ester, a bright, photostable and hydrophilic fluorescent dye for green channel.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11820 | 1 mg | $110.00 | in stock | |
| 21820 | 5 mg | $290.00 | in stock | |
| 31820 | 10 mg |
$470.00
|
in stock | |
| 41820 | 25 mg |
$690.00
|
in stock | |
| 51820 | 50 mg |
$1270.00
|
in stock | |
| 61820 | 100 mg |
$1990.00
|
in stock |
AF568 NHS ester
AF568 derivative for labeling proteins and other high- and low-molecular weight ligands with free amines.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 14820 | 1 mg | $110.00 | in stock | |
| 24820 | 5 mg | $290.00 | in stock | |
| 44820 | 25 mg |
$690.00
|
in stock | |
| 54820 | 50 mg |
$1270.00
|
in stock | |
| 64820 | 100 mg |
$1990.00
|
in stock |
AF594 NHS ester, 5-isomer
Red-fluorescent NHS-ester of AF594 dye.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2825-1mg | 1 mg | $120.00 | in stock | |
| 2825-5mg | 5 mg | $355.00 | in stock | |
| 2825-25mg | 25 mg |
$1150.00
|
in stock | |
| 2825-50mg | 50 mg |
$1955.00
|
in stock | |
| 2825-100mg | 100 mg |
$2425.00
|
in stock |
AF647 NHS ester
Bright, water-soluble succinimide ester AF647 for labeling proteins and antibodies.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 16820 | 1 mg | $110.00 | 5 days | |
| 26820 | 5 mg | $340.00 | 5 days | |
| 46820 | 25 mg |
$1100.00
|
5 days | |
| 56820 | 50 mg |
$1890.00
|
in stock | |
| 66820 | 100 mg |
$2990.00
|
10 days |
Alkyne NHS ester (hexynoic acid NHS ester)
Alkyne NHS ester for the labeling of biomolecules with alkyne group for copper-catalyzed click chemistry.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 41720 | 25 mg | $95.00 | in stock | |
| 51720 | 50 mg | $180.00 | in stock | |
| 61720 | 100 mg | $290.00 | in stock | |
| 91720 | 1 g |
please inquire
|
in stock |
Alkyne-PEG3-NHS ester
Bifunctional hyrophilic linker with NHS ester and terminal alkyne functional group based on triethyleneglycol (PEG3).
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2298-1g | 1 g |
$490.00
|
in stock | |
| 2298-5g | 5 g |
$980.00
|
21 days | |
| 2298-25g | 25 g |
please inquire
|
21 days |
Azidobutyric acid NHS ester
Activated ester for azido labeling of peptides and proteins.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 53720 | 50 mg | $60.00 | in stock | |
| 63720 | 100 mg | $95.00 | in stock | |
| 73720 | 1 g |
please inquire
|
in stock | |
| 83720 | 5 g |
please inquire
|
in stock |
BDP 558/568 NHS ester
BDP 558/568 is a fluorophore for the yellow region of the spectrum. Like other BDP dyes, this fluorophore has high fluorescence quantum yield, brightness, and photostability.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 17420 | 1 mg | $110.00 | in stock | |
| 27420 | 5 mg | $210.00 | in stock | |
| 47420 | 25 mg |
$410.00
|
in stock | |
| 57420 | 50 mg |
$695.00
|
in stock | |
| 67420 | 100 mg |
$1190.00
|
in stock |
BDP 564/570 NHS ester
Lypohilic borodipyrromethene dye with orange emission for the labeling of primary and secondary amine groups.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1H420 | 1 mg | $110.00 | in stock | |
| 2H420 | 5 mg | $210.00 | in stock | |
| 4H420 | 25 mg |
$410.00
|
in stock | |
| 5H420 | 50 mg |
$695.00
|
in stock | |
| 6H420 | 100 mg |
$1190.00
|
in stock |
BDP 576/589 NHS ester
BDP 576/589 NHS ester (BODIPY™ 576/589 analog) is a bright hydrophobic dye with emission in the orange spectrum range. It can be used in fluorescence anisotropy assays.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1G420 | 1 mg | $110.00 | in stock | |
| 2G420 | 5 mg | $210.00 | in stock | |
| 4G420 | 25 mg |
$410.00
|
in stock | |
| 5G420 | 50 mg |
$695.00
|
in stock | |
| 6G420 | 100 mg |
$1190.00
|
5 days |
BDP 581/591 NHS ester
NHS ester derivative of BDP 581/591 borondipyrromethene fluorophore for the labeling of proteins, peptides, and other biomolecules. The dye is useful for fluorescence polarization and two-photon experiments, and as a ROS probe.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 16420 | 1 mg | $110.00 | in stock | |
| 26420 | 5 mg | $210.00 | in stock | |
| 46420 | 25 mg |
$410.00
|
in stock | |
| 56420 | 50 mg |
$695.00
|
in stock | |
| 66420 | 100 mg |
$1190.00
|
in stock |
BDP 630/650 X NHS ester
BDP 630/650 is a borondipyrromethene fluorophore tuned to Cyanine5 excitation and emission channels. The dye is bright and highly photostable. It is useful for fluorescence polarization assays This is an amine-reactive NHS ester derivative.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 15420 | 1 mg | $110.00 | in stock | |
| 25420 | 5 mg | $210.00 | in stock | |
| 45420 | 25 mg |
$410.00
|
in stock | |
| 55420 | 50 mg |
$695.00
|
in stock | |
| 65420 | 100 mg |
$1190.00
|
in stock |
BDP 650/665 X NHS ester
Amine reactive of BDP 650/665, a borondipyrromethene dye for Cy5 channel, possessing a high molar extinction coefficient, and a good emission quantum yield.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1E420 | 1 mg | $110.00 | in stock | |
| 2E420 | 5 mg | $210.00 | in stock | |
| 4E420 | 25 mg |
$410.00
|
in stock | |
| 5E420 | 50 mg |
$695.00
|
in stock | |
| 6E420 | 100 mg |
$1190.00
|
in stock |
BDP FL NHS ester
Amino-reactive BDP FL, a very bright and photostable dye for fluorescein channel.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11420 | 1 mg | $110.00 | in stock | |
| 21420 | 5 mg | $210.00 | in stock | |
| 41420 | 25 mg |
$410.00
|
in stock | |
| 51420 | 50 mg |
$695.00
|
in stock | |
| 61420 | 100 mg |
$1190.00
|
in stock |
BDP R6G NHS ester
BDP R6G is a borondipyrromethene dye which has absorption and emission spectra similar to R6G.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 14420 | 1 mg | $110.00 | in stock | |
| 24420 | 5 mg | $210.00 | in stock | |
| 44420 | 25 mg |
$410.00
|
in stock | |
| 54420 | 50 mg |
$695.00
|
in stock | |
| 64420 | 100 mg |
$1190.00
|
in stock |
BDP TMR NHS ester
Amine reactive NHS ester of BDP TMR, bright borondipyrromethene dye for TAMRA channel especially suitable for fluorescence polarization assays.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 12420 | 1 mg | $110.00 | in stock | |
| 22420 | 5 mg | $210.00 | in stock | |
| 42420 | 25 mg |
$410.00
|
in stock | |
| 52420 | 50 mg |
$695.00
|
in stock | |
| 62420 | 100 mg |
$1190.00
|
in stock |
BDP TR NHS ester
BDP TR is a borondipyrromethene dye. A bright and photostable fluorophore with high quantum yield.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| NO items of this product available in stock but you can submit request for bulk. | ||||
BDP TR X NHS ester
BDP TR X NHS ester is a derivative of BDP TR containing a long linker based on aminohexanoic acid (C6). The dye has absorption and emission close to ROX. The NHS ester function can be used for the conjugation with proteins and peptides.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1D420 | 1 mg | $110.00 | in stock | |
| 2D420 | 5 mg | $210.00 | in stock | |
| 4D420 | 25 mg |
$410.00
|
in stock | |
| 5D420 | 50 mg |
$695.00
|
in stock | |
| 6D420 | 100 mg |
$1190.00
|
in stock |
Biotin-X-NHS ester
Biotin derivative with C6-spacer to reduce steric effect when binding amino acids, peptides, or proteins by reacting with primary amines.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2551-100mg | 100 mg | $117.00 | in stock | |
| 2551-1g | 1 g |
please inquire
|
in stock |
Coumarin 343 X NHS ester
Coumarin 343 is a blue emitting dye that can serve as a FRET donor for fluorescein (FAM). This structure features a linker between Coumarin 343 fluorophore, and the functional group. It provides better solubility, and spatial separation between the dye, and the functional group.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 12620 | 1 mg | $110.00 | in stock | |
| 22620 | 5 mg | $210.00 | in stock | |
| 42620 | 25 mg |
$410.00
|
in stock | |
| 52620 | 50 mg |
$695.00
|
in stock | |
| 62620 | 100 mg |
$1190.00
|
in stock |
Cyanine2 NHS ester minimal dye
Cyanine2 NHS ester minimal dye for protein labeling
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1A041 | 5 nmol | $85.00 | in stock | |
| 2A041 | 10 nmol | $140.00 | in stock | |
| 3A041 | 25 nmol | $210.00 | in stock |
Cyanine3 NHS ester
Amine-reactive Cyanine3 dye NHS ester.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11020 | 1 mg | $110.00 | in stock | |
| 21020 | 5 mg | $210.00 | in stock | |
| 41020 | 25 mg |
$410.00
|
in stock | |
| 51020 | 50 mg |
$695.00
|
in stock | |
| 61020 | 100 mg |
$1190.00
|
in stock |
Cyanine3 NHS ester minimal dye
Cyanine3 minimal dye for protein labeling.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1B041 | 5 nmol | $85.00 | in stock | |
| 2B041 | 10 nmol | $140.00 | in stock | |