NHS esters in Reactive groups

Primary or secondary amino groups are ubiquitous in biomolecules. They are present in proteins and peptides as ε-amino groups of lysines and N-terminal amino groups. Many natural and synthetic small molecules are amines, and DNAs with amino groups can be prepared by solid phase synthesis, and enzymatically.

Amino groups can be modified by acylation with various activated carbonyl compounds. Among these compounds, activated N-hydroxysuccinimide esters are most suitable for biolabeling and bioconjugation applications.

Compared to other activated esters, NHS derivatives possess good stability in aqueous environment. Optimal pH for the coupling of amines with NHS esters in aqueous buffers is 8.3-8.5. Labeling is also possible in organic solvents. Polar aprotic solents, such as DMF or DMSO, are preferred. One equivalent of tertiary amine base, such as triethylamine, or diisopropylethylamine (DIPEA) is necessary to achieve efficient amine acylation in non-aqueous solution.

sulfo-Cyanine3 antibody labeling kit

Ready to use kit for the labeling of antibodies (other proteins can be labeled as well) with sulfo-Cyanine3 dye via NHS ester chemistry. The kit contains all necessary chemicals and consumables.
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Cat. # Quantity Price Lead time
1321-10rxn 10 rxn $390.00 in stock

sulfo-Cyanine5 antibody labeling kit

A kit for the labeling of antibodies with sulfo-Cyanine5 dye, a fluorophore for Cy5 channel.
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Cat. # Quantity Price Lead time
3321-10rxn 10 rxn $390.00 in stock

Alkyne NHS ester (hexynoic acid NHS ester)

Alkyne NHS ester for the labeling of biomolecules with alkyne group for Copper catalyzed Click chemistry.
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Cat. # Quantity Price Lead time
41720 25 mg $95.00 in stock
51720 50 mg $195.00 in stock
61720 100 mg $290.00 in stock

Azidobutyric acid NHS ester

Activated ester for azido labeling of peptides and proteins.
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Cat. # Quantity Price Lead time
33720 10 mg $60.00 in stock
43720 25 mg $95.00 in stock
53720 50 mg $185.00 in stock
63720 100 mg $290.00 in stock

BDP 558/568 NHS ester

BDP 558/568 is a fluorophore for the yellow region of the spectrum. Like other BDP dyes, this fluorophore has high fluorescence quantum yield, brightness, and photostability.
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Cat. # Quantity Price Lead time
17420 1 mg $110.00 in stock
27420 5 mg $210.00 in stock
47420 25 mg $410.00 in stock
57420 50 mg $695.00 in stock
67420 100 mg $1190.00 in stock

BDP 581/591 NHS ester

NHS ester derivative of BDP 581/591 borondipyrromethene fluorophore for the labeling of proteins, peptides, and other biomolecules. The dye is useful for fluorescence polarization and two-photon experiments, and as a ROS probe.
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Cat. # Quantity Price Lead time
16420 1 mg $110.00 in stock
26420 5 mg $210.00 in stock
46420 25 mg $410.00 in stock
56420 50 mg $695.00 in stock
66420 100 mg $1190.00 in stock

BDP 630/650 X NHS ester

BDP 630/650 is a borondipyrromethene fluorophore tuned to Cyanine5 excitation and emission channels. The dye is bright and highly photostable. It is useful for fluorescence polarization assays This is an amine-reactive NHS ester derivative.
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Cat. # Quantity Price Lead time
15420 1 mg $110.00 in stock
25420 5 mg $210.00 in stock
45420 25 mg $410.00 in stock
55420 50 mg $695.00 in stock
65420 100 mg $1190.00 in stock

BDP FL NHS ester

Amino-reactive BDP FL, a very bright and photostable dye for fluorescein channel.
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Cat. # Quantity Price Lead time
11420 1 mg $110.00 in stock
21420 5 mg $210.00 in stock
41420 25 mg $410.00 in stock
51420 50 mg $695.00 in stock
61420 100 mg $1190.00 in stock

BDP R6G NHS ester

BDP R6G is a borondipyrromethene dye which has absorption and emission spectra similar to R6G.
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Cat. # Quantity Price Lead time
14420 1 mg $110.00 in stock
24420 5 mg $210.00 in stock
44420 25 mg $410.00 in stock
54420 50 mg $695.00 in stock
64420 100 mg $1190.00 in stock

BDP TMR NHS ester

Amine reactive NHS ester of BDP TMR, bright borondipyrromethene dye for TAMRA channel especially suitable for fluorescence polarization assays.
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Cat. # Quantity Price Lead time
12420 1 mg $110.00 in stock
22420 5 mg $210.00 in stock
42420 25 mg $410.00 in stock
52420 50 mg $695.00 in stock
62420 100 mg $1190.00 in stock

BDP TR NHS ester

BDP TR is a borondipyrromethene dye for Texas Red channel. A bright and photostable fluorophore with high quantum yield.
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Cat. # Quantity Price Lead time
13420 1 mg $110.00 in stock
23420 5 mg $210.00 in stock
43420 25 mg $410.00 in stock
53420 50 mg $695.00 in stock
63420 100 mg $1190.00 in stock

Coumarin 343 X NHS ester

Coumarin 343 is a blue emitting dye that can serve as a FRET donor for fluorescein (FAM). This structure features a linker between Coumarin 343 fluorophore, and the functional group. It provides better solubility, and spatial separation between the dye, and the functional group.
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Cat. # Quantity Price Lead time
12620 1 mg $110.00 in stock
22620 5 mg $210.00 in stock
42620 25 mg $410.00 in stock
52620 50 mg $695.00 in stock
62620 100 mg $1190.00 in stock

Cyanine2 NHS ester minimal dye

Cyanine2 NHS ester minimal dye for protein labeling
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Cat. # Quantity Price Lead time
1A041 5 nmol $85.00 in stock
2A041 10 nmol $140.00 in stock
3A041 25 nmol $210.00 in stock

Cyanine3 NHS ester

Amine-reactive Cyanine3 dye NHS ester.
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Cat. # Quantity Price Lead time
11020 1 mg $110.00 in stock
21020 5 mg $210.00 in stock
41020 25 mg $410.00 in stock
51020 50 mg $695.00 in stock
61020 100 mg $1190.00 in stock

Cyanine3 NHS ester minimal dye

Cyanine3 minimal dye for protein labeling.
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Cat. # Quantity Price Lead time
1B041 5 nmol $85.00 in stock
2B041 10 nmol $140.00 in stock
3B041 25 nmol $210.00 in stock

Cyanine3.5 NHS ester

Cyanine3.5 dye NHS ester derivative.
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Cat. # Quantity Price Lead time
12020 1 mg $110.00 in stock
22020 5 mg $210.00 in stock
42020 25 mg $410.00 in stock
52020 50 mg $695.00 in stock
62020 100 mg $1190.00 in stock

Cyanine5 NHS ester

Amine-reactive Cyanine5 activated ester for the labeling of proteins, peptides, and other molecules.
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Cat. # Quantity Price Lead time
13020 1 mg $110.00 in stock
23020 5 mg $210.00 in stock
43020 25 mg $410.00 in stock
53020 50 mg $695.00 in stock
63020 100 mg $1190.00 in stock

Cyanine5 NHS ester minimal dye

Cy5 NHS ester minimal dye for protein labeling.
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Cat. # Quantity Price Lead time
1C041 5 nmol $85.00 in stock
2C041 10 nmol $140.00 in stock
3C041 25 nmol $210.00 in stock

Cyanine5.5 NHS ester

Cyanine5.5 NHS is far-red / near-infrared amine-reactive dye.
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Cat. # Quantity Price Lead time
17020 1 mg $110.00 in stock
27020 5 mg $210.00 in stock
47020 25 mg $410.00 in stock
57020 50 mg $695.00 in stock
67020 100 mg $1190.00 in stock

Cyanine7 NHS ester

Amine reactive NHS ester of near infrared dye Cyanine7.
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Cat. # Quantity Price Lead time
15020 1 mg $110.00 in stock
25020 5 mg $210.00 in stock
45020 25 mg $410.00 in stock
55020 50 mg $695.00 in stock
65020 100 mg $1190.00 in stock

Cyanine7.5 NHS ester

Amine reactive NHS ester of NIR fluorescent dye Cyanine7.5.
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Cat. # Quantity Price Lead time
16020 1 mg $110.00 in stock
26020 5 mg $210.00 in stock
46020 25 mg $410.00 in stock
56020 50 mg $695.00 in stock
66020 100 mg $1190.00 in stock

DBCO NHS ester

Azodibenzocyclooctyne (DBCO, ADIBO) reagent for strain promoted copper free Click chemistry (spAAC). The reagent contains an NHS ester function for the attachment of the cyclooctyne to various molecules.
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Cat. # Quantity Price Lead time
34720 10 mg $60.00 in stock
44720 25 mg $95.00 in stock
54720 50 mg $145.00 in stock
64720 100 mg $210.00 in stock
94720 1 g $1190.00 in stock

FAM NHS ester, 6-isomer

Activated N-hydroxysuccinimide ester of fluorescein (FAM), pure 6-isomer.
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Cat. # Quantity Price Lead time
15120 1 mg $110.00 in stock
25120 5 mg $210.00 in stock
45120 25 mg $160.00 in stock
55120 50 mg $270.00 in stock
65120 100 mg $420.00 in stock

Pyrenebutyric acid NHS ester

Pyrene NHS ester for the labeling of biomolecules with pyrene residue. Pyrene is a fluorescent proximity probe which forms excimers, and it is also an anchor group for graphite immobilization.
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Cat. # Quantity Price Lead time
63520 100 mg $110.00 in stock

ROX NHS ester, pure 6- isomer

Pure isomer 6-ROX NHS ester for amine labeling.
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Cat. # Quantity Price Lead time
12220 1 mg $110.00 in stock
22220 5 mg $210.00 in stock
42220 25 mg $410.00 in stock
52220 50 mg $695.00 in stock
62220 100 mg $1190.00 in stock

Sulfo-Cyanine3 NHS ester

Water soluble sulfo-Cyanine3 SE activated ester for amino-biomolecule labeling.
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Cat. # Quantity Price Lead time
11320 1 mg $110.00 in stock
21320 5 mg $290.00 in stock
41320 25 mg $690.00 in stock
51320 50 mg $1270.00 in stock
61320 100 mg $1990.00 in stock

Sulfo-Cyanine5 bis-NHS ester

Water soluble amine reactive Cyanine5 dye with two NHS ester groups.
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Cat. # Quantity Price Lead time
133D0 1 mg $110.00 in stock
233D0 5 mg $290.00 in stock
433D0 25 mg $690.00 in stock
533D0 50 mg $1270.00 in stock
633D0 100 mg $1990.00 in stock

Sulfo-Cyanine5 NHS ester

Water-soluble Cyanine 5 succinimide ester.
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Cat. # Quantity Price Lead time
13320 1 mg $110.00 in stock
23320 5 mg $290.00 in stock
43320 25 mg $690.00 in stock
53320 50 mg $1270.00 in stock
63320 100 mg $1990.00 in stock

Sulfo-Cyanine5.5 NHS ester

Water soluble, sulfonated, far-red sulfo-Cyanine5.5 dye in the form of NHS ester.
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Cat. # Quantity Price Lead time
17320 1 mg $110.00 in stock
27320 5 mg $290.00 in stock
47320 25 mg $690.00 in stock
57320 50 mg $1190.00 in stock
67320 100 mg $1850.00 in stock

Sulfo-Cyanine7 NHS ester

Sulfo-Cyanine7 NHS ester is a water soluble, near infrared, amine-reactive dye.
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Cat. # Quantity Price Lead time
15320 1 mg $110.00 in stock
25320 5 mg $290.00 in stock
45320 25 mg $690.00 in stock
55320 50 mg $1270.00 5 days
65320 100 mg $1990.00 in stock

Sulfo-Cyanine7.5 NHS ester

A water soluble near infrared emitting dye for in vivo imaging applications. Sulfo-Cyanine7.5 is an analog of indocyanine green (ICG) possessing higher emission quantum yield, and containing a linker arm.
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Cat. # Quantity Price Lead time
16320 1 mg $110.00 in stock
26320 5 mg $290.00 in stock
46320 25 mg $690.00 in stock
56320 50 mg $1190.00 in stock
66320 100 mg $1850.00 in stock

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