Cyanine3 NHS ester minimal dye
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1B041 | 5 nmol | $85.00 | in stock | |
| 2B041 | 10 nmol | $140.00 | in stock | |
| 3B041 | 25 nmol | $210.00 | in stock |
Cyanine3 NHS ester dye for protein labeling, and analog of Cy3® minimal dye.
This reagent is specially quantified - each package contains specified amount of NHS ester with quantity variation within 10%.
Recommended protocol
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Protected Cyanine5 hydrazide, a dye with a reactivity for carbonyl groups (aldehydes and ketones) activated by acid.EdU (5-ethynyl-2'-deoxyuridine)
EdU is a nucleoside which is incorporated into replicated DNA by cellular enzymes. After it, DNA contained in the cells can be developed by Click chemistry reaction with fluorescent dye azides to reveal cell proliferation.General properties
| Appearance: | red solid |
| Quality control: | NMR 1H, HPLC-MS, functional testing |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 555 |
| Emission maximum, nm: | 570 |
| CF260: | 0.04 |
| CF280: | 0.09 |
Product citations
- Laberge, A.; Ayoub, A.; Arif, S.; Larochelle, S.; Garnier, A.; Moulin, V.J. α-2-Macroglobulin induces the shedding of microvesicles from cutaneous wound myofibroblasts. Journal of Cellular Physiology, 2019, 234(7), 11369–11379. doi: 10.1002/jcp.27794
- Malheiros, J.M.; Braga, C.P.; Grove, R.A.; Ribeiro, F.A.; Calkins, C.R.; Adamec, J.; Chardulo, L.A.L. Influence of oxidative damage to proteins on meat tenderness using a proteomics approach. Meat Science, 2019, 148, 64–71. doi: 10.1016/j.meatsci.2018.08.016
- Jin, Y.N.; Schlueter, P.J.; Jurisch-Yaksi, N.; Lam, P.-Y.; Jin, S.; Hwang, W.Y.; Yeh, J.-R.J.; Yoshigi, M.; Ong, S.-E.; Schenone, M.; Hartigan, C.R.; Carr, S.A.; Peterson, R.T. Noncanonical translation via deadenylated 3' UTRs maintains primordial germ cells. Nature Chemical Biology, 2018, 14(9), 844–852. doi: 10.1038/s41589-018-0098-0
- Lu, J.; Tang, M.; Liu, Y.; Wang, J.; Wu, Z. Comparative Proteomics of Chromium-Transformed Beas-2B Cells by 2D-DIGE and MALDI-TOF/TOF MS. Biological Trace Element Research, 2018, 185(1), 78–88. doi: 10.1007/s12011-017-1222-9
Cy® is a trademark of GE Healthcare.
Purchase of these products does not imply transfer of license to perform methods patented by Carnegie Mellon University and covered by US patents 6,043,025, 6,127,134, 6,246,190, 7566,544, and 7,598,047 "Difference gel electrophoresis using matched multiple dyes" and family members in Australia, Canada, Europe and Japan.
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