ROX azide, 5- isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11230 | 100 uL, 10 mM/DMSO | $110.00 | in stock | |
| 31230 | 500 uL, 10 mM/DMSO | $210.00 | in stock | |
| 41230 | 1 mL, 10 mM/DMSO |
$410.00
|
in stock | |
| A1230 | 1 mg | $110.00 | in stock | |
| B1230 | 5 mg | $210.00 | in stock | |
| C1230 | 10 mg | $310.00 | in stock | |
| D1230 | 25 mg |
$410.00
|
in stock | |
| E1230 | 50 mg |
$695.00
|
in stock |
ROX (rhodamine-X) azide, solid material, and 10 mM solution in DMSO, labeling reagent for Click Chemistry. ROX is a red-emitting rhodamine dye possessing high brightness and fluorescence quantum yield.
Pure 5-isomer.
Can replace Alexa Fluor 568.
Absorption and emission spectra of ROX dye
Recommended protocol
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Reagent for fluorescent labeling with pyrene polycyclic aromatic hydrocarbon via Click Chemistry.JOE azide, 5- isomer
JOE is a chlorinated fluorescein dye which is often used for HEX channel for oligonucleotides. It is less hydrophobic than HEX. This is an azide derivative for Click chemistryDMSO, labeling grade
Pure dimethyl sulfoxide (DMSO) solvent with quality optimised for Click and other cojugation reactionsGeneral properties
| Appearance: | dark crimson powder / red solution |
| Molecular weight: | 616.71 |
| Molecular formula: | C36H36N6O4 |
| Solubility: | good in polar organic solvents (DMF, DMSO, alcohols), low in water |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 570 |
| ε, L⋅mol−1⋅cm−1: | 93000 |
| Emission maximum, nm: | 591 |
| Fluorescence quantum yield: | 1.0 |
Product citations
- Feltes, M.; Moores, S.; Gale, S.E.; Krishnan, K.; Mydock-McGrane, L.; Covey, D.F.; Ory, D.S.; Schaffer, J.E. Synthesis and characterization of diazirine alkyne probes for the study of intracellular cholesterol trafficking. Journal of Lipid Research, 2019, 60(3), 707–716. doi: 10.1194/jlr.D091470
- Verardi, R.; Kim, J.-S.; Ghirlando, R.; Banerjee, A. Structural Basis for Substrate Recognition by the Ankyrin Repeat Domain of Human DHHC17 Palmitoyltransferase. Structure, 2017, 25(9), 1337–1347. doi: 10.1016/j.str.2017.06.018
- Bak, D.W.; Pizzagalli, M.D.; Weerapana, E. Identifying Functional Cysteine Residues in the Mitochondria. ACS Chemical Biology, 2017, 12(4), 947–957. doi: 10.1021/acschembio.6b01074
- Nåbo, L.J.; Madsen, C.S.; Jensen, K.J.; Kongsted, J.; Astakhova, K. Ultramild Protein-Mediated Click Chemistry Creates Efficient Oligonucleotide Probes for Targeting and Detecting Nucleic Acids. ChemBioChem, 2015, 16(8), 1163–1167. doi: 10.1002/cbic.201500145
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