ROX azide, 5- isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11230 | 100 uL, 10 mM/DMSO | $110.00 | in stock | |
| 31230 | 500 uL, 10 mM/DMSO | $210.00 | in stock | |
| 41230 | 1 mL, 10 mM/DMSO |
$410.00
|
in stock | |
| A1230 | 1 mg | $110.00 | in stock | |
| B1230 | 5 mg | $210.00 | in stock | |
| C1230 | 10 mg | $310.00 | in stock | |
| D1230 | 25 mg |
$410.00
|
in stock | |
| E1230 | 50 mg |
$695.00
|
in stock |
ROX (rhodamine-X) azide, solid material, and 10 mM solution in DMSO, labeling reagent for Click Chemistry. ROX is a red-emitting rhodamine dye possessing high brightness and fluorescence quantum yield.
Pure 5-isomer.
Can replace Alexa Fluor 568.
Absorption and emission spectra of ROX dye
Recommended protocol
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ROX NHS ester, 5-isomer
Activated NHS ester of ROX (rhodamine X or rhodamine 101), pure 5-isomer, for the labeling of amine groups of biomolecules.
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dsGreen Gel Staining Solution, 10000×
dsGreen, a SYBR Green I analog for staining dsDNA in gelsBDP TMR NHS ester
Amine reactive NHS ester of BDP TMR, bright borondipyrromethene dye for TAMRA channel especially suitable for fluorescence polarization assays.General properties
| Appearance: | dark crimson powder / red solution |
| Molecular weight: | 616.71 |
| Molecular formula: | C36H36N6O4 |
| Solubility: | good in polar organic solvents (DMF, DMSO, alcohols), low in water |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 570 |
| ε, L⋅mol−1⋅cm−1: | 93000 |
| Emission maximum, nm: | 591 |
| Fluorescence quantum yield: | 1.0 |
Product citations
- Feltes, M.; Moores, S.; Gale, S.E.; Krishnan, K.; Mydock-McGrane, L.; Covey, D.F.; Ory, D.S.; Schaffer, J.E. Synthesis and characterization of diazirine alkyne probes for the study of intracellular cholesterol trafficking. Journal of Lipid Research, 2019, 60(3), 707–716. doi: 10.1194/jlr.D091470
- Verardi, R.; Kim, J.-S.; Ghirlando, R.; Banerjee, A. Structural Basis for Substrate Recognition by the Ankyrin Repeat Domain of Human DHHC17 Palmitoyltransferase. Structure, 2017, 25(9), 1337–1347. doi: 10.1016/j.str.2017.06.018
- Bak, D.W.; Pizzagalli, M.D.; Weerapana, E. Identifying Functional Cysteine Residues in the Mitochondria. ACS Chemical Biology, 2017, 12(4), 947–957. doi: 10.1021/acschembio.6b01074
- Nåbo, L.J.; Madsen, C.S.; Jensen, K.J.; Kongsted, J.; Astakhova, K. Ultramild Protein-Mediated Click Chemistry Creates Efficient Oligonucleotide Probes for Targeting and Detecting Nucleic Acids. ChemBioChem, 2015, 16(8), 1163–1167. doi: 10.1002/cbic.201500145
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