ROX azide, 5- isomer

ROX (Rhodamine X, Rhodamine 101) Azides Microscopy
Cat. # Quantity Price Lead time
11230 100 uL, 10 mM/DMSO $110.00 in stock
31230 500 uL, 10 mM/DMSO $210.00 in stock
41230 1 mL, 10 mM/DMSO $410.00 in stock
A1230 1 mg $110.00 in stock
B1230 5 mg $210.00 in stock
C1230 10 mg $310.00 in stock
D1230 25 mg $410.00 in stock
E1230 50 mg $695.00 in stock
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ROX (rhodamine-X) azide, solid material, and 10 mM solution in DMSO, labeling reagent for Click Chemistry. ROX is a red-emitting rhodamine dye possessing high brightness and fluorescence quantum yield.

Pure 5-isomer.

Can replace Alexa Fluor 568.

ROX azide, 5- isomer, D1230
ROX azide, 5- isomer, D1230
ROX azide, 5- isomer, x1230
ROX azide, 5- isomer, 10 mM/DMSO, 41230
ROX azide, 5- isomer, 10 mM/DMSO, 41230
ROX azide, 5- isomer, 10 mM/DMSO, 41230
ROX azide, 5- isomer, 10 mM/DMSO, x1230
Structure of ROX azide, 5-isomer
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Absorption and emission spectra of ROX dye

Absorption and emission spectra of ROX dye

Recommended protocol

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ROX NHS ester, 6- isomer

Pure isomer 6-ROX NHS ester for amine labeling.

Sulfo-Cyanine5.5 NHS ester

Water soluble, sulfonated, far-red sulfo-Cyanine5.5 dye in the form of NHS ester.

DBCO NHS ester

Azodibenzocyclooctyne (DBCO, ADIBO) reagent for strain promoted copper free Click chemistry (spAAC). The reagent contains an NHS ester function for the attachment of the cyclooctyne to various molecules.

General properties

Appearance: dark crimson powder / red solution
Molecular weight: 616.71
Molecular formula: C36H36N6O4
Solubility: good in polar organic solvents (DMF, DMSO, alcohols), low in water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 570
ε, L⋅mol−1⋅cm−1: 93000
Emission maximum, nm: 591
Fluorescence quantum yield: 1.0

Product citations

  1. Feltes, M.; Moores, S.; Gale, S.E.; Krishnan, K.; Mydock-McGrane, L.; Covey, D.F.; Ory, D.S.; Schaffer, J.E. Synthesis and characterization of diazirine alkyne probes for the study of intracellular cholesterol trafficking. Journal of Lipid Research, 2019, 60(3), 707–716. doi: 10.1194/jlr.D091470
  2. Verardi, R.; Kim, J.-S.; Ghirlando, R.; Banerjee, A. Structural Basis for Substrate Recognition by the Ankyrin Repeat Domain of Human DHHC17 Palmitoyltransferase. Structure, 2017, 25(9), 1337–1347. doi: 10.1016/j.str.2017.06.018
  3. Bak, D.W.; Pizzagalli, M.D.; Weerapana, E. Identifying Functional Cysteine Residues in the Mitochondria. ACS Chemical Biology, 2017, 12(4), 947–957. doi: 10.1021/acschembio.6b01074
  4. Nåbo, L.J.; Madsen, C.S.; Jensen, K.J.; Kongsted, J.; Astakhova, K. Ultramild Protein-Mediated Click Chemistry Creates Efficient Oligonucleotide Probes for Targeting and Detecting Nucleic Acids. ChemBioChem, 2015, 16(8), 1163–1167. doi: 10.1002/cbic.201500145
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