FAM phosphoramidite, 5-isomer

Oligonucleotide synthesis FAM (Fluorescein) Phosphoramidites

Cat. #	Quantity	Price	Lead time
2639-500mg / Easily soluble lyophilised form in 8 mL septum	500 mg	–	in stock
2639-1g / Easily soluble lyophilised form in 20 mL septum	1 g	$190 $147	in stock
2639-5g / in 30 mL septum	5 g	$740 $629	in stock
2639-10g / in 30 mL septum	10 g	$1290 $970	in stock
2639-50g	50 g	please inquire	21 days
2639-100g	100 g	please inquire	21 days

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Standard fluorescein (FAM) phosphoramidite for 5'-terminal oligonucleotide labeling, high isomeric purity single isomer. This highly purified amidite reagent ensures excellent coupling results with various synthesizers.

Samples and special offers are available for oligo manufacturers. Contact us for bulk supplies of this reagent.

Structure of FAM Phosphoramidite, 5-Isomer

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FAM absorbance and emission spectra

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Maleimide reagent for the attachment of alkyne functional group to proteins, peptides, and other molecules bearing SH (thiol) groups, including cysteine residues.

General properties

Appearance:	off white solid
Molecular weight:	843.94
CAS number:	147566-42-5
Molecular formula:	C₄₆H₅₈N₃O₁₀P
Solubility:	Good solubility in acetonitrile and DCM
Quality control:	NMR ¹H and ³¹P, HPLC-MS (95+%), isomeric purity > 97%
Storage conditions:	Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	492
ε, L⋅mol⁻¹⋅cm⁻¹:	72000
Emission maximum, nm:	517
Fluorescence quantum yield:	0.93
CF₂₆₀:	0.22
CF₂₈₀:	0.17

Oligo synthesis details

Diluent:	acetonitrile
Coupling conditions:	coupling time 10 min
Cleavage conditions:	ammonia, 2 h at room temperature
Deprotection conditions:	identical to protected nucleobases; when AMA is used, deblock with ammonia alone for 30 min, then add methylamine

Product citations

Zharkov, T. D.; Markov, O. V.; Zhukov, S. A.; Khodyreva, S. N.; Kupryushkin, M. S. Influence of Combinations of Lipophilic and Phosphate Backbone Modifications on Cellular Uptake of Modified Oligonucleotides. Molecules, 2024, 29(2), 452. doi: 10.3390/molecules29020452
Bauer, I.; Ilina, E.; Zharkov, T.; Grigorieva, E.; Chinak, O.; Kupryushkin, M.; Golyshev, V.; Mitin, D.; Chubarov, A.; Khodyreva, S.; Dmitrienko, E. Self-Penetrating Oligonucleotide Derivatives: Features of Self-Assembly and Interactions with Serum and Intracellular Proteins. Pharmaceutics, 2023, 15(12), 2779. doi: 10.3390/pharmaceutics15122779
Petrunina, N.A.; Lebedev, V.V.; Kirillova, Y.G.; Aralov, A..V.; Varizhuk, A.M.; Sardushkin, M.V. DNA Intercalated Motifs with Non-Nucleoside Inserts. Russian Journal of Bioorganic Chemistry, 2021, 47(6), 1341–1344. doi: 10.1134/S1068162021060212

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.