SIMA azide, 6-isomer

Cat. # Quantity Price Lead time
AE230 1 mg $110 in stock
BE230 5 mg $180 in stock
CE230 10 mg $310 in stock
DE230 25 mg $410 in stock
EE230 50 mg $695 in stock
FE230 100 mg $1190 in stock
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SIMA (dichloro-diphenyl-fluorescein) is a dye with spectral properties similar to HEX but with a higher quantum yield.

SIMA azide is used to produce fluorescently labeled primers and hybridization probes for quantitative PCR. Oligonucleotides with a SIMA label can be easily generated via azide-alkyne cycloaddition between SIMA azide and alkyne-containing oligonucleotide.

Absorption and emission spectra of SIMA

Absorption and emission spectra of SIMA

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General properties

Appearance: orange powder
Molecular weight: 679.52
Molecular formula: C36H24Cl2N4O6
Solubility: good in DMSO, DMF
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 531
ε, L⋅mol−1⋅cm−1: 92300
Emission maximum, nm: 555
Fluorescence quantum yield: 0.63
CF260: 0.57
CF280: 0.18
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