Cyanine5 azide

Cat. # Quantity Price Lead time
13030 100 uL, 10 mM/DMSO –   in stock
33030 500 uL, 10 mM/DMSO $199 in stock
43030 1 mL, 10 mM/DMSO $495 in stock
A3030 1 mg –   in stock
B3030 5 mg $199 in stock
C3030 10 mg $297 in stock
D3030 25 mg $495 in stock
E3030 50 mg $895 in stock
F3030 100 mg $1490 in stock
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Cyanine5 azide labeling reagent for Click Chemistry, available as 10 mM solution in DMSO and in solid form.

This azide is soluble in organic solvents (e.g., DMSO, DMF); therefore, the labeling reaction should be carried out with a small amount of an organic co-solvent. This azide can be used for the labeling of alkyne-modified biomolecules in mixtures of water with organic solvents. The solution in DMSO is ready for use in bioconjugation. A water-soluble sulfonated version of this reagent is also available.

Cyanine5 is an analog of Cy5®, one of the most commonly used fluorophores, which is compatible with various instruments. Cyanine5 can also be used as a replacement for DyLight® 649.

Absorbance and emission spectra of Cyanine5

Absorbance and emission spectra of Cyanine5

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General properties

Appearance: dark blue powder / solution
Molecular weight: 601.22
CAS number: 1267539-32-1 (chloride)
Molecular formula: C35H45ClN6O
Solubility: soluble in organic solvents (DMSO, DMF, dichloromethane), very poorly soluble in water (0.63 mM, 110 mg/L)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2
CF260: 0.03
CF280: 0.04

Product citations

  1. Xu, M.J.; Jordan, P.W. SMC5/6 Promotes Replication Fork Stability via Negative Regulation of the COP9 Signalosome. International Journal of Molecular Sciences, 2024, 25(2), 952. doi: 10.3390/ijms25020952
  2. Ramezani, M.; Bauman, J.; Singh, A.; Weisbart, E.; Yong, J.; Lozada, M.; Way, G.P.; Kavari, S.L.; Diaz, C.; Haghighi, M.; Batista, T.M.; Pérez-Schindler, J.; Claussnitzer, M.; Singh, S.; Cimini, B.A.; Blainey, P.C.; Carpenter, A.E.; Jan, C.H.; Neal, J.T. A genome-wide atlas of human cell morphology. bioRxiv, 2023. doi: 10.1101/2023.08.06.552164
  3. Tsuchiya, M.; Tachibana, N.; Hamachi, I. Flow cytometric analysis of phosphatidylcholine metabolism using organelle-selective click labeling. STAR Protocols, 2023, 4(3), 102525. doi: 10.1016/j.xpro.2023.102525
  4. Zhao, Y.; Miranda Herrera, P. A.; Chang, D.; Hamelin, R.; Long, M. J. C.; Aye, Y. Function-Guided Proximity Mapping Unveils Electrophilic-Metabolite Sensing by Proteins Not Present in Their Canonical Locales. Proc. Natl. Acad. Sci. U.S.A., 2022, 119(5), e2120687119. doi: 10.1073/pnas.2120687119
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