Cyanine5 azide

Cat. # Quantity Price Lead time
13030 100 uL, 10 mM/DMSO $110.00 in stock
33030 500 uL, 10 mM/DMSO $210.00 in stock
43030 1 mL, 10 mM/DMSO $410.00 in stock
A3030 1 mg $110.00 in stock
B3030 5 mg $210.00 in stock
C3030 10 mg $310.00 in stock
D3030 25 mg $410.00 in stock
E3030 50 mg $695.00 in stock
F3030 100 mg $1190.00 in stock

Cyanine5 azide labeling reagent for Click Chemistry, available as 10 mM solution in DMSO, and in solid form.

This azide is soluble in organic solvents (e.g. DMSO, DMF), therefore the labeling reaction should be carried out with a small amount of an organic co-solvent. This azide can be used for the labeling of alkyne-modified biomolecules in mixtures of water with organic solvents. The solution in DMSO is ready for use in bioconjugation. A water-soluble sulfonated version of this reagent is also available.

Cyanine5 is an analog of Cy5®, one of the most commonly used fluorophores which is compatible with various instruments. Cyanine5 can also be used as a replacement for Alexa Fluor® 647, and DyLight® 649.

Cyanine5 absorbance and emission spectra

Cyanine5 absorbance and emission spectra

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General properties

Appearance: dark blue powder / solution
Molecular weight: 601.22
CAS number: 1267539-32-1 (chloride)
Molecular formula: C35H45ClN6O
Solubility: soluble in organic solvents (DMSO, DMF, dichloromethane), very poorly soluble in water (0.63 mM, 110 mg/L)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2
CF260: 0.03
CF280: 0.04

Product citations

  1. Thauvin, C.; Maudens, P.; Allémann, E. Microwave-assisted synthesis of self-assembling bi-functionalizable amphiphilic diblock copolymers. Journal of Drug Delivery Science and Technology, 2021, 61, 102255. doi: 10.1016/j.jddst.2020.102255
  2. Boersma, S.; Rabouw, H.H.; Bruurs, L.J.M.; Pavlovič, T.; van Vliet, A.L.W.; Beumer, J.; Clevers, H.; van Kuppeveld, F.J.M.; Tanenbaum, M.E. Translation and Replication Dynamics of Single RNA Viruses. Cell, 2020, 183(7), 1930–1945.e23. doi: 10.1016/j.cell.2020.10.019
  3. Kim, S.M.; Park, J.; Kim, M.S.; Song, H.; Jo, A.; Park, H.; Kim, T.S.; Choi, S.H.; Park, S.B. Phenotypic Discovery of an Antivirulence Agent against Vibrio vulnificus via Modulation of Quorum-Sensing Regulator SmcR. ACS Infectious Diseases, 2020, 6(11), 3076–3082. doi: 10.1021/acsinfecdis.0c00587
  4. von Döhren, J.; Kuhl, S. A simple method for long-term vital-staining of ciliated epidermal cells in aquatic larvae. Journal of Biological Methods, 2020, 7(2), e132. doi: 10.14440/jbm.2020.320
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