Cyanine5 azide

Cat. # Quantity Price Lead time
13030 100 uL, 10 mM/DMSO $110.00 in stock
33030 500 uL, 10 mM/DMSO $210.00 in stock
43030 1 mL, 10 mM/DMSO $410.00 in stock
A3030 1 mg $110.00 in stock
B3030 5 mg $210.00 in stock
C3030 10 mg $310.00 in stock
D3030 25 mg $410.00 in stock
E3030 50 mg $695.00 in stock
F3030 100 mg $1190.00 in stock

Cyanine5 azide labeling reagent for Click Chemistry, available as 10 mM solution in DMSO, and in solid form.

This azide is soluble in organic solvents (e.g. DMSO, DMF), therefore the labeling reaction should be carried out with a small amount of an organic co-solvent. This azide can be used for the labeling of alkyne-modified biomolecules in mixtures of water with organic solvents. The solution in DMSO is ready for use in bioconjugation. A water-soluble sulfonated version of this reagent is also available.

Cyanine5 is an analog of Cy5®, one of the most commonly used fluorophores which is compatible with various instruments. Cyanine5 can also be used as a replacement for Alexa Fluor® 647, and DyLight® 649.

Cyanine5 absorbance and emission spectra

Cyanine5 absorbance and emission spectra

Customers also purchased with this product

BDP FL maleimide

BDP FL maleimide is a bright and photostable thiol-reactive dye for protein labeling, an ideal replacement for fluorescein for microscopy.

Coumarin 343 X azide

Coumarin 343 is a blue emitting fluorophore that can serve as a FRET donor for fluorescein (FAM). This azide derivative can be conjugated with alkynes by Click chemistry. An extended linker arm between the fluorophore and the azide group enhances solubility, and provides spatial separation between the dye and the biomolecule.

Alkyne Phosphoramidite, 5'-terminal

Alkyne phosphoramidite for the synthesis of oligonucleotides ready for Click chemistry.

General properties

Appearance: dark blue powder / solution
Molecular weight: 601.22
CAS number: 1267539-32-1 (chloride), 1267804-34-1
Molecular formula: C35H45ClN6O
Solubility: soluble in organic solvents (DMSO, DMF, dichloromethane), very poorly soluble in water (0.63 mM, 110 mg/L)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2
CF260: 0.03
CF280: 0.04

Product citations

  1. von Döhren, J.; Kuhl, S. A simple method for long-term vital-staining of ciliated epidermal cells in aquatic larvae. Journal of Biological Methods, 2020, 7(2), e132. doi: 10.14440/jbm.2020.320
  2. Qiu, J.; Li, X.; Steenkeste, K.; Barroca-Aubry, N.; Aymes-Chodur, C.; Roger, P.; Casas-Solvas, J.M.; Vargas-Berenguel, A.; Rihouey, C.; Picton, L.; Gref, R. Self-assembled multifunctional core-shell highly porous metal-organic framework nanoparticles. International Journal of Pharmaceutics, 2020, 581, 119281. doi: 10.1016/j.ijpharm.2020.119281
  3. Tan, M.S.Y.; Davison, D.; Sanchez, M.I.; Anderson, B.M.; Howell, S.; Snijders, A.; Edgington-Mitchell, L.E.; Deu, E. Novel broad-spectrum activity-based probes to profile malarial cysteine proteases. PLoS One, 2020, 15(1), e0227341. doi: 10.1371/journal.pone.0227341
  4. He, H.; Sofman, M.; Wang, A.J.-S.; Ahrens, C.C.; Wang, W.; Griffith, L.G.; Hammond, P.T. Engineering Helical Modular Polypeptide-Based Hydrogels as Synthetic Extracellular Matrices for Cell Culture. Biomacromolecules, 2020, 21(2), 566–580. doi: 10.1021/acs.biomac.9b01297
Show more (37)
Your item has been added. View your cart or proceed to checkout
The count of items is incorrect.