Cyanine5 azide

Azides Click chemistry Cyanine5

Cat. #	Quantity	Price	Lead time
13030	100 uL, 10 mM/DMSO	–	in stock
33030	500 uL, 10 mM/DMSO	$199	in stock
43030	1 mL, 10 mM/DMSO	$495	in stock
A3030	1 mg	–	in stock
B3030	5 mg	$199	in stock
C3030	10 mg	$297	in stock
D3030	25 mg	$495	in stock
E3030	50 mg	$895	in stock
F3030	100 mg	$1490	in stock

Found better price? Let us know and we will propose the way forward!

Cyanine5 azide labeling reagent for Click Chemistry, available as 10 mM solution in DMSO and in solid form.

This azide is soluble in organic solvents (e.g., DMSO, DMF); therefore, the labeling reaction should be carried out with a small amount of an organic co-solvent. This azide can be used for the labeling of alkyne-modified biomolecules in mixtures of water with organic solvents. The solution in DMSO is ready for use in bioconjugation. A water-soluble sulfonated version of this reagent is also available.

Cyanine5 is an analog of Cy5®, one of the most commonly used fluorophores, which is compatible with various instruments. Cyanine5 can also be used as a replacement for DyLight^® 649.

Download in
ChemDraw format

Absorbance and emission spectra of Cyanine5

Recommended protocol

Conjugation of Alkyne- or Azide-modified Proteins with Dye Azides or Alkynes

Conjugation of Alkyne-modified Oligonucleotides with Dye Azides

Calculator

Solution calculator

General properties

Appearance:	dark blue powder / solution
Molecular weight:	601.22
CAS number:	1267539-32-1 (chloride)
Molecular formula:	C₃₅H₄₅ClN₆O
Solubility:	soluble in organic solvents (DMSO, DMF, dichloromethane), very poorly soluble in water (0.63 mM, 110 mg/L)
Quality control:	NMR ¹H, HPLC-MS (95%)
Storage conditions:	Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	646
ε, L⋅mol⁻¹⋅cm⁻¹:	250000
Emission maximum, nm:	662
Fluorescence quantum yield:	0.2
CF₂₆₀:	0.03
CF₂₈₀:	0.04

Product citations

Xu, M.J.; Jordan, P.W. SMC5/6 Promotes Replication Fork Stability via Negative Regulation of the COP9 Signalosome. International Journal of Molecular Sciences, 2024, 25(2), 952. doi: 10.3390/ijms25020952
Ryl, T.; Afanasyeva, E.; Hartmann, T.; Schwermer, M.; Schneider, M.; Schröder, C.; Wagemanns, M.; Bister, A.; Kanber, D.; Steenpass, L.; Schramm, K.; Jones, B.; Jones, D.T.W.; Biewald, E.; Astrahantseff, K.; Hanenberg, H.; Rahmann, S.; Lohmann, D.R.; Schramm, A.; Ketteler, P. A MYCN-driven de-differentiation profile identifies a subgroup of aggressive retinoblastoma. Communications Biology, 2024, 7, 919. doi: 10.1038/s42003-024-06596-6
Ramezani, M.; Bauman, J.; Singh, A.; Weisbart, E.; Yong, J.; Lozada, M.; Way, G.P.; Kavari, S.L.; Diaz, C.; Haghighi, M.; Batista, T.M.; Pérez-Schindler, J.; Claussnitzer, M.; Singh, S.; Cimini, B.A.; Blainey, P.C.; Carpenter, A.E.; Jan, C.H.; Neal, J.T. A genome-wide atlas of human cell morphology. bioRxiv, 2023. doi: 10.1101/2023.08.06.552164
Tsuchiya, M.; Tachibana, N.; Hamachi, I. Flow cytometric analysis of phosphatidylcholine metabolism using organelle-selective click labeling. STAR Protocols, 2023, 4(3), 102525. doi: 10.1016/j.xpro.2023.102525

Show more (50)

Cy® is a trademark of Cytiva.

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.