Cyanine5 azide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 13030 | 100 uL, 10 mM/DMSO | $110.00 | in stock | |
| 33030 | 500 uL, 10 mM/DMSO | $210.00 | in stock | |
| 43030 | 1 mL, 10 mM/DMSO |
$410.00
|
in stock | |
| A3030 | 1 mg | $110.00 | in stock | |
| B3030 | 5 mg | $210.00 | in stock | |
| C3030 | 10 mg | $310.00 | in stock | |
| D3030 | 25 mg |
$410.00
|
5 days | |
| E3030 | 50 mg |
$695.00
|
in stock | |
| F3030 | 100 mg |
$1190.00
|
in stock |
Cyanine5 azide labeling reagent for Click Chemistry, available as 10 mM solution in DMSO, and in solid form.
This azide is soluble in organic solvents (e.g. DMSO, DMF), therefore the labeling reaction should be carried out with a small amount of an organic co-solvent. This azide can be used for the labeling of alkyne-modified biomolecules in mixtures of water with organic solvents. The solution in DMSO is ready for use in bioconjugation. A water-soluble sulfonated version of this reagent is also available.
Cyanine5 is an analog of Cy5®, one of the most commonly used fluorophores which is compatible with various instruments. Cyanine5 can also be used as a replacement for Alexa Fluor® 647, and DyLight® 649.
Cyanine5 absorbance and emission spectra
Recommended protocol
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Sulfo-Cyanine5.5 NHS ester
Water soluble, sulfonated, far-red sulfo-Cyanine5.5 dye in the form of NHS ester.sulfo-Cyanine5 DBCO
Water soluble cycloalkyne for copper free Click chemistry reaction with azides. A derivative of sulfo-Cyanine5 aka Cy5.Cyanine3 maleimide
Cyanine3 maleimide is a thiol mono reactive dye.General properties
| Appearance: | dark blue powder / solution |
| Molecular weight: | 601.22 |
| CAS number: | 1267539-32-1 (chloride), 1267804-34-1 |
| Molecular formula: | C35H45ClN6O |
| Solubility: | soluble in organic solvents (DMSO, DMF, dichloromethane), very poorly soluble in water (0.63 mM, 110 mg/L) |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 250000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.2 |
| CF260: | 0.03 |
| CF280: | 0.04 |
Product citations
- He, H.; Sofman, M.; Wang, A.J.-S.; Ahrens, C.C.; Wang, W.; Griffith, L.G.; Hammond, P.T. Engineering Helical Modular Polypeptide-Based Hydrogels as Synthetic Extracellular Matrices for Cell Culture. Biomacromolecules, in press. doi: 10.1021/acs.biomac.9b01297
- Chen, F.; Bai, M.; Cao, X.; Zhao, Y.; Xue, J.; Zhao, Y. Click-encoded rolling FISH for visualizing single-cell RNA polyadenylation and structures. Nucleic Acids Research, 2019, 47(22), e145. doi: 10.1093/nar/gkz852
- Nozeret, K.; Boucharlat, A.; Agou, F.; Buddelmeijer, N. A sensitive fluorescence-based assay to monitor enzymatic activity of the essential integral membrane protein Apolipoprotein N-acyltransferase (Lnt). Scientific Reports, 2019, 9, 15978. doi: 10.1038/s41598-019-52106-8
- Harris, J.M.; Bentley, M.D.; Moreadith, R.W.; Viegas, T.X.; Fang, Z.; Yoon, K.; Weimer, R.; Dizman, B.; Nordstierna, L. Tuning Drug Release From Polyoxazoline-Drug Conjugates. European Polymer Journal, 2019, 120, 109241. doi: 10.1016/j.eurpolymj.2019.109241
Cy® is a trademark of GE Healthcare.
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