Cyanine5 azide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 13030 | 100 uL, 10 mM/DMSO | $110.00 | in stock | |
| 33030 | 500 uL, 10 mM/DMSO | $210.00 | in stock | |
| 43030 | 1 mL, 10 mM/DMSO |
$410.00
|
in stock | |
| A3030 | 1 mg | $110.00 | in stock | |
| B3030 | 5 mg | $210.00 | in stock | |
| C3030 | 10 mg | $310.00 | in stock | |
| D3030 | 25 mg |
$410.00
|
in stock | |
| E3030 | 50 mg |
$695.00
|
in stock | |
| F3030 | 100 mg |
$1190.00
|
in stock |
Cyanine5 azide labeling reagent for Click Chemistry, available as 10 mM solution in DMSO, and in solid form.
This azide is soluble in organic solvents (e.g. DMSO, DMF), therefore the labeling reaction should be carried out with a small amount of an organic co-solvent. This azide can be used for the labeling of alkyne-modified biomolecules in mixtures of water with organic solvents. The solution in DMSO is ready for use in bioconjugation. A water-soluble sulfonated version of this reagent is also available.
Cyanine5 is an analog of Cy5®, one of the most commonly used fluorophores which is compatible with various instruments. Cyanine5 can also be used as a replacement for Alexa Fluor® 647, and DyLight® 649.
Cyanine5 absorbance and emission spectra
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EdU is a nucleoside which is incorporated into replicated DNA by cellular enzymes. After it, DNA contained in the cells can be developed by Click chemistry reaction with fluorescent dye azides to reveal cell proliferation.Coumarin 343 X azide
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Ascorbic acid for the reduction of Cu(II)-TBTA complex.General properties
| Appearance: | dark blue powder / solution |
| Molecular weight: | 601.22 |
| CAS number: | 1267539-32-1 (chloride), 1267804-34-1 |
| Molecular formula: | C35H45ClN6O |
| Solubility: | soluble in organic solvents (DMSO, DMF, dichloromethane), very poorly soluble in water (0.63 mM, 110 mg/L) |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 250000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.2 |
| CF260: | 0.03 |
| CF280: | 0.04 |
Product citations
- von Döhren, J.; Kuhl, S. A simple method for long-term vital-staining of ciliated epidermal cells in aquatic larvae. Journal of Biological Methods, 2020, 7(2), e132. doi: 10.14440/jbm.2020.320
- Qiu, J.; Li, X.; Steenkeste, K.; Barroca-Aubry, N.; Aymes-Chodur, C.; Roger, P.; Casas-Solvas, J.M.; Vargas-Berenguel, A.; Rihouey, C.; Picton, L.; Gref, R. Self-assembled multifunctional core-shell highly porous metal-organic framework nanoparticles. International Journal of Pharmaceutics, 2020, 581, 119281. doi: 10.1016/j.ijpharm.2020.119281
- Tan, M.S.Y.; Davison, D.; Sanchez, M.I.; Anderson, B.M.; Howell, S.; Snijders, A.; Edgington-Mitchell, L.E.; Deu, E. Novel broad-spectrum activity-based probes to profile malarial cysteine proteases. PLoS One, 2020, 15(1), e0227341. doi: 10.1371/journal.pone.0227341
- He, H.; Sofman, M.; Wang, A.J.-S.; Ahrens, C.C.; Wang, W.; Griffith, L.G.; Hammond, P.T. Engineering Helical Modular Polypeptide-Based Hydrogels as Synthetic Extracellular Matrices for Cell Culture. Biomacromolecules, 2020, 21(2), 566–580. doi: 10.1021/acs.biomac.9b01297
Cy® is a trademark of GE Healthcare.
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