Cyanine5 azide

Cat. # Quantity Price Lead time
13030 100 uL, 10 mM/DMSO
– 
in stock
33030 500 uL, 10 mM/DMSO
$199.00
in stock
43030 1 mL, 10 mM/DMSO
$495.00
in stock
A3030 1 mg
– 
in stock
B3030 5 mg
$199.00
in stock
C3030 10 mg
$297.00
in stock
D3030 25 mg
$495.00
in stock
E3030 50 mg
$895.00
in stock
F3030 100 mg
$1490.00
in stock
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Cyanine5 azide labeling reagent for Click Chemistry, available as 10 mM solution in DMSO and in solid form.

This azide is soluble in organic solvents (e.g., DMSO, DMF); therefore, the labeling reaction should be carried out with a small amount of an organic co-solvent. This azide can be used for the labeling of alkyne-modified biomolecules in mixtures of water with organic solvents. The solution in DMSO is ready for use in bioconjugation. A water-soluble sulfonated version of this reagent is also available.

Cyanine5 is an analog of Cy5®, one of the most commonly used fluorophores, which is compatible with various instruments. Cyanine5 can also be used as a replacement for DyLight® 649.

Absorbance and emission spectra of Cyanine5

Absorbance and emission spectra of Cyanine5

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General properties

Appearance: dark blue powder / solution
Molecular weight: 601.22
CAS number: 1267539-32-1 (chloride)
Molecular formula: C35H45ClN6O
Solubility: soluble in organic solvents (DMSO, DMF, dichloromethane), very poorly soluble in water (0.63 mM, 110 mg/L)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2
CF260: 0.03
CF280: 0.04

Product citations

  1. Cedrún-Morales, M.; Migliavacca, M.; Ceballos, M.; Perez-Maseda, M.; Zampini, G.; Alameda Felgueiras, M. T.; Ostolaza-Paraiso, J.; Juanes, M.; Rincón, I.; Fairen-Jimenez, D.; Montenegro, J.; Horcajada, P.; Polo, E.; Pelaz, B.; del Pino, P. Clickable Polymer-Based Coatings for Modulating the Interaction of Metal–Organic Framework Nanocrystals with Living Cells. ACS Appl. Mater. Interfaces, 2025, 17(17), 24994–25010. doi: 10.1021/acsami.5c01695
  2. Baudat, R.; Montnach, J.; Benoit, E.; Zoukimian, C.; Carvalhosa, C.; Béroud, R.; Waard, M. D.; Servent, D. Cyanine 5-Huwentoxin-IV as a Novel Imaging Probe to Detect hNav1.7 Channel Overexpressed in Non-Small Cell Lung Cancer. Biochemical Pharmacology, 2025, 242, 117203. doi: 10.1016/j.bcp.2025.117203
  3. Lifwergren, P.; Schoen, V.; Naeimipour, S.; Khare, L.; Wunder, A.; Blom, H.; Martinez, J. G.; Pagella, P.; Fridberger, A.; Junker, J.; Aili, D. Printing and Rerouting of Elastic and Protease Responsive Shape Memory Hydrogel Filaments. Adv Healthcare Materials, 2025, 14(22), 2502262. doi: 10.1002/adhm.202502262
  4. Beranova, M.; Grossova, P.; Demuth, J.; Kostelansky, F.; Novakova, V.; Zimcik, P.; Miletin, M. Strain Promoted Click Labeling of Oligonucleotides on Solid-Phase Support. Methods, 2025, 244, 46–54. doi: 10.1016/j.ymeth.2025.09.001
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