Cyanine5 azide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 13030 | 100 uL, 10 mM/DMSO | $110.00 | in stock | |
| 33030 | 500 uL, 10 mM/DMSO | $210.00 | in stock | |
| 43030 | 1 mL, 10 mM/DMSO |
$410.00
|
in stock | |
| A3030 | 1 mg | $110.00 | in stock | |
| B3030 | 5 mg | $210.00 | in stock | |
| C3030 | 10 mg | $310.00 | in stock | |
| D3030 | 25 mg |
$410.00
|
in stock | |
| E3030 | 50 mg |
$695.00
|
in stock | |
| F3030 | 100 mg |
$1190.00
|
in stock |
Cyanine5 azide labeling reagent for Click Chemistry, available as 10 mM solution in DMSO, and in solid form.
This azide is soluble in organic solvents (e.g. DMSO, DMF), therefore the labeling reaction should be carried out with a small amount of an organic co-solvent. This azide can be used for the labeling of alkyne-modified biomolecules in mixtures of water with organic solvents. The solution in DMSO is ready for use in bioconjugation. A water-soluble sulfonated version of this reagent is also available.
Cyanine5 is an analog of Cy5®, one of the most commonly used fluorophores which is compatible with various instruments. Cyanine5 can also be used as a replacement for Alexa Fluor® 647, and DyLight® 649.
Cyanine5 absorbance and emission spectra
Recommended protocol
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General properties
| Appearance: | dark blue powder / solution |
| Molecular weight: | 601.22 |
| Molecular formula: | C35H45ClN6O |
| Solubility: | soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 250000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.2 |
| CF260: | 0.03 |
| CF280: | 0.04 |
Product citations
- Fu, Y.; Long, M.J.C.; Wisitpitthaya, S.; Inayat, H.; Pierpont, T.M.; Elsaid, I.M.; Bloom, J.C.; Ortega, J.; Weiss, R.S.; Aye, Y. Nuclear RNR-α antagonizes cell proliferation by directly inhibiting ZRANB3. Nature Chemical Biology, 2018, 14(10), 943–954. doi: 10.1038/s41589-018-0113-5
- Surya, S.L.; Long, M.J.C.; Urul, D.A.; Zhao, Y.; Mercer, E.J.; EIsaid, I.M.; Evans, T.; Aye, Y. Cardiovascular Small Heat Shock Protein HSPB7 Is a Kinetically Privileged Reactive Electrophilic Species (RES) Sensor. ACS Chemical Biology, 2018, 13(7), 1824–1831. doi: 10.1021/acschembio.7b00925
- Owiti, N.; Wei, S.; Bhagwat, A.; Kim, N. Unscheduled DNA synthesis leads to elevated uracil residues at highly transcribed genomic loci in Saccharomyces cerevisiae. PLoS Genetics, 2018, 14(7), e1007516. doi: 10.1371/journal.pgen.1007516
- Zemella, A.; Thoring, L.; Hoffmeister, C.; Šamalíková, M.; Ehren, P.; Wüstenhagen, D.A.; Kubick, S. Cell-free protein synthesis as a novel tool for directed glycoengineering of active erythropoietin. Scientific Reports, 2018, 8, 8514. doi: 10.1038/s41598-018-26936-x
Cy® is a trademark of GE Healthcare.
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