Cyanine5 azide

Cat. # Quantity Price Lead time
13030 100 uL, 10 mM/DMSO $110.00 5 days
33030 500 uL, 10 mM/DMSO $210.00 in stock
43030 1 mL, 10 mM/DMSO $410.00 in stock
A3030 1 mg $110.00 in stock
B3030 5 mg $210.00 in stock
C3030 10 mg $310.00 in stock
D3030 25 mg $410.00 in stock
E3030 50 mg $695.00 in stock
F3030 100 mg $1190.00 in stock

Cyanine5 azide labeling reagent for Click Chemistry, available as 10 mM solution in DMSO, and in solid form.

This azide is soluble in organic solvents (e.g. DMSO, DMF), therefore the labeling reaction should be carried out with a small amount of an organic co-solvent. This azide can be used for the labeling of alkyne-modified biomolecules in mixtures of water with organic solvents. The solution in DMSO is ready for use in bioconjugation. A water-soluble sulfonated version of this reagent is also available.

Cyanine5 is an analog of Cy5®, one of the most commonly used fluorophores which is compatible with various instruments. Cyanine5 can also be used as a replacement for Alexa Fluor® 647, and DyLight® 649.

Cyanine5 absorbance and emission spectra

Cyanine5 absorbance and emission spectra

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General properties

Appearance: dark blue powder / solution
Molecular weight: 601.22
CAS number: 1267539-32-1 (chloride), 1267804-34-1
Molecular formula: C35H45ClN6O
Solubility: soluble in organic solvents (DMSO, DMF, dichloromethane), very poorly soluble in water (0.63 mM, 110 mg/L)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2
CF260: 0.03
CF280: 0.04

Product citations

  1. Chen, F.; Bai, M.; Cao, X.; Zhao, Y.; Xue, J.; Zhao, Y. Click-encoded rolling FISH for visualizing single-cell RNA polyadenylation and structures. Nucleic Acids Research, in press. doi: 10.1093/nar/gkz852
  2. Harris, J.M.; Bentley, M.D.; Moreadith, R.W.; Viegas, T.X.; Fang, Z.; Yoon, K.; Weimer, R.; Dizman, B.; Nordstierna, L. Tuning Drug Release From Polyoxazoline-Drug Conjugates. European Polymer Journal, 2019, 120, 109241. doi: 10.1016/j.eurpolymj.2019.109241
  3. Rancan, F.; Volkmann, H.; Giulbudagian, M.; Schumacher, F.; Stanko, J.I.; Kleuser, B.; Blume-Peytavi, U.; Calderón, M.; Vogt, A. Dermal Delivery of the High-Molecular-Weight Drug Tacrolimus by Means of Polyglycerol-Based Nanogels. Pharmaceutics, 2019, 11(8), E394. doi: 10.3390/pharmaceutics11080394
  4. Kasprzyk, R.; Starek, B.J.; Ciechanowicz, S.; Kubacka, D.; Kowalska, J.; Jemielity, J. Fluorescent turn-on probes for the development of binding and hydrolytic activity assays for mRNA cap-recognising proteins. Chemistry, 2019, 25(27), 6728–6740. doi: 10.1002/chem.201900051
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