Cyanine5 azide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 13030 | 100 uL, 10 mM/DMSO | $110.00 | in stock | |
| 33030 | 500 uL, 10 mM/DMSO | $210.00 | in stock | |
| 43030 | 1 mL, 10 mM/DMSO |
$410.00
|
in stock | |
| A3030 | 1 mg | $110.00 | in stock | |
| B3030 | 5 mg | $210.00 | in stock | |
| C3030 | 10 mg | $310.00 | in stock | |
| D3030 | 25 mg |
$410.00
|
in stock | |
| E3030 | 50 mg |
$695.00
|
in stock | |
| F3030 | 100 mg |
$1190.00
|
in stock |
Cyanine5 azide labeling reagent for Click Chemistry, available as 10 mM solution in DMSO, and in solid form.
This azide is soluble in organic solvents (e.g. DMSO, DMF), therefore the labeling reaction should be carried out with a small amount of an organic co-solvent. This azide can be used for the labeling of alkyne-modified biomolecules in mixtures of water with organic solvents. The solution in DMSO is ready for use in bioconjugation. A water-soluble sulfonated version of this reagent is also available.
Cyanine5 is an analog of Cy5®, one of the most commonly used fluorophores which is compatible with various instruments. Cyanine5 can also be used as a replacement for Alexa Fluor® 647, and DyLight® 649.
Cyanine5 absorbance and emission spectra
Recommended protocol
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Sulfo-Cyanine3 alkyne
A water soluble dye alkyne for copper-catalyzed Click Chemistry. The fluorophore Sulfo-Cyanine3 is a bright and photostable dye for the Cy3® channel.BDP 558/568 carboxylic acid
BDP 558/568 is a borondipyrromethene dye with yellow emission. The dye as a carboxylic acid can be used in peptide synthesis (after appropriate activation of the carboxylic group).
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General properties
| Appearance: | dark blue powder / solution |
| Molecular weight: | 601.22 |
| CAS number: | 1267539-32-1 (chloride), 1267804-34-1 |
| Molecular formula: | C35H45ClN6O |
| Solubility: | soluble in organic solvents (DMSO, DMF, dichloromethane), very poorly soluble in water (0.63 mM, 110 mg/L) |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 250000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.2 |
| CF260: | 0.03 |
| CF280: | 0.04 |
Product citations
- Kasprzyk, R.; Starek, B.J.; Ciechanowicz, S.; Kubacka, D.; Kowalska, J.; Jemielity, J. Fluorescent turn-on probes for the development of binding and hydrolytic activity assays for mRNA cap-recognising proteins. Chemistry, 2019, 25(27), 6728–6740. doi: 10.1002/chem.201900051
- Lo, C.-W.; Kryvalap, Y.; Sheu, T.-J.; Chang, C.-H.; Czyzyk, J. Cellular proliferation in mouse and human pancreatic islets is regulated by serpin B13 inhibition and downstream targeting of E-cadherin by cathepsin L. Diabetologia, 2019, 62(5), 822–834. doi: 10.1007/s00125-019-4834-0
- Fu, Y.; Long, M.J.C.; Wisitpitthaya, S.; Inayat, H.; Pierpont, T.M.; Elsaid, I.M.; Bloom, J.C.; Ortega, J.; Weiss, R.S.; Aye, Y. Nuclear RNR-α antagonizes cell proliferation by directly inhibiting ZRANB3. Nature Chemical Biology, 2018, 14(10), 943–954. doi: 10.1038/s41589-018-0113-5
- Surya, S.L.; Long, M.J.C.; Urul, D.A.; Zhao, Y.; Mercer, E.J.; EIsaid, I.M.; Evans, T.; Aye, Y. Cardiovascular Small Heat Shock Protein HSPB7 Is a Kinetically Privileged Reactive Electrophilic Species (RES) Sensor. ACS Chemical Biology, 2018, 13(7), 1824–1831. doi: 10.1021/acschembio.7b00925
Cy® is a trademark of GE Healthcare.
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