LumiTracker® Mito Green FM
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3527-50ug | 50 ug |
–
|
in stock | |
| 3527-500ug | 500 ug |
$239.90
|
in stock |
LumiTracker® Mito Green FM is a cationic green-fluorescent dye that stains mitochondria in live cells. The dye passively diffuses across the plasma membrane and, unlike other mitochondrial dyes, selectively accumulates in the mitochondrial matrix in a potential-independent manner [1]. Because of this, LumiTracker Mito Green FM is more often used to determine mitochondrial mass and localization than to assess mitochondrial health (activity) [2]. Nevertheless, caution is needed when interpreting the results, as some studies indicate that the accumulation of LumiTracker Mito Green FM in mitochondria, albeit to a lesser extent, may also depend on the mitochondrial potential [3].
The fluorescence of LumiTracker Mito Green FM disappears after fixation with aldehydes, so this dye is only suitable for working with living cells.
[1] Cytotechnology. 2008. 56:145–149; [2] MethodsX. 2020. 7:100938; [3] Front. Cell. Neurosci. 2016. 10:76.
Product in action
Distribution of human T-cell leukemia cell populations, unstained (left) and stained (right) with LumiTracker Mito Green FM mitochondrial dye (3527-x)
Absorption and emission spectra of Mito Green FM
Recommended protocol
Calculator
Customers also purchased with this product
General properties
| Appearance: | orange solid |
| Molecular weight: | 671.88 |
| Molecular formula: | C34H28Cl5N3O |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 491 |
| ε, L⋅mol−1⋅cm−1: | 93500 |
| Emission maximum, nm: | 513 |
Your cart is empty
$ 
