AF647 NHS ester

Cat. # Quantity Price Lead time
16820 1 mg $110.00 5 days
26820 5 mg $340.00 5 days
46820 25 mg $1100.00 5 days
56820 50 mg $1890.00 in stock
66820 100 mg $2990.00 in stock

AF647 NHS ester is a bright, far-red fluorescent dye, used for cellular visualisation and labeling of antibodies, peptides, proteins, modified oligonucleotides, and other amine-containing molecules.

Due to the lack of significant self-quenching AF647 dye molecules can be conjugated to proteins at high molar ratios, which resulting in sensitive detection of low-abundance biomolecules potential.

AF647 NHS ester dye possesses high water-solubility, high fluorescence quantum yield, it is pH-insensitive over a wide molar range. Widely applied for imaging and flow cytometry.

Absorption and emission spectra of AF647

Absorption and emission spectra of AF647

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General properties

Appearance: dark violet solid
Molecular weight: 1056.33
Molecular formula: C39H44N3K3O16S4
IUPAC name: 2-((1E,3E)-5-((E)-3,3-dimethyl-5-sulfonato-1-(3-sulfonatopropyl)indolin-2-ylidene)penta-1,3-dien-1-yl)-3-(5-((2,5-dioxopyrrolidin-1-yl)oxy)-5-oxopentyl)-3-methyl-1-(3-sulfonatopropyl)-3H-indol-1-ium-5-sulfonate
Solubility: good in water, DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

ε, L⋅mol−1⋅cm−1: 191800
Fluorescence quantum yield: 0.15
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