AF 647 NHS ester
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 16820 | 1 mg | $140 | in stock | |
| 26820 | 5 mg |
$450
|
in stock | |
| 46820 | 25 mg |
$1460
|
in stock | |
| 56820 | 50 mg |
$1890
|
in stock | |
| 66820 | 100 mg |
$2640
|
in stock |
AF 647 NHS ester is a bright, far-red fluorescent dye, used for cellular visualization and labeling of antibodies, peptides, proteins, modified oligonucleotides, and other amine-containing molecules.
Due to the lack of significant self-quenching AF 647 dye molecules can be conjugated to proteins at high molar ratios, resulting in the sensitive detection of low-abundance biomolecules potential.
AF 647 NHS ester dye possesses high water-solubility and high fluorescence quantum yield; it is pH-insensitive over a wide molar range. Widely applied for imaging and flow cytometry.
Absorption and emission spectra of AF 647
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General properties
| Appearance: | dark violet solid |
| Molecular weight: | 1056.33 |
| Molecular formula: | C39H44N3K3O16S4 |
| IUPAC name: | 2-((1E,3E)-5-((E)-3,3-dimethyl-5-sulfonato-1-(3-sulfonatopropyl)indolin-2-ylidene)penta-1,3-dien-1-yl)-3-(5-((2,5-dioxopyrrolidin-1-yl)oxy)-5-oxopentyl)-3-methyl-1-(3-sulfonatopropyl)-3H-indol-1-ium-5-sulfonate |
| Solubility: | good in water, DMSO, DMF |
| Quality control: | NMR 1H, HPLC-MS (90%) |
| Storage conditions: | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 655 |
| ε, L⋅mol−1⋅cm−1: | 191800 |
| Emission maximum, nm: | 680 |
| Fluorescence quantum yield: | 0.15 |
| CF260: | 0.09 |
| CF280: | 0.08 |
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