AF 647 NHS ester

Cat. # Quantity Price Lead time
16820 1 mg $140 in stock
26820 5 mg $450 in stock
46820 25 mg $1460 in stock
56820 50 mg $1890 in stock
66820 100 mg $2640 in stock
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AF 647 NHS ester is a bright, far-red fluorescent dye, used for cellular visualization and labeling of antibodies, peptides, proteins, modified oligonucleotides, and other amine-containing molecules.

Due to the lack of significant self-quenching AF 647 dye molecules can be conjugated to proteins at high molar ratios, resulting in the sensitive detection of low-abundance biomolecules potential.

AF 647 NHS ester dye possesses high water-solubility and high fluorescence quantum yield; it is pH-insensitive over a wide molar range. Widely applied for imaging and flow cytometry.

Absorption and emission spectra of AF 647

Absorption and emission spectra of AF 647

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General properties

Appearance: dark violet solid
Molecular weight: 1056.33
Molecular formula: C39H44N3K3O16S4
IUPAC name: 2-((1E,3E)-5-((E)-3,3-dimethyl-5-sulfonato-1-(3-sulfonatopropyl)indolin-2-ylidene)penta-1,3-dien-1-yl)-3-(5-((2,5-dioxopyrrolidin-1-yl)oxy)-5-oxopentyl)-3-methyl-1-(3-sulfonatopropyl)-3H-indol-1-ium-5-sulfonate
Solubility: good in water, DMSO, DMF
Quality control: NMR 1H, HPLC-MS (90%)
Storage conditions: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 655
ε, L⋅mol−1⋅cm−1: 191800
Emission maximum, nm: 680
Fluorescence quantum yield: 0.15
CF260: 0.09
CF280: 0.08
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