AF 647 NHS ester
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 16820 | 1 mg |
$140
|
in stock | |
| 26820 | 5 mg |
$450
|
in stock | |
| 46820 | 25 mg |
$1460
|
in stock | |
| 56820 | 50 mg |
$1890
|
in stock | |
| 66820 | 100 mg |
$2640
|
in stock |
AF 647 NHS ester is a bright, far-red fluorescent dye, used for cellular visualization and labeling of antibodies, peptides, proteins, modified oligonucleotides, and other amine-containing molecules.
Due to the lack of significant self-quenching AF 647 dye molecules can be conjugated to proteins at high molar ratios, resulting in the sensitive detection of low-abundance biomolecules potential.
AF 647 NHS ester dye possesses high water-solubility and high fluorescence quantum yield; it is pH-insensitive over a wide molar range. Widely applied for imaging and flow cytometry.
Absorption and emission spectra of AF 647
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Activated ester for modification of proteins/antibodies/peptides followed by introduction into copper-free click chemistry reaction.General properties
| Appearance: | dark violet solid |
| Molecular weight: | 1056.33 |
| Molecular formula: | C39H44N3K3O16S4 |
| IUPAC name: | 2-((1E,3E)-5-((E)-3,3-dimethyl-5-sulfonato-1-(3-sulfonatopropyl)indolin-2-ylidene)penta-1,3-dien-1-yl)-3-(5-((2,5-dioxopyrrolidin-1-yl)oxy)-5-oxopentyl)-3-methyl-1-(3-sulfonatopropyl)-3H-indol-1-ium-5-sulfonate |
| Solubility: | good in water, DMSO |
| Quality control: | NMR 1H, HPLC-MS (90%) |
| Storage conditions: | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 655 |
| ε, L⋅mol−1⋅cm−1: | 191800 |
| Emission maximum, nm: | 680 |
| Fluorescence quantum yield: | 0.15 |
| CF260: | 0.09 |
| CF280: | 0.08 |
Product citations
- Afting, C.; Walther, T.; Drozdowski, O. M.; Schlagheck, C.; Schwarz, U. S.; Wittbrodt, J.; Göpfrich, K. DNA Microbeads for Spatio-Temporally Controlled Morphogen Release within Organoids. Nat. Nanotechnol., 2024, 1-9. doi: 10.1038/s41565-024-01779-y
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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