Activated esters
Primary or secondary amino groups are ubiquitous in biomolecules. They are present in proteins and peptides as ε-amino groups of lysines and N-terminal amino groups. Many natural and synthetic small molecules are amines, and DNAs with amino groups can be prepared by solid phase synthesis, and enzymatically.
Amino groups can be modified by acylation with various activated carbonyl compounds. Among these compounds, activated N-hydroxysuccinimide esters are most suitable for biolabeling and bioconjugation applications.
Compared to other activated esters, NHS derivatives possess good stability in aqueous environment. Optimal pH for the coupling of amines with NHS esters in aqueous buffers is 8.3-8.5. Labeling is also possible in organic solvents. Polar aprotic solents, such as DMF or DMSO, are preferred. One equivalent of tertiary amine base, such as triethylamine, or diisopropylethylamine (DIPEA) is necessary to achieve efficient amine acylation in non-aqueous solution.
(5,6)-FAM diacetate NHS ester
Amine-reactive fluorescein (FAM) derivative, a mixture of 5- and 6-isomers. The reagent becomes brightly green fluorescent once it is hydrolyzed while diffusing into cells. A fluorescent tracer.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2934-1mg | 1 mg | – | in stock | |
| 2934-25mg | 25 mg | $160 | in stock | |
| 2934-50mg | 50 mg | $270 | in stock | |
| 2934-100mg | 100 mg |
$520
|
in stock |
AF 343 (Coumarin)-X-NHS ester
AF 343 (Coumarin) is a blue emitting dye that can serve as a FRET donor for fluorescein (FAM). This structure features a linker between Coumarin 343 fluorophore, and the functional group. It provides better solubility, and spatial separation between the dye, and the functional group.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 12620 | 1 mg | $110 | in stock | |
| 22620 | 5 mg | $210 | in stock | |
| 42620 | 25 mg |
$410
|
in stock | |
| 52620 | 50 mg |
$695
|
in stock | |
| 62620 | 100 mg |
$1190
|
in stock |
AF 430 NHS ester
AF 430 is a hydrophilic coumarin dye with emission in the green-yellow range. The NHS ester is used for the labeling of amine groups.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 10820 | 1 mg | $110 | in stock | |
| 20820 | 5 mg | $165 | in stock | |
| 40820 | 25 mg | $380 | in stock | |
| 50820 | 50 mg |
$695
|
in stock | |
| 60820 | 100 mg |
$1190
|
in stock |
AF 488 NHS ester
AF 488 NHS ester, a bright, photostable, and hydrophilic fluorescent dye for the green channel.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 11820 | 1 mg | $125 | in stock | |
| 21820 | 5 mg | $325 | in stock | |
| 31820 | 10 mg |
$480
|
in stock | |
| 41820 | 25 mg |
$950
|
5 days | |
| 51820 | 50 mg |
$1625
|
in stock | |
| 61820 | 100 mg |
$2090
|
in stock |
AF 488 TFP ester
Tetrafluorophenyl ester of green fluorescent dye AF 488 for labeling amino groups in proteins, peptides, and modified oligonucleotides.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 118T0 | 1 mg | $165 | in stock | |
| 218T0 | 5 mg |
$510
|
in stock | |
| 418T0 | 25 mg |
$1650
|
in stock |
AF 532 NHS ester
NHS-ester of AF 532 dye for protein, peptide, and modified oligonucleotide labeling via reaction with amino groups.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1C820 | 1 mg | $140 | in stock | |
| 2C820 | 5 mg |
$450
|
in stock | |
| 4C820 | 25 mg |
$1460
|
in stock | |
| 5C820 | 50 mg |
$1890
|
in stock | |
| 6C820 | 100 mg |
$2640
|
in stock |
AF 555 NHS ester
AF 555 NHS ester, a bright, photostable, and hydrophilic fluorescent dye for the yellow channel.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1K820 | 1 mg | $140 | in stock | |
| 2K820 | 5 mg |
$450
|
in stock | |
| 4K820 | 25 mg |
$1460
|
in stock | |
| 5K820 | 50 mg |
$1890
|
in stock | |
| 6K820 | 100 mg |
$2640
|
in stock |
AF 568 NHS ester
AF 568 derivative for labeling proteins and other high- and low-molecular weight ligands with free amines.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 14820 | 1 mg | $125 | in stock | |
| 24820 | 5 mg | $325 | in stock | |
| 44820 | 25 mg |
$720
|
in stock | |
| 54820 | 50 mg |
$1325
|
in stock | |
| 64820 | 100 mg |
$2090
|
in stock |
AF 594 NHS ester
Red-fluorescent NHS-ester of AF 594 dye for labeling proteins, peptides, antibodies, and any molecules containing an -NH2 group. Pure 5-isomer
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2825-1mg | 1 mg | $125 | in stock | |
| 2825-5mg | 5 mg |
$425
|
in stock | |
| 2825-25mg | 25 mg |
$1240
|
in stock | |
| 2825-50mg | 50 mg |
$1890
|
in stock | |
| 2825-100mg | 100 mg |
$2640
|
in stock |
AF 647 NHS ester
Bright, water-soluble succinimide ester of AF 647 dye for protein and antibody labeling.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 16820 | 1 mg | $140 | in stock | |
| 26820 | 5 mg |
$450
|
in stock | |
| 46820 | 25 mg |
$1460
|
in stock | |
| 56820 | 50 mg |
$1890
|
in stock | |
| 66820 | 100 mg |
$2640
|
in stock |
AMCA NHS ester
NHS-ester of AMCA dye for protein, peptide, and modified oligonucleotide labeling via reaction with amino groups. AMCA is one of the brightest blue fluorescent dyes with large Stoke's shift and high resistance to photobleaching.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3606-5mg | 5 mg | $110 | in stock | |
| 3606-25mg | 25 mg | $290 | in stock | |
| 3606-50mg | 50 mg |
$435
|
in stock | |
| 3606-100mg | 100 mg |
$595
|
in stock |
ATT 550 NHS ester
Water-soluble ATT 550 succinimidyl ester for labeling various amine-containing molecules in the aqueous phase.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1C920 | 1 mg | $110 | 5 days | |
| 2C920 | 5 mg |
$640
|
5 days | |
| 4C920 | 25 mg |
$1640
|
5 days | |
| 5C920 | 50 mg |
$2090
|
5 days | |
| 6C920 | 100 mg |
$2840
|
5 days |
BDP® 558/568 NHS ester
BDP 558/568 is a fluorophore for the yellow region of the spectrum. Like other BDP dyes, this fluorophore has high fluorescence quantum yield, brightness, and photostability.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 17420 | 1 mg | $125 | in stock | |
| 27420 | 5 mg | $260 | in stock | |
| 47420 | 25 mg |
$510
|
in stock | |
| 57420 | 50 mg |
$895
|
in stock | |
| 67420 | 100 mg |
$1490
|
in stock |
BDP 564/570 NHS ester
Lypohilic borodipyrromethene dye with orange emission for the labeling of primary and secondary amine groups.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1H420 | 1 mg | $125 | in stock | |
| 2H420 | 5 mg | $325 | in stock | |
| 4H420 | 25 mg |
$510
|
in stock | |
| 5H420 | 50 mg |
$895
|
in stock | |
| 6H420 | 100 mg |
$1490
|
in stock |
BDP® 576/589 NHS ester
BDP 576/589 NHS ester is a bright hydrophobic dye with emission in the orange spectrum range. It can be used in fluorescence anisotropy assays.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1G420 | 1 mg | $125 | in stock | |
| 2G420 | 5 mg | $260 | in stock | |
| 4G420 | 25 mg |
$510
|
in stock | |
| 5G420 | 50 mg |
$895
|
in stock | |
| 6G420 | 100 mg |
$1490
|
in stock |
BDP® 581/591 NHS ester
NHS ester derivative of BDP 581/591 borondipyrromethene fluorophore for the labeling of proteins, peptides, and other biomolecules. The dye is useful for fluorescence polarization and two-photon experiments, and as a ROS probe.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 16420 | 1 mg | $125 | in stock | |
| 26420 | 5 mg | $260 | in stock | |
| 46420 | 25 mg |
$510
|
in stock | |
| 56420 | 50 mg |
$895
|
in stock | |
| 66420 | 100 mg |
$1490
|
in stock |
BDP® 630/650-X-NHS ester
BDP 630/650 is a borondipyrromethene fluorophore tuned to Cyanine5 excitation and emission channels. The dye is bright and highly photostable. It is useful for fluorescence polarization assays. This is an amine-reactive NHS ester derivative.
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| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 15420 | 1 mg | $125 | in stock | |
| 25420 | 5 mg | $260 | in stock | |
| 45420 | 25 mg |
$510
|
in stock | |
| 55420 | 50 mg |
$895
|
in stock | |
| 65420 | 100 mg |
$1490
|
in stock |