AF594 NHS ester, 5-isomer

Cat. # Quantity Price Lead time
2825-1mg 1 mg –   in stock
2825-5mg 5 mg $355.00 in stock
2825-25mg 25 mg $1150.00 in stock
2825-50mg 50 mg $1955.00 in stock
2825-100mg 100 mg $2425.00 in stock

AF594 is a bright water-soluble dye that is not sensitive to pH changes within the range from 4 to 10. This red-fluorescent dye is commonly used for flow cytometry and fluorescent microscopy.

AF594 NHS ester is used for labeling proteins, peptides, antibodies, and any molecules containing an -NH2 group (such as amino-modified oligonucleotides); it results in the formation of stable amide bonds between the dye and the target molecule. The best result in conjugation reaction achieved at pH from 7 to 9.

AF594 can be used for protein labeling with a high molar dye-to-protein ratio. The resulting conjugates with a high degree of labeling (DOL) do not exhibit significant fluorescence quenching. In contrast, the conjugates have brighter fluorescence, which allows increasing the lowest limit of detection of the labeled product.

Customers also purchased with this product

TAMRA NHS ester, 5-isomer

Amine-reactive, activated N-hydroxysuccinimidyl ester of TAMRA dye for the labeling of amine groups of proteins, peptides, and amino-oligonucleotides.

Sulfo-Cyanine5 NHS ester

Water-soluble Cyanine 5 succinimide ester.

Amino-11-dUTP

Amino-11-dUTP is a triphosphate for the modification of DNA.

General properties

Appearance: Dark-blue crystals
Molecular weight: 1022.23
CAS number: 1638544-48-5
Molecular formula: C51H67N5O13S2
IUPAC name: 5-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)-2-(1,2,2,10,10,11-hexamethyl-4,8-bis(sulfonatomethyl)-10,11-dihydro-2H-pyrano[3,2-g:5,6-g']diquinolin-1-ium-6-yl)benzoate
Solubility: soluble in water, DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 590
ε, L⋅mol−1⋅cm−1: 110000
Emission maximum, nm: 617
Fluorescence quantum yield: 0.99
CF260: 0.42
CF280: 0.60
Your item has been added. View your cart or proceed to checkout
The count of items is incorrect.