AF 594 NHS ester
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2825-1mg | 1 mg |
$125
|
in stock | |
| 2825-5mg | 5 mg |
$425
|
in stock | |
| 2825-25mg | 25 mg |
$1240
|
in stock | |
| 2825-50mg | 50 mg |
$1890
|
in stock | |
| 2825-100mg | 100 mg |
$2640
|
in stock |
AF 594 is a bright water-soluble dye that is not sensitive to pH changes within the range from 4 to 10. This red-fluorescent dye is commonly used for flow cytometry and fluorescent microscopy.
AF 594 NHS ester is used for labeling proteins, peptides, antibodies, and any molecules containing an -NH2 group (such as amino-modified oligonucleotides); it results in the formation of stable amide bonds between the dye and the target molecule. The best result in conjugation reaction achieved at pH from 7 to 9.
AF 594 can be used for protein labeling with a high molar dye-to-protein ratio. The resulting conjugates with a high degree of labeling (DOL) do not exhibit significant fluorescence quenching. In contrast, the conjugates have brighter fluorescence, which allows increasing the lowest limit of detection of the labeled product.
AF 594 absorbance and emission spectra
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A tyramide derivative of sulfo-Cyanine3, an orange fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, and FISH.General properties
| Appearance: | dark-blue crystals |
| Molecular weight: | 1022.23 |
| CAS number: | 1638544-48-5 |
| Molecular formula: | C51H67N5O13S2 |
| IUPAC name: | 5-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)-2-(1,2,2,10,10,11-hexamethyl-4,8-bis(sulfonatomethyl)-10,11-dihydro-2H-pyrano[3,2-g:5,6-g']diquinolin-1-ium-6-yl)benzoate |
| Solubility: | soluble in water, DMSO, DMF |
| Quality control: | NMR 1H (95%), HPLC-MS (80%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 586 |
| ε, L⋅mol−1⋅cm−1: | 105000 |
| Emission maximum, nm: | 613 |
| Fluorescence quantum yield: | 0.77 |
| CF260: | 0.28 |
| CF280: | 0.51 |
Product citations
- Kislova, A. V.; Zheglo, D.; Pozhitnova, V. O.; Sviridov, P. S.; Gadzhieva, E. P.; Voronina, E. S. Replication Stress Causes Delayed Mitotic Entry and Chromosome 12 Fragility at the ANKS1B Large Neuronal Gene in Human Induced Pluripotent Stem Cells. Chromosome Research, 2023, 31(3), 23. doi: 10.1007/s10577-023-09729-5
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