AF594 NHS ester, 5-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2825-1mg | 1 mg | – | in stock | |
| 2825-5mg | 5 mg | $355.00 | in stock | |
| 2825-25mg | 25 mg |
$1150.00
|
in stock | |
| 2825-50mg | 50 mg |
$1955.00
|
in stock | |
| 2825-100mg | 100 mg |
$2425.00
|
in stock |
AF594 is a bright water-soluble dye that is not sensitive to pH changes within the range from 4 to 10. This red-fluorescent dye is commonly used for flow cytometry and fluorescent microscopy.
AF594 NHS ester is used for labeling proteins, peptides, antibodies, and any molecules containing an -NH2 group (such as amino-modified oligonucleotides); it results in the formation of stable amide bonds between the dye and the target molecule. The best result in conjugation reaction achieved at pH from 7 to 9.
AF594 can be used for protein labeling with a high molar dye-to-protein ratio. The resulting conjugates with a high degree of labeling (DOL) do not exhibit significant fluorescence quenching. In contrast, the conjugates have brighter fluorescence, which allows increasing the lowest limit of detection of the labeled product.
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Amine-reactive, activated N-hydroxysuccinimidyl ester of TAMRA dye for the labeling of amine groups of proteins, peptides, and amino-oligonucleotides.Sulfo-Cyanine5 NHS ester
Water-soluble Cyanine 5 succinimide ester.Amino-11-dUTP
Amino-11-dUTP is a triphosphate for the modification of DNA.General properties
| Appearance: | Dark-blue crystals |
| Molecular weight: | 1022.23 |
| CAS number: | 1638544-48-5 |
| Molecular formula: | C51H67N5O13S2 |
| IUPAC name: | 5-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)-2-(1,2,2,10,10,11-hexamethyl-4,8-bis(sulfonatomethyl)-10,11-dihydro-2H-pyrano[3,2-g:5,6-g']diquinolin-1-ium-6-yl)benzoate |
| Solubility: | soluble in water, DMSO, DMF |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 590 |
| ε, L⋅mol−1⋅cm−1: | 110000 |
| Emission maximum, nm: | 617 |
| Fluorescence quantum yield: | 0.99 |
| CF260: | 0.42 |
| CF280: | 0.60 |
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