AF594 NHS ester, 5-isomer

Cat. # Quantity Price Lead time
2825-1mg 1 mg –   in stock
2825-5mg 5 mg $355.00 in stock
2825-25mg 25 mg $1150.00 in stock
2825-50mg 50 mg $1955.00 in stock
2825-100mg 100 mg $2425.00 in stock

AF594 is a bright water-soluble dye that is not sensitive to pH changes within the range from 4 to 10. This red-fluorescent dye is commonly used for flow cytometry and fluorescent microscopy.

AF594 NHS ester is used for labeling proteins, peptides, antibodies, and any molecules containing an -NH2 group (such as amino-modified oligonucleotides); it results in the formation of stable amide bonds between the dye and the target molecule. The best result in conjugation reaction achieved at pH from 7 to 9.

AF594 can be used for protein labeling with a high molar dye-to-protein ratio. The resulting conjugates with a high degree of labeling (DOL) do not exhibit significant fluorescence quenching. In contrast, the conjugates have brighter fluorescence, which allows increasing the lowest limit of detection of the labeled product.

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General properties

Appearance: Dark-blue crystals
Molecular weight: 1022.23
CAS number: 1638544-48-5
Molecular formula: C51H67N5O13S2
IUPAC name: 5-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)-2-(1,2,2,10,10,11-hexamethyl-4,8-bis(sulfonatomethyl)-10,11-dihydro-2H-pyrano[3,2-g:5,6-g']diquinolin-1-ium-6-yl)benzoate
Solubility: soluble in water, DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 590
ε, L⋅mol−1⋅cm−1: 110000
Emission maximum, nm: 617
Fluorescence quantum yield: 0.99
CF260: 0.42
CF280: 0.60
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