AF 594 NHS ester

NHS esters AF 594 Protein labeling

Cat. #	Quantity	Price	Lead time
2825-1mg	1 mg	$125	in stock
2825-5mg	5 mg	$425	in stock
2825-25mg	25 mg	$1240	in stock
2825-50mg	50 mg	$1890	in stock
2825-100mg	100 mg	$2640	in stock

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AF 594 is a bright water-soluble dye that is not sensitive to pH changes within the range from 4 to 10. This red-fluorescent dye is commonly used for flow cytometry and fluorescent microscopy.

AF 594 NHS ester is used for labeling proteins, peptides, antibodies, and any molecules containing an -NH2 group (such as amino-modified oligonucleotides); it results in the formation of stable amide bonds between the dye and the target molecule. The best result in conjugation reaction achieved at pH from 7 to 9.

AF 594 can be used for protein labeling with a high molar dye-to-protein ratio. The resulting conjugates with a high degree of labeling (DOL) do not exhibit significant fluorescence quenching. In contrast, the conjugates have brighter fluorescence, which allows increasing the lowest limit of detection of the labeled product.

Structure of AF 594 activated ester, 5-isomer

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AF 594 absorbance and emission spectra

General properties

Appearance:	dark-blue crystals
Molecular weight:	1022.23
CAS number:	1638544-48-5
Molecular formula:	C₅₁H₆₇N₅O₁₃S₂
IUPAC name:	5-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)-2-(1,2,2,10,10,11-hexamethyl-4,8-bis(sulfonatomethyl)-10,11-dihydro-2H-pyrano[3,2-g:5,6-g']diquinolin-1-ium-6-yl)benzoate
Solubility:	soluble in water, DMSO, DMF
Quality control:	NMR ¹H (95%), HPLC-MS (80%)
Storage conditions:	Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	586
ε, L⋅mol⁻¹⋅cm⁻¹:	105000
Emission maximum, nm:	613
Fluorescence quantum yield:	0.77
CF₂₆₀:	0.28
CF₂₈₀:	0.51

Product citations

Kislova, A. V.; Zheglo, D.; Pozhitnova, V. O.; Sviridov, P. S.; Gadzhieva, E. P.; Voronina, E. S. Replication Stress Causes Delayed Mitotic Entry and Chromosome 12 Fragility at the ANKS1B Large Neuronal Gene in Human Induced Pluripotent Stem Cells. Chromosome Research, 2023, 31(3), 23. doi: 10.1007/s10577-023-09729-5

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.