DMSO is a polar aprotic solvent widely used in bioconjugation. This solvent can be considered as very important co-solvent for Click reactions to label various objects with poorly water-soluble fluorescent dyes. DMSO is also recommended as an auxiliary solvent in the reaction mixture when a hydrophobic label is used for protein labeling, or when a label molecule needs to be aliquoted before the reaction.
Coumarin 343 is a blue emitting fluorophore that can serve as a FRET donor for fluorescein (FAM). This azide derivative can be conjugated with alkynes by Click chemistry. An extended linker arm between the fluorophore and the azide group enhances solubility, and provides spatial separation between the dye and the biomolecule.
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PEG4 amine azide (CAS# 134179-38-7) is a bifunctional linker with amine and azide functional groups. It is based on a tetraethyleneglycol core. The length of the PEG4 linker is 1.4 nm.
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Water-soluble sulfo-Cyanine3 dye azide for Click Chemistry.
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General properties
Appearance:
colorless liquid
Molecular weight:
78.14
CAS number:
67-68-5
Molecular formula:
C2H6OS
IUPAC name:
dimethylsulfoxide
Solubility:
miscible with water, many organic solvents
Quality control:
GCMS (98+%)
Storage conditions:
Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
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