Biotin-X-NHS ester

NHS esters Biotinylation Crosslinking

Cat. #	Quantity	Price	Lead time
2551-50mg	50 mg	–	in stock
2551-100mg	100 mg	$117	in stock
2551-1g	1 g	$450	in stock

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Biotin may be conjugated to many proteins while maintaining the biological activity. The biotinylated probe is usually detected by avidin, streptavidin due to their effective binding which is used in various applications such as affinity chromatography, ELISA and Western Blot, fluorescence-activated cell sorting (FACS), intracellular labeling.

Biotin-X-NHS ester is a derivative with C6-spacer to reduce steric effect when binding amino acids, peptides, or proteins by reacting with primary amines. This compound is used to attach biotin to primary amines under alkaline conditions (pH 8-9).

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General properties

Appearance:	white solid
Mass spec M+ increment:	339.2
Molecular weight:	454.55
CAS number:	72040-63-2
Molecular formula:	C₂₀H₃₀N₄O₆S
IUPAC name:	(2,5-dioxopyrrolidin-1-yl) 6-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]hexanoate
Solubility:	good in DMSO, moderately soluble in DMF
Quality control:	NMR ¹H
Storage conditions:	12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS:	Download
	Product specifications

Product citations

Rak, A.; Matyushenko, V.; Prokopenko, P.; Kostromitina, A.; Polyakov, D.; Sokolov, A.; Rudenko, L.; Isakova-Sivak, I. A novel immunofluorescent test system for SARS-CoV-2 detection in infected cells. PLOS ONE, 2024, 19(5), e0304534. doi: 10.1371/journal.pone.0304534

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.