ProteOrange Protein Gel Stain, 5000×
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 40210 | 1 mL | $195.00 | in stock |
ProteOrange is a protein-binding dye for staining of protein gels, an analog of SYPRO Orange. The fluorophore is a styrene-like zwitterionic dye. It binds proteins selectively in the presence of nucleic acids, polysaccharides, and other molecules. It is also tolerant to SDS, and therefore can be used for SDS-PAGE gel staining. The dye interacts with the SDS coat around proteins in the gel, so it gives more consistent staining between different types of proteins compared to CBB staining, and never exhibits negative staining. It also stains glycoproteins well.
Staining with ProteOrange is about an order of magnitude more sensitive than Coumassie dye staining, and allows to detect about 3 ng of protein per band (30 ng is detection limit for Coumassie).
ProteOrange is supplied as a 5000x solution in DMSO. 1 mL of the solution is enough for the staining of around 200 minigels.
Excitation and emission spectra of ProteOrange complex with protein and SDS
Recommended protocol
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Cyanine2 NHS ester minimal dye for protein labelingPico488 dsDNA quantification reagent, 200x solution in DMSO
Pico488 is a dye for precise fluorescence based quantification of dsDNA in the presence of RNA, ssDNA, and other impurities. The dye exhibits bright fluorescence in the fluorescein channel, which provides a linear response to DNA concentration within the range of low pg/mL to high ng/mL.General properties
| Appearance: | orange solution |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 470 |
| Emission maximum, nm: | 570 |
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