sulfo-Cyanine5 maleimide

Cat. # Quantity Price Lead time
13380 1 mg $115 in stock
23380 5 mg $290 in stock
43380 25 mg $695 in stock
53380 50 mg $1270 in stock
63380 100 mg $1990 in stock
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A water soluble, hydrophilic sulfo-Cyanine5 maleimide (Cy5® maleimide analog). We recommend this product for protein labeling, including labeling of antibodies as a perfect replacement for Cy5® maleimide. Labeled proteins can be easily separated from unreacted dye by gel filtration, spin column purification, dialysis, electrophoresis or chromatography.

An analog without sulfo groups, Cyanine5 maleimide, is also available.

Sulfo-Cyanine5 absorbance and emission spectra

Sulfo-Cyanine5 absorbance and emission spectra

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General properties

Appearance: dark blue powder
Molecular weight: 803.00
CAS number: 2242791-82-6
Molecular formula: C38H43KN4O9S2
Solubility: soluble in water, DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95+%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 271000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.28
CF260: 0.04
CF280: 0.04

Product citations

  1. Zhang, Y.; Liang, L.; Li, H.; Cao, Y.; Meng, D.; Li, X.; Wang, M.; Wang, J.; Yao, Y.; Zhang, S.; Chen, C.; Hou, P.; Yang, Q. Smart multi‐functional aggregates reoxygenate tumor microenvironment through a two‐pronged strategy to revitalize cancer immunotherapy. Aggregate, 2024. doi: 10.1002/agt2.545
  2. Damm, A.; Paik, S.-J.; Sadhu, R.K.; Di-Cicco, A.; Manzi, J.; Castellana, M.; Margeat, E.; Dahan, M.; Sens, P.; Lévy, D.; Bassereau, P. Mechanosensitivity of an ABC Membrane Transporter Revealed by Single Molecule FRET and Activity Measurement. bioRxiv, 2024, preprint. doi: 10.1101/2024.01.24.577054
  3. Valentino, I.M.; Llivicota-Guaman, J.G.; Dao, T.P.; Mulvey, E.O.; Lehman, A.M.; Galagedera, S.K.K.; Mallon, E.L.; Castañeda, C.A.; Kraut, D.A. Phase separation of polyubiquitinated proteins in UBQLN2 condensates controls substrate fate. bioRxiv, 2024, preprint. doi: 10.1101/2024.03.15.585243
  4. Moochickal Assainar, B.; Ragunathan, K.; Baldridge, R.D. Direct observation of autoubiquitination for an integral membrane ubiquitin ligase in ERAD. Nature Communications, 2024, 15, 1340. doi: 10.1038/s41467-024-45541-3
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