Sulfo-Cyanine5 maleimide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 13380 | 1 mg | $110.00 | in stock | |
| 23380 | 5 mg | $290.00 | in stock | |
| 43380 | 25 mg |
$690.00
|
in stock | |
| 53380 | 50 mg |
$1270.00
|
in stock | |
| 63380 | 100 mg |
$1990.00
|
in stock |
A water soluble, hydrophilic sulfo-Cyanine5 maleimide (Cy5® maleimide analog). We recommend this product for protein labeling, including labeling of antibodies as a perfect replacement for Cy5® maleimide. Labeled proteins can be easily separated from unreacted dye by gel filtration, spin column purification, dialysis, electrophoresis or chromatography.
An analog without sulfo groups, Cyanine5 maleimide, is also available.
Sulfo-Cyanine5 absorbance and emission spectra
Recommended protocol
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Water soluble cyanine dye with bright fluorescence, and high extinction coefficient. Contains an amino group for the conjugation with electrophiles, and enzymatic transamination labeling.Sulfo-Cyanine3 carboxylic acid
Sulfo-Cyanine3 free unactivated monofunctional carboxylic acid, water-soluble dye.General properties
| Appearance: | dark blue powder |
| Molecular weight: | 803.00 |
| CAS number: | 2242791-82-6 (potassium salt); 2130955-10-9 (inner salt); 2242791-81-5 (inner salt); 2301999-22-2 (sodium salt) |
| Molecular formula: | C38H43KN4O9S2 |
| Solubility: | soluble in water, DMSO, DMF |
| Quality control: | NMR 1H, HPLC-MS (95+%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 271000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.28 |
| CF260: | 0.04 |
| CF280: | 0.04 |
Product citations
- Ji, W.; Smith, P.N.; Koepsel, R.R.; Andersen, J.D.; Baker, S.L.; Zhang, L.; Carmali, S.; Myerson, J.W.; Muzykantov, V.; Russell, A.J. Erythrocytes as Carriers of Immunoglobulin-Based Therapeutics. Acta Biomaterialia, in press. doi: 10.1016/j.actbio.2019.10.027
- Baranova, N.; Radler, P.; Hernandez-Rocamora, V.M.; Alfonso, C.; Lopez-Pelegrin, M.; Rivas, G.; Vollmer, W.; Loose, M. FtsZ assembles the bacterial cell division machinery by a diffusion-and-capture mechanism. bioRxiv, preprint. doi: 10.1101/485656
- Cliff, L.; Chadda, R.; Robertson, J.L. Occupancy distributions of membrane proteins in heterogeneous liposome populations. BBA Biomembranes, 2020, 1862(1), 183033. doi: 10.1016/j.bbamem.2019.183033
- Kholodenko, I.V.; Kalinovsky, D.V.; Svirshchevskaya, E.V.; Doronin, I.I.; Konovalova, M.V.; Kibardin, A.V.; Shamanskaya, T.V.; Larin, S.S.; Deyev, S.M.; Kholodenko, R.V. Multimerization through Pegylation Improves Pharmacokinetic Properties of scFv Fragments of GD2-Specific Antibodies. Molecules, 2019, 24(21), 3835. doi: 10.3390/molecules24213835
Cy® is a trademark of GE Healthcare.
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