Sulfo-Cyanine5 maleimide

Cat. # Quantity Price Lead time
13380 1 mg $110.00 in stock
23380 5 mg $290.00 in stock
43380 25 mg $690.00 in stock
53380 50 mg $1270.00 in stock
63380 100 mg $1990.00 in stock

A water soluble, hydrophilic sulfo-Cyanine5 maleimide (Cy5® maleimide analog). We recommend this product for protein labeling, including labeling of antibodies as a perfect replacement for Cy5® maleimide. Labeled proteins can be easily separated from unreacted dye by gel filtration, spin column purification, dialysis, electrophoresis or chromatography.

An analog without sulfo groups, Cyanine5 maleimide, is also available.

Sulfo-Cyanine5 absorbance and emission spectra

Sulfo-Cyanine5 absorbance and emission spectra

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General properties

Appearance: dark blue powder
Molecular weight: 803.00
CAS number: 2242791-82-6
Molecular formula: C38H43KN4O9S2
Solubility: soluble in water, DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95+%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 271000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.28
CF260: 0.04
CF280: 0.04

Product citations

  1. Deckard, C.E.; Sczepanski, J.T. Reversible chromatin condensation by the DNA repair and demethylation factor thymine DNA glycosylase. Nucleic Acids Research, in press. doi: 10.1093/nar/gkab040
  2. Delvaux, N.A.; Mathew, B.; Rice, K.G. Fluorescent Labeling of Plasmid DNA for Gene Delivery: Implications of Dye Hydrophobicity on Labeling Efficiencies and Nanoparticle Size. Analytical Biochemistry, in press. doi: 10.1016/j.ab.2020.113895
  3. Lapenta, F.; Aupič, J.; Vezzoli, M.; Strmšek, Ž.; Da Vela, S.; Svergun, D.I.; Carazo, J.M.; Melero, R.; Jerala, R. Self-assembly and regulation of protein cages from pre-organised coiled-coil modules. Nature Communications, 2021, 12, 939. doi: 10.1038/s41467-021-21184-6
  4. Aupič, J.; Strmšek, Ž.; Lapenta, F.; Pahovnik, D.; Pisanski, T.; Drobnak, I.; Ljubetič, A.; Jerala, R. Designed folding pathway of modular coiled-coil-based proteins. Nature Communications, 2021, 12, 940. doi: 10.1038/s41467-021-21185-5
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