Sulfo-Cyanine5 maleimide

Cat. # Quantity Price Lead time
13380 1 mg $110.00 in stock
23380 5 mg $290.00 in stock
43380 25 mg $690.00 in stock
53380 50 mg $1270.00 in stock
63380 100 mg $1990.00 in stock

A water soluble, hydrophilic sulfo-Cyanine5 maleimide (Cy5® maleimide analog). We recommend this product for protein labeling, including labeling of antibodies as a perfect replacement for Cy5® maleimide. Labeled proteins can be easily separated from unreacted dye by gel filtration, spin column purification, dialysis, electrophoresis or chromatography.

An analog without sulfo groups, Cyanine5 maleimide, is also available.

Sulfo-Cyanine5 absorbance and emission spectra

Sulfo-Cyanine5 absorbance and emission spectra

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Cyanine5.5 carboxylic acid

Cy5.5 unactivated free acid cyanine dye.

Copper(II)-TBTA complex, 10 mM in 55% aq. DMSO

Catalyst for bioconjugate synthesis by Click Chemistry.

General properties

Appearance: dark blue powder
Molecular weight: 803.00
CAS number: 2242791-82-6 (potassium salt); 2130955-10-9 (inner salt); 2242791-81-5 (inner salt); 2301999-22-2 (sodium salt)
Molecular formula: C38H43KN4O9S2
Solubility: soluble in water, DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95+%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 271000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.28
CF260: 0.04
CF280: 0.04

Product citations

  1. Baranova, N.; Radler, P.; Hernandez-Rocamora, V.M.; Alfonso, C.; Lopez-Pelegrin, M.; Rivas, G.; Vollmer, W.; Loose, M. FtsZ assembles the bacterial cell division machinery by a diffusion-and-capture mechanism. bioRxiv, preprint. doi: 10.1101/485656
  2. Stankowska, D.L.; Nam, M.-H.; Nahomi, R.B.; Chaphalkar, R.M.; Nandi, S.K.; Fudala, R.; Krishnamoorthy, R.R.; Nagaraj, R.H. Systemically administered peptain-1 inhibits retinal ganglion cell death in animal models: implications for neuroprotection in glaucoma. Cell Death Discovery, 2019, 5(1), 112. doi: 10.1038/s41420-019-0194-2
  3. Graham, T.G.W.; Carney, S.M.; Walter, J.C.; Loparo, J.J. A single XLF dimer bridges DNA ends during nonhomologous end joining. Nature Structural & Molecular Biology, 2018, 25(9), 877–884. doi: 10.1038/s41594-018-0120-y
  4. Torres, O.B.; Duval, A.J.; Sulima, A.; Antoline, J.F.G.; Jacobson, A.E.; Rice, K.C.; Alving, C.R.; Matyas, G.R. A rapid solution-based method for determining the affinity of heroin hapten-induced antibodies to heroin, its metabolites, and other opioids. Analytical and Bioanalytical Chemistry, 2018, 410(16), 3885–3903. doi: 10.1007/s00216-018-1060-4
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