ATT 647N in Fluorophores

Absorption max 644 nm Emission max 662 nm

Fluorophore chart

ATT 647N is a rhodamine-based far-red fluorophore with strong molar absorption, high fluorescence quantum yield, and excellent thermal and photostability. ATT 647N fluorescence is independent of pH in the range of 2 to 11, which supports its application under diverse experimental conditions.

The dye is moderately hydrophilic. Upon conjugation to a substrate, the dye becomes cationic, carrying a net positive charge of +1.

Unlike cyanine dyes, ATT 647N exhibits enhanced resistance to atmospheric ozone degradation, making it highly suitable for microarray and other high-precision applications such as single-molecule detection, super-resolution microscopy techniques (e.g., SIM and STED), flow cytometry (FACS), and fluorescence in situ hybridization (FISH).

ATT 647N azide

Far-red fluorescent ATT 647N dye derivative functionalized with an azide group for copper-catalyzed or copper-free click chemistry with alkyne-bearing biomolecules.

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