dsDNA quantification calculator

Use this calculator for fast and easy quantification of dsDNA with our PreciseGreen DNA quantification kit. The linear regression tool can be used for any type of quantification. The dilution series and recommendations also fit the most popular format of dsDNA quantification reagents.

Cuvette volume: µL

1. Preparation of buffer and PreciseGreen working solutions

  • Dilute 592 µL of the 20× TE buffer stock solution (part of the kit) with 11.248 mL of deionized water to obtain of 1× ТЕ buffer.
  • After thawing, dilute of the PreciseGreen dye stock solution (part of the kit) 200-fold with of 1× ТЕ buffer. (PreciseGreen is also available separately as quantification reagent.)
  • Mix and use up within the next 3 hours.

2. dsDNA standard serial dilution

  • Mix of a ng/µL dsDNA standard solution (a 100 ng/µL solution is part of the kit) with of 1× TE buffer to obtain a dsDNA stock solution of 2 ng/µL.
  • Further dilute this dsDNA stock solution with 1× TE buffer and add the PreciseGreen working solution according to the following table.
    #1× ТЕ buffer, µLdsDNA stock solutionPreciseGreen working solution, µLFinal dsDNA concentration, pg/µL

3. dsDNA sample dilution and fluorescence measurements

  • Add µL of your DNA sample to of 1× TE buffer, then add of the PreciseGreen working solution. Mix and incubate for 5 min.
  • Measure the fluorescence intensity of standard and sample DNA solutions in a fluorometer. Excitation maximum is at 503 nm, emission maximum at 525 nm.
    #Concentration, pg/µLFluorescence intensity, a.u.Calibration chart
    Calibration data
    #1  
     DNA SampleDNA sample dilution
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