Pico488 dsDNA quantification reagent, 200x solution in DMSO

PCR and qPCR Nucleic acid quantification
Cat. # Quantity Price Lead time
12010 100 uL $39.00 in stock
42010 1 mL $290.00 in stock
62010 25 mL please inquire in stock
92010 50 mL please inquire in stock
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Pico488 is a DNA binding benzothiazole dye whose structure is identical to PicoGreen®. This reagent is a 200× solution in DMSO. The dye is a fluorescent sensor that is specific for double stranded DNA (dsDNA). Its absorption and emission in the fluorescein channel can be easily detected using cuvette fluorometers, well plate readers, and by dedicated DNA measurement fluorometers like Qubit®.

The dye exhibits a linear response of fluorescence to dsDNA concentrations in the range of low pg/mL to high ng/mL. The ready kit including quantification dye is also available.

Pico488 dsDNA quantification reagent, 200x solution in DMSO, 42010
Pico488 dsDNA quantification reagent, 200x solution in DMSO, 42010
Pico488 dsDNA quantification reagent, 200x solution in DMSO, 42010
Pico488 fluorescence emission vs DNA concentration
Fluorescence of the Pico488 Quantitation Reagent upon binding with dsDNA, ssDNA and RNA
Structure of Pico488 (PicoGreen®)
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Pico488 (Pico Green) excitation and emission spectra

Pico488 (Pico Green) excitation and emission spectra

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General properties

Appearance: orange solution
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 503
Emission maximum, nm: 526

Product citations

  1. Norred, S.E.; Dabbs, R.M.; Chauhan, G.; Caveney, P.M.; Collier, C.P.; Abel, S.M.; Simpson, M.L. Synergistic interactions between confinement and macromolecular crowding spatially order transcription and translation in cell-free expression. bioRxiv, preprint. doi: 10.1101/445544
  2. Morozov, V.N.; Klimovich, M.A.; Kolyvanova, M.A.; Dement'eva, O.V.; Rudoy, V.M.; Kuzmin, V.A. Interaction of Gold Nanoparticles with Cyanine Dyes in Cholesteric DNA Submicroparticles. High Energy Chemistry, 2021, 55(5), 341–348. doi: 10.1134/S0018143921050088
  3. Wiktor, J.; Gynnå, A.H.; Leroy, P.; Larsson, J.; Coceano, G.; Testa, I.; Elf, J. RecA finds homologous DNA by reduced dimensionality search. Nature, 2021, 597(7876), 426–429. doi: 10.1038/s41586-021-03877-6
  4. Morozov, V.N.; Kolyvanova, M.A.; Dement'eva, O.V.; Rudoy, V.M.; Kuzmin, V.A. Comparison of quenching efficacy of SYBR green I and picoGreen fluorescence by ultrasmall gold nanoparticles in isotropic and liquid-crystalline DNA systems. Journal of Molecular Liquids, 2021, 321, 114751. doi: 10.1016/j.molliq.2020.114751
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PicoGreen® and Qubit® are trademarks of Molecular Probes, Inc.
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