Pico488 dsDNA quantification reagent, 200x solution in DMSO
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 12010 | 100 uL | $39.00 | in stock | |
| 42010 | 1 mL | $290.00 | in stock | |
| 62010 | 25 mL |
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|
in stock | |
| 92010 | 50 mL |
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|
5 days |
Pico488 is a DNA binding benzothiazole dye whose structure is identical to PicoGreen®. This reagent is a 200× solution in DMSO. The dye is a fluorescent sensor that is specific for double stranded DNA (dsDNA). Its absorption and emission in the fluorescein channel can be easily detected using cuvette fluorometers, well plate readers, and by dedicated DNA measurement fluorometers like Qubit®.
The dye exhibits a linear response of fluorescence to dsDNA concentrations in the range of low pg/mL to high ng/mL. The ready kit including quantification dye is also available.
Pico488 (Pico Green) excitation and emission spectra
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General properties
| Appearance: | orange solution |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 503 |
| Emission maximum, nm: | 526 |
Product citations
- Norred, S.E.; Dabbs, R.M.; Chauhan, G.; Caveney, P.M.; Collier, C.P.; Abel, S.M.; Simpson, M.L. Synergistic interactions between confinement and macromolecular crowding spatially order transcription and translation in cell-free expression. bioRxiv, preprint. doi: 10.1101/445544
- Wiktor, J.; Gynnå, A.H.; Leroy, P.; Larsson, J.; Coceano, G.; Testa, I.; Elf, J. RecA finds homologous DNA by reduced dimensionality search. Nature, 2021, 597(7876), 426–429. doi: 10.1038/s41586-021-03877-6
- Morozov, V.N.; Kolyvanova, M.A.; Dement'eva, O.V.; Rudoy, V.M.; Kuzmin, V.A. Comparison of quenching efficacy of SYBR green I and picoGreen fluorescence by ultrasmall gold nanoparticles in isotropic and liquid-crystalline DNA systems. Journal of Molecular Liquids, 2021, 321, 114751. doi: 10.1016/j.molliq.2020.114751
- Cuccaro, A.; De Marchi, L.; Oliva, M.; Sanches, M.V.; Freitas, R.; Casu, V.; Monni, G.; Miragliotta, V.; Pretti, C. Sperm quality assessment in Ficopomatus enigmaticus (Fauvel, 1923): Effects of selected organic and inorganic chemicals across salinity levels. Ecotoxicology and Environmental Safety, 2021, 207, 111219. doi: 10.1016/j.ecoenv.2020.111219
PicoGreen® and Qubit® are trademarks of Molecular Probes, Inc.
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