Pico488 dsDNA quantification reagent, 200x solution in DMSO

Cat. # Quantity Price Lead time
12010 100 uL $39.00 in stock
42010 1 mL $290.00 in stock

Pico488 is a DNA binding benzothiazole dye whose structure is identical to PicoGreen®. This reagent is a 200× solution in DMSO. The dye is a fluorescent sensor that is specific for double stranded DNA (dsDNA). Its absorption and emission in the fluorescein channel can be easily detected using cuvette fluorometers, well plate readers, and by dedicated DNA measurement fluorometers like Qubit®.

The dye exhibits a linear response of fluorescence to dsDNA concentrations in the range of low pg/mL to high ng/mL. The ready kit including quantification dye is also available.

Pico488 (Pico Green) excitation and emission spectra

Pico488 (Pico Green) excitation and emission spectra

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General properties

Appearance: orange solution
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 503
Emission maximum, nm: 526

Product citations

  1. Norred, S.E.; Dabbs, R.M.; Chauhan, G.; Caveney, P.M.; Collier, C.P.; Abel, S.M.; Simpson, M.L. Synergistic interactions between confinement and macromolecular crowding spatially order transcription and translation in cell-free expression. bioRxiv, preprint. doi: 10.1101/445544
  2. Kosmider, B.; Lin, C.-R.; Karim, L.; Tomar, D.; Vlasenko, L.; Marchetti, N.; Bolla, S.; Madesh, M.; Criner, G.J.; Bahmed, K. Mitochondrial dysfunction in human primary alveolar type II cells in emphysema. EBioMedicine, 2019, 46, 305–316. doi: 10.1016/j.ebiom.2019.07.063
  3. Pantelidou, C.; Sonzogni, O.; De Oliveria Taveira, M.; Mehta, A.K.; Kothari, A.; Wang, D.; Visal, T.; Li, M.K.; Pinto, J.; Castrillon, J.A.; Cheney, E.M.; Bouwman, P.; Jonkers, J.; Rottenberg, S.; Guerriero, J.L.; Wulf, G.M.; Shapiro, G.I. PARP Inhibitor Efficacy Depends on CD8+ T-cell Recruitment via Intratumoral STING Pathway Activation in BRCA-Deficient Models of Triple-Negative Breast Cancer. Cancer Discovery, 2019, 9(6), 722–737. doi: 10.1158/2159-8290.CD-18-1218
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