Pyrene phosphoramidite dU

Cat. # Quantity Price Lead time
11460 100 mg $150.00 in stock
21460 250 mg $320.00 in stock
41460 1 g $880.00 in stock
61460 5 g please inquire in stock
81460 10 g please inquire 10 days

Pyrene is polycyclic aromatic hydrocarbon which is well-known for its ability to intercalate into DNA. Pyrene features intense blue fluorescence. Its parameters depend strongly on fluorophore microenvironment. Therefore, fluorescence spectra of pyrene are used for the extraction of structural information about site surrounding pyrene. Two pyrenes in close proximity usually form excimers easily detectable by excimer fluorescence. Pyrene can also be a FRET donor to other fluorophores such as perylene.

With this phosphoramidite, pyrene can be introduced into DNA by means of automated synthesis. This pyrene phosphoramidite contains hydrocarbon moiety rigidly attached to deoxyuridine. This reagent allows to attach pyrene fragment to 5', internal, or 3' position (using universal support).

This amidite requires no special handling, coupling, or deprotection conditions. Recommended diluent for it is acetonitrile.

Absorption and emission spectra of pyrene fluorophore

Absorption and emission spectra of pyrene fluorophore

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General properties

Appearance: yellowish foam
Molecular weight: 955.04
CAS number: 199920-17-7
Molecular formula: C57H55N4O8P
Solubility: good in dichloromethane and acetonitrile
Quality control: NMR 1H (95%), 31P, HPLC-MS
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 260; 282; 365; 392
ε, L⋅mol−1⋅cm−1: 12600; 21900; 16000; 14200
Emission maximum, nm: 460
Fluorescence quantum yield: ~0.1

Oligo synthesis details

Diluent: acetonitrile
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