Pyrenebutyric acid NHS ester

Cat. # Quantity Price Lead time
63520 100 mg $110.00 in stock

Pyrene is a polyaromatic hydrocarbon which exhibits fluorescence in blue range of the spectrum. When two pyrene residues are closely spaced, excimer fluorescence is observed at longer wavelength. Therefore, pyrene is useful as a proximity label.

Pyrene exhibits affinity to graphite, and can be used as an anchor group for graphite. This reagent can be conjugated with biomolecules for their subsequent immobilization on graphite surfaces.

This NHS ester allows to label biomolecules with amino groups, such as proteins, peptides, amino-DNA and other molecules.

Pyrene is also a FRET donor for perylene.

Absorption and emission spectra of pyrene fluorophore

Absorption and emission spectra of pyrene fluorophore

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Coumarin 343 X NHS ester

Coumarin 343 is a blue emitting dye that can serve as a FRET donor for fluorescein (FAM). This structure features a linker between Coumarin 343 fluorophore, and the functional group. It provides better solubility, and spatial separation between the dye, and the functional group.

General properties

Appearance: off-white solid
Mass spec M+ increment: 270.1
Molecular weight: 385.41
CAS number: 114932-60-4
Molecular formula: C24H19NO4
IUPAC name: 1-​Pyrenebutanoic acid, 2,​5-​dioxo-​1-​pyrrolidinyl ester
Solubility: good in DCM, chloroform, moderate in DMF, DMSO, low in water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 343; 326; 313; 276; 265; 242; 234
Emission maximum, nm: 377; 397
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