FAM azide, 6-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 15130 | 100 uL, 10 mM/DMSO | $80.00 | in stock | |
| 35130 | 500 uL, 10 mM/DMSO | $100.00 | in stock | |
| 45130 | 1 mL, 10 mM/DMSO | $150.00 | in stock | |
| A5130 | 1 mg | $80.00 | in stock | |
| B5130 | 5 mg | $100.00 | in stock | |
| C5130 | 10 mg | $150.00 | 5 days | |
| D5130 | 25 mg | $295.00 | in stock | |
| E5130 | 50 mg |
$450.00
|
in stock | |
| F5130 | 100 mg |
$780.00
|
in stock |
FAM (fluorescein) azide. solid and 10 mM solution in DMSO, labeling reagent for Click chemistry.
Pure 6-isomer.
Can replace Alexa Fluor 488, DyLight 488.
Recommended protocol
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General properties
| Appearance: | yellowish crystals |
| Molecular weight: | 458.42 |
| CAS number: | 1386385-76-7 |
| Molecular formula: | C24H18N4O6 |
| Solubility: | soluble in polar organic solvents (DMF, DMSO, alcohols) |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 494 |
| ε, L⋅mol−1⋅cm−1: | 75000 |
| Emission maximum, nm: | 520 |
| Fluorescence quantum yield: | 0.9 |
| CF260: | 0.20 |
| CF280: | 0.17 |
Product citations
- Haider, N.; Dutt, P.; van de Kooij, B.; Yaffe, M.B.; Stambolic, V. NEK10 tyrosine phosphorylates p53 and controls its transcriptional activity. bioRxiv. doi: 10.1101/516971
- Feltes, M.; Moores, S.; Gale, S.E.; Krishnan, K.; Mydock-McGrane, L.; Covey, D.F.; Ory, D.S.; Schaffer, J.E. Synthesis and characterization of diazirine alkyne probes for the study of intracellular cholesterol trafficking. Journal of Lipid Research, in press. doi: 10.1194/jlr.D091470
- Ruhl, K.E; Rovis, T. Visible Light-Gated Cobalt Catalysis for a Spatially and Temporally Resolved [2+2+2] Cycloaddition. Journal of the American Chemical Society, 2016, 138(48), 15527–15530. doi: 10.1021/jacs.6b08792
- Berte, N.; Piee-Staffa, A.; Piecha, N.; Wang, M.; Borgmann, K.; Kaina, B.; Nikolova, T. Targeting homologous recombination by pharmacological inhibitors enhances the killing response of glioblastoma cells treated with alkylating drugs. Molecular Cancer Therapeutics, 2016, 15(11), 2665–2678. doi: 10.1158/1535-7163.mct-16-0176
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