FAM azide, 6-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 15130 | 100 uL, 10 mM/DMSO | $80.00 | in stock | |
| 35130 | 500 uL, 10 mM/DMSO | $100.00 | in stock | |
| 45130 | 1 mL, 10 mM/DMSO | $150.00 | in stock | |
| A5130 | 1 mg | $80.00 | in stock | |
| B5130 | 5 mg | $100.00 | in stock | |
| C5130 | 10 mg | $150.00 | in stock | |
| D5130 | 25 mg | $295.00 | in stock | |
| E5130 | 50 mg |
$450.00
|
in stock | |
| F5130 | 100 mg |
$780.00
|
in stock |
FAM (fluorescein) azide. solid and 10 mM solution in DMSO, labeling reagent for Click chemistry.
Pure 6-isomer.
Can replace Alexa Fluor 488, DyLight 488.
Recommended protocol
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Reagent for fluorescent labeling with pyrene polycyclic aromatic hydrocarbon via Click Chemistry.General properties
| Appearance: | yellowish crystals |
| Molecular weight: | 458.42 |
| CAS number: | 1386385-76-7 |
| Molecular formula: | C24H18N4O6 |
| Solubility: | soluble in polar organic solvents (DMF, DMSO, alcohols) |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 494 |
| ε, L⋅mol−1⋅cm−1: | 75000 |
| Emission maximum, nm: | 520 |
| Fluorescence quantum yield: | 0.9 |
| CF260: | 0.20 |
| CF280: | 0.17 |
Product citations
- Lee, R.; Erstling, J.A.; Hinckley, J.A.; Chapman, D.V.; Wiesner, U.B. Addressing Particle Compositional Heterogeneities in Super-Resolution-Enhanced Live-Cell Ratiometric pH Sensing with Ultrasmall Fluorescent Core–Shell Aluminosilicate Nanoparticles. Advanced Functional Materials, in press. doi: 10.1002/adfm.202106144
- Haider, N.; Dutt, P.; van de Kooij, B.; Yaffe, M.B.; Stambolic, V. NEK10 tyrosine phosphorylates p53 and controls its transcriptional activity. bioRxiv, preprint. doi: 10.1101/516971
- Ramey-Ward, A.N.; Su, H.; Salaita, K. Mechanical stimulation of adhesion receptors using light-responsive nanoparticle actuators enhances myogenesis. ACS Applied Materials & Interfaces, 2020, 12(32), 35903–35917. doi: 10.1021/acsami.0c08871
- Reed, S.A.; Brzovic, D.A.; Takasaki, S.S.; Boyko, K.V.; Antos, J.M. Efficient Sortase-Mediated Ligation Using a Common C-Terminal Fusion Tag. Bioconjugate Chemistry, 2020, 31(5), 1463–1473. doi: 10.1021/acs.bioconjchem.0c00156
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