FAM azide, 6-isomer

Fluorescein (FAM) Azides Fluorescence polarization Microscopy
Cat. # Quantity Price Lead time
15130 100 uL, 10 mM/DMSO $80.00 in stock
35130 500 uL, 10 mM/DMSO $100.00 in stock
45130 1 mL, 10 mM/DMSO $150.00 in stock
A5130 1 mg $80.00 5 days
B5130 5 mg $100.00 in stock
C5130 10 mg $150.00 in stock
D5130 25 mg $295.00 in stock
E5130 50 mg $450.00 in stock
F5130 100 mg $780.00 in stock
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FAM (fluorescein) azide. solid and 10 mM solution in DMSO, labeling reagent for Click chemistry.

Pure 6-isomer.

Can replace Alexa Fluor 488, DyLight 488.

FAM azide, 6-isomer, F5130
FAM azide, 6-isomer, F5130
FAM azide, 6-isomer, F5130
FAM azide, 6-isomer, azide stock solution for click chemistry (10 mM in DMSO), 35130
FAM azide, 6-isomer, azide stock solution for click chemistry (10 mM in DMSO), 35130
FAM azide, 6-isomer, azide stock solution for click chemistry (10 mM in DMSO), 35130
6-FAM azide structure
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General properties

Appearance: yellowish crystals
Molecular weight: 458.42
CAS number: 1386385-76-7
Molecular formula: C24H18N4O6
Solubility: soluble in polar organic solvents (DMF, DMSO, alcohols)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 494
ε, L⋅mol−1⋅cm−1: 75000
Emission maximum, nm: 520
Fluorescence quantum yield: 0.9
CF260: 0.20
CF280: 0.17

Product citations

  1. Haider, N.; Dutt, P.; van de Kooij, B.; Yaffe, M.B.; Stambolic, V. NEK10 tyrosine phosphorylates p53 and controls its transcriptional activity. bioRxiv, preprint. doi: 10.1101/516971
  2. Feltes, M.; Moores, S.; Gale, S.E.; Krishnan, K.; Mydock-McGrane, L.; Covey, D.F.; Ory, D.S.; Schaffer, J.E. Synthesis and characterization of diazirine alkyne probes for the study of intracellular cholesterol trafficking. Journal of Lipid Research, 2019, 60(3), 707–716. doi: 10.1194/jlr.D091470
  3. Ruhl, K.E; Rovis, T. Visible Light-Gated Cobalt Catalysis for a Spatially and Temporally Resolved [2+2+2] Cycloaddition. Journal of the American Chemical Society, 2016, 138(48), 15527–15530. doi: 10.1021/jacs.6b08792
  4. Berte, N.; Piee-Staffa, A.; Piecha, N.; Wang, M.; Borgmann, K.; Kaina, B.; Nikolova, T. Targeting homologous recombination by pharmacological inhibitors enhances the killing response of glioblastoma cells treated with alkylating drugs. Molecular Cancer Therapeutics, 2016, 15(11), 2665–2678. doi: 10.1158/1535-7163.mct-16-0176
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