FAM azide, 6-isomer
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
15130 | 100 uL, 10 mM/DMSO | $80.00 | in stock | |
35130 | 500 uL, 10 mM/DMSO | $100.00 | in stock | |
45130 | 1 mL, 10 mM/DMSO | $150.00 | in stock | |
A5130 | 1 mg | $80.00 | in stock | |
B5130 | 5 mg | $100.00 | in stock | |
C5130 | 10 mg | $150.00 | in stock | |
D5130 | 25 mg | $295.00 | in stock | |
E5130 | 50 mg |
$450.00
|
in stock | |
F5130 | 100 mg |
$780.00
|
in stock |
FAM (fluorescein) azide. solid and 10 mM solution in DMSO, labeling reagent for Click chemistry.
Pure 6-isomer.
Can replace Alexa Fluor 488, DyLight 488.
Recommended protocol
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EdU is a nucleoside which is incorporated into replicated DNA by cellular enzymes. After it, DNA contained in the cells can be developed by Click chemistry reaction with fluorescent dye azides to reveal cell proliferation.Sulfo-Cyanine3 azide
Water-soluble sulfo-Cyanine3 dye azide for Click Chemistry.BDP TMR azide
Borondipyrromethene dye with azido group. Spectral properties are similar to TAMRA dye. Due to long fluorescence lifetime, BDP TMR is especially useful for fluorescence polarization measurements.General properties
Appearance: | yellowish crystals |
Molecular weight: | 458.42 |
CAS number: | 1386385-76-7 |
Molecular formula: | C24H18N4O6 |
Solubility: | soluble in polar organic solvents (DMF, DMSO, alcohols) |
Quality control: | NMR 1H, HPLC-MS (95%) |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation maximum, nm: | 494 |
ε, L⋅mol−1⋅cm−1: | 75000 |
Emission maximum, nm: | 520 |
Fluorescence quantum yield: | 0.9 |
CF260: | 0.20 |
CF280: | 0.17 |
Product citations
- Haider, N.; Dutt, P.; van de Kooij, B.; Yaffe, M.B.; Stambolic, V. NEK10 tyrosine phosphorylates p53 and controls its transcriptional activity. bioRxiv, preprint. doi: 10.1101/516971
- Ramey-Ward, A.N.; Su, H.; Salaita, K. Mechanical stimulation of adhesion receptors using light-responsive nanoparticle actuators enhances myogenesis. ACS Applied Materials & Interfaces, 2020, 12(32), 35903–35917. doi: 10.1021/acsami.0c08871
- Reed, S.A.; Brzovic, D.A.; Takasaki, S.S.; Boyko, K.V.; Antos, J.M. Efficient Sortase-Mediated Ligation Using a Common C-Terminal Fusion Tag. Bioconjugate Chemistry, 2020, 31(5), 1463–1473. doi: 10.1021/acs.bioconjchem.0c00156
- Morais, C.M.; Cunha, P.P.; Melo, T.; Cardoso, A.M.; Domingues, P.; Domingues, M.R.; Pedroso de Lima, M.C.; Jurado, A.S. Glucosylceramide synthase silencing combined with the receptor tyrosine kinase inhibitor axitinib as a new multimodal strategy for glioblastoma. Human Molecular Genetics, 2019, 28(21), 3664–3679. doi: 10.1093/hmg/ddz152
