Thiazole Orange NHS ester

Cat. # Quantity Price Lead time
2183-1mg 1 mg –   in stock
2183-5mg 5 mg $60 in stock
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Thiazol Orange (TO) is an asymmetric cyanine dye whose fluorescence highly depends on the local environment. TO is essentially dark in solution; however, its fluorescence increases a thousandfold when ТО is introduced into double-stranded DNA and RNA (dsDNA or dsRNA). When TO-labeled oligonucleotide is hybridized to its complementary sequence, TO acts as an intercalator.

TO fluorescence is also induced by interaction with supramolecular hosts and amino acid side chains, which allows it to be used for detecting not only nucleic acids and proteins but also other analytes such as ions and small molecules.

The maximum absorption of TO with DNA is 509 nm, and the maximum emission is 532 nm.

Thiazole Orange NHS ester effectively reacts with amines and is well suited for labeling biomolecules (peptides, proteins, amino-DNA, etc.) in an aqueous medium. Thiazole Orange NHS ester can be useful for studying the binding of nucleic acids to other biomolecules, such as DNA-binding proteins.

Absorption and emission spectra of Thiazole Orange

Absorption and emission spectra of Thiazole Orange

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General properties

Appearance: orange powder
Molecular weight: 538.07
Molecular formula: C28H28ClN3O4S
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 509
Emission maximum, nm: 532
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