Cyanine5 NHS ester
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 13020 | 1 mg | $110.00 | in stock | |
| 23020 | 5 mg | $210.00 | in stock | |
| 43020 | 25 mg |
$410.00
|
in stock | |
| 53020 | 50 mg |
$695.00
|
in stock | |
| 63020 | 100 mg |
$1190.00
|
in stock |
During the last years, Cyanine5 (an analog of Cy5®) has become an incredibly popular label in life science research and diagnostics. The fluorophore has its emission maximum in the red region, where many CCD detectors exhibit maximum sensitivity, and biological objects show low background. The dye color is very intense, therefore quantities as small as 1 nmol can be detected in gel electrophoresis by naked eye.
This Cyanine5 NHS ester (analog to Cy5® NHS ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires a small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reactions (please see our recommended protocol for more details). This reagent is ideal for very cost-efficient labeling of soluble proteins as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling. For more sophisticated targets such as easily degradable proteins, when the use of DMF or DMSO is undesirable, consider using water-soluble sulfo-Cyanine 5 NHS ester which does not require any co-solvent, and features very similar fluorescent properties.
Cyanine5 fluorophore is compatible with various instrumentation including many fluorescent microscopes, imagers, scanners, and fluorescence readers. A number of various Cyanine5 analogs exist - Cyanine5 NHS ester can replace activated esters of Cy5®, Alexa Fluor 647, and DyLight 649.
Cy5 excitation and emission spectra
Recommended protocol
Calculator
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Fluorescein dye alkyne for Click Chemistry, pure 6-isomer.Alkyne hydrazide
Bifunctional reagent containing alkyne group and hydrazide function. It reacts with aldehydes and ketones. Alkyne group can then be coupled with various azides via CuAAC reaction.Sulfo-Cyanine5.5 carboxylic acid
Water soluble far red fluorescent dye sulfo-Cyanine5.5, free non-activated carboxylic acid. Useful for the calibration, and as a non-reactive control.General properties
| Appearance: | dark blue solid |
| Molecular weight: | 667.54 |
| CAS number: | 1263093-76-0 (tetrafluoroborate), 1032678-42-4 (chloride), 350686-88-3 (without anion) |
| Molecular formula: | C36H42N3BF4O4 |
| IUPAC name: | 3H-Indolium, 2-[5-(1,3-dihydro-1,3,3-trimethyl-2H-indol-2-ylidene)-1,3-pentadien-1-yl]-1-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-3,3-dimethyl-, tetrafluoroborate |
| Solubility: | very poorly soluble in water (0.19 mM = 127 mg/L), good in polar (DMSO, DMF) and chlorinated (DCM, chloroform) organic solvents |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 250000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.2 |
| CF260: | 0.03 |
| CF280: | 0.04 |
Product citations
- Jiang, S.; Mottola, M.; Han, S.; Thiramanas, R.; Graf, R.; Lieberwirth, I.; Mailänder, V.; Crespy, D.; Landfester, K. Versatile Preparation of Silica Nanocapsules for Biomedical Applications. Particle & Particle Systems Characterization, in press. doi: 10.1002/ppsc.201900484
- Palumbo, C.M.; Gutierrez-Bujari, J.M.; O'Geen, H.; Segal, D.J.; Beal, P. Versatile 3' Functionalization of CRISPR Single Guide RNA. ChemBioChem, in press. doi: 10.1002/cbic.201900736
- Janiszewski, T.; Kołt, S.; Kaiserman, D.; Snipas, S.; Li, S.; Kulbacka, J.; Saczko, J.; Bovenschen, N.; Salvesen, G.; Drąg, M.; Bird, P.I.; Kasperkiewicz, P. Noninvasive optical detection of Granzyme B from natural killer cells using enzyme-activated fluorogenic probes. bioRxiv, preprint. doi: 10.1101/2019.12.16.875070
- Poreba, M.; Groborz, K.; Rut, W.; Pore, M.; Snipas, S.J.; Vizovisek, M.; Turk, B.; Kuhn, P.; Drag, M.; Salvesen, G.S. The Activome: multiplexed probing of activity of proteolytic enzymes using mass cytometry-compatible activity-based probes (TOF-probes). bioRxiv, preprint. doi: 10.1101/775627
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