Cyanine5 NHS ester
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 13020 | 1 mg | $110.00 | in stock | |
| 23020 | 5 mg | $210.00 | in stock | |
| 43020 | 25 mg |
$410.00
|
in stock | |
| 53020 | 50 mg |
$695.00
|
in stock | |
| 63020 | 100 mg |
$1190.00
|
in stock |
During the last years, Cyanine5 (an analog of Cy5®) has become an incredibly popular label in life science research and diagnostics. The fluorophore has its emission maximum in the red region, where many CCD detectors exhibit maximum sensitivity, and biological objects show low background. The dye color is very intense, therefore quantities as small as 1 nmol can be detected in gel electrophoresis by naked eye.
This Cyanine5 NHS ester (analog to Cy5® NHS ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires a small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reactions (please see our recommended protocol for more details). This reagent is ideal for very cost-efficient labeling of soluble proteins as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling. For more sophisticated targets such as easily degradable proteins, when the use of DMF or DMSO is undesirable, consider using water-soluble sulfo-Cyanine 5 NHS ester which does not require any co-solvent, and features very similar fluorescent properties.
Cyanine5 fluorophore is compatible with various instrumentation including many fluorescent microscopes, imagers, scanners, and fluorescence readers. A number of various Cyanine5 analogs exist - Cyanine5 NHS ester can replace activated esters of Cy5®, Alexa Fluor 647, and DyLight 649.
Cy5 excitation and emission spectra
Recommended protocol
Calculator
Customers also purchased with this product
Sulfo-Cyanine3 alkyne
A water soluble dye alkyne for copper-catalyzed Click Chemistry. The fluorophore Sulfo-Cyanine3 is a bright and photostable dye for the Cy3® channel.Cyanine5.5 alkyne
Cyanine5.5 alkyne is a NIR (near infrared) dye azide for both copper-catalyzed, and copper-free Click Chemistry.PEP azide
PEP (phenylethynylpyrene), polyaromatic hydrocarbon fluorescent dye for Click-Chemistry labeling.General properties
| Appearance: | dark blue solid |
| Molecular weight: | 667.54 |
| CAS number: | 1263093-76-0 (tetrafluoroborate), 1032678-42-4 (chloride), 350686-88-3 (without anion) |
| Molecular formula: | C36H42N3BF4O4 |
| IUPAC name: | 3H-Indolium, 2-[5-(1,3-dihydro-1,3,3-trimethyl-2H-indol-2-ylidene)-1,3-pentadien-1-yl]-1-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-3,3-dimethyl-, tetrafluoroborate |
| Solubility: | good in polar (DMSO, DMF) and chlorinated (DCM, chloroform) organic solvents, low solubility in water |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 250000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.2 |
| CF260: | 0.03 |
| CF280: | 0.04 |
Product citations
- Lin, P.-Y.; Chen, K.-H.; Miao, Y.-B.; Chen, H.-L.; Lin, K.-J.; Chen, C.-T.; Yeh, C.-N.; Chang, Y.; Sung, H.-W. Phase-Changeable Nanoemulsions for Oral Delivery of a Therapeutic Peptide: Toward Targeting the Pancreas for Antidiabetic Treatments Using Lymphatic Transport. Advanced Functional Materials, in press. doi: 10.1002/adfm.201809015
- Madl, C.M.; LeSavage, B.L.; Dewi, R.E.; Lampe, K.J.; Heilshorn, S.C. Matrix Remodeling Enhances the Differentiation Capacity of Neural Progenitor Cells in 3D Hydrogels. Advanced Science, in press. doi: 10.1002/advs.201801716
- Xiao, C.; Hu, H.; Yang, H.; Li, S.; Zhou, H.; Ruan, J.; Zhu, Y.; Yang, X.; Li, Z. Colloidal hydroxyethyl starch for tumor-targeted platinum delivery. Nanoscale Advances, in press. doi: 10.1039/c8na00271a
- Liu, Y.; Li, Q.; Xiong, X.; Zhou, Z. Improved mitochondrial targeting effect of HPMA copolymer by SS20 peptide mediation and nonendocytosis pathway. Journal of Peptide Science, 2019, 25(2), e4133. doi: 10.1002/psc.3144
Your cart is empty
$ 
