FAM tetrazine, 6-isomer

Cat. # Quantity Price Lead time
151E0 1 mg
$110
in stock
251E0 5 mg
$210
in stock
451E0 25 mg
$410
in stock
551E0 50 mg
$695
in stock
651E0 100 mg
$1190
in stock
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Fluorescein (FAM) is a popular fluorophore that has bright emission in the green area of the visible spectrum. This fluorescein derivative contains tetrazine moiety that reacts with trans-cycloalkenes and other strained olefins in inverse electron demand Diels-Alder reaction (IEDDA). The reaction is very quick and specific. This reagent is a pure 6-isomer of FAM.

Absorption and emission spectra of FAM

Absorption and emission spectra of FAM

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FAM tetrazine, 5-isomer

FAM tetrazine (methyltetrazine) for the conjugation with trans-cyclooctenes and cyclopropenes. Pure 5-isomer of fluorescein.

General properties

Appearance: orange solid
Mass spec M+ increment: 531.1
Molecular weight: 559.53
Molecular formula: C31H21N5O6
Solubility: good in DMF, DMSO, poor in water
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 492
ε, L⋅mol−1⋅cm−1: 74000
Emission maximum, nm: 517
Fluorescence quantum yield: 0.93
CF260: 0.22
CF280: 0.17
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