FAM tetrazine, 6-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 151E0 | 1 mg | $110.00 | in stock | |
| 251E0 | 5 mg | $210.00 | in stock | |
| 451E0 | 25 mg |
$410.00
|
in stock | |
| 551E0 | 50 mg |
$695.00
|
in stock | |
| 651E0 | 100 mg |
$1190.00
|
in stock |
Fluorescein (FAM) is a popular fluorophore that has bright emission in the green area of visible spectrum. This derivative of fluorescein contains tetrazine moiety. Tetrazines react with trans-cycloalkenes and other strained olefins in a reaction called inverse electron demand Diels-Alder reaction (ied-DA). The reaction is very quick and specific. The reagent contains pure 6-isomer of FAM.
Absorption and emission spectra of FAM
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Controlled pore glass for the synthesis of modified oligonucleotides containing 3'-fluorescein (FAM) modification.General properties
| Appearance: | orange solid |
| Mass spec M+ increment: | 531.1 |
| Molecular weight: | 559.53 |
| Molecular formula: | C31H21N5O6 |
| Solubility: | good in DMF, DMSO, poor in water |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 490 |
| ε, L⋅mol−1⋅cm−1: | 80000 |
| Emission maximum, nm: | 513 |
| Fluorescence quantum yield: | 0.93 |
| CF260: | 0.20 |
| CF280: | 0.17 |
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