TCO phosphoramidite C6

Cat. # Quantity Price Lead time
4017-250mg 250 mg $920 in stock
4017-1g 1 g please inquire 12 days
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Trans-cyclooctene (TCO) amidite is a hydroxyl reactive block used to introduce a TCO moiety into substrates containing primary or secondary hydroxyl group, and is especially useful in preparation of TCO-modified oligonucleotides.

Trans-cyclooctene readily reacts with tetrazines via inverse electron-demand Diels-Alder cycloaddition (IEDDA). TCO-Tetrazine ligation possesses ultrafast kinetics, selectivity, and long-term stability in aqueous media, which is important in low-concentration systems applications such as protein-protein conjugations, etc.

Recommendations for using the reagent:

Coupling: Standard conditions identical to normal nucleobases.

Oxidation: TCO well tolerates iodine oxidation step conditions.

Deprotection: Exclude the dimethoxytrityl (DMT) removal step and use the Dmt-ON protocol after amidite coupling and oxidation.

Cleavage: AMA mixture (concentrated aqueous ammonia/40% methylamine 1:1) for 15 min at 65°C

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General properties

Appearance: colorless to yellowish oil
Molecular weight: 469.61
Molecular formula: C24H44N3O4P
Quality control: NMR 1H, NMR 31P (95+%)
Storage conditions: 12 months after receival at -20 °C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Oligo synthesis details

Coupling conditions: Standard conditions identical to normal nucleobases
Cleavage conditions: AMA mixture (concentrated aqueous ammonia/40% methylamine 1:1) for 15 min at 65 °C
Deprotection conditions: Dmt-ON protocol
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