sulfo-Cyanine5 DBCO
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 133F0 | 1 mg | $110.00 | in stock | |
| 233F0 | 5 mg | $290.00 | in stock | |
| 433F0 | 25 mg |
$690.00
|
in stock | |
| 533F0 | 50 mg |
$1270.00
|
in stock | |
| 633F0 | 100 mg |
$1990.00
|
in stock |
Copper-free click chemistry reaction between strained cycloalkynes (cyclooctynes), and azides, is a very fast and robust reaction. It can be used for a fast labeling with fluorescent dyes. This reagent is a derivative of water soluble sulfo-Cyanine5 dye which emits in red channel. It is useful for the labeling of biomolecules in aqueous media.
Absorption and emission spectra of sulfo-Cyanine5
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General properties
| Appearance: | dark colored solid |
| Molecular weight: | 981.27 |
| Molecular formula: | C53H57N4KO8S2 |
| Solubility: | good in water, DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 271000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.28 |
| CF260: | 0.04 |
| CF280: | 0.04 |
Product citations
- Mamot, A.; Sikorski, P.J.; Siekierska, A.; de Witte, P.; Kowalska, J.; Jemielity, J. Ethylenediamine derivatives efficiently react with oxidized RNA 3′ ends providing access to mono and dually labelled RNA probes for enzymatic assays and in vivo translation. Nucleic Acids Research, 2022, 50(1), e3. doi: 10.1093/nar/gkab867
- Lim, S.; Kim, W.; Song, S.; Shim, M.K.; Yoon, H.Y.; Kim, B.-S.; Kwon, I.C.; Kim, K. Intracellular Uptake Mechanism of Bioorthogonally Conjugated Nanoparticles on Metabolically Engineered Mesenchymal Stem Cells. Bioconjugate Chemistry, 2021, 32(1), 199–214. doi: 10.1021/acs.bioconjchem.0c00640
- Desai, B.J.; Gonzalez, R.L. Multiplexed genomic encoding of non-canonical amino acids for labeling large complexes. Nature Chemical Biology, 2020, 16, 1129–1135. doi: 10.1038/s41589-020-0599-5
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