Cyanine5 alkyne

Cat. # Quantity Price Lead time
A30B0 1 mg $110.00 in stock
B30B0 5 mg $210.00 in stock
C30B0 10 mg $310.00 in stock
D30B0 25 mg $410.00 in stock
E30B0 50 mg $695.00 in stock
F30B0 100 mg $1190.00 in stock

Cyanine5 alkyne for Click Chemistry, an analog of Cy5® alkyne. With this product, deeply colored, and photostable Cyanine5 fluorophore can be attached to various molecules via Click Chemistry reaction with azides.

This alkyne is non-water soluble, but it can be dissolved in DMF or DMSO prior to reaction, and added to aqueous reaction mixture. With our labeling protocol, the labeling reaction is very efficient, and high-yielding.

Various substrates bearing azides can be used for the labeling, including azido-labeled biomolecules, polymers, and solid surfaces.

Cyanine5 absorbance and emission spectra

Cyanine5 absorbance and emission spectra

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General properties

Appearance: dark blue powder
Molecular weight: 556.18
Molecular formula: C35H42ClN3O
Solubility: good in dichloromethane, DMF, DMSO, alcohols, very poorly soluble in water (200 mg/L = 0.4 mM)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 250000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.2
CF260: 0.03
CF280: 0.04

Product citations

  1. Mietlicki-Baase, E.G.; Liberini, C.G.; Workinger, J.L.; Bonaccorso, R.L.; Borner, T.; Reiner, D.J.; Koch-Laskowski, K.; McGrath, L.E.; Lhamo, R.; Stein, L.M.; De Jonghe, B.C.; Holz, G.G.; Roth, C.L.; Doyle, R.P.; Hayes, M.R. A Vitamin B12 Conjugate of Exendin-4 Improves Glucose Tolerance Without Associated Nausea or Hypophagia in Rodents. Diabetes, Obesity & Metabolism, 2018, 20(5), 1223–1234. doi: 10.1111/dom.13222
  2. Sacoman, J.L.; Dagda, R.Y.; Burnham-Marusich, A.R.; Dagda, R.K.; Berninsone, P.M. Mitochondrial O-GlcNAc transferase (mOGT) regulates mitochondrial structure, function and survival in HeLa cells. Journal of Biological Chemistry, 2017, 292(11), 4499–4518. doi: 10.1074/jbc.M116.726752
  3. Wallat, J.D.; Czapar, A.E.; Wang, C.; Wen, A.M.; Wek, K.S.; Yu, X.; Steinmetz, N.F.; Pokorski, J.K. Optical and Magnetic Resonance Imaging Using Fluorous Colloidal Nanoparticles. Biomacromolecules, 2017, 18(1), 103–112. doi: 10.1021/acs.biomac.6b01389
  4. Zhao, T.; Li, T.; Liu, Y. Silver nanoparticle plasmonic enhanced förster resonance energy transfer (FRET) imaging of protein-specific sialylation on the cell surface. Nanoscale, 2017, 9(28), 9841–9847. doi: 10.1039/c7nr01562c
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