Cyanine5 alkyne
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A30B0 | 1 mg | $110.00 | in stock | |
| B30B0 | 5 mg | $210.00 | in stock | |
| C30B0 | 10 mg | $310.00 | in stock | |
| D30B0 | 25 mg |
$410.00
|
in stock | |
| E30B0 | 50 mg |
$695.00
|
in stock | |
| F30B0 | 100 mg |
$1190.00
|
in stock |
Cyanine5 alkyne for Click Chemistry, an analog of Cy5® alkyne. With this product, deeply colored, and photostable Cyanine5 fluorophore can be attached to various molecules via Click Chemistry reaction with azides.
This alkyne is non-water soluble, but it can be dissolved in DMF or DMSO prior to reaction, and added to aqueous reaction mixture. With our labeling protocol, the labeling reaction is very efficient, and high-yielding.
Various substrates bearing azides can be used for the labeling, including azido-labeled biomolecules, polymers, and solid surfaces.
Cyanine5 absorbance and emission spectra
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General properties
| Appearance: | dark blue powder |
| Molecular weight: | 556.18 |
| CAS number: | 1223357-57-0 |
| Molecular formula: | C35H42ClN3O |
| Solubility: | good in dichloromethane, DMF, DMSO, alcohols, very poorly soluble in water (200 mg/L = 0.4 mM) |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 250000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.2 |
| CF260: | 0.03 |
| CF280: | 0.04 |
Product citations
- Liu, M.; Apriceno, A.; Sipin, M.; Scarpa, E.; Rodriguez-Arco, L.; Poma, A.; Marchello, G.; Battaglia, G.; Angioletti-Uberti, S. Combinatorial entropy behaviour leads to range selective binding in ligand-receptor interactions. Nature Communications, 2020, 11, 4836. doi: 10.1038/s41467-020-18603-5
- Kocere, A.; Resseguier, J.; Wohlmann, J.; Skjeldal, F.M.; Khan, S.; Speth, M.; Dal, N.-J.K.; Ng, M.Y.W.; Alonso-Rodriguez, N.; Scarpa, E.; Rizzello, L.; Battaglia, G.; Griffiths, G.; Fenaroli, F. Real-time imaging of polymersome nanoparticles in zebrafish embryos engrafted with melanoma cancer cells: Localization, toxicity and treatment analysis. EBioMedicine, 2020, 58, 102902. doi: 10.1016/j.ebiom.2020.102902
- Monteiro, P.F.; Gulfam, M.; Monteiro, C.J.; Travanut, A.; Abelha, T.F.; Pearce, A.K.; Jerôme, C.; Grabowska, A.M.; Clarke, P.A.; Collins, H.M.; Heery, D.M.; Gershkovich, P.; Alexander, C. Synthesis of micellar-like terpolymer nanoparticles with reductively-cleavable cross-links and evaluation of efficacy in 2D and 3D models of triple negative breast cancer. Journal of Controlled Release, 2020, 323, 549–564. doi: 10.1016/j.jconrel.2020.04.049
- Zivanovic, J.; Kouroussis, E.; Kohl, J.B.; Adhikari, B.; Bursac, B.; Schott-Roux, S.; Petrovic, D.; Miljkovic, J.L.; Thomas-Lopez, D.; Jung, Y.; Miler, M.; Mitchell, S.; Milosevic, V.; Gomes, J.E.; Benhar, M.; Gonzales-Zorn, B.; Ivanovic-Burmazovic, I.; Torregrossa, R:; Mitchell, J.R.; Whiteman, M.; Schwarz, G.; Snyder, S.H.; Paul, B.D.; Carroll, K.S.; Filipovic, M.R. Selective Persulfide Detection Reveals Evolutionarily Conserved Antiaging Effects of S-Sulfhydration. Cell Metabolism, 2019, 30(6), 1152–1170.e13. doi: 10.1016/j.cmet.2019.10.007
Cy® is a trademark of GE Healthcare.
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