Cyanine5 alkyne
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A30B0 | 1 mg | $110.00 | in stock | |
| B30B0 | 5 mg | $210.00 | in stock | |
| C30B0 | 10 mg | $310.00 | in stock | |
| D30B0 | 25 mg |
$410.00
|
in stock | |
| E30B0 | 50 mg |
$695.00
|
in stock | |
| F30B0 | 100 mg |
$1190.00
|
in stock |
Cyanine5 alkyne for Click Chemistry, an analog of Cy5® alkyne. With this product, deeply colored, and photostable Cyanine5 fluorophore can be attached to various molecules via Click Chemistry reaction with azides.
This alkyne is non-water soluble, but it can be dissolved in DMF or DMSO prior to reaction, and added to aqueous reaction mixture. With our labeling protocol, the labeling reaction is very efficient, and high-yielding.
Various substrates bearing azides can be used for the labeling, including azido-labeled biomolecules, polymers, and solid surfaces.
Cyanine5 absorbance and emission spectra
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TAMRA azide, 5-isomer
TAMRA fluorescent dye azide for Click chemistry.TAMRA alkyne, 5-isomer
Alkyne derivative of TAMRA dye, pure 5-isomer for CuAAC.General properties
| Appearance: | dark blue powder |
| Molecular weight: | 556.18 |
| Molecular formula: | C35H42ClN3O |
| Solubility: | good in dichloromethane, DMF, DMSO, alcohols, very poorly soluble in water (200 mg/L = 0.4 mM) |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 250000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.2 |
| CF260: | 0.03 |
| CF280: | 0.04 |
Product citations
- Mietlicki-Baase, E.G.; Liberini, C.G.; Workinger, J.L.; Bonaccorso, R.L.; Borner, T.; Reiner, D.J.; Koch-Laskowski, K.; McGrath, L.E.; Lhamo, R.; Stein, L.M.; De Jonghe, B.C.; Holz, G.G.; Roth, C.L.; Doyle, R.P.; Hayes, M.R. A Vitamin B12 Conjugate of Exendin-4 Improves Glucose Tolerance Without Associated Nausea or Hypophagia in Rodents. Diabetes, Obesity & Metabolism, 2018, 20(5), 1223–1234. doi: 10.1111/dom.13222
- Sacoman, J.L.; Dagda, R.Y.; Burnham-Marusich, A.R.; Dagda, R.K.; Berninsone, P.M. Mitochondrial O-GlcNAc transferase (mOGT) regulates mitochondrial structure, function and survival in HeLa cells. Journal of Biological Chemistry, 2017, 292(11), 4499–4518. doi: 10.1074/jbc.M116.726752
- Wallat, J.D.; Czapar, A.E.; Wang, C.; Wen, A.M.; Wek, K.S.; Yu, X.; Steinmetz, N.F.; Pokorski, J.K. Optical and Magnetic Resonance Imaging Using Fluorous Colloidal Nanoparticles. Biomacromolecules, 2017, 18(1), 103–112. doi: 10.1021/acs.biomac.6b01389
- Zhao, T.; Li, T.; Liu, Y. Silver nanoparticle plasmonic enhanced förster resonance energy transfer (FRET) imaging of protein-specific sialylation on the cell surface. Nanoscale, 2017, 9(28), 9841–9847. doi: 10.1039/c7nr01562c
Cy® is a trademark of GE Healthcare.
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