DiR, lipophilic tracer
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
3433-1mg | 1 mg | – | in stock | |
3433-5mg | 5 mg | $140 | in stock | |
3433-50mg | 50 mg | please inquire | in stock |
DiR is a near-infrared fluorescent carbocyanine dye for cell membrane labeling in vivo and in vitro. DiR diffuses laterally to stain the entire cell, allowing it to be used as an anterograde and retrograde tracer of neurons. In intact tissue, the dye does not transfer from labeled to unlabeled cells, but some transfer may occur when the membrane is disrupted, for example, after sectioning. The dye is weakly fluorescent until incorporated into membranes.
DiR can be used with other tracers in multi-color studies, such as DiI or DiO and for NIR imaging.
Absorption and emission spectra of DiR
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Cyanine5 maleimide reacts with thiols to give dye labeled conjugates.General properties
Appearance: | violet-green sticky solid |
Molecular weight: | 1013.42 |
CAS number: | 100068-60-8 |
Molecular formula: | C63H101IN2 |
Solubility: | DMSO |
Quality control: | NMR 1H and HPLC-MS (95+%) |
Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 750 |
ε, L⋅mol−1⋅cm−1: | 254000 |
Emission maximum, nm: | 775 |
Fluorescence quantum yield: | 0.25 |
CF260: | 0.08 |
CF280: | 0.08 |
Product is offered and sold for research purposes only. Product is not tested for safety and efficacy in food, drug, medical device, cosmetic, no express or implied authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, for humans or animals or for commercial purposes.
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