Lumizol Reagent
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
91415 | 50 mL | $105 | in stock |
Lumizol reagent includes phenol, guanidine isothiocyanate and other components which makes the high-quality RNA extraction possible. Such components as phenol and guanidine isothiocyanate provide cell lysis and inhibit ribonucleases effectively. Thus the RNA is preserved intact.
After samples homogenization followed by the addition of chloroform the phase separation occurs: an upper, aqueous phase, the middle interphase and lower organic phase. RNA in the upper aqueous phase is then collected by precipitation with isopropanol, while DNA and proteins might be collected from the interphase and organic phase. DNA isolation from interphase and lower organic phase can be performed using ethanol solution and for proteins by precipitation with isopropanol from supernatant.
Recommended protocol
Calculator
Customers also purchased with this product
Cyanine3 azide
Azide derivative of Cyanine3 fluorescent dye for сlick сhemistry.ProteOrange Protein Gel Stain, 5000×
ProteOrange is a fluorescent dye for the staining of proteins in gels. ProteOrange is an analog of SYPRO orange. It is more sensitive than Coumassie.DBCO NHS ester
Azodibenzocyclooctyne (DBCO, ADIBO) reagent for strain-promoted copper-free click chemistry (SPAAC). The reagent contains an NHS ester function for the attachment of the cyclooctyne to various molecules.General properties
Appearance: | yellow-orange solution |
Quality control: | functional testing |
Storage conditions: | 12 months after receival at −20°C in the dark. Short-term storage at +4°C. Transportation: at room temperature for up to 3 weeks. |
MSDS: | Download |
Product specifications |
Product citations
- Borisenko, I.; Predeus, A.; Lavrov, A.; Ereskovsky, A. First draft genome assembly and characterization of spongeHalisarca dujardiniireveals key components of basement membrane and broad repertoire of aggregation factors. bioRxiv, 2024, preprint. doi: 10.1101/2024.02.06.578935