MitoCLox, mitochondrial lipid peroxidation probe

Cat. # Quantity Price Lead time
3549-50ug 50 ug –   in stock
3549-500ug 500 ug $260 in stock
3549-1mg 1 mg $495 in stock
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During the ferroptosis and mitochondrial stage of apoptosis, a mitochondria-specific phospholipid, cardiolipin (CL), undergoes peroxidation. MitoCLox is a mitochondria-targeted fluorescence probe that allows monitoring of this process in vivo.

MitoCLox consists of the BDP 581/591 fluorophore carrying a diene-containing moiety (C11) and linked with a triphenylphosphonium (TPP) residue via a long flexible linker with two amide bonds. MitoCLox is similar to MitoPerOx but has a longer linker and contains two (vs. one in MitoPerOx) peptide bonds. The flexible linker of MitoCLox mimics the SS-20 peptide (Phe-D-Arg-Phe-Lys-NH2), making the indicator specific for cardiolipin. The linker also increases the cellular permeability of MitoCLox due to additional positive charges.

The oxidation of the diene in MitoCLox results in a substantial increase in the fluorescence emission at 520 nm and a decrease in the initial fluorescence at 590 nm of the BDP 581/591 fluorophore. Thus, the oxidation of MitoCLox could be measured either as a decrease of absorbance at 588 nm or as an increase of fluorescence emission in the ratiometric mode at 520/590 nm [1].

MitoCLox is accumulated in the mitochondria of living cells. Maximal accumulation of MitoCLox in the cells is reached in 45-60 min. After removing MitoCLox from the medium, the fluorescence of the cells slowly decreased and reached 50% of the maximum in approximately 1 h. The recommended working concentration of MitoCLox is 100-200 nM [2].

[1] Lyamzaev K.G. et al. MitoCLox: A Novel Mitochondria-Targeted Fluorescent Probe for Tracing Lipid Peroxidation. Oxid. Med. Cell Longev. 2019:9710208.

[2] Lyamzaev K.G. et al. Novel Fluorescent Mitochondria-Targeted Probe MitoCLox Reports Lipid Peroxidation in Response to Oxidative Stress In Vivo. Oxid. Med. Cell Longev. 2020:3631272.

Absorption and emission spectra of MitoCLox in oxidized form

Absorption and emission spectra of MitoCLox in oxidized form

Absorption and emission spectra of MitoCLox

Absorption and emission spectra of MitoCLox

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General properties

Appearance: black powder
Molecular weight: 901.69
Molecular formula: C50H53BBrF2N4O2P
Solubility: good in DMSO
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 585
ε, L⋅mol−1⋅cm−1: 138500
Emission maximum, nm: 595
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