YODi-3, red fluorescent nucleic acid stain
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YODi-3 (Oxazole Red Homodimer, also known as YOYO® -3) is a red fluorescent carbocyanine dimeric dye. YODi-3 is a cell-impermeant nucleic acid stain that is nonfluorescent in the absence of nucleic acids but exhibits significant fluorescence enhancement upon binding to DNA.
The bright fluorescence signal and low background make YODi-3 ideal for staining nucleic acids on microarrays, as well as for nuclear and chromosome counterstaining in multicolor fluorescence labeling experiments.
Simultaneous labeling with cell-impermeant YODi-3 and cell-permeable LUCS 13 or Annexin V-AF488 can be used to assess cell viability and apoptosis.
Absorption and emission spectra of YODi-3 (DNA–dye complex)
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General properties
Appearance:
dark violet solution
Molecular weight:
1322.73
CAS number:
156312-20-8
Molecular formula:
C53 H62 I4 N6 O2
Quality control:
NMR 1 H and HPLC-MS (95+%)
Storage conditions:
24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS:
Download
Product specifications
Spectral properties
Excitation/absorption maximum, nm:
612
Emission maximum, nm:
631
YOYO® is the trademark of Molecular Probes.
Product is offered and sold for research purposes only. Product is not tested for safety and efficacy in food, drug, medical device, cosmetic, no express or implied authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, for humans or animals or for commercial purposes.
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