FAM-11-dUTP, 6-isomer

Cat. # Quantity Price Lead time
2077-50nmol 50 nmol $190.00 in stock
2077-250nmol 250 nmol $750.00 in stock
2077-500nmol 500 nmol $1200.00 7 days
2077-1umol 1 umol please inquire in stock

6-FAM-11-2’-deoxyuridine-5’-triphosphate, trilithium salt, is a common agent for non-radioactive DNA labeling.

FAM (fluorescein) is a popular green-fluorescent dye with an emission maximum at 513 nm. This derivative is a pure FAM 6-isomer.

6-FAM-11-dUTP is a modified triphosphate that can be incorporated into DNA instead of dTTP by various enzymes, including Klenow fragment, Taq-Polymerase, DNA-Polymerase I, Phi 29 Polymerase, reverse transcriptase or terminal transferase.

In contrast to triphosphates with a dye directly linked to aminoallyl-dUTP, this triphosphate contains a linker of 11 atoms between the fluorophore and the nitrogenous base. This linker length prevents FAM-label from potential static quenching and increases the efficiency of nucleotide incorporation during DNA synthesis.

6-FAM-11-dUTP can be used to produce a labeled product during PCR and cDNA synthesis and for labeled DNA-probes synthesis by random primed labeling and Nick-translation. Synthesized fluorescence-labeled DNA probes can be used to detect specific sequences by Southern blot, Northern blot, in situ hybridization, or by microarray analysis.

Absorption and emission spectra of FAM

Absorption and emission spectra of FAM

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General properties

Appearance: yellow-greenish solid
Molecular weight: 1013.49
Molecular formula: C39H39N4Li3O21P3
IUPAC name: ((2R,3S,5R)-5-(5-(3-(6-(3-carboxy-4-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzamido)hexanamido)prop-1-yn-1-yl)-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl hydrogen triphosphate
Solubility: soluble in water
Quality control: HPLC-MS (95%), testing in enzymatic reaction
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light and excessive freeze-thaw cycles.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 490
ε, L⋅mol−1⋅cm−1: 80000
Emission maximum, nm: 513
Fluorescence quantum yield: 0.93
CF260: 0.20
CF280: 0.17
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